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The map label for this gene is emrD [C]

Identifier: 116874138

GI number: 116874138

Start: 2758195

End: 2759373

Strand: Direct

Name: emrD [C]

Synonym: lwe2722

Alternate gene names: 116874138

Gene position: 2758195-2759373 (Clockwise)

Preceding gene: 116874137

Following gene: 116874163

Centisome position: 98.01

GC content: 36.98

Gene sequence:

>1179_bases
ATGGAAAAAAACATCGAAACAAAGAAAATCAATTTGGCGCTACTCGTTTGTCTTGTTGGTTTTCCGCAAATTAGTGAAAC
TATTTATACACCTTCTTTGACTGAAATTGCCACAAGCTATGGTATCTCATTAAATCTGGCTCAGATGACTTTAAGCATCT
ACTTTCTTGCTTTTGCCGCTGGTGTATTCTTTTGGGGAGTGAGTTCAGATTATCTAGGGCGACGTAAAGCCATGAACTTT
GGGATATTTATTTATATTCTTGGGTGCCTGGTTTGCCTTATTTCTAGTAATATTACAGTGCTTTTTATCGGCCGTTTTAT
TCAAGCTTTTGGTGCTAGTACGGGTTCTGTTACAACACAAACAATTTTACGAGATAATTATCATGGTAAAAATCGCCATC
AGCTCTTTGCAAAAATATCAGCTGCCTTAGCATTTTCACCCGCAATCGGTCCATTAGTTGGTGGATTTATCGGACAATTT
TACGGCTTTCGCGCAGTATTTTTATGTTTAGTAATTATGGGGATGGCGCTGTTTTTCTGGAGTTTAAAACGATTGCCAGA
AACTAACACTGTTACAACTACTTCGTTTAGCGTACAGGAAATGCTAGTAATTGGTAAAAAAATGATTTATAACCGCTATA
CCACTTCTTTTGGTGTATTAATTGGGATTTTCAATGGAATTTTGTTTAGTTACTATTCAGAAGCACCAACTATTTTCATG
GAGAAGTTTCATTTTAGCCAGAGTCAGTATGGCTTTATGGGGTGTGCTGTGGCCGCAGCAACGATACTGGGCGCTTGGCT
CTCTAATCTGCGCTTAAAACAGAATTCGCCACATAAAATAATTAAAGAAGGGATTTTACTAGCCCTGTTTGGCACTACAG
CCCTTAGCACAGTTGCCCTACTCCCGCTAAAAATCCAAGTAATAATGTATATTCTCTTCATTTCTATTATCTTAGTAGGA
ATCGGCATGGCACTACCAAATTGTTTAAGCTTAGCATTAGTAAAATTTCAAGAAGTTGCTGGAACGGCTGGTGCCTTTTT
AAGCTTAGGATACTATGTAATTGTCAGTATTTGTACGTTTTTAATCGGAGTTTTTCACACCGGATCCATACTTGTATTTC
CAATATTTTGTATCGTATTGCTTCTAATTGCGCTTATTTGTATAAAATTTAGTAAATAG

Upstream 100 bases:

>100_bases
CTACATGAATTTTTATGAGCGTATTAAAATCATGGTCCCACCATTTCCCTGTAAAGGATGTTTTTTTCCCAGATAAATAA
ATAATTCAGGAGAGTTTTTT

Downstream 100 bases:

>100_bases
AAAACAGGAAACTTTTTATAGCCATTTGCGAAAAAAGATGGCTTAAGAGAGTTTCCTGTTTATTTTAAAGTTAAGTTAGT
TGGATTTTTTATATGATTTT

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MEKNIETKKINLALLVCLVGFPQISETIYTPSLTEIATSYGISLNLAQMTLSIYFLAFAAGVFFWGVSSDYLGRRKAMNF
GIFIYILGCLVCLISSNITVLFIGRFIQAFGASTGSVTTQTILRDNYHGKNRHQLFAKISAALAFSPAIGPLVGGFIGQF
YGFRAVFLCLVIMGMALFFWSLKRLPETNTVTTTSFSVQEMLVIGKKMIYNRYTTSFGVLIGIFNGILFSYYSEAPTIFM
EKFHFSQSQYGFMGCAVAAATILGAWLSNLRLKQNSPHKIIKEGILLALFGTTALSTVALLPLKIQVIMYILFISIILVG
IGMALPNCLSLALVKFQEVAGTAGAFLSLGYYVIVSICTFLIGVFHTGSILVFPIFCIVLLLIALICIKFSK

Sequences:

>Translated_392_residues
MEKNIETKKINLALLVCLVGFPQISETIYTPSLTEIATSYGISLNLAQMTLSIYFLAFAAGVFFWGVSSDYLGRRKAMNF
GIFIYILGCLVCLISSNITVLFIGRFIQAFGASTGSVTTQTILRDNYHGKNRHQLFAKISAALAFSPAIGPLVGGFIGQF
YGFRAVFLCLVIMGMALFFWSLKRLPETNTVTTTSFSVQEMLVIGKKMIYNRYTTSFGVLIGIFNGILFSYYSEAPTIFM
EKFHFSQSQYGFMGCAVAAATILGAWLSNLRLKQNSPHKIIKEGILLALFGTTALSTVALLPLKIQVIMYILFISIILVG
IGMALPNCLSLALVKFQEVAGTAGAFLSLGYYVIVSICTFLIGVFHTGSILVFPIFCIVLLLIALICIKFSK
>Mature_392_residues
MEKNIETKKINLALLVCLVGFPQISETIYTPSLTEIATSYGISLNLAQMTLSIYFLAFAAGVFFWGVSSDYLGRRKAMNF
GIFIYILGCLVCLISSNITVLFIGRFIQAFGASTGSVTTQTILRDNYHGKNRHQLFAKISAALAFSPAIGPLVGGFIGQF
YGFRAVFLCLVIMGMALFFWSLKRLPETNTVTTTSFSVQEMLVIGKKMIYNRYTTSFGVLIGIFNGILFSYYSEAPTIFM
EKFHFSQSQYGFMGCAVAAATILGAWLSNLRLKQNSPHKIIKEGILLALFGTTALSTVALLPLKIQVIMYILFISIILVG
IGMALPNCLSLALVKFQEVAGTAGAFLSLGYYVIVSICTFLIGVFHTGSILVFPIFCIVLLLIALICIKFSK

Specific function: Multidrug Resistance Pump That Participates In A Low Energy Shock Adaptative Response. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI87082312, Length=170, Percent_Identity=28.2352941176471, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1790794, Length=339, Percent_Identity=23.3038348082596, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI48994889, Length=353, Percent_Identity=23.5127478753541, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790146, Length=225, Percent_Identity=23.1111111111111, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788509, Length=233, Percent_Identity=23.175965665236, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787065, Length=267, Percent_Identity=21.3483146067416, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43000; Mature: 43000

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA