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The map label for this gene is bfmBC [H]

Identifier: 116872802

GI number: 116872802

Start: 1373539

End: 1374966

Strand: Direct

Name: bfmBC [H]

Synonym: lwe1386

Alternate gene names: 116872802

Gene position: 1373539-1374966 (Clockwise)

Preceding gene: 116872801

Following gene: 116872803

Centisome position: 48.81

GC content: 39.57

Gene sequence:

>1428_bases
GTGGCAACAGAATATGATGTAGTTGTTCTTGGCGGAGGAACTGGCGGTTACGTCGCAGCAATCCAAGCAGCTAAGAATGG
CCAAAAAGTAGCCGTAGTAGAAAAAGGTAAAGTTGGCGGGACATGTCTTCACCGAGGATGTATCCCAACTAAAGCTTTGC
TACGTTCTGCTGAGGTTTTACAAACGGTGAAAAAAGCGAGTGAATTTGGTGTTTCTGTAGAAGGAAATGCAGGAATCAAC
TTTTTGCAAGCACAAGAAAGAAAACAACAAATAGTAGATCAATTAGAAAAAGGTATTCATCAATTATTCAAACAAGGCAA
AATTGATTTGTTCGTTGGGACGGGAACTATTCTAGGGCCATCCATCTTTTCTCCAACAGCAGGGACTGTTTCAGTTGAAC
TAGAAGATGGTTCCGAAAATGAAATGCTAATCCCTAAAAATTTAATTATTGCGACTGGTTCGAAACCTCGCACATTAAAC
GGTTTGACCATTGATGAAGAAAATGTTTTATCATCTGATGGTGCGCTCAATCTAGAAACTTTACCAAAATCAATTCTTAT
CGTTGGTGGTGGGGTTATTGGAATGGAGTGGGCTTCGATGTTGCATGATTTTGGGGTTGAAGTTACTGTACTTGAATATG
CGGATCGGATTTTGCCAACAGAAGATAAAGAAGTAGCCAAAGAACTAGCAAGACTCTATAAAAAGAAAAATTTAACAATG
CACACATCTGCTGAAGTTCAAGCAGCTAGCTATAAAAAAACAAATAATGGTGTAGAAATTAAAGCAATTATTAAAGGCGA
AGAGCAGACTTTCTCAGCAGATAAAATTCTTGTATCTGTGGGGCGTTCAGCTAATACCGAAAATATTGGCCTACAAAACA
CGGATATCGCGACTGAAAATGGCTTTATCCAAGTAAATGATTTTTACCAAACAAAAGAAAGTCACATCTATGCGATTGGT
GACTGTATTCCAACAATTCAACTTGCTCATGTAGCGATGGAAGAAGGAACAATTGCCGCCAACCATATTGCTGGAAAAGC
TACTGAAAAACTTGATTATGACTTAGTTCCGCGCTGTATTTATACTTCCACTGAAATCGCAAGTGTCGGTATCACAGAAG
AACAAGCAAAAGAACGCGGTCACGACGTTAAAAAAGGTAAATTCTTCTTCCGTGGTATTGGAAAAGCGCTCGTTTACGGT
GAATCTGATGGTTTTATCAAAATTATTGCAGATAAAAAAACAGATGATATCCTTGGTGTGAGTATGATTGGACCGCATGT
AACGGACATGATTAGCGAAGCCGCTTTAGCACAAGTTTTAAATGCAACCCCGTGGGAAGTAGGTAATACGATTCACCCGC
ACCCAACTTTAGCAGAAAGTTTTAGAGAAGCTGCCCTTGCTGTGGATGGTAACGCGATTCATGGTTAA

Upstream 100 bases:

>100_bases
ATAGAACCTGGAGAAGATGAACTGGAAGCCTTAAATAGCGGTGTACAACGTGTGCTCGCTGGACTTGAAAAAGAAAAATT
GTACTAGAGGAGGAGATAAT

Downstream 100 bases:

>100_bases
TACAGCTTTAAAGGAGGAACTAAAATGACTTTAAAAGAAGCAGGTTTAACAGAAGATAAATTAATTAAAATGTATGAAAC
AATGTTAATGGCAAGAAGAC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MATEYDVVVLGGGTGGYVAAIQAAKNGQKVAVVEKGKVGGTCLHRGCIPTKALLRSAEVLQTVKKASEFGVSVEGNAGIN
FLQAQERKQQIVDQLEKGIHQLFKQGKIDLFVGTGTILGPSIFSPTAGTVSVELEDGSENEMLIPKNLIIATGSKPRTLN
GLTIDEENVLSSDGALNLETLPKSILIVGGGVIGMEWASMLHDFGVEVTVLEYADRILPTEDKEVAKELARLYKKKNLTM
HTSAEVQAASYKKTNNGVEIKAIIKGEEQTFSADKILVSVGRSANTENIGLQNTDIATENGFIQVNDFYQTKESHIYAIG
DCIPTIQLAHVAMEEGTIAANHIAGKATEKLDYDLVPRCIYTSTEIASVGITEEQAKERGHDVKKGKFFFRGIGKALVYG
ESDGFIKIIADKKTDDILGVSMIGPHVTDMISEAALAQVLNATPWEVGNTIHPHPTLAESFREAALAVDGNAIHG

Sequences:

>Translated_475_residues
MATEYDVVVLGGGTGGYVAAIQAAKNGQKVAVVEKGKVGGTCLHRGCIPTKALLRSAEVLQTVKKASEFGVSVEGNAGIN
FLQAQERKQQIVDQLEKGIHQLFKQGKIDLFVGTGTILGPSIFSPTAGTVSVELEDGSENEMLIPKNLIIATGSKPRTLN
GLTIDEENVLSSDGALNLETLPKSILIVGGGVIGMEWASMLHDFGVEVTVLEYADRILPTEDKEVAKELARLYKKKNLTM
HTSAEVQAASYKKTNNGVEIKAIIKGEEQTFSADKILVSVGRSANTENIGLQNTDIATENGFIQVNDFYQTKESHIYAIG
DCIPTIQLAHVAMEEGTIAANHIAGKATEKLDYDLVPRCIYTSTEIASVGITEEQAKERGHDVKKGKFFFRGIGKALVYG
ESDGFIKIIADKKTDDILGVSMIGPHVTDMISEAALAQVLNATPWEVGNTIHPHPTLAESFREAALAVDGNAIHG
>Mature_474_residues
ATEYDVVVLGGGTGGYVAAIQAAKNGQKVAVVEKGKVGGTCLHRGCIPTKALLRSAEVLQTVKKASEFGVSVEGNAGINF
LQAQERKQQIVDQLEKGIHQLFKQGKIDLFVGTGTILGPSIFSPTAGTVSVELEDGSENEMLIPKNLIIATGSKPRTLNG
LTIDEENVLSSDGALNLETLPKSILIVGGGVIGMEWASMLHDFGVEVTVLEYADRILPTEDKEVAKELARLYKKKNLTMH
TSAEVQAASYKKTNNGVEIKAIIKGEEQTFSADKILVSVGRSANTENIGLQNTDIATENGFIQVNDFYQTKESHIYAIGD
CIPTIQLAHVAMEEGTIAANHIAGKATEKLDYDLVPRCIYTSTEIASVGITEEQAKERGHDVKKGKFFFRGIGKALVYGE
SDGFIKIIADKKTDDILGVSMIGPHVTDMISEAALAQVLNATPWEVGNTIHPHPTLAESFREAALAVDGNAIHG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=468, Percent_Identity=38.4615384615385, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI50301238, Length=469, Percent_Identity=27.7185501066098, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519430, Length=470, Percent_Identity=26.1702127659574, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI33519428, Length=470, Percent_Identity=26.1702127659574, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI33519426, Length=470, Percent_Identity=26.1702127659574, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI148277065, Length=470, Percent_Identity=26.1702127659574, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI148277071, Length=465, Percent_Identity=25.5913978494624, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI291045266, Length=482, Percent_Identity=26.9709543568465, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI22035672, Length=480, Percent_Identity=24.7916666666667, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI291045268, Length=479, Percent_Identity=25.0521920668058, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786307, Length=476, Percent_Identity=33.6134453781513, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI87082354, Length=476, Percent_Identity=30.0420168067227, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87081717, Length=469, Percent_Identity=28.7846481876333, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1789915, Length=445, Percent_Identity=25.8426966292135, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=466, Percent_Identity=41.8454935622318, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17557007, Length=490, Percent_Identity=25.1020408163265, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71983429, Length=462, Percent_Identity=25.974025974026, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=462, Percent_Identity=25.974025974026, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=491, Percent_Identity=24.847250509165, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321091, Length=480, Percent_Identity=39.1666666666667, Blast_Score=282, Evalue=9e-77,
Organism=Saccharomyces cerevisiae, GI6325240, Length=483, Percent_Identity=28.1573498964803, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325166, Length=475, Percent_Identity=25.4736842105263, Blast_Score=147, Evalue=4e-36,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=40.5117270788913, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24640549, Length=487, Percent_Identity=26.8993839835729, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24640553, Length=487, Percent_Identity=26.8993839835729, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24640551, Length=487, Percent_Identity=26.8993839835729, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI17737741, Length=488, Percent_Identity=26.6393442622951, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50872; Mature: 50740

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEYDVVVLGGGTGGYVAAIQAAKNGQKVAVVEKGKVGGTCLHRGCIPTKALLRSAEVL
CCCCEEEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHH
QTVKKASEFGVSVEGNAGINFLQAQERKQQIVDQLEKGIHQLFKQGKIDLFVGTGTILGP
HHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECC
SIFSPTAGTVSVELEDGSENEMLIPKNLIIATGSKPRTLNGLTIDEENVLSSDGALNLET
CCCCCCCCEEEEEECCCCCCCEECCCCEEEECCCCCCEECCCEECCCHHCCCCCCEEHHH
LPKSILIVGGGVIGMEWASMLHDFGVEVTVLEYADRILPTEDKEVAKELARLYKKKNLTM
CCCEEEEEECCCHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHCCCEE
HTSAEVQAASYKKTNNGVEIKAIIKGEEQTFSADKILVSVGRSANTENIGLQNTDIATEN
EECCCEEEHHCEECCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCEECCC
GFIQVNDFYQTKESHIYAIGDCIPTIQLAHVAMEEGTIAANHIAGKATEKLDYDLVPRCI
CEEEEECCCCCCCCCEEEECCHHCHHHHHHHHHHCCCEEEHHHCCCHHHHCCCCCCCCEE
YTSTEIASVGITEEQAKERGHDVKKGKFFFRGIGKALVYGESDGFIKIIADKKTDDILGV
ECCCHHEECCCCHHHHHHCCCCCCCCCHHEEECCCEEEEECCCCEEEEEECCCCCCEEEE
SMIGPHVTDMISEAALAQVLNATPWEVGNTIHPHPTLAESFREAALAVDGNAIHG
ECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCC
>Mature Secondary Structure 
ATEYDVVVLGGGTGGYVAAIQAAKNGQKVAVVEKGKVGGTCLHRGCIPTKALLRSAEVL
CCCEEEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHH
QTVKKASEFGVSVEGNAGINFLQAQERKQQIVDQLEKGIHQLFKQGKIDLFVGTGTILGP
HHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECC
SIFSPTAGTVSVELEDGSENEMLIPKNLIIATGSKPRTLNGLTIDEENVLSSDGALNLET
CCCCCCCCEEEEEECCCCCCCEECCCCEEEECCCCCCEECCCEECCCHHCCCCCCEEHHH
LPKSILIVGGGVIGMEWASMLHDFGVEVTVLEYADRILPTEDKEVAKELARLYKKKNLTM
CCCEEEEEECCCHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHCCCEE
HTSAEVQAASYKKTNNGVEIKAIIKGEEQTFSADKILVSVGRSANTENIGLQNTDIATEN
EECCCEEEHHCEECCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCEECCC
GFIQVNDFYQTKESHIYAIGDCIPTIQLAHVAMEEGTIAANHIAGKATEKLDYDLVPRCI
CEEEEECCCCCCCCCEEEECCHHCHHHHHHHHHHCCCEEEHHHCCCHHHHCCCCCCCCEE
YTSTEIASVGITEEQAKERGHDVKKGKFFFRGIGKALVYGESDGFIKIIADKKTDDILGV
ECCCHHEECCCCHHHHHHCCCCCCCCCHHEEECCCEEEEECCCCEEEEEECCCCCCEEEE
SMIGPHVTDMISEAALAQVLNATPWEVGNTIHPHPTLAESFREAALAVDGNAIHG
ECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8504804 [H]