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The map label for this gene is glpF [H]

Identifier: 116872541

GI number: 116872541

Start: 1137170

End: 1137874

Strand: Direct

Name: glpF [H]

Synonym: lwe1125

Alternate gene names: 116872541

Gene position: 1137170-1137874 (Clockwise)

Preceding gene: 116872540

Following gene: 116872542

Centisome position: 40.41

GC content: 40.99

Gene sequence:

>705_bases
ATGTCAGCATATTTGGCGGAATTTATTGGTACGATGGTTTTGATTATGTTTGGGAATGGCCTTTTAGCAGGATTAACCTT
GAATAAATCTTTATCGCAAGGTGCAAACTGGGTAGTTGTTACTTTTGGTTGGGGTTTTGCTGTAATGATTGGGGTTTATG
TAGCTGGAGCATATAGCGGGGCACATTTAAACCCAGCCGTAACCATTGCTCTTGCGGTCGGAGGGTCTTTCCCTTGGGCA
GATGTGGTTCCGTATATTATCGCGCAAATCGCCGGAGCATTTGTCGGAGCATCCATTGTTATCTTACATTACTATCCGCA
TTTCAAAGCGACTCCAAGAGAGATTGATACACATGGTATTTTCTCTACTGGACCAGCGATTCGTAATACACCATTCAATC
TTATCAGTGAAATTATCGCAACATTTGCCTTTATTTTTGGTTTACTTATGATTGGAGAGAATAGTTTTACAGATGGTTTA
AATCCGTTAATCCTTGGCTTTCTAGTAGTTGCGATTGGGATGAGTTTTGGACCAACAACAGGTTATGCAATTAACCCAGC
ACGTGACCTTGGACCAAGACTTGCTTACTTTTTACTACCAGTTCCAAATAAAAGTGGATCAGATTGGCGTTATGCATGGA
TTCCTATTGTGGGGCCAATCATTGGCGGGTTACTTGCCATCGGACTGTATAACATTTTATTATAA

Upstream 100 bases:

>100_bases
CGCTTGTTTACCTACTAGTCCAACAACACCGTCACATCAGGAACTAAAAGAAATTTTAGAACAAATTATTTAGATTTTAC
GTAAAAAAGGAGGATAAACT

Downstream 100 bases:

>100_bases
AAAATAAGATGGAGTAAACAATGATTAGATTGTTTGCTCCTTTAAAAATGAGAAAGGACTGAGCTATATGCACAAAATTA
TGGCGATAAACGCAGGGAGT

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSAYLAEFIGTMVLIMFGNGLLAGLTLNKSLSQGANWVVVTFGWGFAVMIGVYVAGAYSGAHLNPAVTIALAVGGSFPWA
DVVPYIIAQIAGAFVGASIVILHYYPHFKATPREIDTHGIFSTGPAIRNTPFNLISEIIATFAFIFGLLMIGENSFTDGL
NPLILGFLVVAIGMSFGPTTGYAINPARDLGPRLAYFLLPVPNKSGSDWRYAWIPIVGPIIGGLLAIGLYNILL

Sequences:

>Translated_234_residues
MSAYLAEFIGTMVLIMFGNGLLAGLTLNKSLSQGANWVVVTFGWGFAVMIGVYVAGAYSGAHLNPAVTIALAVGGSFPWA
DVVPYIIAQIAGAFVGASIVILHYYPHFKATPREIDTHGIFSTGPAIRNTPFNLISEIIATFAFIFGLLMIGENSFTDGL
NPLILGFLVVAIGMSFGPTTGYAINPARDLGPRLAYFLLPVPNKSGSDWRYAWIPIVGPIIGGLLAIGLYNILL
>Mature_233_residues
SAYLAEFIGTMVLIMFGNGLLAGLTLNKSLSQGANWVVVTFGWGFAVMIGVYVAGAYSGAHLNPAVTIALAVGGSFPWAD
VVPYIIAQIAGAFVGASIVILHYYPHFKATPREIDTHGIFSTGPAIRNTPFNLISEIIATFAFIFGLLMIGENSFTDGLN
PLILGFLVVAIGMSFGPTTGYAINPARDLGPRLAYFLLPVPNKSGSDWRYAWIPIVGPIIGGLLAIGLYNILL

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=248, Percent_Identity=36.2903225806452, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI4502187, Length=244, Percent_Identity=35.2459016393443, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI22538420, Length=246, Percent_Identity=32.520325203252, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI157266307, Length=236, Percent_Identity=34.3220338983051, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI4755125, Length=236, Percent_Identity=32.2033898305085, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI4502181, Length=236, Percent_Identity=32.2033898305085, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI310133356, Length=220, Percent_Identity=31.8181818181818, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI310114181, Length=220, Percent_Identity=31.8181818181818, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1790362, Length=256, Percent_Identity=36.328125, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1787101, Length=189, Percent_Identity=29.6296296296296, Blast_Score=63, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71992966, Length=248, Percent_Identity=33.0645161290323, Blast_Score=117, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17531429, Length=244, Percent_Identity=34.4262295081967, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17531431, Length=244, Percent_Identity=34.4262295081967, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71994009, Length=232, Percent_Identity=32.3275862068966, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32564052, Length=241, Percent_Identity=34.0248962655602, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17533613, Length=241, Percent_Identity=34.0248962655602, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17544068, Length=248, Percent_Identity=34.2741935483871, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71992961, Length=244, Percent_Identity=30.327868852459, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17558372, Length=238, Percent_Identity=28.1512605042017, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71993722, Length=238, Percent_Identity=27.7310924369748, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321054, Length=210, Percent_Identity=40.4761904761905, Blast_Score=129, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6322985, Length=284, Percent_Identity=28.5211267605634, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652747, Length=236, Percent_Identity=28.8135593220339, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI45551084, Length=236, Percent_Identity=28.8135593220339, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19922828, Length=202, Percent_Identity=26.7326732673267, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24828; Mature: 24696

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAYLAEFIGTMVLIMFGNGLLAGLTLNKSLSQGANWVVVTFGWGFAVMIGVYVAGAYSG
CCHHHHHHHHHHHHHHHCCCEEEEEEECHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCC
AHLNPAVTIALAVGGSFPWADVVPYIIAQIAGAFVGASIVILHYYPHFKATPREIDTHGI
CCCCHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCC
FSTGPAIRNTPFNLISEIIATFAFIFGLLMIGENSFTDGLNPLILGFLVVAIGMSFGPTT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
GYAINPARDLGPRLAYFLLPVPNKSGSDWRYAWIPIVGPIIGGLLAIGLYNILL
CCEECCHHHHCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAYLAEFIGTMVLIMFGNGLLAGLTLNKSLSQGANWVVVTFGWGFAVMIGVYVAGAYSG
CHHHHHHHHHHHHHHHCCCEEEEEEECHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCC
AHLNPAVTIALAVGGSFPWADVVPYIIAQIAGAFVGASIVILHYYPHFKATPREIDTHGI
CCCCHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCC
FSTGPAIRNTPFNLISEIIATFAFIFGLLMIGENSFTDGLNPLILGFLVVAIGMSFGPTT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
GYAINPARDLGPRLAYFLLPVPNKSGSDWRYAWIPIVGPIIGGLLAIGLYNILL
CCEECCHHHHCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8436953; 9579061; 9384377; 2127799 [H]