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The map label for this gene is ilvA [H]

Identifier: 116873426

GI number: 116873426

Start: 2038379

End: 2039647

Strand: Direct

Name: ilvA [H]

Synonym: lwe2010

Alternate gene names: 116873426

Gene position: 2038379-2039647 (Clockwise)

Preceding gene: 116873425

Following gene: 116873427

Centisome position: 72.43

GC content: 39.8

Gene sequence:

>1269_bases
ATGGAGTTAGTTGATTTATTAGTAACAGAAGAAGATGTCGAAAAAGCCTATGATGCATTAAAATCTGTCGTCAAACATAC
GCCACTGGAATATGATTTTTATCTTTCAGAAAAATATCATTGCAATGTGTACCTTAAACGTGAAGATTTACAGCGGGTTC
GTTCGTTTAAATTGCGTGGTGCTTTTTATGCTATTTCTAGATTATCAGCAGCACAACTAGAAAAAGGAGTAGCGTGTGCA
AGTGCTGGAAATCATGCCCAAGGAGTCGCTTACACTTGTAAACGAATGACAGTTCCTGCAACGATTTTTATGCCAACAAC
TACCCCACAACAAAAAGTATCTCAAGTAAGGTTTTTCGGTGGTAGTAACGTCGAAGTTGTTTTAGTTGGTGACACGTTTG
ATGCGTCAGCCACTGCTGCAAAAGAATACGCCGCACTTCATGGTCAGACTTTTATTCCTCCATTTGATGATCCAGATATT
ATTGCTGGTCAGGGATCGCTCGCTGTTGAAATGGTAAAAGATTTAAATAAAGCACATGAGCAAGCAGATTATGTTTTTGC
TGGCATTGGTGGTGGTGGTTTAATTAGTGGTGTTGCAACCTATCTTAAAGCGAAAAGCCCTATTACTAAAATTATCGGTG
TAGAGCCAGCTGGTGCCCCTTCCATGACTGAGGCTTTAAAACAAAACCAAGTCGTAACACTGGATAAAATCGATAAATTT
GTCGATGGCGCCGCAGTAAAAGAAGTGGGTAGTTTGACGTTTCAACATGCAAAAGTGTTGGTTGATGAAGTGGCAACGGT
GTCTGAAGGGGCGGTTTGTTCTACCATTTTAGATATGTATACGAAGCAAGCGATTGTTGCAGAACCAGCTGGCGCACTTT
CCGTGGCTGCTCTTGAAAAGTATCGCGAAGAAATTAAAGATAAAACGGTTGTTTGTATTGTTAGTGGCGGAAACAACGAT
ATCAACCGGATGCAAGAAATTGAAGAGCGTTCCCTTCTCCATGAAGGATTAAAGCATTATTTTATCGTCAATTTTTCGCA
ACGCCCTGGAGCTTTGAAAGAATTTGTGAACGATGTACTTGGCCCACATGATGATATCACTAAATTTGAATATACAAAAA
AGGTAAATCGCGGCAATGGACCTGTTATCATTGGTGTTTTATTGCAAGATAAAAATGATTACGAAGGCTTACTCGACCGG
GTTGCAGCTTTTGATCCCAACTATATTCCGATTAATGACAATCAAACCCTTTACACTTTACTTGTTTAA

Upstream 100 bases:

>100_bases
TAATGGACTGGATGATATCGCTATTACATTTGAACATATTGATGCGATTAAAGCCTATGAACAAAAAGTAGATTCCATAT
AAGAAAAGGTAGTGAGGATG

Downstream 100 bases:

>100_bases
AAAGAGTTCTCGCAGGACGCGGGATCTCTTTTTATATTACAGTTTGTCTGGCTGTTATAGCAGTATTTTATTTACGCTTA
TAGTTTAAAACCTTTTGTTT

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MELVDLLVTEEDVEKAYDALKSVVKHTPLEYDFYLSEKYHCNVYLKREDLQRVRSFKLRGAFYAISRLSAAQLEKGVACA
SAGNHAQGVAYTCKRMTVPATIFMPTTTPQQKVSQVRFFGGSNVEVVLVGDTFDASATAAKEYAALHGQTFIPPFDDPDI
IAGQGSLAVEMVKDLNKAHEQADYVFAGIGGGGLISGVATYLKAKSPITKIIGVEPAGAPSMTEALKQNQVVTLDKIDKF
VDGAAVKEVGSLTFQHAKVLVDEVATVSEGAVCSTILDMYTKQAIVAEPAGALSVAALEKYREEIKDKTVVCIVSGGNND
INRMQEIEERSLLHEGLKHYFIVNFSQRPGALKEFVNDVLGPHDDITKFEYTKKVNRGNGPVIIGVLLQDKNDYEGLLDR
VAAFDPNYIPINDNQTLYTLLV

Sequences:

>Translated_422_residues
MELVDLLVTEEDVEKAYDALKSVVKHTPLEYDFYLSEKYHCNVYLKREDLQRVRSFKLRGAFYAISRLSAAQLEKGVACA
SAGNHAQGVAYTCKRMTVPATIFMPTTTPQQKVSQVRFFGGSNVEVVLVGDTFDASATAAKEYAALHGQTFIPPFDDPDI
IAGQGSLAVEMVKDLNKAHEQADYVFAGIGGGGLISGVATYLKAKSPITKIIGVEPAGAPSMTEALKQNQVVTLDKIDKF
VDGAAVKEVGSLTFQHAKVLVDEVATVSEGAVCSTILDMYTKQAIVAEPAGALSVAALEKYREEIKDKTVVCIVSGGNND
INRMQEIEERSLLHEGLKHYFIVNFSQRPGALKEFVNDVLGPHDDITKFEYTKKVNRGNGPVIIGVLLQDKNDYEGLLDR
VAAFDPNYIPINDNQTLYTLLV
>Mature_422_residues
MELVDLLVTEEDVEKAYDALKSVVKHTPLEYDFYLSEKYHCNVYLKREDLQRVRSFKLRGAFYAISRLSAAQLEKGVACA
SAGNHAQGVAYTCKRMTVPATIFMPTTTPQQKVSQVRFFGGSNVEVVLVGDTFDASATAAKEYAALHGQTFIPPFDDPDI
IAGQGSLAVEMVKDLNKAHEQADYVFAGIGGGGLISGVATYLKAKSPITKIIGVEPAGAPSMTEALKQNQVVTLDKIDKF
VDGAAVKEVGSLTFQHAKVLVDEVATVSEGAVCSTILDMYTKQAIVAEPAGALSVAALEKYREEIKDKTVVCIVSGGNND
INRMQEIEERSLLHEGLKHYFIVNFSQRPGALKEFVNDVLGPHDDITKFEYTKKVNRGNGPVIIGVLLQDKNDYEGLLDR
VAAFDPNYIPINDNQTLYTLLV

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=322, Percent_Identity=30.1242236024845, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI33469958, Length=322, Percent_Identity=29.5031055900621, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI19923959, Length=306, Percent_Identity=27.4509803921569, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI1790207, Length=364, Percent_Identity=37.9120879120879, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1789505, Length=325, Percent_Identity=34.4615384615385, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71991565, Length=353, Percent_Identity=35.6940509915014, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17537387, Length=345, Percent_Identity=30.1449275362319, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17508781, Length=318, Percent_Identity=26.7295597484277, Blast_Score=101, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6320930, Length=361, Percent_Identity=39.0581717451524, Blast_Score=232, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6322631, Length=318, Percent_Identity=34.5911949685535, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6319788, Length=285, Percent_Identity=28.7719298245614, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21355833, Length=349, Percent_Identity=32.9512893982808, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24645328, Length=253, Percent_Identity=31.6205533596838, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 46079; Mature: 46079

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELVDLLVTEEDVEKAYDALKSVVKHTPLEYDFYLSEKYHCNVYLKREDLQRVRSFKLRG
CCHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEEEHHHHHHHHHHHHHH
AFYAISRLSAAQLEKGVACASAGNHAQGVAYTCKRMTVPATIFMPTTTPQQKVSQVRFFG
HHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHCEECCEEEEECCCCHHHHHHHEEEEC
GSNVEVVLVGDTFDASATAAKEYAALHGQTFIPPFDDPDIIAGQGSLAVEMVKDLNKAHE
CCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHH
QADYVFAGIGGGGLISGVATYLKAKSPITKIIGVEPAGAPSMTEALKQNQVVTLDKIDKF
HCCEEEEECCCCHHHHHHHHHHHCCCCHHHHEECCCCCCCHHHHHHHCCCEEEHHHHHHH
VDGAAVKEVGSLTFQHAKVLVDEVATVSEGAVCSTILDMYTKQAIVAEPAGALSVAALEK
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHH
YREEIKDKTVVCIVSGGNNDINRMQEIEERSLLHEGLKHYFIVNFSQRPGALKEFVNDVL
HHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHC
GPHDDITKFEYTKKVNRGNGPVIIGVLLQDKNDYEGLLDRVAAFDPNYIPINDNQTLYTL
CCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCEEEEE
LV
EC
>Mature Secondary Structure
MELVDLLVTEEDVEKAYDALKSVVKHTPLEYDFYLSEKYHCNVYLKREDLQRVRSFKLRG
CCHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEEEHHHHHHHHHHHHHH
AFYAISRLSAAQLEKGVACASAGNHAQGVAYTCKRMTVPATIFMPTTTPQQKVSQVRFFG
HHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHCEECCEEEEECCCCHHHHHHHEEEEC
GSNVEVVLVGDTFDASATAAKEYAALHGQTFIPPFDDPDIIAGQGSLAVEMVKDLNKAHE
CCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHH
QADYVFAGIGGGGLISGVATYLKAKSPITKIIGVEPAGAPSMTEALKQNQVVTLDKIDKF
HCCEEEEECCCCHHHHHHHHHHHCCCCHHHHEECCCCCCCHHHHHHHCCCEEEHHHHHHH
VDGAAVKEVGSLTFQHAKVLVDEVATVSEGAVCSTILDMYTKQAIVAEPAGALSVAALEK
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHH
YREEIKDKTVVCIVSGGNNDINRMQEIEERSLLHEGLKHYFIVNFSQRPGALKEFVNDVL
HHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHC
GPHDDITKFEYTKKVNRGNGPVIIGVLLQDKNDYEGLLDRVAAFDPNYIPINDNQTLYTL
CCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCEEEEE
LV
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA