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The map label for this gene is adhE [H]

Identifier: 116873066

GI number: 116873066

Start: 1656230

End: 1658830

Strand: Direct

Name: adhE [H]

Synonym: lwe1650

Alternate gene names: 116873066

Gene position: 1656230-1658830 (Clockwise)

Preceding gene: 116873054

Following gene: 116873067

Centisome position: 58.85

GC content: 39.68

Gene sequence:

>2601_bases
ATGGCAATTAAAGAAAATGCGGCCCAAGAAGTATTAGAAGTTCAAAAAGTGATTAACAGATTAGCAGACAATGGACAACA
AGCATTGAAAGCATTTGAGAATTACGACCAAGAACAAGTAGATAATATCGTACATGCAATGGCGCTTGCCGGACTCGACC
AACATATGCCCCTTGCAAAATTAGCAGTAGAAGAAACAGGACGAGGATTATACGAAGATAAATGTATTAAAAATATCTTT
GCAACAGAATATATTTGGAACAACATTAAAAACAACAAGACAGTCGGCGTTATTCACGAAGACGTTCAAACTGGTGTTAT
CGAAATTGCAGAACCAGTTGGTGTTGTTGCCGGGGTTACACCAGTAACTAACCCAACATCTACTACACTTTTCAAAGCAA
TTATCGCAATTAAAACACGAAACCCAATCATCTTCGCTTTCCATCCAAGTGCACAACGTTGTTCAGCAGCAGCAGCAAAA
GTTGTTTATGATGCGGCAGTTGCAGCTGGAGCGCCAGAACATTGTATTCAATGGGTAGAAAAACCTTCCCTAGAAGCAAC
CAAACAATTAATGAATCACGATAAAGTAGCACTTGTACTTGCAACTGGTGGTGCTGGAATGGTTAAATCAGCATACTCTA
CTGGTAAACCGGCACTAGGTGTTGGCCCGGGTAACGTACCAGCTTACATTGACAAAACAGCTAAAATCAAACGTTCTGTT
AATGACATCATTCTTTCTAAATCTTTTGACCAAGGAATGATTTGTGCTTCTGAACAAGCAGTTATCGTGGACAAAGAAGT
CGCTAAAGAAGTAAAAGCAGAAATGGAAGCCAATAAATGCTACTTCGTTAAAGGCGCTGAATTTAAAAAATTAGAAAGCT
ATGTAATCAACCCTGAAAAAGGAACACTTAACCCAGATGTAGTTGGTAAATCCCCTGCATGGATTGCAAACCAAGCTGGC
TTCAAAGTTCCTGAAGACACAAAAATTCTTGTGGCTGAAATTAAAGGTGTTGGCGATAAATACCCACTATCTCATGAAAA
ATTAAGCCCAGTTCTTGCATTCATCGAAGCAGCTAACCAAGCAGAAGCATTCGATCGTTGTGAAGAAATGCTCGTATACG
GAGGACTTGGACACTCTGCAGTTATTCACTCTACAGATAAAGAAGTTCAAAAAGCATTCGGTATCCGTATGAAAGCTTGC
CGTATCATTGTAAATGCACCAAGCGCTCAAGGTGGTATTGGTGACATCTATAACGGCTTCATCCCTTCCCTAACACTTGG
TTGTGGATCTTATGGTAAAAACTCTGTATCACAAAATGTAAGTGCGACTAACTTGCTGAACGTTAAACGTATCGCGGATC
GGAGAAATAATATGCAATGGTTCAAACTTCCACCAAAAATTTTCTTTGAAAAATATTCCACTCAATACCTTCAAAAAATG
GAAGGCGTTGAACGCGTATTTATCGTAACTGACCCAGGTATGGTTCAATTCAAATACGTTGATGTAGTAATCGAACACCT
GAAAAAACGCGGTAACGACGTAGCTTACCAAGTATTCGCTGATGTTGAACCAGATCCATCTGACGTTACAGTTTATAAAG
GTGCAGAATTAATGAAAGACTTTAAACCAGATACAATCATCGCTCTTGGTGGTGGTTCTGCAATGGATGCTGCTAAAGGT
ATGTGGTTATTCTATGAACACCCAGAAGCTTCATTCTTTGGCTTGAAACAAAAATTCTTAGATATCCGCAAACGTACTTT
CAAATATCCTAAACTTGGTGGAAAAGCGAAATTCGTTGCAATTCCAACAACAAGTGGTACTGGTTCTGAAGTAACTCCAT
TTGCGGTTATTACAGACAAAGAAAATAACATTAAATACCCTCTTGCTGACTACGAATTAACTCCAGACGTTGCGATTGTC
GATGCACAATATGTAACTACTGTTCCAGCACACATTACTGCTGATACTGGTATGGACGTTCTAACTCATGCAATTGAATC
TTATGTATCCGTAATGGCAAGCGATTATACTCGTGGATTATCCATCCGTGCAATCGAACTTGTTTTTGAAAACTTACGTG
AATCCGTTCTTACTGGTGATCCAGATGCACGTGAAAAAATGCATAATGCTTCTGCCCTAGCTGGTATGGCATTTGCGAAT
GCGTTCCTAGGAATTAACCATAGCTTGGCACACAAAATTGGACCTGAATTCCACATTCCTCACGGTCGTGCGAATGCAAT
CCTTATGCCACATGTTATCCGCTATAACGCACTTAAACCTAAAAAACATGCGTTATTCCCAAGATATGAAAGCTTCCGTG
CCGATGAAGATTATGCTCGTATTTCTCGTATTATCGGTTTACCAGCTGCAACTACAGAAGAAGGCGTTAAATCTCTTGTA
GATGCAATTATCAAACTTGGTAAAGATGTTGGTATCGACATGAGTCTTAAAGGACAAAATGTTGCTAAAAAAGACTTGGA
TGCTGTAGTAGATACGCTTGCTGATCGCGCATTCATGGACCAATGTACTACTGCCAACCCTAAACAACCACTTGTAAGCG
AATTGAAAGAAATCTATCTTGAAGCTTACAAAGGGGTTTGA

Upstream 100 bases:

>100_bases
ACGGCAAAGCAACAAGCTAAATTTTTTTGGATTATCAGGGACGTCAAAAGTCTACTATGGACGTTTAGAGTCCCGTACAA
ATATTAGGAGGTTCTGGAAA

Downstream 100 bases:

>100_bases
AACTAGCTAACTTGTCCACTATTAATATATAAAGTTAGAAGAAAGCGCAAGTCCATCCCAGGGCTTGCGCTTTCGCTTTA
GTGTCTAAATTAGTATAATA

Product: bifunctional acetaldehyde-CoA/alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 866; Mature: 865

Protein sequence:

>866_residues
MAIKENAAQEVLEVQKVINRLADNGQQALKAFENYDQEQVDNIVHAMALAGLDQHMPLAKLAVEETGRGLYEDKCIKNIF
ATEYIWNNIKNNKTVGVIHEDVQTGVIEIAEPVGVVAGVTPVTNPTSTTLFKAIIAIKTRNPIIFAFHPSAQRCSAAAAK
VVYDAAVAAGAPEHCIQWVEKPSLEATKQLMNHDKVALVLATGGAGMVKSAYSTGKPALGVGPGNVPAYIDKTAKIKRSV
NDIILSKSFDQGMICASEQAVIVDKEVAKEVKAEMEANKCYFVKGAEFKKLESYVINPEKGTLNPDVVGKSPAWIANQAG
FKVPEDTKILVAEIKGVGDKYPLSHEKLSPVLAFIEAANQAEAFDRCEEMLVYGGLGHSAVIHSTDKEVQKAFGIRMKAC
RIIVNAPSAQGGIGDIYNGFIPSLTLGCGSYGKNSVSQNVSATNLLNVKRIADRRNNMQWFKLPPKIFFEKYSTQYLQKM
EGVERVFIVTDPGMVQFKYVDVVIEHLKKRGNDVAYQVFADVEPDPSDVTVYKGAELMKDFKPDTIIALGGGSAMDAAKG
MWLFYEHPEASFFGLKQKFLDIRKRTFKYPKLGGKAKFVAIPTTSGTGSEVTPFAVITDKENNIKYPLADYELTPDVAIV
DAQYVTTVPAHITADTGMDVLTHAIESYVSVMASDYTRGLSIRAIELVFENLRESVLTGDPDAREKMHNASALAGMAFAN
AFLGINHSLAHKIGPEFHIPHGRANAILMPHVIRYNALKPKKHALFPRYESFRADEDYARISRIIGLPAATTEEGVKSLV
DAIIKLGKDVGIDMSLKGQNVAKKDLDAVVDTLADRAFMDQCTTANPKQPLVSELKEIYLEAYKGV

Sequences:

>Translated_866_residues
MAIKENAAQEVLEVQKVINRLADNGQQALKAFENYDQEQVDNIVHAMALAGLDQHMPLAKLAVEETGRGLYEDKCIKNIF
ATEYIWNNIKNNKTVGVIHEDVQTGVIEIAEPVGVVAGVTPVTNPTSTTLFKAIIAIKTRNPIIFAFHPSAQRCSAAAAK
VVYDAAVAAGAPEHCIQWVEKPSLEATKQLMNHDKVALVLATGGAGMVKSAYSTGKPALGVGPGNVPAYIDKTAKIKRSV
NDIILSKSFDQGMICASEQAVIVDKEVAKEVKAEMEANKCYFVKGAEFKKLESYVINPEKGTLNPDVVGKSPAWIANQAG
FKVPEDTKILVAEIKGVGDKYPLSHEKLSPVLAFIEAANQAEAFDRCEEMLVYGGLGHSAVIHSTDKEVQKAFGIRMKAC
RIIVNAPSAQGGIGDIYNGFIPSLTLGCGSYGKNSVSQNVSATNLLNVKRIADRRNNMQWFKLPPKIFFEKYSTQYLQKM
EGVERVFIVTDPGMVQFKYVDVVIEHLKKRGNDVAYQVFADVEPDPSDVTVYKGAELMKDFKPDTIIALGGGSAMDAAKG
MWLFYEHPEASFFGLKQKFLDIRKRTFKYPKLGGKAKFVAIPTTSGTGSEVTPFAVITDKENNIKYPLADYELTPDVAIV
DAQYVTTVPAHITADTGMDVLTHAIESYVSVMASDYTRGLSIRAIELVFENLRESVLTGDPDAREKMHNASALAGMAFAN
AFLGINHSLAHKIGPEFHIPHGRANAILMPHVIRYNALKPKKHALFPRYESFRADEDYARISRIIGLPAATTEEGVKSLV
DAIIKLGKDVGIDMSLKGQNVAKKDLDAVVDTLADRAFMDQCTTANPKQPLVSELKEIYLEAYKGV
>Mature_865_residues
AIKENAAQEVLEVQKVINRLADNGQQALKAFENYDQEQVDNIVHAMALAGLDQHMPLAKLAVEETGRGLYEDKCIKNIFA
TEYIWNNIKNNKTVGVIHEDVQTGVIEIAEPVGVVAGVTPVTNPTSTTLFKAIIAIKTRNPIIFAFHPSAQRCSAAAAKV
VYDAAVAAGAPEHCIQWVEKPSLEATKQLMNHDKVALVLATGGAGMVKSAYSTGKPALGVGPGNVPAYIDKTAKIKRSVN
DIILSKSFDQGMICASEQAVIVDKEVAKEVKAEMEANKCYFVKGAEFKKLESYVINPEKGTLNPDVVGKSPAWIANQAGF
KVPEDTKILVAEIKGVGDKYPLSHEKLSPVLAFIEAANQAEAFDRCEEMLVYGGLGHSAVIHSTDKEVQKAFGIRMKACR
IIVNAPSAQGGIGDIYNGFIPSLTLGCGSYGKNSVSQNVSATNLLNVKRIADRRNNMQWFKLPPKIFFEKYSTQYLQKME
GVERVFIVTDPGMVQFKYVDVVIEHLKKRGNDVAYQVFADVEPDPSDVTVYKGAELMKDFKPDTIIALGGGSAMDAAKGM
WLFYEHPEASFFGLKQKFLDIRKRTFKYPKLGGKAKFVAIPTTSGTGSEVTPFAVITDKENNIKYPLADYELTPDVAIVD
AQYVTTVPAHITADTGMDVLTHAIESYVSVMASDYTRGLSIRAIELVFENLRESVLTGDPDAREKMHNASALAGMAFANA
FLGINHSLAHKIGPEFHIPHGRANAILMPHVIRYNALKPKKHALFPRYESFRADEDYARISRIIGLPAATTEEGVKSLVD
AIIKLGKDVGIDMSLKGQNVAKKDLDAVVDTLADRAFMDQCTTANPKQPLVSELKEIYLEAYKGV

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=386, Percent_Identity=26.4248704663212, Blast_Score=100, Evalue=8e-21,
Organism=Escherichia coli, GI1787493, Length=861, Percent_Identity=48.780487804878, Blast_Score=797, Evalue=0.0,
Organism=Escherichia coli, GI1789163, Length=387, Percent_Identity=36.1757105943152, Blast_Score=217, Evalue=3e-57,
Organism=Escherichia coli, GI48994951, Length=387, Percent_Identity=34.1085271317829, Blast_Score=195, Evalue=9e-51,
Organism=Escherichia coli, GI87082107, Length=425, Percent_Identity=30.8235294117647, Blast_Score=189, Evalue=9e-49,
Organism=Escherichia coli, GI1788797, Length=383, Percent_Identity=33.1592689295039, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1789386, Length=325, Percent_Identity=25.2307692307692, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17537053, Length=428, Percent_Identity=24.5327102803738, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321181, Length=418, Percent_Identity=31.5789473684211, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24657991, Length=423, Percent_Identity=26.9503546099291, Blast_Score=108, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 94595; Mature: 94463

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKENAAQEVLEVQKVINRLADNGQQALKAFENYDQEQVDNIVHAMALAGLDQHMPLAK
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHH
LAVEETGRGLYEDKCIKNIFATEYIWNNIKNNKTVGVIHEDVQTGVIEIAEPVGVVAGVT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCC
PVTNPTSTTLFKAIIAIKTRNPIIFAFHPSAQRCSAAAAKVVYDAAVAAGAPEHCIQWVE
CCCCCCHHHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
KPSLEATKQLMNHDKVALVLATGGAGMVKSAYSTGKPALGVGPGNVPAYIDKTAKIKRSV
CCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
NDIILSKSFDQGMICASEQAVIVDKEVAKEVKAEMEANKCYFVKGAEFKKLESYVINPEK
HHHHHCCCCCCCCEECCCCEEEECHHHHHHHHHHHCCCCEEEEECCCHHHHHHHEECCCC
GTLNPDVVGKSPAWIANQAGFKVPEDTKILVAEIKGVGDKYPLSHEKLSPVLAFIEAANQ
CCCCCCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
AEAFDRCEEMLVYGGLGHSAVIHSTDKEVQKAFGIRMKACRIIVNAPSAQGGIGDIYNGF
HHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHCCEEEEEEEEEECCCCCCCCHHHHHCH
IPSLTLGCGSYGKNSVSQNVSATNLLNVKRIADRRNNMQWFKLPPKIFFEKYSTQYLQKM
HCHHEECCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHH
EGVERVFIVTDPGMVQFKYVDVVIEHLKKRGNDVAYQVFADVEPDPSDVTVYKGAELMKD
CCCCEEEEEECCCEEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCHHHHHH
FKPDTIIALGGGSAMDAAKGMWLFYEHPEASFFGLKQKFLDIRKRTFKYPKLGGKAKFVA
CCCCEEEEECCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
IPTTSGTGSEVTPFAVITDKENNIKYPLADYELTPDVAIVDAQYVTTVPAHITADTGMDV
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECHHHEECCCEEECCCCHHH
LTHAIESYVSVMASDYTRGLSIRAIELVFENLRESVLTGDPDAREKMHNASALAGMAFAN
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AFLGINHSLAHKIGPEFHIPHGRANAILMPHVIRYNALKPKKHALFPRYESFRADEDYAR
HHHCCCHHHHHHCCCCEECCCCCCCEEECCHHHHHCCCCCCHHCCCCCHHHCCCCHHHHH
ISRIIGLPAATTEEGVKSLVDAIIKLGKDVGIDMSLKGQNVAKKDLDAVVDTLADRAFMD
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
QCTTANPKQPLVSELKEIYLEAYKGV
HHCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIKENAAQEVLEVQKVINRLADNGQQALKAFENYDQEQVDNIVHAMALAGLDQHMPLAK
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHH
LAVEETGRGLYEDKCIKNIFATEYIWNNIKNNKTVGVIHEDVQTGVIEIAEPVGVVAGVT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCC
PVTNPTSTTLFKAIIAIKTRNPIIFAFHPSAQRCSAAAAKVVYDAAVAAGAPEHCIQWVE
CCCCCCHHHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
KPSLEATKQLMNHDKVALVLATGGAGMVKSAYSTGKPALGVGPGNVPAYIDKTAKIKRSV
CCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
NDIILSKSFDQGMICASEQAVIVDKEVAKEVKAEMEANKCYFVKGAEFKKLESYVINPEK
HHHHHCCCCCCCCEECCCCEEEECHHHHHHHHHHHCCCCEEEEECCCHHHHHHHEECCCC
GTLNPDVVGKSPAWIANQAGFKVPEDTKILVAEIKGVGDKYPLSHEKLSPVLAFIEAANQ
CCCCCCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
AEAFDRCEEMLVYGGLGHSAVIHSTDKEVQKAFGIRMKACRIIVNAPSAQGGIGDIYNGF
HHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHCCEEEEEEEEEECCCCCCCCHHHHHCH
IPSLTLGCGSYGKNSVSQNVSATNLLNVKRIADRRNNMQWFKLPPKIFFEKYSTQYLQKM
HCHHEECCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHH
EGVERVFIVTDPGMVQFKYVDVVIEHLKKRGNDVAYQVFADVEPDPSDVTVYKGAELMKD
CCCCEEEEEECCCEEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCHHHHHH
FKPDTIIALGGGSAMDAAKGMWLFYEHPEASFFGLKQKFLDIRKRTFKYPKLGGKAKFVA
CCCCEEEEECCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
IPTTSGTGSEVTPFAVITDKENNIKYPLADYELTPDVAIVDAQYVTTVPAHITADTGMDV
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECHHHEECCCEEECCCCHHH
LTHAIESYVSVMASDYTRGLSIRAIELVFENLRESVLTGDPDAREKMHNASALAGMAFAN
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AFLGINHSLAHKIGPEFHIPHGRANAILMPHVIRYNALKPKKHALFPRYESFRADEDYAR
HHHCCCHHHHHHCCCCEECCCCCCCEEECCHHHHHCCCCCCHHCCCCCHHHCCCCHHHHH
ISRIIGLPAATTEEGVKSLVDAIIKLGKDVGIDMSLKGQNVAKKDLDAVVDTLADRAFMD
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
QCTTANPKQPLVSELKEIYLEAYKGV
HHCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]