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The map label for this gene is rnhA [H]

Identifier: 116873315

GI number: 116873315

Start: 1929419

End: 1929820

Strand: Direct

Name: rnhA [H]

Synonym: lwe1899

Alternate gene names: 116873315

Gene position: 1929419-1929820 (Clockwise)

Preceding gene: 116873314

Following gene: 116873326

Centisome position: 68.56

GC content: 38.56

Gene sequence:

>402_bases
ATGGAAGTATTTGTTGATGGAGCAAGTGCTGGAAATCCTGGTCCAAGTGGTGCTGGAATTGTTTTAAAAGCAGAAGGTAT
TTATGAACAATTTGCCGTTCCACTTGCTGTTATGACAAATCATGAAGCCGAATTTATCGCTATTAAACTCGGACTAGAGG
AAGCATTAAAAACACAAGCAACGTTCATTCGTTTGTACTCTGATTCGAAAGTTGCCATTGAAGCTATTCACAAACGGCAC
GCTAAAAACCCTTTATTCAAGCCTCATTTAGAAGCTATTTTAGAAATGGCAGATTCTGTAGAATTGTTTTTTGCTGAGTG
GCGCAATGTAAGTCAAAATAAACAAGCCGACCAACTTGCGCGTCAAGCTATCAAAAAACAGAAGCAAACTGGCATCAATT
AA

Upstream 100 bases:

>100_bases
ACTTAAAACCGGTATGTATTTGCCGGTTTTTTTATTGCAAAAAAAGAGCTTTCCTTCTATAATAAAGCCACATCTACTCA
CACTTTAGGAGGGACATAAT

Downstream 100 bases:

>100_bases
CCAGATTGCTTCTGTTTTTATTGGATATCGCGCGTCAATGTTTTTAATCCGTATTTTTCTACTACGCTAAAAGCATCTTC
ACGAAAACCGTCGTATTTCA

Product: RNase H

Products: 5'-phosphomonoester [C]

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MEVFVDGASAGNPGPSGAGIVLKAEGIYEQFAVPLAVMTNHEAEFIAIKLGLEEALKTQATFIRLYSDSKVAIEAIHKRH
AKNPLFKPHLEAILEMADSVELFFAEWRNVSQNKQADQLARQAIKKQKQTGIN

Sequences:

>Translated_133_residues
MEVFVDGASAGNPGPSGAGIVLKAEGIYEQFAVPLAVMTNHEAEFIAIKLGLEEALKTQATFIRLYSDSKVAIEAIHKRH
AKNPLFKPHLEAILEMADSVELFFAEWRNVSQNKQADQLARQAIKKQKQTGIN
>Mature_133_residues
MEVFVDGASAGNPGPSGAGIVLKAEGIYEQFAVPLAVMTNHEAEFIAIKLGLEEALKTQATFIRLYSDSKVAIEAIHKRH
AKNPLFKPHLEAILEMADSVELFFAEWRNVSQNKQADQLARQAIKKQKQTGIN

Specific function: Not known; does not have RNase H activity [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: 3.1.26.4 [C]

Molecular weight: Translated: 14646; Mature: 14646

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVFVDGASAGNPGPSGAGIVLKAEGIYEQFAVPLAVMTNHEAEFIAIKLGLEEALKTQA
CEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHHHEEECCCCEEEEEEECHHHHHHHHH
TFIRLYSDSKVAIEAIHKRHAKNPLFKPHLEAILEMADSVELFFAEWRNVSQNKQADQLA
HHEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RQAIKKQKQTGIN
HHHHHHHHHCCCC
>Mature Secondary Structure
MEVFVDGASAGNPGPSGAGIVLKAEGIYEQFAVPLAVMTNHEAEFIAIKLGLEEALKTQA
CEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHHHEEECCCCEEEEEEECHHHHHHHHH
TFIRLYSDSKVAIEAIHKRHAKNPLFKPHLEAILEMADSVELFFAEWRNVSQNKQADQLA
HHEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RQAIKKQKQTGIN
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Sulfhydryl reagents [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RNA-DNA hybrid; H2O [C]

Specific reaction: RNA-DNA hybrid + H2O = 5'-phosphomonoester [C]

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: (NH4)2SO4; Dextran [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 9888800 [H]