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The map label for this gene is bfmBAA [H]

Identifier: 116872803

GI number: 116872803

Start: 1374991

End: 1375986

Strand: Direct

Name: bfmBAA [H]

Synonym: lwe1387

Alternate gene names: 116872803

Gene position: 1374991-1375986 (Clockwise)

Preceding gene: 116872802

Following gene: 116872804

Centisome position: 48.86

GC content: 39.86

Gene sequence:

>996_bases
ATGACTTTAAAAGAAGCAGGTTTAACAGAAGATAAATTAATTAAAATGTATGAAACAATGTTAATGGCAAGAAGACTTGA
TGAGCGGATGTGGTTGTTAAACCGTTCAGGGAAAATCCCTTTCACCATTTCTGGGCAAGGACAAGAAACCGCACAAATTG
GTGCTGCATTTGCCTTTGATTTAGATAAAGATTATGCATTACCGTATTATCGTGATTTGGCAGTTGTGTTATCGTTTGGG
ATGACGGCGAAAGATATTATGCTATCAGCCTTTGCTAAAGCGGAAGATCCAAACTCTGGCGGACGTCAAATGCCAGCTCA
TTTTGGTCAAAAATCAAATCGTATCGTCACTCAAAGTTCACCTGTAACAACACAATTTCCGCATGCAGCAGGGATTGGTC
TTGCAGCAAAAATGGCTGGGGATAAAATTGCGATTTATGCTTCTACAGGTGAAGGGTCTTCTAACCAAGGAGATTTCCAT
GAAGGAATCAACTTCGCATCTGTACATAAGTTGCCAGTTGTTTTCGTCATCCACAACAATAAGTACGCTATTTCAGTACC
AGCATCCAAGCAGTATGCAGCAGAAAAACTATCAGACCGAGCAATTGGTTATGGTATTCCTGGTGAACGCGTGGATGGTA
CAAATATGAGTGAAGTATATGGAGCCTTTAAACGCGCAGCAGATCGTGCACGAAACGGCGAAGGGCCCACACTAATTGAA
ACCGTTTCTTATCGCTTTACACCGCACTCCTCAGATGATGACGATAGTAGTTATCGTTCTAGAGAAGAAGTAGATGAAGC
GAAAGTAAAAGATCCACTGAAAATCTTCCAAAGAGAATTGCTAGAAGAAGGTTATTTAACAGAAGAAAAAATCGCTGAAA
TTGAAAAAAATATTGCTAAAGAAGTTAATGAAGCAACTGATTATGCGGAAAGTGCAGCATACGCTGAACCAGAATCATCT
TTACTTTATGTATATGATGAAGAAGCGAATAGCTGA

Upstream 100 bases:

>100_bases
TCACCCGCACCCAACTTTAGCAGAAAGTTTTAGAGAAGCTGCCCTTGCTGTGGATGGTAACGCGATTCATGGTTAATACA
GCTTTAAAGGAGGAACTAAA

Downstream 100 bases:

>100_bases
TTAGGAGGGAATTTGAATGCCAGTCATTTCATATATTGATGCAATAACAATGGCGCTTAAAGAAGAAATGGAGCGCGATG
ATAAAGTATTTATTTTAGGG

Product: branched-chain alpha-keto acid dehydrogenase E1 (2-oxoisovalerate dehydrogenase alpha subunit)

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTLKEAGLTEDKLIKMYETMLMARRLDERMWLLNRSGKIPFTISGQGQETAQIGAAFAFDLDKDYALPYYRDLAVVLSFG
MTAKDIMLSAFAKAEDPNSGGRQMPAHFGQKSNRIVTQSSPVTTQFPHAAGIGLAAKMAGDKIAIYASTGEGSSNQGDFH
EGINFASVHKLPVVFVIHNNKYAISVPASKQYAAEKLSDRAIGYGIPGERVDGTNMSEVYGAFKRAADRARNGEGPTLIE
TVSYRFTPHSSDDDDSSYRSREEVDEAKVKDPLKIFQRELLEEGYLTEEKIAEIEKNIAKEVNEATDYAESAAYAEPESS
LLYVYDEEANS

Sequences:

>Translated_331_residues
MTLKEAGLTEDKLIKMYETMLMARRLDERMWLLNRSGKIPFTISGQGQETAQIGAAFAFDLDKDYALPYYRDLAVVLSFG
MTAKDIMLSAFAKAEDPNSGGRQMPAHFGQKSNRIVTQSSPVTTQFPHAAGIGLAAKMAGDKIAIYASTGEGSSNQGDFH
EGINFASVHKLPVVFVIHNNKYAISVPASKQYAAEKLSDRAIGYGIPGERVDGTNMSEVYGAFKRAADRARNGEGPTLIE
TVSYRFTPHSSDDDDSSYRSREEVDEAKVKDPLKIFQRELLEEGYLTEEKIAEIEKNIAKEVNEATDYAESAAYAEPESS
LLYVYDEEANS
>Mature_330_residues
TLKEAGLTEDKLIKMYETMLMARRLDERMWLLNRSGKIPFTISGQGQETAQIGAAFAFDLDKDYALPYYRDLAVVLSFGM
TAKDIMLSAFAKAEDPNSGGRQMPAHFGQKSNRIVTQSSPVTTQFPHAAGIGLAAKMAGDKIAIYASTGEGSSNQGDFHE
GINFASVHKLPVVFVIHNNKYAISVPASKQYAAEKLSDRAIGYGIPGERVDGTNMSEVYGAFKRAADRARNGEGPTLIET
VSYRFTPHSSDDDDSSYRSREEVDEAKVKDPLKIFQRELLEEGYLTEEKIAEIEKNIAKEVNEATDYAESAAYAEPESSL
LYVYDEEANS

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=323, Percent_Identity=36.2229102167183, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI258645172, Length=323, Percent_Identity=36.2229102167183, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI4885543, Length=316, Percent_Identity=31.3291139240506, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4505685, Length=316, Percent_Identity=29.746835443038, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI291084742, Length=316, Percent_Identity=29.746835443038, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI291084744, Length=323, Percent_Identity=29.1021671826625, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI291084757, Length=167, Percent_Identity=34.7305389221557, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI86563357, Length=327, Percent_Identity=36.3914373088685, Blast_Score=194, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI86563355, Length=327, Percent_Identity=36.3914373088685, Blast_Score=194, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17536047, Length=320, Percent_Identity=30, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32564172, Length=320, Percent_Identity=30, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321026, Length=318, Percent_Identity=30.188679245283, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI21355903, Length=295, Percent_Identity=31.1864406779661, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24639740, Length=316, Percent_Identity=32.2784810126582, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24639744, Length=316, Percent_Identity=32.2784810126582, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI28571106, Length=316, Percent_Identity=32.2784810126582, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24639746, Length=268, Percent_Identity=31.7164179104478, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24639748, Length=306, Percent_Identity=29.4117647058824, Blast_Score=110, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 36506; Mature: 36375

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKEAGLTEDKLIKMYETMLMARRLDERMWLLNRSGKIPFTISGQGQETAQIGAAFAFD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHEEEEEE
LDKDYALPYYRDLAVVLSFGMTAKDIMLSAFAKAEDPNSGGRQMPAHFGQKSNRIVTQSS
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEEEECCC
PVTTQFPHAAGIGLAAKMAGDKIAIYASTGEGSSNQGDFHEGINFASVHKLPVVFVIHNN
CCCCCCCCHHCCCHHEEECCCEEEEEEECCCCCCCCCCHHHCCCCCEEECCEEEEEEECC
KYAISVPASKQYAAEKLSDRAIGYGIPGERVDGTNMSEVYGAFKRAADRARNGEGPTLIE
EEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
TVSYRFTPHSSDDDDSSYRSREEVDEAKVKDPLKIFQRELLEEGYLTEEKIAEIEKNIAK
EEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EVNEATDYAESAAYAEPESSLLYVYDEEANS
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
TLKEAGLTEDKLIKMYETMLMARRLDERMWLLNRSGKIPFTISGQGQETAQIGAAFAFD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHEEEEEE
LDKDYALPYYRDLAVVLSFGMTAKDIMLSAFAKAEDPNSGGRQMPAHFGQKSNRIVTQSS
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEEEECCC
PVTTQFPHAAGIGLAAKMAGDKIAIYASTGEGSSNQGDFHEGINFASVHKLPVVFVIHNN
CCCCCCCCHHCCCHHEEECCCEEEEEEECCCCCCCCCCHHHCCCCCEEECCEEEEEEECC
KYAISVPASKQYAAEKLSDRAIGYGIPGERVDGTNMSEVYGAFKRAADRARNGEGPTLIE
EEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
TVSYRFTPHSSDDDDSSYRSREEVDEAKVKDPLKIFQRELLEEGYLTEEKIAEIEKNIAK
EEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EVNEATDYAESAAYAEPESSLLYVYDEEANS
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377 [H]