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The map label for this gene is nrgA [H]

Identifier: 116872945

GI number: 116872945

Start: 1526106

End: 1527311

Strand: Direct

Name: nrgA [H]

Synonym: lwe1529

Alternate gene names: 116872945

Gene position: 1526106-1527311 (Clockwise)

Preceding gene: 116872944

Following gene: 116872946

Centisome position: 54.23

GC content: 40.13

Gene sequence:

>1206_bases
ATGGAATCAGTATTTATGTACTTTTGTACTTTATTGGTTTGGTTGATGACACCTGGGATTGCATTATTTTATGGCGGTAT
GGTTCGACGCAAAAATGTGTTAAGTACCGCAATGTATAGTTTTAGCTCTATGGCAATTATTTCTATTCTATGGGTAATTG
TTGGATATTCATTAGCTTTTGCACCCGGAAATGGCTTTATTGGCAGTTTTGACTGGACTTTCCTTCATAATGTCGGCTTT
GCGACAAACGATACTTACTCAGATGCAATTCCACATGTTTTATTTATGATGTTCCAAATGACTTTTGCTATTTTGACTGT
AGCTATCATTTCTGGAGCATTTGCAGAGCGGATGAATTTCTCTGCATACTTAATTTTCATTATCTTATGGTCATTACTTG
TTTATTCCCCTGTTGCTCACTGGGTTTGGGGTGATGGTGGTTGGTTACGTAATTTAGGAGCACTTGATTTCGCTGGCGGA
AACGTGGTTCATATTAGTTCTGGTGTCACAGGATTAGTACTTGCGATTATGATAGGTCGAAGAAAAGAAGCGGACTCTGC
TTCACCACATAACTTACCTCTCGCACTTATTGGTGGTATTCTAGTTTGGTTTGGCTGGTATGGATTTAATGTTGGTAGTG
CTTTAACTATTGATAATGTGGCCATGACAGCTTTTGTCAATACTAACACCGCCGCAGCTGCTGGTATTATCGGTTGGGGG
TTAGTAGAATGGCTGATCAATAAAAAACCAACAATGCTCGGTACAATTTCTGGAGCAATCGCTGGTTTAGTTTCTATTAC
TCCCGCTGCTGGCTTTGTTACCGTACCAAGTTCTCTTATTATCGGTTTCCTTGGCGGAGCACTTTGCTTCTGGGCTGTAT
TCTGGTTAAAAGGAAAAGTAAAATACGATGATGCACTAGATGCGTTCGGTCTTCATGGCATTGGAGGTATTTGGGGCGGT
ATCGCAACGGGTCTTTTTGCAACAACTAAAGTAAATGAGGCTGGAGCTGACGGTCTCTTTTACGGTAACGCCTCTCTCGT
GGTAAAACAATTGATTGCCATTGGTTCTACTGTCGCATATGTAGCTGTTGCAACGGCTCTCATTGTCGTAATTATTAAGT
TATTTTTACCAATCCGAGTAAATGAAGAACAAGAATATAAAGGACTTGATTTGACGCTACATGGCGAAAAAGCATATCAA
GAATAA

Upstream 100 bases:

>100_bases
GAATTGATAAAATATTCTTGCCTTTATTTAAAATTAGTGTATTATAAACGCATAAATAAAATATTACATGTTAGTTTATC
TAACAAAAGGAGAGTTGAGT

Downstream 100 bases:

>100_bases
GGGGGCAACAATATGTCAGGATTAACGAAAATTGAAATCATCACTCGTCCAAATCGTTTTAATTTATTTCAAAAAGAGCT
TGCAAAAATTGGCGTAAGTG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MESVFMYFCTLLVWLMTPGIALFYGGMVRRKNVLSTAMYSFSSMAIISILWVIVGYSLAFAPGNGFIGSFDWTFLHNVGF
ATNDTYSDAIPHVLFMMFQMTFAILTVAIISGAFAERMNFSAYLIFIILWSLLVYSPVAHWVWGDGGWLRNLGALDFAGG
NVVHISSGVTGLVLAIMIGRRKEADSASPHNLPLALIGGILVWFGWYGFNVGSALTIDNVAMTAFVNTNTAAAAGIIGWG
LVEWLINKKPTMLGTISGAIAGLVSITPAAGFVTVPSSLIIGFLGGALCFWAVFWLKGKVKYDDALDAFGLHGIGGIWGG
IATGLFATTKVNEAGADGLFYGNASLVVKQLIAIGSTVAYVAVATALIVVIIKLFLPIRVNEEQEYKGLDLTLHGEKAYQ
E

Sequences:

>Translated_401_residues
MESVFMYFCTLLVWLMTPGIALFYGGMVRRKNVLSTAMYSFSSMAIISILWVIVGYSLAFAPGNGFIGSFDWTFLHNVGF
ATNDTYSDAIPHVLFMMFQMTFAILTVAIISGAFAERMNFSAYLIFIILWSLLVYSPVAHWVWGDGGWLRNLGALDFAGG
NVVHISSGVTGLVLAIMIGRRKEADSASPHNLPLALIGGILVWFGWYGFNVGSALTIDNVAMTAFVNTNTAAAAGIIGWG
LVEWLINKKPTMLGTISGAIAGLVSITPAAGFVTVPSSLIIGFLGGALCFWAVFWLKGKVKYDDALDAFGLHGIGGIWGG
IATGLFATTKVNEAGADGLFYGNASLVVKQLIAIGSTVAYVAVATALIVVIIKLFLPIRVNEEQEYKGLDLTLHGEKAYQ
E
>Mature_401_residues
MESVFMYFCTLLVWLMTPGIALFYGGMVRRKNVLSTAMYSFSSMAIISILWVIVGYSLAFAPGNGFIGSFDWTFLHNVGF
ATNDTYSDAIPHVLFMMFQMTFAILTVAIISGAFAERMNFSAYLIFIILWSLLVYSPVAHWVWGDGGWLRNLGALDFAGG
NVVHISSGVTGLVLAIMIGRRKEADSASPHNLPLALIGGILVWFGWYGFNVGSALTIDNVAMTAFVNTNTAAAAGIIGWG
LVEWLINKKPTMLGTISGAIAGLVSITPAAGFVTVPSSLIIGFLGGALCFWAVFWLKGKVKYDDALDAFGLHGIGGIWGG
IATGLFATTKVNEAGADGLFYGNASLVVKQLIAIGSTVAYVAVATALIVVIIKLFLPIRVNEEQEYKGLDLTLHGEKAYQ
E

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=405, Percent_Identity=46.9135802469136, Blast_Score=328, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI17550182, Length=438, Percent_Identity=28.9954337899543, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI133931100, Length=420, Percent_Identity=27.3809523809524, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17550180, Length=434, Percent_Identity=27.6497695852535, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32564042, Length=416, Percent_Identity=26.6826923076923, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324187, Length=408, Percent_Identity=37.5, Blast_Score=243, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6325396, Length=409, Percent_Identity=35.9413202933985, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6321559, Length=411, Percent_Identity=35.5231143552311, Blast_Score=226, Evalue=4e-60,
Organism=Drosophila melanogaster, GI161078314, Length=410, Percent_Identity=30.4878048780488, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43023; Mature: 43023

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESVFMYFCTLLVWLMTPGIALFYGGMVRRKNVLSTAMYSFSSMAIISILWVIVGYSLAF
CCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
APGNGFIGSFDWTFLHNVGFATNDTYSDAIPHVLFMMFQMTFAILTVAIISGAFAERMNF
CCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SAYLIFIILWSLLVYSPVAHWVWGDGGWLRNLGALDFAGGNVVHISSGVTGLVLAIMIGR
HHHHHHHHHHHHHHHHHHHHHEECCCHHHHCCCCEEECCCCEEEECCCHHHHHHHHHHCC
RKEADSASPHNLPLALIGGILVWFGWYGFNVGSALTIDNVAMTAFVNTNTAAAAGIIGWG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEEECCCHHHHHHHHHH
LVEWLINKKPTMLGTISGAIAGLVSITPAAGFVTVPSSLIIGFLGGALCFWAVFWLKGKV
HHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCC
KYDDALDAFGLHGIGGIWGGIATGLFATTKVNEAGADGLFYGNASLVVKQLIAIGSTVAY
CCCHHHHHCCCCCHHHHHHHHHHHHHHEEECCCCCCCCEEECCHHHHHHHHHHHCCHHHH
VAVATALIVVIIKLFLPIRVNEEQEYKGLDLTLHGEKAYQE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHCCC
>Mature Secondary Structure
MESVFMYFCTLLVWLMTPGIALFYGGMVRRKNVLSTAMYSFSSMAIISILWVIVGYSLAF
CCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
APGNGFIGSFDWTFLHNVGFATNDTYSDAIPHVLFMMFQMTFAILTVAIISGAFAERMNF
CCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SAYLIFIILWSLLVYSPVAHWVWGDGGWLRNLGALDFAGGNVVHISSGVTGLVLAIMIGR
HHHHHHHHHHHHHHHHHHHHHEECCCHHHHCCCCEEECCCCEEEECCCHHHHHHHHHHCC
RKEADSASPHNLPLALIGGILVWFGWYGFNVGSALTIDNVAMTAFVNTNTAAAAGIIGWG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEEECCCHHHHHHHHHH
LVEWLINKKPTMLGTISGAIAGLVSITPAAGFVTVPSSLIIGFLGGALCFWAVFWLKGKV
HHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCC
KYDDALDAFGLHGIGGIWGGIATGLFATTKVNEAGADGLFYGNASLVVKQLIAIGSTVAY
CCCHHHHHCCCCCHHHHHHHHHHHHHHEEECCCCCCCCEEECCHHHHHHHHHHHCCHHHH
VAVATALIVVIIKLFLPIRVNEEQEYKGLDLTLHGEKAYQE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]