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The map label for this gene is pduD [H]

Identifier: 116872528

GI number: 116872528

Start: 1127183

End: 1127842

Strand: Direct

Name: pduD [H]

Synonym: lwe1112

Alternate gene names: 116872528

Gene position: 1127183-1127842 (Clockwise)

Preceding gene: 116872527

Following gene: 116872529

Centisome position: 40.05

GC content: 38.18

Gene sequence:

>660_bases
ATGGTTGAAATTAATGAAAAAGTGCTTCGTGGAATTATTTCCGAAGTATTAGATGAATTACAGCTAAAAGAAGATAAAGT
TTCTTTTCAAAAAGAACCGTCTAACCCCGTAGTTTCAGATGAAAGCTTTTTAACAGAAGTGGGGGATGCAAAGCCGGGTA
GACAAAAAGATGAAGTTGTTATCGCGGTCGCGCCTGCTTTTGGTAAATATCAAACAAAAAATATTGTTGGGGTTCCACAC
AAGCAAATTTTGCGTGAAGTGATTGCTGGAATTGAAGAAGAAGGATTAAAAGCACGCGTTGTTCGTGTTTTCCGTTCTTC
TGATGTTGCTTTCGTAGCAGTAGAAGGAGATAGATTAAGCGGTTCCGGTATTTGTATTGGTATACAGTCACGTGGCACTG
CACTAATACACCAAAAAGACTTACAACCACTTTCTAACTTAGAACTATTCCCACAAGCACCACTTATTACACTGGAAACC
TACCGTGCAATTGGTAAAAATGCAGCCAAATATGCCAAGGGTGAATCACCAAATCCAGTGCCAATGGTAAATGATCAAAT
GGCACGTCCGAAATTCCAAGCAAAAGCAGCTTTACTTCATATCAAAGAAACAAAACATGTTGTTCAAGGTAAAAATGCAG
TTGAATTACAAGTAAACTAA

Upstream 100 bases:

>100_bases
GGCGAACGTTGGGAAAAATTAAAAAATATCGCCGTTGCAGTTGATGCAAACGAATTAGATTAAGTTACTTCCCATTTTTT
ATAAAGGAGGATTAAGAGAC

Downstream 100 bases:

>100_bases
CAGTGAGGTGAGAACGATGAATCAAGAAGCTTTAGAAAATATGGTTAGAAATATTTTACAAGAAGTTAATAGTGGTGCAG
TATCCACAACTACTTCGCAA

Product: propanediol utilization dehydratase, medium subunit

Products: NA

Alternate protein names: Propanediol utilization protein pduD [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MVEINEKVLRGIISEVLDELQLKEDKVSFQKEPSNPVVSDESFLTEVGDAKPGRQKDEVVIAVAPAFGKYQTKNIVGVPH
KQILREVIAGIEEEGLKARVVRVFRSSDVAFVAVEGDRLSGSGICIGIQSRGTALIHQKDLQPLSNLELFPQAPLITLET
YRAIGKNAAKYAKGESPNPVPMVNDQMARPKFQAKAALLHIKETKHVVQGKNAVELQVN

Sequences:

>Translated_219_residues
MVEINEKVLRGIISEVLDELQLKEDKVSFQKEPSNPVVSDESFLTEVGDAKPGRQKDEVVIAVAPAFGKYQTKNIVGVPH
KQILREVIAGIEEEGLKARVVRVFRSSDVAFVAVEGDRLSGSGICIGIQSRGTALIHQKDLQPLSNLELFPQAPLITLET
YRAIGKNAAKYAKGESPNPVPMVNDQMARPKFQAKAALLHIKETKHVVQGKNAVELQVN
>Mature_219_residues
MVEINEKVLRGIISEVLDELQLKEDKVSFQKEPSNPVVSDESFLTEVGDAKPGRQKDEVVIAVAPAFGKYQTKNIVGVPH
KQILREVIAGIEEEGLKARVVRVFRSSDVAFVAVEGDRLSGSGICIGIQSRGTALIHQKDLQPLSNLELFPQAPLITLET
YRAIGKNAAKYAKGESPNPVPMVNDQMARPKFQAKAALLHIKETKHVVQGKNAVELQVN

Specific function: Part of the pduCDE complex that catalyzes the dehydration of 1,2-propanediol to propionaldehyde. Is required for S.typhimurium growth on 1,2-propanediol as the sole carbon and energy source [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diol/glycerol dehydratase medium subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010254
- InterPro:   IPR003208 [H]

Pfam domain/function: PF02288 Dehydratase_MU [H]

EC number: =4.2.1.28 [H]

Molecular weight: Translated: 23998; Mature: 23998

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEINEKVLRGIISEVLDELQLKEDKVSFQKEPSNPVVSDESFLTEVGDAKPGRQKDEVV
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEE
IAVAPAFGKYQTKNIVGVPHKQILREVIAGIEEEGLKARVVRVFRSSDVAFVAVEGDRLS
EEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEECCEEC
GSGICIGIQSRGTALIHQKDLQPLSNLELFPQAPLITLETYRAIGKNAAKYAKGESPNPV
CCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHCCHHHHHCCCCCCCCC
PMVNDQMARPKFQAKAALLHIKETKHVVQGKNAVELQVN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
>Mature Secondary Structure
MVEINEKVLRGIISEVLDELQLKEDKVSFQKEPSNPVVSDESFLTEVGDAKPGRQKDEVV
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEE
IAVAPAFGKYQTKNIVGVPHKQILREVIAGIEEEGLKARVVRVFRSSDVAFVAVEGDRLS
EEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEECCEEC
GSGICIGIQSRGTALIHQKDLQPLSNLELFPQAPLITLETYRAIGKNAAKYAKGESPNPV
CCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHCCHHHHHCCCCCCCCC
PMVNDQMARPKFQAKAALLHIKETKHVVQGKNAVELQVN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9352910; 10498708; 11677609 [H]