Click here to switch to the map view.

The map label for this gene is leuC [H]

Identifier: 116873424

GI number: 116873424

Start: 2036404

End: 2037792

Strand: Direct

Name: leuC [H]

Synonym: lwe2008

Alternate gene names: 116873424

Gene position: 2036404-2037792 (Clockwise)

Preceding gene: 116873423

Following gene: 116873425

Centisome position: 72.36

GC content: 41.4

Gene sequence:

>1389_bases
ATGGGAAAAACGCTTTTTGATAAACTTTGGAATCGTCATGTCATTTATGGCAAAGAAGGTGAACCGCAATTATTATATGT
TGATCTTCATTTGATTCATGAAGTTACTTCTCCTCAAGCATTTGAAGGACTGAGAATGGAAAATCGGCCACTACGTAGAC
CAGATAAAACATTTGCTACAATGGATCATAATGTTCCAACTGAAGATATTTTCAACATTCAAGACCTTGTTGCTAAAAAG
CAAATTGAGGCCTTGCAAACTAATTGCGCAGAATTTGGTGTAACGCTAGCAGATATGGGCAGTGATCGTCAAGGTATTGT
TCACATGGTTGGACCTGAAACCGGACTAACTCAGCCTGGTAAAGTTATTGTTTGTGGGGATTCTCATACAGCGACTCATG
GCGCTTTCGGAGCGATTGGTTTCGGGATTGGTTCTAGTGAAGTAGAACATGTTTTTGCCACTCAAACGATTTGGCAACAA
AAGCCAAAATCAATGGGCATCGAAATTAAAGGCAAACTTCCAAAAGGCGTGTATGCAAAAGATATTATCTTACACTTGAT
TGCCACTTATGGGGTTGCATTTGGCACTGGTTATGCAGTGGAATATTACGGTGAAACGATTCAGAATATGTCGATGGAAG
AACGAATGACGATTTGCAACATGGCGATTGAAGGTGGCGCAAAAATGGGAATGATGGCACCAGATGAAACTACTTTTGAA
TATGTTCGCGGTCGTGAATATGCTCCGACTGATATGGAAAAAGCCATTAGCGATTGGAAAACGCTCCAAACTGATCCTGA
TGCGGAATATGATCTTCATATCGAAATGGATGCAAGTACACTAGAACCATATGTTACTTGGGGAACGAACCCCGAAATGG
GTGTCCCTTTTTCTAGAGCATTTCCGGAAATTAAAGATATGAACTATGAGCGCGCTTATGAATATATGGGACTAAAACCC
GGACAAAAAGCAGAACAAATTGAGCTTGGTTATGTATTTATAGGTTCTTGTACCAACGCTCGACTTTCTGATTTAGAAGA
AGCTGCTCGTATTGTTAAAGGAAACAAAGTGAAAAATAATATTCGCGCACTAGTTGTTCCAGGTTCTCGGCAAGTTCGTA
ATGCGGCCGAATCTATAGGGCTGGATAAAATTTTTATAGCGGCTGGATTTGAATGGCGTGAACCTGGCTGTTCGATGTGT
CTAGGAATGAACCCTGACCAAGTACCAGATGGTGTTCACTGCGCATCTACATCGAATCGTAATTTCGAAGGACGTCAAGG
TAAAGGTGCACGTACTCATCTCGTTTCACCAGCAATGGCTGCAGCTGCGGCAATCAACGGACACTTTATTGATATTCGAA
AGGAGGCGGTTATTAGTGGAGGAAATTAA

Upstream 100 bases:

>100_bases
ACTATGAAAGCTGGATTTTTAACCGCTGATCTTGGAGGCAATACTTCTACAACGGATTTTACAAGCGAAGTTCTAAAACA
AATTGAAGGAGGAGAATAAA

Downstream 100 bases:

>100_bases
AGTACACATTGGAAAAACTGTTGCGCTAATGAATGACAACATTGATACCGATCAAATCATACCTAAAAGTTTTCTAAAAC
GAATTGAACGAACTGGTTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MGKTLFDKLWNRHVIYGKEGEPQLLYVDLHLIHEVTSPQAFEGLRMENRPLRRPDKTFATMDHNVPTEDIFNIQDLVAKK
QIEALQTNCAEFGVTLADMGSDRQGIVHMVGPETGLTQPGKVIVCGDSHTATHGAFGAIGFGIGSSEVEHVFATQTIWQQ
KPKSMGIEIKGKLPKGVYAKDIILHLIATYGVAFGTGYAVEYYGETIQNMSMEERMTICNMAIEGGAKMGMMAPDETTFE
YVRGREYAPTDMEKAISDWKTLQTDPDAEYDLHIEMDASTLEPYVTWGTNPEMGVPFSRAFPEIKDMNYERAYEYMGLKP
GQKAEQIELGYVFIGSCTNARLSDLEEAARIVKGNKVKNNIRALVVPGSRQVRNAAESIGLDKIFIAAGFEWREPGCSMC
LGMNPDQVPDGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAINGHFIDIRKEAVISGGN

Sequences:

>Translated_462_residues
MGKTLFDKLWNRHVIYGKEGEPQLLYVDLHLIHEVTSPQAFEGLRMENRPLRRPDKTFATMDHNVPTEDIFNIQDLVAKK
QIEALQTNCAEFGVTLADMGSDRQGIVHMVGPETGLTQPGKVIVCGDSHTATHGAFGAIGFGIGSSEVEHVFATQTIWQQ
KPKSMGIEIKGKLPKGVYAKDIILHLIATYGVAFGTGYAVEYYGETIQNMSMEERMTICNMAIEGGAKMGMMAPDETTFE
YVRGREYAPTDMEKAISDWKTLQTDPDAEYDLHIEMDASTLEPYVTWGTNPEMGVPFSRAFPEIKDMNYERAYEYMGLKP
GQKAEQIELGYVFIGSCTNARLSDLEEAARIVKGNKVKNNIRALVVPGSRQVRNAAESIGLDKIFIAAGFEWREPGCSMC
LGMNPDQVPDGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAINGHFIDIRKEAVISGGN
>Mature_461_residues
GKTLFDKLWNRHVIYGKEGEPQLLYVDLHLIHEVTSPQAFEGLRMENRPLRRPDKTFATMDHNVPTEDIFNIQDLVAKKQ
IEALQTNCAEFGVTLADMGSDRQGIVHMVGPETGLTQPGKVIVCGDSHTATHGAFGAIGFGIGSSEVEHVFATQTIWQQK
PKSMGIEIKGKLPKGVYAKDIILHLIATYGVAFGTGYAVEYYGETIQNMSMEERMTICNMAIEGGAKMGMMAPDETTFEY
VRGREYAPTDMEKAISDWKTLQTDPDAEYDLHIEMDASTLEPYVTWGTNPEMGVPFSRAFPEIKDMNYERAYEYMGLKPG
QKAEQIELGYVFIGSCTNARLSDLEEAARIVKGNKVKNNIRALVVPGSRQVRNAAESIGLDKIFIAAGFEWREPGCSMCL
GMNPDQVPDGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAINGHFIDIRKEAVISGGN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=379, Percent_Identity=29.023746701847, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI41352693, Length=382, Percent_Identity=27.2251308900524, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI8659555, Length=408, Percent_Identity=26.7156862745098, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=58.1896551724138, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI1787531, Length=375, Percent_Identity=25.6, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081781, Length=360, Percent_Identity=24.1666666666667, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI2367097, Length=344, Percent_Identity=26.453488372093, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI32564738, Length=394, Percent_Identity=30.9644670050761, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25149337, Length=394, Percent_Identity=30.9644670050761, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=304, Percent_Identity=31.5789473684211, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=450, Percent_Identity=26.6666666666667, Blast_Score=100, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=469, Percent_Identity=53.7313432835821, Blast_Score=494, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6320440, Length=412, Percent_Identity=26.2135922330097, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6322261, Length=376, Percent_Identity=30.5851063829787, Blast_Score=146, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6323335, Length=379, Percent_Identity=29.8153034300792, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI281365315, Length=391, Percent_Identity=29.6675191815857, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17864292, Length=391, Percent_Identity=29.6675191815857, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI161076999, Length=391, Percent_Identity=29.6675191815857, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI28571643, Length=391, Percent_Identity=28.1329923273657, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17137564, Length=375, Percent_Identity=26.9333333333333, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24645686, Length=462, Percent_Identity=26.6233766233766, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50914; Mature: 50782

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTLFDKLWNRHVIYGKEGEPQLLYVDLHLIHEVTSPQAFEGLRMENRPLRRPDKTFAT
CCCHHHHHHCCCEEEECCCCCCCEEEEEEEHHHHHCCCHHHHCCCCCCCCCCCCCCHHEE
MDHNVPTEDIFNIQDLVAKKQIEALQTNCAEFGVTLADMGSDRQGIVHMVGPETGLTQPG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCC
KVIVCGDSHTATHGAFGAIGFGIGSSEVEHVFATQTIWQQKPKSMGIEIKGKLPKGVYAK
EEEEECCCCCCCCCCCHHEEECCCCHHHHHHHHHHHHHHCCHHHCCEEEECCCCCCCHHH
DIILHLIATYGVAFGTGYAVEYYGETIQNMSMEERMTICNMAIEGGAKMGMMAPDETTFE
HHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
YVRGREYAPTDMEKAISDWKTLQTDPDAEYDLHIEMDASTLEPYVTWGTNPEMGVPFSRA
HHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCHHHH
FPEIKDMNYERAYEYMGLKPGQKAEQIELGYVFIGSCTNARLSDLEEAARIVKGNKVKNN
CCHHHCCCHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
IRALVVPGSRQVRNAAESIGLDKIFIAAGFEWREPGCSMCLGMNPDQVPDGVHCASTSNR
CEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCEEECCCCCC
NFEGRQGKGARTHLVSPAMAAAAAINGHFIDIRKEAVISGGN
CCCCCCCCCCCCEECCHHHHHHHHHCCEEEEEEHEEEECCCC
>Mature Secondary Structure 
GKTLFDKLWNRHVIYGKEGEPQLLYVDLHLIHEVTSPQAFEGLRMENRPLRRPDKTFAT
CCHHHHHHCCCEEEECCCCCCCEEEEEEEHHHHHCCCHHHHCCCCCCCCCCCCCCHHEE
MDHNVPTEDIFNIQDLVAKKQIEALQTNCAEFGVTLADMGSDRQGIVHMVGPETGLTQPG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCC
KVIVCGDSHTATHGAFGAIGFGIGSSEVEHVFATQTIWQQKPKSMGIEIKGKLPKGVYAK
EEEEECCCCCCCCCCCHHEEECCCCHHHHHHHHHHHHHHCCHHHCCEEEECCCCCCCHHH
DIILHLIATYGVAFGTGYAVEYYGETIQNMSMEERMTICNMAIEGGAKMGMMAPDETTFE
HHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
YVRGREYAPTDMEKAISDWKTLQTDPDAEYDLHIEMDASTLEPYVTWGTNPEMGVPFSRA
HHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCHHHH
FPEIKDMNYERAYEYMGLKPGQKAEQIELGYVFIGSCTNARLSDLEEAARIVKGNKVKNN
CCHHHCCCHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
IRALVVPGSRQVRNAAESIGLDKIFIAAGFEWREPGCSMCLGMNPDQVPDGVHCASTSNR
CEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCEEECCCCCC
NFEGRQGKGARTHLVSPAMAAAAAINGHFIDIRKEAVISGGN
CCCCCCCCCCCCEECCHHHHHHHHHCCEEEEEEHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]