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The map label for this gene is pduE [H]

Identifier: 116872529

GI number: 116872529

Start: 1127859

End: 1128371

Strand: Direct

Name: pduE [H]

Synonym: lwe1113

Alternate gene names: 116872529

Gene position: 1127859-1128371 (Clockwise)

Preceding gene: 116872528

Following gene: 116872530

Centisome position: 40.08

GC content: 35.28

Gene sequence:

>513_bases
ATGAATCAAGAAGCTTTAGAAAATATGGTTAGAAATATTTTACAAGAAGTTAATAGTGGTGCAGTATCCACAACTACTTC
GCAAAAAGTAAGCGGAGATACACTAACAGTTCGTGATTATCCACTTGGTAAAAAACGTCCTGAACTTGTAAAAACTTCCA
CATCAAAATCTTTAGATGATATTACTTTAAAAAGTGTTTTAGATGGCACTATTAAACCAGAAGATGTTCGTGTAACAGCA
GAAACGCTTAAAATGCAAGCACAAGTGGCCAGAGATGCAGGTCGTGCAACACTTGCGAATAACTTCGAACGTGCGGCGGA
ATTAACTATTGTTCCAGATGAACGTATTTTAGAAATTTATAATGCAATGCGTCCATATCGTTCATCAAAAGAAGAATTAC
TTGCGATTGCAGATGAGTTGGAATCAGTTTATCATGCAACAATTTGCTCGAATTATGTTCGTGAAGCAGCACAACTTTAT
CAAGAACGTAAGAAATTAAAAGGCGATAATTAA

Upstream 100 bases:

>100_bases
CAAGCAAAAGCAGCTTTACTTCATATCAAAGAAACAAAACATGTTGTTCAAGGTAAAAATGCAGTTGAATTACAAGTAAA
CTAACAGTGAGGTGAGAACG

Downstream 100 bases:

>100_bases
TTTCAACTTTGCTCGCGATTATGTAGGAAATGCGGTGAAATGAATGAAGTATATTGCAGGTATTGATATCGGTAACTCAA
CAACGGAAGTCGCACTGGCG

Product: propanediol utilization dehydratase, small subunit

Products: NA

Alternate protein names: Propanediol utilization protein pduE [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MNQEALENMVRNILQEVNSGAVSTTTSQKVSGDTLTVRDYPLGKKRPELVKTSTSKSLDDITLKSVLDGTIKPEDVRVTA
ETLKMQAQVARDAGRATLANNFERAAELTIVPDERILEIYNAMRPYRSSKEELLAIADELESVYHATICSNYVREAAQLY
QERKKLKGDN

Sequences:

>Translated_170_residues
MNQEALENMVRNILQEVNSGAVSTTTSQKVSGDTLTVRDYPLGKKRPELVKTSTSKSLDDITLKSVLDGTIKPEDVRVTA
ETLKMQAQVARDAGRATLANNFERAAELTIVPDERILEIYNAMRPYRSSKEELLAIADELESVYHATICSNYVREAAQLY
QERKKLKGDN
>Mature_170_residues
MNQEALENMVRNILQEVNSGAVSTTTSQKVSGDTLTVRDYPLGKKRPELVKTSTSKSLDDITLKSVLDGTIKPEDVRVTA
ETLKMQAQVARDAGRATLANNFERAAELTIVPDERILEIYNAMRPYRSSKEELLAIADELESVYHATICSNYVREAAQLY
QERKKLKGDN

Specific function: Part of the pduCDE complex that catalyzes the dehydration of 1,2-propanediol to propionaldehyde. Is required for S.typhimurium growth on 1,2-propanediol as the sole carbon and energy source [H]

COG id: COG4910

COG function: function code Q; Propanediol dehydratase, small subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diol/glycerol dehydratase small subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003207 [H]

Pfam domain/function: PF02287 Dehydratase_SU [H]

EC number: =4.2.1.28 [H]

Molecular weight: Translated: 19006; Mature: 19006

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQEALENMVRNILQEVNSGAVSTTTSQKVSGDTLTVRDYPLGKKRPELVKTSTSKSLDD
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCHHH
ITLKSVLDGTIKPEDVRVTAETLKMQAQVARDAGRATLANNFERAAELTIVPDERILEIY
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEECCHHHHHHHH
NAMRPYRSSKEELLAIADELESVYHATICSNYVREAAQLYQERKKLKGDN
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNQEALENMVRNILQEVNSGAVSTTTSQKVSGDTLTVRDYPLGKKRPELVKTSTSKSLDD
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCHHH
ITLKSVLDGTIKPEDVRVTAETLKMQAQVARDAGRATLANNFERAAELTIVPDERILEIY
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEECCHHHHHHHH
NAMRPYRSSKEELLAIADELESVYHATICSNYVREAAQLYQERKKLKGDN
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9352910; 10498708; 11677609 [H]