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The map label for this gene is opuD [H]

Identifier: 116873529

GI number: 116873529

Start: 2145322

End: 2146845

Strand: Direct

Name: opuD [H]

Synonym: lwe2113

Alternate gene names: 116873529

Gene position: 2145322-2146845 (Clockwise)

Preceding gene: 116873528

Following gene: 116873538

Centisome position: 76.23

GC content: 37.93

Gene sequence:

>1524_bases
TTGAAAAAATTAACAACTGTTTTTTGGGGTTCGGGACTATTAGTTCTGCTAGCTGTTTTATTTGGTGCTTTTTTACCAAA
ACAATTTGAGAATTTCACAACAAACATTCAACAATTTCTAACAAGTAATTTTGGTTGGTACTATTTATTTGTGGTTGCGA
TTATTATTATCTTTTGTTTATTCTTAGTTTTAAGCCCAATTGGTTCGATTCGTCTTGGAAAGCCCGGGGAAAAACCAGGT
TATAGTAATAAATCATGGTTCGCGATGTTATTCAGCGCAGGAATGGGAATCGGACTTGTTTTCTGGGGTGCTGCCGAGCC
ATTATCTCACTATGCTGTTCAAGCGCCTGGTGGTGAAGTTGGTACGCAAGCTGCTATGAAAGATGCGCTTCGTTATTCAT
TCTTCCATTGGGGGATATCTGCTTGGTCCATTTATGCGATTGTGGCTTTAGCCTTAGCTTACTTTAAATTCAGGAAAAAT
GCGCCTGGTTTAATAAGCGCTACGCTATATCCAATTTTAGGAAAATATTCAAAGGGTCCTCTTGGTCAGTTGATTGATAT
TATTGCTGTTTTTGCAACGGTTATTGGAGTTGCTACAACTCTTGGCCTTGGTGCTCAACAGATTAATGGTGGGCTAACTT
ACTTATTCGGCGTTCCAAATAACTTTGGGATTCAATTTACTATAATTGTTATTGTTACTATTTTATTCATGCTATCCGCA
ATGTCAGGACTTGATAAAGGGATTCAGCTTTTAAGTAATGTAAATATATATGTTGCTGGTGTTTTACTGGTTCTTACACT
TATTCTTGGACCGACTCTCTTCATTATGAATAATTTTACGAATTCCTTTGGGGATTACTTGCAAAATATTATACAGATGA
GTTTCCAAACAGCACCTGATGCTCCTGATGCTAGAAAATGGATTGATTCCTGGACTATTTTTTACTGGGCTTGGTGGCTT
TCTTGGTCGCCATTTGTTGGGATATTTATTGCACGTATTTCCCGCGGCAGAACAATCCGGCAGTTTTTACTAGGAGTTAT
CGTTTTACCTGCTCTAGTAAGTGTGTTTTGGTTTGCGGTATTTGGCGGCTCTGCAATTTTTGTGGAACAACATGGGAATT
CAGGACTTTCAAAACTAGCAACTGAACAAGTATTATTTGGGGTCTTTAATGAATTTCCGGCTGGAATGGTTTTATCGATT
GTTGCAATGATTTTAATTGCCGTATTCTTTATCACTTCTGCGGATTCTGCGACATTTGTGCTTGGTATGCAAACGACAGG
CGGTTCTTTAAACCCACCAAATTCGGTCAAAGTCACTTGGGGACTTTTGCAAGCTGGAATTGCGAGTGTTTTACTTTATG
CTGGTGGACTTACAGCTCTACAAAACGCTTCGATTATTGCGGCTTTCCCATTTTCAATTGTTATTATTTTAATGATTGTT
TCCTTATTTATTTCACTGACGAGAGAACGAGAAAAATTAGGATTATTTGTACGACCGAAAAAATCACAACGTTCACAACT
ATAA

Upstream 100 bases:

>100_bases
GGATTGTCAAACCACTGTTTTTTTTGTTAATATAAAAAAGATGTTACCTTTTTGCTAGATAGGGGAAATACATACAGAGA
AAATAAAGGGAAGTGATGTA

Downstream 100 bases:

>100_bases
TAAAAAGGGATGGAAATCTAAAATTAGATTTCCATCCCTTTTTTCATCCTTTTTTCTGCTTTGCCGCGATTATTTCACGA
TAGCTACGAGTTTTTTTAGG

Product: glycine betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MKKLTTVFWGSGLLVLLAVLFGAFLPKQFENFTTNIQQFLTSNFGWYYLFVVAIIIIFCLFLVLSPIGSIRLGKPGEKPG
YSNKSWFAMLFSAGMGIGLVFWGAAEPLSHYAVQAPGGEVGTQAAMKDALRYSFFHWGISAWSIYAIVALALAYFKFRKN
APGLISATLYPILGKYSKGPLGQLIDIIAVFATVIGVATTLGLGAQQINGGLTYLFGVPNNFGIQFTIIVIVTILFMLSA
MSGLDKGIQLLSNVNIYVAGVLLVLTLILGPTLFIMNNFTNSFGDYLQNIIQMSFQTAPDAPDARKWIDSWTIFYWAWWL
SWSPFVGIFIARISRGRTIRQFLLGVIVLPALVSVFWFAVFGGSAIFVEQHGNSGLSKLATEQVLFGVFNEFPAGMVLSI
VAMILIAVFFITSADSATFVLGMQTTGGSLNPPNSVKVTWGLLQAGIASVLLYAGGLTALQNASIIAAFPFSIVIILMIV
SLFISLTREREKLGLFVRPKKSQRSQL

Sequences:

>Translated_507_residues
MKKLTTVFWGSGLLVLLAVLFGAFLPKQFENFTTNIQQFLTSNFGWYYLFVVAIIIIFCLFLVLSPIGSIRLGKPGEKPG
YSNKSWFAMLFSAGMGIGLVFWGAAEPLSHYAVQAPGGEVGTQAAMKDALRYSFFHWGISAWSIYAIVALALAYFKFRKN
APGLISATLYPILGKYSKGPLGQLIDIIAVFATVIGVATTLGLGAQQINGGLTYLFGVPNNFGIQFTIIVIVTILFMLSA
MSGLDKGIQLLSNVNIYVAGVLLVLTLILGPTLFIMNNFTNSFGDYLQNIIQMSFQTAPDAPDARKWIDSWTIFYWAWWL
SWSPFVGIFIARISRGRTIRQFLLGVIVLPALVSVFWFAVFGGSAIFVEQHGNSGLSKLATEQVLFGVFNEFPAGMVLSI
VAMILIAVFFITSADSATFVLGMQTTGGSLNPPNSVKVTWGLLQAGIASVLLYAGGLTALQNASIIAAFPFSIVIILMIV
SLFISLTREREKLGLFVRPKKSQRSQL
>Mature_507_residues
MKKLTTVFWGSGLLVLLAVLFGAFLPKQFENFTTNIQQFLTSNFGWYYLFVVAIIIIFCLFLVLSPIGSIRLGKPGEKPG
YSNKSWFAMLFSAGMGIGLVFWGAAEPLSHYAVQAPGGEVGTQAAMKDALRYSFFHWGISAWSIYAIVALALAYFKFRKN
APGLISATLYPILGKYSKGPLGQLIDIIAVFATVIGVATTLGLGAQQINGGLTYLFGVPNNFGIQFTIIVIVTILFMLSA
MSGLDKGIQLLSNVNIYVAGVLLVLTLILGPTLFIMNNFTNSFGDYLQNIIQMSFQTAPDAPDARKWIDSWTIFYWAWWL
SWSPFVGIFIARISRGRTIRQFLLGVIVLPALVSVFWFAVFGGSAIFVEQHGNSGLSKLATEQVLFGVFNEFPAGMVLSI
VAMILIAVFFITSADSATFVLGMQTTGGSLNPPNSVKVTWGLLQAGIASVLLYAGGLTALQNASIIAAFPFSIVIILMIV
SLFISLTREREKLGLFVRPKKSQRSQL

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=477, Percent_Identity=39.4129979035639, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI1788102, Length=443, Percent_Identity=33.4085778781038, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI1786224, Length=465, Percent_Identity=30.9677419354839, Blast_Score=203, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 55297; Mature: 55297

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCEEECCCHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHCCEEECCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]