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The map label for this gene is truB

Identifier: 116872759

GI number: 116872759

Start: 1335611

End: 1336525

Strand: Direct

Name: truB

Synonym: lwe1343

Alternate gene names: 116872759

Gene position: 1335611-1336525 (Clockwise)

Preceding gene: 116872758

Following gene: 116872760

Centisome position: 47.46

GC content: 37.05

Gene sequence:

>915_bases
ATGAACGGCATTATCCCTTTGTGGAAAGAACGCGGGATGACGAGTCATGATTGCGTTTTTAAATTAAGAAAAATTCTACA
TACAAAAAAAGTTGGACATACCGGGACACTCGATCCAGAAGTGGAAGGTGTACTCCCAATTTGTATCGGACGAGCGACAA
AACTGGCGGAATATGTAACAGATGAAGGAAAAGTTTATGTAGCTGAAATAACATTAGGTAAATCAACTACAACAGAAGAT
GCCACTGGCGAAATCATAGCAACAAAAGATTTAGCAGAAATTTCACCAGAAGCACTTCAATCCGTACTCACTAAGCTTAC
AGGAAAAATCACCCAAATTCCGCCGATGTTTTCAGCTGTAAAAGTGAACGGGAAGAAATTGTATGAATATGCAAGAGCAG
GAATAGAGGTAGAACGACCATCCAGACAAGTGGATATTTATTCATTAACCCGTTTAGATGGCCTCGCTTCTCTAAATGAA
TCAAATCCTACTTTTCAATTAGAAATCTCTTGTGGAAAAGGGACATATATCCGTACACTCGCAGTCATGATTGGTGAATT
GTTAGGATATCCAGCACATATGTCCAAACTAGAACGTACCCGAAGTGGCTTTTTTAAAAAAGAAGATTGTTTCACCTTGT
CTGAAATTGACGAAATGATGCAAGCGAATGATCTCAATTTTTTATATCCACTTGAAAAAGGGATTGAATCCATGACAAAA
CTAATTATTGATGATGAAATACATGCAAAAGTTTTAAATGGCGCATTATTACCAAAGGCATTATTTGTATCGGTTGAAAA
TGAATCTCGTGTCGCGCTTATTTTTGAAGGTAAATTAACTGCAATTTACAAGCCACATCCGGAGAAAAAAGACCTTTGGA
AACCAGAAAAAGTCATTGAATTAAATGAAGCATAG

Upstream 100 bases:

>100_bases
ATAATAAATTAAAGTTTAAGGCAATCCCTTTATCTTCTCAGTCAAACGGGATATAAATGGAGAATTGTCTTAAACTTTTT
TTCTTAGAAGGAGGAGCTTG

Downstream 100 bases:

>100_bases
TTATTGCGGCTACAACTTTCTTATATTATGATAATAGAGGAATTCGAATTTTGAGGAGAAGTGGCAAAAAATGAAGACGA
TATACTTACATCATCCAATT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MNGIIPLWKERGMTSHDCVFKLRKILHTKKVGHTGTLDPEVEGVLPICIGRATKLAEYVTDEGKVYVAEITLGKSTTTED
ATGEIIATKDLAEISPEALQSVLTKLTGKITQIPPMFSAVKVNGKKLYEYARAGIEVERPSRQVDIYSLTRLDGLASLNE
SNPTFQLEISCGKGTYIRTLAVMIGELLGYPAHMSKLERTRSGFFKKEDCFTLSEIDEMMQANDLNFLYPLEKGIESMTK
LIIDDEIHAKVLNGALLPKALFVSVENESRVALIFEGKLTAIYKPHPEKKDLWKPEKVIELNEA

Sequences:

>Translated_304_residues
MNGIIPLWKERGMTSHDCVFKLRKILHTKKVGHTGTLDPEVEGVLPICIGRATKLAEYVTDEGKVYVAEITLGKSTTTED
ATGEIIATKDLAEISPEALQSVLTKLTGKITQIPPMFSAVKVNGKKLYEYARAGIEVERPSRQVDIYSLTRLDGLASLNE
SNPTFQLEISCGKGTYIRTLAVMIGELLGYPAHMSKLERTRSGFFKKEDCFTLSEIDEMMQANDLNFLYPLEKGIESMTK
LIIDDEIHAKVLNGALLPKALFVSVENESRVALIFEGKLTAIYKPHPEKKDLWKPEKVIELNEA
>Mature_304_residues
MNGIIPLWKERGMTSHDCVFKLRKILHTKKVGHTGTLDPEVEGVLPICIGRATKLAEYVTDEGKVYVAEITLGKSTTTED
ATGEIIATKDLAEISPEALQSVLTKLTGKITQIPPMFSAVKVNGKKLYEYARAGIEVERPSRQVDIYSLTRLDGLASLNE
SNPTFQLEISCGKGTYIRTLAVMIGELLGYPAHMSKLERTRSGFFKKEDCFTLSEIDEMMQANDLNFLYPLEKGIESMTK
LIIDDEIHAKVLNGALLPKALFVSVENESRVALIFEGKLTAIYKPHPEKKDLWKPEKVIELNEA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=256, Percent_Identity=33.203125, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI4503337, Length=271, Percent_Identity=32.1033210332103, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI215599015, Length=271, Percent_Identity=32.1033210332103, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI2367200, Length=221, Percent_Identity=42.5339366515837, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17553978, Length=271, Percent_Identity=27.6752767527675, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6323204, Length=265, Percent_Identity=30.9433962264151, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324037, Length=197, Percent_Identity=35.5329949238579, Blast_Score=101, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281364189, Length=278, Percent_Identity=31.294964028777, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI281364187, Length=278, Percent_Identity=31.294964028777, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI281364185, Length=278, Percent_Identity=31.294964028777, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI281364183, Length=278, Percent_Identity=31.294964028777, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI62471759, Length=278, Percent_Identity=31.294964028777, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI17975520, Length=278, Percent_Identity=31.294964028777, Blast_Score=98, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_LISW6 (A0AIC9)

Other databases:

- EMBL:   AM263198
- RefSeq:   YP_849540.1
- ProteinModelPortal:   A0AIC9
- SMR:   A0AIC9
- STRING:   A0AIC9
- GeneID:   4465799
- GenomeReviews:   AM263198_GR
- KEGG:   lwe:lwe1343
- NMPDR:   fig|386043.6.peg.1302
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- PhylomeDB:   A0AIC9
- ProtClustDB:   PRK01550
- BioCyc:   LWEL386043:LWE1343-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 33859; Mature: 33859

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGIIPLWKERGMTSHDCVFKLRKILHTKKVGHTGTLDPEVEGVLPICIGRATKLAEYVT
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
DEGKVYVAEITLGKSTTTEDATGEIIATKDLAEISPEALQSVLTKLTGKITQIPPMFSAV
CCCCEEEEEEEECCCCCCCCCCCCEEEECCHHHCCHHHHHHHHHHHCCCHHCCCCCHHHE
KVNGKKLYEYARAGIEVERPSRQVDIYSLTRLDGLASLNESNPTFQLEISCGKGTYIRTL
EECHHHHHHHHHCCCEEECCCCEEEEEEHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHH
AVMIGELLGYPAHMSKLERTRSGFFKKEDCFTLSEIDEMMQANDLNFLYPLEKGIESMTK
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCEEEHHHHHHHHHHH
LIIDDEIHAKVLNGALLPKALFVSVENESRVALIFEGKLTAIYKPHPEKKDLWKPEKVIE
HHHCCHHHHHHHCCHHCCEEEEEEECCCCEEEEEEECCEEEEECCCCCCCCCCCCHHHEE
LNEA
ECCC
>Mature Secondary Structure
MNGIIPLWKERGMTSHDCVFKLRKILHTKKVGHTGTLDPEVEGVLPICIGRATKLAEYVT
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
DEGKVYVAEITLGKSTTTEDATGEIIATKDLAEISPEALQSVLTKLTGKITQIPPMFSAV
CCCCEEEEEEEECCCCCCCCCCCCEEEECCHHHCCHHHHHHHHHHHCCCHHCCCCCHHHE
KVNGKKLYEYARAGIEVERPSRQVDIYSLTRLDGLASLNESNPTFQLEISCGKGTYIRTL
EECHHHHHHHHHCCCEEECCCCEEEEEEHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHH
AVMIGELLGYPAHMSKLERTRSGFFKKEDCFTLSEIDEMMQANDLNFLYPLEKGIESMTK
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCEEEHHHHHHHHHHH
LIIDDEIHAKVLNGALLPKALFVSVENESRVALIFEGKLTAIYKPHPEKKDLWKPEKVIE
HHHCCHHHHHHHCCHHCCEEEEEEECCCCEEEEEEECCEEEEECCCCCCCCCCCCHHHEE
LNEA
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA