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The map label for this gene is mrpA [H]

Identifier: 116873743

GI number: 116873743

Start: 2373145

End: 2375553

Strand: Direct

Name: mrpA [H]

Synonym: lwe2327

Alternate gene names: 116873743

Gene position: 2373145-2375553 (Clockwise)

Preceding gene: 116873742

Following gene: 116873744

Centisome position: 84.33

GC content: 37.19

Gene sequence:

>2409_bases
TTGTCATTTCTTCATTTAGCAATTCTTCTGCCGTTTATTGTGGCGCTGCTAATTCCACTATTTTATCGGTGGACTAAACA
AGTTCATACTGGCTGGTTAGTACTTCCAATTCCAGTGATATTGTTTATTTACTTCTTAACTTTTATTCCAAAGACTATGC
ACGAGGCAACAATTGTATCAATGCCTTGGATTCCACGACTTGGAATTAATTTTACAGTAGTGGTTGATGGGCTAAGTTTA
CTATTTGCACTACTTATTACTGGAATTGGTTCACTCGTTACTTTTTACTCTATTTATTATTTAGGGAAAAAGAAAGAACG
ACTTAGCAATTTCTATACATTTTTATTTATGTTTATGACCGCAATGCTCGGGGTTGTCTTGAGTGATAACTTGATTGTGT
TGTATCTTTTCTGGGAGCTTACTTCGATTAGTTCGTTCTTATTGATTGGCTATTGGTACCATCGTGAGCGTTCACGTTAT
GGAGCTCGTAAATCGATGATGATTACTGTATTTGGCGGACTAATGATGCTTGGCGGTTTTATTTTACTTCATATTATGAG
TGATTCTTTTTCTATTCGTGCCATCATTCAAAACGCGGATATAATTAGTCACAACAGTTTATTCATTCCAGCAATGATTC
TCGTCTTGCTTGGTGCATTTACGAAATCTGCACAAGTGCCATTCCATATTTGGCTTCCAGACGCAATGGAAGCACCTACT
CCGGTGAGCGCATATCTTCACTCTGCTACGATGGTTAAAGCTGGGATTTATATTGTGGCTCGTTTTACACCTCTATTCGC
AAGTTCAGGTGTTTGGTTTTGGACGGTATCACTCGTTGGGATTACAACGCTTTTCTGGGGTTCACTTAATGCTACGAAGA
AAAATGATTTAAAAGCGATTTTGGCTTACTCGACAATTAGTCAACTCGGTATGATTATGGCTCTTCTTGGTGTCGGAGCT
GCCTCGCTTCATTTTGATACGCTTAGTGATGACATTTATGTGATGGCTATTGTGGCAGCTGTATTCCATCTATTTAATCA
TGCAACATTTAAAGGTAGCTTGTTTATGATGGTCGGTATTGTCGATCACGAAACTGGCACTCGGGATATTAGACGACTAG
GCGGCTTAATGCGTATCATGCCTATTACAGCGACAATTGCATTTATCGGAACTTTTGCAATGGCCGGGATTCCCCCTTTT
AATGGATTTTTAAGTAAAGAAATGTTTTTTGAAAGCATGATAAATATTACGCATTTGCAGTTATTTGATGCAAGTACTTG
GGGCATTTTACTTCCAGTTGTGGCTTGGGTTGCTAGTGTATTTACATTTGTTTATAGTATGATAATTTTCTTCAAAACAT
TCACTGGAAAAGTGAAGCCTTTCTTACTTCCGAAAAAACCTCATGAAGCACCATTCGGGCTGCTTTTACCACCTATTATT
TTATCCATTTTTGTTGTATTGATTGGCTTATTCCCTAATTTAATTGCGAAGCCGATTTTGGAACCAGCAGTAAGAGCAAT
TGTTCCAGGACTAGCTACTGATTTTTCTATTCATATTAGTTTATGGCATGGCTTTACACCTGCTTTATTGATGACGTTTG
GCGTTGTTGCTGTTGGAATTTTGTTGTTCCTTACACACAAATACTGGAAACCTTGGATTACTACCCGAGTTCCAAAAGCA
CTACGTATTGGAAAAACATATGATAATGGGATGTACTATTTAGAGCAAGGTTCCTACCGGATGACCATGTTTATTATGAC
TGGTTGGCTCAGAACTTACTTAAATTATATGTTATCCGCTTTTATCTTAATGATGGCTTCGGTGATGATTTTTACAAATG
CACTTGATTTCAACTTTTCGAGTATGACAAAAGTGACAGTCGTTGATTTTGTTTTAGCAGCAGTTATTTTAGTAACTTTA
GTTGGAATTGTCTTTTCAAAATCGCGTATTACATCCATTATTTTGCTTGGAGCGATGGGCTATACGATTAGTATTTTCTT
CGTTATTTCAAGAGCGCCTGACCTTGCTTTGACACAACTTATTATTGAAACTATTTCCGTTGTGCTATATCTACTTGTGT
TCTATCATCTTCCCCAATTCAGTAATATTGAGGAAAAGCCGAAATGGTTATCAATGAAGACATTTTTGAGTATCGGGGTT
GGAATTATTATTACGCTGGTTTCGCTATCGGCTTATAATACGTCGTTCTATGATTCTATTTCGAAATACTATGTGGATAA
CGCATATGTAGAAGCAGCTGGACGTAATATTGTTAATGTTATCTTAGTGGACTTCCGTGGCTTTGATACAATGTTTGAAA
CAGCTGTACTATCTATTGCCGCAATTGGTATTTATGCGATGATAAAATTACGACTAACGAAACGAGGTGAGAATGATGAA
AACGAATGA

Upstream 100 bases:

>100_bases
AAATGTAAGTACTTGCTTAAAAAATAATGACTTCCCTATTCTTATTTTCACATTTCCAGGATGAGTTTCCGTATAAAAAT
TCTTCGAGGAGGAGCCGATT

Downstream 100 bases:

>100_bases
CGTTCTACTTAGGAATGTGACGAAAGTAGTAGCTTTCATTATTTTCTTATTCTCACTTCACCTATTCTTTGCAGGACACT
ATAATCCTGGCGGCGGGTTT

Product: monovalent cation/H+ antiporter subunit A

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 802; Mature: 801

Protein sequence:

>802_residues
MSFLHLAILLPFIVALLIPLFYRWTKQVHTGWLVLPIPVILFIYFLTFIPKTMHEATIVSMPWIPRLGINFTVVVDGLSL
LFALLITGIGSLVTFYSIYYLGKKKERLSNFYTFLFMFMTAMLGVVLSDNLIVLYLFWELTSISSFLLIGYWYHRERSRY
GARKSMMITVFGGLMMLGGFILLHIMSDSFSIRAIIQNADIISHNSLFIPAMILVLLGAFTKSAQVPFHIWLPDAMEAPT
PVSAYLHSATMVKAGIYIVARFTPLFASSGVWFWTVSLVGITTLFWGSLNATKKNDLKAILAYSTISQLGMIMALLGVGA
ASLHFDTLSDDIYVMAIVAAVFHLFNHATFKGSLFMMVGIVDHETGTRDIRRLGGLMRIMPITATIAFIGTFAMAGIPPF
NGFLSKEMFFESMINITHLQLFDASTWGILLPVVAWVASVFTFVYSMIIFFKTFTGKVKPFLLPKKPHEAPFGLLLPPII
LSIFVVLIGLFPNLIAKPILEPAVRAIVPGLATDFSIHISLWHGFTPALLMTFGVVAVGILLFLTHKYWKPWITTRVPKA
LRIGKTYDNGMYYLEQGSYRMTMFIMTGWLRTYLNYMLSAFILMMASVMIFTNALDFNFSSMTKVTVVDFVLAAVILVTL
VGIVFSKSRITSIILLGAMGYTISIFFVISRAPDLALTQLIIETISVVLYLLVFYHLPQFSNIEEKPKWLSMKTFLSIGV
GIIITLVSLSAYNTSFYDSISKYYVDNAYVEAAGRNIVNVILVDFRGFDTMFETAVLSIAAIGIYAMIKLRLTKRGENDE
NE

Sequences:

>Translated_802_residues
MSFLHLAILLPFIVALLIPLFYRWTKQVHTGWLVLPIPVILFIYFLTFIPKTMHEATIVSMPWIPRLGINFTVVVDGLSL
LFALLITGIGSLVTFYSIYYLGKKKERLSNFYTFLFMFMTAMLGVVLSDNLIVLYLFWELTSISSFLLIGYWYHRERSRY
GARKSMMITVFGGLMMLGGFILLHIMSDSFSIRAIIQNADIISHNSLFIPAMILVLLGAFTKSAQVPFHIWLPDAMEAPT
PVSAYLHSATMVKAGIYIVARFTPLFASSGVWFWTVSLVGITTLFWGSLNATKKNDLKAILAYSTISQLGMIMALLGVGA
ASLHFDTLSDDIYVMAIVAAVFHLFNHATFKGSLFMMVGIVDHETGTRDIRRLGGLMRIMPITATIAFIGTFAMAGIPPF
NGFLSKEMFFESMINITHLQLFDASTWGILLPVVAWVASVFTFVYSMIIFFKTFTGKVKPFLLPKKPHEAPFGLLLPPII
LSIFVVLIGLFPNLIAKPILEPAVRAIVPGLATDFSIHISLWHGFTPALLMTFGVVAVGILLFLTHKYWKPWITTRVPKA
LRIGKTYDNGMYYLEQGSYRMTMFIMTGWLRTYLNYMLSAFILMMASVMIFTNALDFNFSSMTKVTVVDFVLAAVILVTL
VGIVFSKSRITSIILLGAMGYTISIFFVISRAPDLALTQLIIETISVVLYLLVFYHLPQFSNIEEKPKWLSMKTFLSIGV
GIIITLVSLSAYNTSFYDSISKYYVDNAYVEAAGRNIVNVILVDFRGFDTMFETAVLSIAAIGIYAMIKLRLTKRGENDE
NE
>Mature_801_residues
SFLHLAILLPFIVALLIPLFYRWTKQVHTGWLVLPIPVILFIYFLTFIPKTMHEATIVSMPWIPRLGINFTVVVDGLSLL
FALLITGIGSLVTFYSIYYLGKKKERLSNFYTFLFMFMTAMLGVVLSDNLIVLYLFWELTSISSFLLIGYWYHRERSRYG
ARKSMMITVFGGLMMLGGFILLHIMSDSFSIRAIIQNADIISHNSLFIPAMILVLLGAFTKSAQVPFHIWLPDAMEAPTP
VSAYLHSATMVKAGIYIVARFTPLFASSGVWFWTVSLVGITTLFWGSLNATKKNDLKAILAYSTISQLGMIMALLGVGAA
SLHFDTLSDDIYVMAIVAAVFHLFNHATFKGSLFMMVGIVDHETGTRDIRRLGGLMRIMPITATIAFIGTFAMAGIPPFN
GFLSKEMFFESMINITHLQLFDASTWGILLPVVAWVASVFTFVYSMIIFFKTFTGKVKPFLLPKKPHEAPFGLLLPPIIL
SIFVVLIGLFPNLIAKPILEPAVRAIVPGLATDFSIHISLWHGFTPALLMTFGVVAVGILLFLTHKYWKPWITTRVPKAL
RIGKTYDNGMYYLEQGSYRMTMFIMTGWLRTYLNYMLSAFILMMASVMIFTNALDFNFSSMTKVTVVDFVLAAVILVTLV
GIVFSKSRITSIILLGAMGYTISIFFVISRAPDLALTQLIIETISVVLYLLVFYHLPQFSNIEEKPKWLSMKTFLSIGVG
IIITLVSLSAYNTSFYDSISKYYVDNAYVEAAGRNIVNVILVDFRGFDTMFETAVLSIAAIGIYAMIKLRLTKRGENDEN
E

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=368, Percent_Identity=35.054347826087, Blast_Score=189, Evalue=8e-48,
Organism=Escherichia coli, GI1788614, Length=561, Percent_Identity=31.5508021390374, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1788829, Length=479, Percent_Identity=31.5240083507307, Blast_Score=180, Evalue=4e-46,
Organism=Escherichia coli, GI1788831, Length=362, Percent_Identity=32.0441988950276, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI1788827, Length=445, Percent_Identity=26.2921348314607, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI1788613, Length=427, Percent_Identity=27.4004683840749, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI2367154, Length=288, Percent_Identity=29.1666666666667, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI145693160, Length=342, Percent_Identity=26.0233918128655, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 89996; Mature: 89865

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLHLAILLPFIVALLIPLFYRWTKQVHTGWLVLPIPVILFIYFLTFIPKTMHEATIVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHEEEE
MPWIPRLGINFTVVVDGLSLLFALLITGIGSLVTFYSIYYLGKKKERLSNFYTFLFMFMT
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AMLGVVLSDNLIVLYLFWELTSISSFLLIGYWYHRERSRYGARKSMMITVFGGLMMLGGF
HHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ILLHIMSDSFSIRAIIQNADIISHNSLFIPAMILVLLGAFTKSAQVPFHIWLPDAMEAPT
HHHHHHCCCCEEEEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
PVSAYLHSATMVKAGIYIVARFTPLFASSGVWFWTVSLVGITTLFWGSLNATKKNDLKAI
CHHHHHHHHHHHHHCEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LAYSTISQLGMIMALLGVGAASLHFDTLSDDIYVMAIVAAVFHLFNHATFKGSLFMMVGI
HHHHHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
VDHETGTRDIRRLGGLMRIMPITATIAFIGTFAMAGIPPFNGFLSKEMFFESMINITHLQ
EECCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEE
LFDASTWGILLPVVAWVASVFTFVYSMIIFFKTFTGKVKPFLLPKKPHEAPFGLLLPPII
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHH
LSIFVVLIGLFPNLIAKPILEPAVRAIVPGLATDFSIHISLWHGFTPALLMTFGVVAVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHH
LLFLTHKYWKPWITTRVPKALRIGKTYDNGMYYLEQGSYRMTMFIMTGWLRTYLNYMLSA
HHHHHHHHCCCHHHHCCCHHHHCCCEECCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHH
FILMMASVMIFTNALDFNFSSMTKVTVVDFVLAAVILVTLVGIVFSKSRITSIILLGAMG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTISIFFVISRAPDLALTQLIIETISVVLYLLVFYHLPQFSNIEEKPKWLSMKTFLSIGV
HHHEEHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
GIIITLVSLSAYNTSFYDSISKYYVDNAYVEAAGRNIVNVILVDFRGFDTMFETAVLSIA
HHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHH
AIGIYAMIKLRLTKRGENDENE
HHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SFLHLAILLPFIVALLIPLFYRWTKQVHTGWLVLPIPVILFIYFLTFIPKTMHEATIVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHEEEE
MPWIPRLGINFTVVVDGLSLLFALLITGIGSLVTFYSIYYLGKKKERLSNFYTFLFMFMT
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AMLGVVLSDNLIVLYLFWELTSISSFLLIGYWYHRERSRYGARKSMMITVFGGLMMLGGF
HHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ILLHIMSDSFSIRAIIQNADIISHNSLFIPAMILVLLGAFTKSAQVPFHIWLPDAMEAPT
HHHHHHCCCCEEEEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
PVSAYLHSATMVKAGIYIVARFTPLFASSGVWFWTVSLVGITTLFWGSLNATKKNDLKAI
CHHHHHHHHHHHHHCEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LAYSTISQLGMIMALLGVGAASLHFDTLSDDIYVMAIVAAVFHLFNHATFKGSLFMMVGI
HHHHHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
VDHETGTRDIRRLGGLMRIMPITATIAFIGTFAMAGIPPFNGFLSKEMFFESMINITHLQ
EECCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEE
LFDASTWGILLPVVAWVASVFTFVYSMIIFFKTFTGKVKPFLLPKKPHEAPFGLLLPPII
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHH
LSIFVVLIGLFPNLIAKPILEPAVRAIVPGLATDFSIHISLWHGFTPALLMTFGVVAVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHH
LLFLTHKYWKPWITTRVPKALRIGKTYDNGMYYLEQGSYRMTMFIMTGWLRTYLNYMLSA
HHHHHHHHCCCHHHHCCCHHHHCCCEECCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHH
FILMMASVMIFTNALDFNFSSMTKVTVVDFVLAAVILVTLVGIVFSKSRITSIILLGAMG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTISIFFVISRAPDLALTQLIIETISVVLYLLVFYHLPQFSNIEEKPKWLSMKTFLSIGV
HHHEEHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
GIIITLVSLSAYNTSFYDSISKYYVDNAYVEAAGRNIVNVILVDFRGFDTMFETAVLSIA
HHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHH
AIGIYAMIKLRLTKRGENDENE
HHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]