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The map label for this gene is adh1 [H]

Identifier: 116872540

GI number: 116872540

Start: 1136024

End: 1137142

Strand: Direct

Name: adh1 [H]

Synonym: lwe1124

Alternate gene names: 116872540

Gene position: 1136024-1137142 (Clockwise)

Preceding gene: 116872539

Following gene: 116872541

Centisome position: 40.37

GC content: 37.8

Gene sequence:

>1119_bases
ATGAATAGCTTTCAAATCAAGACAAAAGTAGCTTTTGGTACGAATAGTTTACAAGTATTAAAAGAAATTAAAAATAAAAA
TGTCTGGATTATTTGTGACCGATTTTTAGCAGATGGAGAGGGACTTCAAGGATTAATTGGACAGTTAGATGTATCCAACA
ATGTGCATATTTTCACCGACGTTGTTCCAGATCCGCCAATCTCTAAAGTAGCTAGCGGTGTCAGTGAAGCAGGCAAAATC
CAACCACAAGTAATGATAGCTTTTGGTGGCGGTTCAGCGATTGATACAGCTAAAGGAATCTACTACTTCGCCAAACGGTT
GGAGAAAATCAATATAAGTACTTTTATAGCGATTCCAACGACGAGTGGAACTGGATCTGAAGTAACAGCTGCAACCGTCA
TTACTGATCCAACAACAAAAATTAAATATCCACTTTTCTTAGATGAACTTATTCCAGATATGGCTATTTTAGATGCACAA
CTTGTTGTCACAGTGCCACCAGCGATTACCGCGAATACTGGAATGGACGTGTTGACACATGCGATTGAAGCTTATGTTTC
TAAAGCTGCAAATGATTATACTGATGCTCTAGGTGAAAAAAGTGTTCAGTTAACACTAGGTTTCTTAACGAGTTGTTATG
ATGACGGACGTAATTTGGCTAATCGAGAAAAAATGCATAATGCTTCCACGATGGCGGGAATGGCATTTAACTGCGCAAAC
CTTGGATTAAATCATAGTATTGCTCATCAATTAGGTGCTCAATTTCATGTACCTCATGGTTTAGCAAATGCAATTTTACT
TGATGCGGTTATTCGGTTTAATGCATTTAAAAATCGCGATACGGAACAAAAATATGCTGAAATGGCTCGGATTTGTGGAA
TTGCTTCAAGATCTGATTCGAATGAGACTGCTGTCCGACTTCTTCGTGAAAGAATTGTCCAAATGATGGAACATATGCAA
ATGCCGCGCACTTTAACAGATGCTGGTGTAGCCAAAGAAAAAGTATATGCAAAAATGGACGAAATTGCTACAAATGCACT
TAAAGACGCTTGTTTACCTACTAGTCCAACAACACCGTCACATCAGGAACTAAAAGAAATTTTAGAACAAATTATTTAG

Upstream 100 bases:

>100_bases
CCCAACTGGAGAAGGAACCACTACAGCACGTCATTTTGCTAGACGCCGCCGTTGTGTTTTAACAGATGGTTTTTCGATTC
GTTAAGAGGAGGCAAAGTTA

Downstream 100 bases:

>100_bases
ATTTTACGTAAAAAAGGAGGATAAACTATGTCAGCATATTTGGCGGAATTTATTGGTACGATGGTTTTGATTATGTTTGG
GAATGGCCTTTTAGCAGGAT

Product: propanol dehydrogenase pduQ, putative

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MNSFQIKTKVAFGTNSLQVLKEIKNKNVWIICDRFLADGEGLQGLIGQLDVSNNVHIFTDVVPDPPISKVASGVSEAGKI
QPQVMIAFGGGSAIDTAKGIYYFAKRLEKINISTFIAIPTTSGTGSEVTAATVITDPTTKIKYPLFLDELIPDMAILDAQ
LVVTVPPAITANTGMDVLTHAIEAYVSKAANDYTDALGEKSVQLTLGFLTSCYDDGRNLANREKMHNASTMAGMAFNCAN
LGLNHSIAHQLGAQFHVPHGLANAILLDAVIRFNAFKNRDTEQKYAEMARICGIASRSDSNETAVRLLRERIVQMMEHMQ
MPRTLTDAGVAKEKVYAKMDEIATNALKDACLPTSPTTPSHQELKEILEQII

Sequences:

>Translated_372_residues
MNSFQIKTKVAFGTNSLQVLKEIKNKNVWIICDRFLADGEGLQGLIGQLDVSNNVHIFTDVVPDPPISKVASGVSEAGKI
QPQVMIAFGGGSAIDTAKGIYYFAKRLEKINISTFIAIPTTSGTGSEVTAATVITDPTTKIKYPLFLDELIPDMAILDAQ
LVVTVPPAITANTGMDVLTHAIEAYVSKAANDYTDALGEKSVQLTLGFLTSCYDDGRNLANREKMHNASTMAGMAFNCAN
LGLNHSIAHQLGAQFHVPHGLANAILLDAVIRFNAFKNRDTEQKYAEMARICGIASRSDSNETAVRLLRERIVQMMEHMQ
MPRTLTDAGVAKEKVYAKMDEIATNALKDACLPTSPTTPSHQELKEILEQII
>Mature_372_residues
MNSFQIKTKVAFGTNSLQVLKEIKNKNVWIICDRFLADGEGLQGLIGQLDVSNNVHIFTDVVPDPPISKVASGVSEAGKI
QPQVMIAFGGGSAIDTAKGIYYFAKRLEKINISTFIAIPTTSGTGSEVTAATVITDPTTKIKYPLFLDELIPDMAILDAQ
LVVTVPPAITANTGMDVLTHAIEAYVSKAANDYTDALGEKSVQLTLGFLTSCYDDGRNLANREKMHNASTMAGMAFNCAN
LGLNHSIAHQLGAQFHVPHGLANAILLDAVIRFNAFKNRDTEQKYAEMARICGIASRSDSNETAVRLLRERIVQMMEHMQ
MPRTLTDAGVAKEKVYAKMDEIATNALKDACLPTSPTTPSHQELKEILEQII

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=369, Percent_Identity=23.8482384823848, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1787493, Length=383, Percent_Identity=36.5535248041775, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI87082107, Length=359, Percent_Identity=36.2116991643454, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI48994951, Length=367, Percent_Identity=34.0599455040872, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1789163, Length=384, Percent_Identity=32.2916666666667, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1789386, Length=377, Percent_Identity=25.1989389920424, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17537053, Length=379, Percent_Identity=24.0105540897098, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321181, Length=378, Percent_Identity=34.9206349206349, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24657991, Length=373, Percent_Identity=24.1286863270777, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 40399; Mature: 40399

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSFQIKTKVAFGTNSLQVLKEIKNKNVWIICDRFLADGEGLQGLIGQLDVSNNVHIFTD
CCCEEEEEEEEECCCHHHHHHHHHCCCEEEEEHHHHCCCCCHHHHHHHEECCCCEEEEEE
VVPDPPISKVASGVSEAGKIQPQVMIAFGGGSAIDTAKGIYYFAKRLEKINISTFIAIPT
CCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEC
TSGTGSEVTAATVITDPTTKIKYPLFLDELIPDMAILDAQLVVTVPPAITANTGMDVLTH
CCCCCCCEEEEEEEECCCCCEECCHHHHHHCCCHHHHCCEEEEEECCCCCCCCCHHHHHH
AIEAYVSKAANDYTDALGEKSVQLTLGFLTSCYDDGRNLANREKMHNASTMAGMAFNCAN
HHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
LGLNHSIAHQLGAQFHVPHGLANAILLDAVIRFNAFKNRDTEQKYAEMARICGIASRSDS
CCCCHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
NETAVRLLRERIVQMMEHMQMPRTLTDAGVAKEKVYAKMDEIATNALKDACLPTSPTTPS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
HQELKEILEQII
HHHHHHHHHHHC
>Mature Secondary Structure
MNSFQIKTKVAFGTNSLQVLKEIKNKNVWIICDRFLADGEGLQGLIGQLDVSNNVHIFTD
CCCEEEEEEEEECCCHHHHHHHHHCCCEEEEEHHHHCCCCCHHHHHHHEECCCCEEEEEE
VVPDPPISKVASGVSEAGKIQPQVMIAFGGGSAIDTAKGIYYFAKRLEKINISTFIAIPT
CCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEC
TSGTGSEVTAATVITDPTTKIKYPLFLDELIPDMAILDAQLVVTVPPAITANTGMDVLTH
CCCCCCCEEEEEEEECCCCCEECCHHHHHHCCCHHHHCCEEEEEECCCCCCCCCHHHHHH
AIEAYVSKAANDYTDALGEKSVQLTLGFLTSCYDDGRNLANREKMHNASTMAGMAFNCAN
HHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
LGLNHSIAHQLGAQFHVPHGLANAILLDAVIRFNAFKNRDTEQKYAEMARICGIASRSDS
CCCCHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
NETAVRLLRERIVQMMEHMQMPRTLTDAGVAKEKVYAKMDEIATNALKDACLPTSPTTPS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
HQELKEILEQII
HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]