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The map label for this gene is potD-A [H]

Identifier: 116872186

GI number: 116872186

Start: 793664

End: 794737

Strand: Direct

Name: potD-A [H]

Synonym: lwe0766

Alternate gene names: 116872186

Gene position: 793664-794737 (Clockwise)

Preceding gene: 116872185

Following gene: 116872187

Centisome position: 28.2

GC content: 37.8

Gene sequence:

>1074_bases
ATGAAGCAACTGCTGAAAATTTTTGTGCCAGTAATTGTGGTCGCGCTCCTAATGATGCTACTTGCAACAACGATGAATCG
CTCAGAAGGCTACGCTGGGAGTAATACGTTGACGATTTATAACTGGGGCGATTATATTGACCCTTCACTTATCACTAAAT
TCGAAAAAGAAACAGGTATCAAAGTCATTTACCAAACATTTGATTCTAACGAAGCGATGATGACGAAAATTGAGCAAGGT
GGAACAACTTTTGATATTGCTGTGCCAAGTGATTATGCGATTAGTAAAATGAAAGAAGAAAATTTACTAATACCGCTTGA
TCATTCCAAACTACCAAATGAAAAATACCTTGATCCTCGTTTTATGGATTTGTCGTTTGATGATAATAATAAATATTCCA
TGCCGTATTTCTGGGGGACGCTTGGGATTATATATAATAAAAAAATGTTTCCTGATAAAAAATTTGATACGTGGAATGCA
CTATTTGATCCTGAGTTGAAAAATCAAATTTTGCTTATTGACGGTGCACGTGAGGTGATGGGTCTTGGTCTAAACAGTCT
TGGTTACTCACTGAATGATACAAATAAAAACCATCTCAAAGCTGCCAGAGACAAGCTAGAGACGATGACGCCGAATGTCA
AAGCAATTGTTGGTGATGAAATCAAACTGCTCATGGCGGACAATGAAGCCGGTGTCGCGGTTACTTTCTCTGGAGAGGCA
GCAGAAATGCTCAGTGAAAATGAAGATTTAGAATATGTCATCCCAACGGACGGTTCCAATTTATGGTTCGATAACATGGT
CATTCCAAAAACAGCGAAAAATGTCGATGGCGCGCATAAATTTATTAACTTCATGTTAAAACCAGAGAATGCCGCCATCA
ATGCCGAATATGTCGGTTATGCCACACCAAACGAAAAAGCCGTCAAATTGTTACCAAAAGAAATTTCGAGTGACGAACGT
TTTTACCCGGATATGGACGAACTAAATAATTTAGAAGTATATGATAACCTCGGCAAACGAATGCTGTCGTATTATAATGA
ATTATTTTTGGAGTTTAAGATGTATCGTAAATAG

Upstream 100 bases:

>100_bases
CCACATTGATTTTCCTATTTACCATTATTTTAGTGATTGGTTATTACTTTATCAACCAACGTAATACTAGTAAAAATATT
CGGACGGGGGCGACGAAAGA

Downstream 100 bases:

>100_bases
GGTGAAAACTCAGTTAAAATGGCGTTAAATCAATAAAAAAATAGAGAAATGAGCCACCATTTTAGCTGTCAGTTTTCCAA
TTTTCTACCAAAAGCGACGA

Product: ABC transporter spermidine/putrescine binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKQLLKIFVPVIVVALLMMLLATTMNRSEGYAGSNTLTIYNWGDYIDPSLITKFEKETGIKVIYQTFDSNEAMMTKIEQG
GTTFDIAVPSDYAISKMKEENLLIPLDHSKLPNEKYLDPRFMDLSFDDNNKYSMPYFWGTLGIIYNKKMFPDKKFDTWNA
LFDPELKNQILLIDGAREVMGLGLNSLGYSLNDTNKNHLKAARDKLETMTPNVKAIVGDEIKLLMADNEAGVAVTFSGEA
AEMLSENEDLEYVIPTDGSNLWFDNMVIPKTAKNVDGAHKFINFMLKPENAAINAEYVGYATPNEKAVKLLPKEISSDER
FYPDMDELNNLEVYDNLGKRMLSYYNELFLEFKMYRK

Sequences:

>Translated_357_residues
MKQLLKIFVPVIVVALLMMLLATTMNRSEGYAGSNTLTIYNWGDYIDPSLITKFEKETGIKVIYQTFDSNEAMMTKIEQG
GTTFDIAVPSDYAISKMKEENLLIPLDHSKLPNEKYLDPRFMDLSFDDNNKYSMPYFWGTLGIIYNKKMFPDKKFDTWNA
LFDPELKNQILLIDGAREVMGLGLNSLGYSLNDTNKNHLKAARDKLETMTPNVKAIVGDEIKLLMADNEAGVAVTFSGEA
AEMLSENEDLEYVIPTDGSNLWFDNMVIPKTAKNVDGAHKFINFMLKPENAAINAEYVGYATPNEKAVKLLPKEISSDER
FYPDMDELNNLEVYDNLGKRMLSYYNELFLEFKMYRK
>Mature_357_residues
MKQLLKIFVPVIVVALLMMLLATTMNRSEGYAGSNTLTIYNWGDYIDPSLITKFEKETGIKVIYQTFDSNEAMMTKIEQG
GTTFDIAVPSDYAISKMKEENLLIPLDHSKLPNEKYLDPRFMDLSFDDNNKYSMPYFWGTLGIIYNKKMFPDKKFDTWNA
LFDPELKNQILLIDGAREVMGLGLNSLGYSLNDTNKNHLKAARDKLETMTPNVKAIVGDEIKLLMADNEAGVAVTFSGEA
AEMLSENEDLEYVIPTDGSNLWFDNMVIPKTAKNVDGAHKFINFMLKPENAAINAEYVGYATPNEKAVKLLPKEISSDER
FYPDMDELNNLEVYDNLGKRMLSYYNELFLEFKMYRK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=324, Percent_Identity=39.1975308641975, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1787078, Length=357, Percent_Identity=33.6134453781513, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1787711, Length=329, Percent_Identity=25.2279635258359, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40735; Mature: 40735

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLLKIFVPVIVVALLMMLLATTMNRSEGYAGSNTLTIYNWGDYIDPSLITKFEKETGI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCE
KVIYQTFDSNEAMMTKIEQGGTTFDIAVPSDYAISKMKEENLLIPLDHSKLPNEKYLDPR
EEEEEECCCCCHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCE
FMDLSFDDNNKYSMPYFWGTLGIIYNKKMFPDKKFDTWNALFDPELKNQILLIDGAREVM
EEEEEECCCCCEECCCHHHHHEEEECCCCCCCCCCCCCCCEECCCCCCEEEEEECCHHHH
GLGLNSLGYSLNDTNKNHLKAARDKLETMTPNVKAIVGDEIKLLMADNEAGVAVTFSGEA
HCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCEEEEECCHH
AEMLSENEDLEYVIPTDGSNLWFDNMVIPKTAKNVDGAHKFINFMLKPENAAINAEYVGY
HHHHCCCCCCEEEEECCCCCEEECCEECCCCCCCCCHHHHHHHEEECCCCCEECEEEEEE
ATPNEKAVKLLPKEISSDERFYPDMDELNNLEVYDNLGKRMLSYYNELFLEFKMYRK
CCCCCHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQLLKIFVPVIVVALLMMLLATTMNRSEGYAGSNTLTIYNWGDYIDPSLITKFEKETGI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCE
KVIYQTFDSNEAMMTKIEQGGTTFDIAVPSDYAISKMKEENLLIPLDHSKLPNEKYLDPR
EEEEEECCCCCHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCE
FMDLSFDDNNKYSMPYFWGTLGIIYNKKMFPDKKFDTWNALFDPELKNQILLIDGAREVM
EEEEEECCCCCEECCCHHHHHEEEECCCCCCCCCCCCCCCEECCCCCCEEEEEECCHHHH
GLGLNSLGYSLNDTNKNHLKAARDKLETMTPNVKAIVGDEIKLLMADNEAGVAVTFSGEA
HCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCEEEEECCHH
AEMLSENEDLEYVIPTDGSNLWFDNMVIPKTAKNVDGAHKFINFMLKPENAAINAEYVGY
HHHHCCCCCCEEEEECCCCCEEECCEECCCCCCCCCHHHHHHHEEECCCCCEECEEEEEE
ATPNEKAVKLLPKEISSDERFYPDMDELNNLEVYDNLGKRMLSYYNELFLEFKMYRK
CCCCCHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]