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The map label for this gene is ribF [H]

Identifier: 116872760

GI number: 116872760

Start: 1336596

End: 1337540

Strand: Direct

Name: ribF [H]

Synonym: lwe1344

Alternate gene names: 116872760

Gene position: 1336596-1337540 (Clockwise)

Preceding gene: 116872759

Following gene: 116872761

Centisome position: 47.5

GC content: 37.57

Gene sequence:

>945_bases
ATGAAGACGATATACTTACATCATCCAATTAAAGTGGATGAGTGGACAGATGTAAAAAAAGTAATGGCACTTGGCTTCTT
TGATGGTGTTCATTTAGGACATCAAGCAGTCATTAAAAAAGCGAAACAGATTGCGGAACAAAAAGGACTTCAAACAGCAG
TCTTAACTTTTGACCCACATCCATCTGTCGTTTTAAGCAACATCCGAAAACAAGTAAAATATTTAACTCCACTGGAAGAT
AAAGCAGAAAAAATGGCGGAACTTGGAGTAGATATTATGTACGTAGTTCGCTTTACTACACAGTTTTCCGAATTATCCCC
GCAAGCATTTGTGGACAAATATCTCGTTTCTTTAAATGTAAGTCATGTAGTAGCAGGTTTTGACTACTCTTATGGTAAAA
AAGGGGAAGGTAAAATGGCCGATTTAGAACGTTATGCAAATGGCCGTTTTGACGTTACTATTGTGGATAAACAGACTGCT
GCTAGTGATAAAATAAGCTCAACCAACATTAGACGTGCGATTTCGGAAGGCGAGCTGGAAGAAGCAAATCAATTACTGGG
CTATCCTTACACAACAAAAGGAACGGTCATTCACGGAGATAAACGTGGTAGGACAATTGGATTTCCAACAGCGAATATTC
TTGTTAATGAAGATTATTTAATTCCAAAACTTGGCGTTTATGCAGTGAAATTCCGTGTGAACGGGGAAACGCATCTTGGT
ATGGCAAGTATTGGGTATAATATTACTTTTAAAGATGATCAAGCATTATCGATTGAAGTATATATTTTGGATTTTCACTG
GGAAATTTACGGCGAAGAAGCAGAAATCGAATGGTACCAGTTTTTCCGTCCAGAGCTGAAATTTAATGGGGTGGAAGGTC
TGATTGCTCAATTAGAAAAAGACGAGCAAGATACAAGAGCTTTTTTTGCTGATTTAGAGGATTAA

Upstream 100 bases:

>100_bases
GAAAAAGTCATTGAATTAAATGAAGCATAGTTATTGCGGCTACAACTTTCTTATATTATGATAATAGAGGAATTCGAATT
TTGAGGAGAAGTGGCAAAAA

Downstream 100 bases:

>100_bases
ACTACTATTTTTGCTTGCTTTTGGAGGCCAAATAGTGTATCTTTAAATCTGTACGAACAGAACCATTACTTGGCTTTTGC
GACTCACCGACGCTTGGCTC

Product: riboflavin biosynthesis protein RibF

Products: NA

Alternate protein names: Riboflavin kinase; Flavokinase; FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKTIYLHHPIKVDEWTDVKKVMALGFFDGVHLGHQAVIKKAKQIAEQKGLQTAVLTFDPHPSVVLSNIRKQVKYLTPLED
KAEKMAELGVDIMYVVRFTTQFSELSPQAFVDKYLVSLNVSHVVAGFDYSYGKKGEGKMADLERYANGRFDVTIVDKQTA
ASDKISSTNIRRAISEGELEEANQLLGYPYTTKGTVIHGDKRGRTIGFPTANILVNEDYLIPKLGVYAVKFRVNGETHLG
MASIGYNITFKDDQALSIEVYILDFHWEIYGEEAEIEWYQFFRPELKFNGVEGLIAQLEKDEQDTRAFFADLED

Sequences:

>Translated_314_residues
MKTIYLHHPIKVDEWTDVKKVMALGFFDGVHLGHQAVIKKAKQIAEQKGLQTAVLTFDPHPSVVLSNIRKQVKYLTPLED
KAEKMAELGVDIMYVVRFTTQFSELSPQAFVDKYLVSLNVSHVVAGFDYSYGKKGEGKMADLERYANGRFDVTIVDKQTA
ASDKISSTNIRRAISEGELEEANQLLGYPYTTKGTVIHGDKRGRTIGFPTANILVNEDYLIPKLGVYAVKFRVNGETHLG
MASIGYNITFKDDQALSIEVYILDFHWEIYGEEAEIEWYQFFRPELKFNGVEGLIAQLEKDEQDTRAFFADLED
>Mature_314_residues
MKTIYLHHPIKVDEWTDVKKVMALGFFDGVHLGHQAVIKKAKQIAEQKGLQTAVLTFDPHPSVVLSNIRKQVKYLTPLED
KAEKMAELGVDIMYVVRFTTQFSELSPQAFVDKYLVSLNVSHVVAGFDYSYGKKGEGKMADLERYANGRFDVTIVDKQTA
ASDKISSTNIRRAISEGELEEANQLLGYPYTTKGTVIHGDKRGRTIGFPTANILVNEDYLIPKLGVYAVKFRVNGETHLG
MASIGYNITFKDDQALSIEVYILDFHWEIYGEEAEIEWYQFFRPELKFNGVEGLIAQLEKDEQDTRAFFADLED

Specific function: Unknown

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribF family [H]

Homologues:

Organism=Escherichia coli, GI1786208, Length=293, Percent_Identity=34.8122866894198, Blast_Score=167, Evalue=6e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004821
- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729 [H]

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase [H]

EC number: =2.7.1.26; =2.7.7.2 [H]

Molecular weight: Translated: 35591; Mature: 35591

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIYLHHPIKVDEWTDVKKVMALGFFDGVHLGHQAVIKKAKQIAEQKGLQTAVLTFDPH
CCEEEEECCCCCCCHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHCCCEEEEEEECCC
PSVVLSNIRKQVKYLTPLEDKAEKMAELGVDIMYVVRFTTQFSELSPQAFVDKYLVSLNV
CHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHCCH
SHVVAGFDYSYGKKGEGKMADLERYANGRFDVTIVDKQTAASDKISSTNIRRAISEGELE
HHEEEECCCCCCCCCCCCHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCHH
EANQLLGYPYTTKGTVIHGDKRGRTIGFPTANILVNEDYLIPKLGVYAVKFRVNGETHLG
HHHHHHCCCCCCCCEEEECCCCCCEECCCCEEEEEECCCCCCCCCEEEEEEEECCCCEEE
MASIGYNITFKDDQALSIEVYILDFHWEIYGEEAEIEWYQFFRPELKFNGVEGLIAQLEK
EEECCEEEEECCCCEEEEEEEEEEEEEEEECCCCCEEHEEHCCCCCEECCHHHHHHHHCC
DEQDTRAFFADLED
CCHHHHHHHCCCCC
>Mature Secondary Structure
MKTIYLHHPIKVDEWTDVKKVMALGFFDGVHLGHQAVIKKAKQIAEQKGLQTAVLTFDPH
CCEEEEECCCCCCCHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHCCCEEEEEEECCC
PSVVLSNIRKQVKYLTPLEDKAEKMAELGVDIMYVVRFTTQFSELSPQAFVDKYLVSLNV
CHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHCCH
SHVVAGFDYSYGKKGEGKMADLERYANGRFDVTIVDKQTAASDKISSTNIRRAISEGELE
HHEEEECCCCCCCCCCCCHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCHH
EANQLLGYPYTTKGTVIHGDKRGRTIGFPTANILVNEDYLIPKLGVYAVKFRVNGETHLG
HHHHHHCCCCCCCCEEEECCCCCCEECCCCEEEEEECCCCCCCCCEEEEEEEECCCCEEE
MASIGYNITFKDDQALSIEVYILDFHWEIYGEEAEIEWYQFFRPELKFNGVEGLIAQLEK
EEECCEEEEECCCCEEEEEEEEEEEEEEEECCCCCEEHEEHCCCCCEECCHHHHHHHHCC
DEQDTRAFFADLED
CCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9454067; 9108293; 9384377 [H]