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The map label for this gene is leuA [H]

Identifier: 116873422

GI number: 116873422

Start: 2033810

End: 2035348

Strand: Direct

Name: leuA [H]

Synonym: lwe2006

Alternate gene names: 116873422

Gene position: 2033810-2035348 (Clockwise)

Preceding gene: 116873421

Following gene: 116873423

Centisome position: 72.27

GC content: 40.74

Gene sequence:

>1539_bases
ATGAAGAAAATCCAGTTTTTTGATACTACACTAAGAGATGGCGAACAAACCCCTGGAGTTAATTTTGACGTAAAGGAGAA
AATCCAGATTGCCTTACAACTCGAAAAACTTGGAATTGATGTGATTGAAGCCGGCTTTCCGATTTCTTCTCCTGGAGATT
TTGAATGTGTCAGGGCCATTGCTAAAGCGATTAAACATTGTTCTGTAACAGGGTTAGCGCGCTGCGTTGAAGGAGATATC
GACCGTGCAGAAGAAGCGCTTAAAGAAGCTGTTTCTCCGCAAATACATATCTTCCTAGCAACAAGCGATGTGCACATGGA
ATACAAATTAAAAATGAGTCGGGCGGAAGTACTTGCTTCCATTAAACACCACATCCATTACGCAAGACAAAAATTTGATA
TCGTACAGTTTTCACCAGAAGATGCAACTCGGTCAGATCGAGCTTTTCTGATTGAAGCAGTTCAAACAGCGATTGATGCA
GGCGCGACTGTCATCAACATTCCAGATACGGTCGGGTATACAAATCCAACCGAATTCGGGCAATTATTCCAAGATTTGCG
CCGTGAAATAAAGCAATTTGATGATATTATTTTTGCTTCTCATTGTCATGATGATCTTGGCATGGCGACCGCTAATGCAC
TAGCAGCAATTGAAAACGGTGCAAGACGCGTCGAAGGTACAATTAATGGAATTGGTGAACGTGCCGGTAATACAGCCCTA
GAGGAAGTTGCCGTAGCCCTTCACATCCGCAAAGATTTTTATCAAGCGGAAACCAATATCGTCTTAAATCAATTTAAAAA
CTCAAGTGATTTGATTAGTCGTTTGTCTGGTATGCCAGTTCCTCGTAACAAGGCAGTTATCGGCGGAAATGCTTATGCGC
ATGAATCTGGTATTCATCAAGATGGCGTGCTAAAAAATCCTGATACGTACGAAATCATCACTCCCGCACTAGTCGGCGTA
GATAAAAATTCCCTTCCACTTGGTAAGCTTTCTGGAAAACATGCCTTCCACACACGAATGGAAGAAATGGGCTATACGTT
AAGCGAACAAGAACTTAAAGATACTTTTAAACGTTTTAAACAACTCGCGGATGCTAAAAAAGAAGTAACGGAAGAAGATT
TGCATGCGCTCATTCTTGGGCAGTCTTCTGAATCCACTGATAATTTCGAATTAAAACATCTACAAGTACAATATGTTTCT
GGCGGTGTACAAGGTGCCATCGTTCGTATCGAAGAACGTGATGGCGCTTTGATAGAAGATGCTGCAACAGGTTCTGGTAG
TATTGAAGCCATTTATAACACGATTAATCGTCTAATGAAACAGGATATTGAATTAATTGATTATCGCATCCAAGCGATTA
CGGCTGGCCAAGACGCTCAAGCAGAAGTACATGTCGTTATTAAAGATAATAATGAAACTGAATTCCACGGTATCGGCATC
GACTTTGATGTTTTAACGGCTAGTGCTAAAGCTTATTTACAAGCTTCAGGAAAAAGTAAAAGCACTACAAAGCAAGTTGA
TTTTGAGGAGGTAAAATAA

Upstream 100 bases:

>100_bases
ATATGAGTTTAAGGAAGAACCTATTTCTTTCCTAAACTGATACTGAAAGCAGGAAAAATCCAAGCTTCCCTATCTAATAT
AAAACAAGAGGTGAAATCGA

Downstream 100 bases:

>100_bases
CGTGACATATAAAATTACTTCCTTAGCTGGCGACGGAATTGGTCCAGAAATCATGTCTGCTGGAATCCAAGTATTGGAGT
TAATTGCAAAAAAATACCAC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MKKIQFFDTTLRDGEQTPGVNFDVKEKIQIALQLEKLGIDVIEAGFPISSPGDFECVRAIAKAIKHCSVTGLARCVEGDI
DRAEEALKEAVSPQIHIFLATSDVHMEYKLKMSRAEVLASIKHHIHYARQKFDIVQFSPEDATRSDRAFLIEAVQTAIDA
GATVINIPDTVGYTNPTEFGQLFQDLRREIKQFDDIIFASHCHDDLGMATANALAAIENGARRVEGTINGIGERAGNTAL
EEVAVALHIRKDFYQAETNIVLNQFKNSSDLISRLSGMPVPRNKAVIGGNAYAHESGIHQDGVLKNPDTYEIITPALVGV
DKNSLPLGKLSGKHAFHTRMEEMGYTLSEQELKDTFKRFKQLADAKKEVTEEDLHALILGQSSESTDNFELKHLQVQYVS
GGVQGAIVRIEERDGALIEDAATGSGSIEAIYNTINRLMKQDIELIDYRIQAITAGQDAQAEVHVVIKDNNETEFHGIGI
DFDVLTASAKAYLQASGKSKSTTKQVDFEEVK

Sequences:

>Translated_512_residues
MKKIQFFDTTLRDGEQTPGVNFDVKEKIQIALQLEKLGIDVIEAGFPISSPGDFECVRAIAKAIKHCSVTGLARCVEGDI
DRAEEALKEAVSPQIHIFLATSDVHMEYKLKMSRAEVLASIKHHIHYARQKFDIVQFSPEDATRSDRAFLIEAVQTAIDA
GATVINIPDTVGYTNPTEFGQLFQDLRREIKQFDDIIFASHCHDDLGMATANALAAIENGARRVEGTINGIGERAGNTAL
EEVAVALHIRKDFYQAETNIVLNQFKNSSDLISRLSGMPVPRNKAVIGGNAYAHESGIHQDGVLKNPDTYEIITPALVGV
DKNSLPLGKLSGKHAFHTRMEEMGYTLSEQELKDTFKRFKQLADAKKEVTEEDLHALILGQSSESTDNFELKHLQVQYVS
GGVQGAIVRIEERDGALIEDAATGSGSIEAIYNTINRLMKQDIELIDYRIQAITAGQDAQAEVHVVIKDNNETEFHGIGI
DFDVLTASAKAYLQASGKSKSTTKQVDFEEVK
>Mature_512_residues
MKKIQFFDTTLRDGEQTPGVNFDVKEKIQIALQLEKLGIDVIEAGFPISSPGDFECVRAIAKAIKHCSVTGLARCVEGDI
DRAEEALKEAVSPQIHIFLATSDVHMEYKLKMSRAEVLASIKHHIHYARQKFDIVQFSPEDATRSDRAFLIEAVQTAIDA
GATVINIPDTVGYTNPTEFGQLFQDLRREIKQFDDIIFASHCHDDLGMATANALAAIENGARRVEGTINGIGERAGNTAL
EEVAVALHIRKDFYQAETNIVLNQFKNSSDLISRLSGMPVPRNKAVIGGNAYAHESGIHQDGVLKNPDTYEIITPALVGV
DKNSLPLGKLSGKHAFHTRMEEMGYTLSEQELKDTFKRFKQLADAKKEVTEEDLHALILGQSSESTDNFELKHLQVQYVS
GGVQGAIVRIEERDGALIEDAATGSGSIEAIYNTINRLMKQDIELIDYRIQAITAGQDAQAEVHVVIKDNNETEFHGIGI
DFDVLTASAKAYLQASGKSKSTTKQVDFEEVK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=494, Percent_Identity=45.9514170040486, Blast_Score=434, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6320071, Length=366, Percent_Identity=29.5081967213115, Blast_Score=163, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6320019, Length=366, Percent_Identity=29.2349726775956, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6324682, Length=541, Percent_Identity=25.3234750462107, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6324225, Length=531, Percent_Identity=24.6704331450094, Blast_Score=120, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24582381, Length=265, Percent_Identity=25.2830188679245, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56412; Mature: 56412

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIQFFDTTLRDGEQTPGVNFDVKEKIQIALQLEKLGIDVIEAGFPISSPGDFECVRAI
CCCEEEECHHHCCCCCCCCCCCCHHHHHHEEEEHHHHCCEEEECCCCCCCCCCHHHHHHH
AKAIKHCSVTGLARCVEGDIDRAEEALKEAVSPQIHIFLATSDVHMEYKLKMSRAEVLAS
HHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEEECCEEEEEEEHHHHHHHHHH
IKHHIHYARQKFDIVQFSPEDATRSDRAFLIEAVQTAIDAGATVINIPDTVGYTNPTEFG
HHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHH
QLFQDLRREIKQFDDIIFASHCHDDLGMATANALAAIENGARRVEGTINGIGERAGNTAL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHH
EEVAVALHIRKDFYQAETNIVLNQFKNSSDLISRLSGMPVPRNKAVIGGNAYAHESGIHQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCC
DGVLKNPDTYEIITPALVGVDKNSLPLGKLSGKHAFHTRMEEMGYTLSEQELKDTFKRFK
CCCCCCCCCCEEECHHHEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
QLADAKKEVTEEDLHALILGQSSESTDNFELKHLQVQYVSGGVQGAIVRIEERDGALIED
HHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEEEEEECCCCCEEEEEEECCCCCEEEC
AATGSGSIEAIYNTINRLMKQDIELIDYRIQAITAGQDAQAEVHVVIKDNNETEFHGIGI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEEECCCCCEEEECCC
DFDVLTASAKAYLQASGKSKSTTKQVDFEEVK
CHHHHHHHHHHHHEECCCCCCCCCCCCHHHCC
>Mature Secondary Structure
MKKIQFFDTTLRDGEQTPGVNFDVKEKIQIALQLEKLGIDVIEAGFPISSPGDFECVRAI
CCCEEEECHHHCCCCCCCCCCCCHHHHHHEEEEHHHHCCEEEECCCCCCCCCCHHHHHHH
AKAIKHCSVTGLARCVEGDIDRAEEALKEAVSPQIHIFLATSDVHMEYKLKMSRAEVLAS
HHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEEECCEEEEEEEHHHHHHHHHH
IKHHIHYARQKFDIVQFSPEDATRSDRAFLIEAVQTAIDAGATVINIPDTVGYTNPTEFG
HHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHH
QLFQDLRREIKQFDDIIFASHCHDDLGMATANALAAIENGARRVEGTINGIGERAGNTAL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHH
EEVAVALHIRKDFYQAETNIVLNQFKNSSDLISRLSGMPVPRNKAVIGGNAYAHESGIHQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCC
DGVLKNPDTYEIITPALVGVDKNSLPLGKLSGKHAFHTRMEEMGYTLSEQELKDTFKRFK
CCCCCCCCCCEEECHHHEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
QLADAKKEVTEEDLHALILGQSSESTDNFELKHLQVQYVSGGVQGAIVRIEERDGALIED
HHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEEEEEECCCCCEEEEEEECCCCCEEEC
AATGSGSIEAIYNTINRLMKQDIELIDYRIQAITAGQDAQAEVHVVIKDNNETEFHGIGI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEEECCCCCEEEECCC
DFDVLTASAKAYLQASGKSKSTTKQVDFEEVK
CHHHHHHHHHHHHEECCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]