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The map label for this gene is leuD [H]

Identifier: 116873425

GI number: 116873425

Start: 2037779

End: 2038360

Strand: Direct

Name: leuD [H]

Synonym: lwe2009

Alternate gene names: 116873425

Gene position: 2037779-2038360 (Clockwise)

Preceding gene: 116873424

Following gene: 116873426

Centisome position: 72.41

GC content: 36.43

Gene sequence:

>582_bases
GTGGAGGAAATTAAAGTACACATTGGAAAAACTGTTGCGCTAATGAATGACAACATTGATACCGATCAAATCATACCTAA
AAGTTTTCTAAAACGAATTGAACGAACTGGTTTTGGTGAATTTTTATTTGATAGTTGGCGCTACCTTCCGAATCGTAAAC
CCAATCCAGATTTCCCACTCAATGCCTCGGATCGTCAAGAAGCAACTATTTTAATTACAGGAGATAATTTTGGTTGTGGT
TCTTCTAGAGAGCATGCCGCATGGGCGCTACTTGATTATCGTTTTCGTGTAATTATTGCTGGAAGTTATAGTGATATTTT
TTATATGAATTGTACCAAGAATGGAGTGCTCCCTATCGTTCTTCCTAAAGATGCTCGGGAAAAACTAGCAGAAATCGCTG
CGGATGAGGATGTCACAATTGACTTGCCAAATCAACAAGTCATTAGTTCAGTTGGCACCTATCCTTTTGAAATTGATGCA
ACTTGGAAAAATAAATTTATTAATGGACTGGATGATATCGCTATTACATTTGAACATATTGATGCGATTAAAGCCTATGA
ACAAAAAGTAGATTCCATATAA

Upstream 100 bases:

>100_bases
CAAGGTAAAGGTGCACGTACTCATCTCGTTTCACCAGCAATGGCTGCAGCTGCGGCAATCAACGGACACTTTATTGATAT
TCGAAAGGAGGCGGTTATTA

Downstream 100 bases:

>100_bases
GAAAAGGTAGTGAGGATGATGGAGTTAGTTGATTTATTAGTAACAGAAGAAGATGTCGAAAAAGCCTATGATGCATTAAA
ATCTGTCGTCAAACATACGC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MEEIKVHIGKTVALMNDNIDTDQIIPKSFLKRIERTGFGEFLFDSWRYLPNRKPNPDFPLNASDRQEATILITGDNFGCG
SSREHAAWALLDYRFRVIIAGSYSDIFYMNCTKNGVLPIVLPKDAREKLAEIAADEDVTIDLPNQQVISSVGTYPFEIDA
TWKNKFINGLDDIAITFEHIDAIKAYEQKVDSI

Sequences:

>Translated_193_residues
MEEIKVHIGKTVALMNDNIDTDQIIPKSFLKRIERTGFGEFLFDSWRYLPNRKPNPDFPLNASDRQEATILITGDNFGCG
SSREHAAWALLDYRFRVIIAGSYSDIFYMNCTKNGVLPIVLPKDAREKLAEIAADEDVTIDLPNQQVISSVGTYPFEIDA
TWKNKFINGLDDIAITFEHIDAIKAYEQKVDSI
>Mature_193_residues
MEEIKVHIGKTVALMNDNIDTDQIIPKSFLKRIERTGFGEFLFDSWRYLPNRKPNPDFPLNASDRQEATILITGDNFGCG
SSREHAAWALLDYRFRVIIAGSYSDIFYMNCTKNGVLPIVLPKDAREKLAEIAADEDVTIDLPNQQVISSVGTYPFEIDA
TWKNKFINGLDDIAITFEHIDAIKAYEQKVDSI

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=193, Percent_Identity=48.1865284974093, Blast_Score=176, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6321429, Length=188, Percent_Identity=47.8723404255319, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 21892; Mature: 21892

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEIKVHIGKTVALMNDNIDTDQIIPKSFLKRIERTGFGEFLFDSWRYLPNRKPNPDFPL
CCCEEEEECCEEEEECCCCCCHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCC
NASDRQEATILITGDNFGCGSSREHAAWALLDYRFRVIIAGSYSDIFYMNCTKNGVLPIV
CCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEEEEEEEECCCCEEEEEECCCCEEEEE
LPKDAREKLAEIAADEDVTIDLPNQQVISSVGTYPFEIDATWKNKFINGLDDIAITFEHI
ECCHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCEEEECCHHHHHHCCCHHHEEHHHHH
DAIKAYEQKVDSI
HHHHHHHHHHCCC
>Mature Secondary Structure
MEEIKVHIGKTVALMNDNIDTDQIIPKSFLKRIERTGFGEFLFDSWRYLPNRKPNPDFPL
CCCEEEEECCEEEEECCCCCCHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCC
NASDRQEATILITGDNFGCGSSREHAAWALLDYRFRVIIAGSYSDIFYMNCTKNGVLPIV
CCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEEEEEEEECCCCEEEEEECCCCEEEEE
LPKDAREKLAEIAADEDVTIDLPNQQVISSVGTYPFEIDATWKNKFINGLDDIAITFEHI
ECCHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCEEEECCHHHHHHCCCHHHEEHHHHH
DAIKAYEQKVDSI
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]