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The map label for this gene is flhF [H]

Identifier: 116872070

GI number: 116872070

Start: 680645

End: 681868

Strand: Direct

Name: flhF [H]

Synonym: lwe0650

Alternate gene names: 116872070

Gene position: 680645-681868 (Clockwise)

Preceding gene: 116872069

Following gene: 116872071

Centisome position: 24.19

GC content: 40.28

Gene sequence:

>1224_bases
ATGGGAAAACATTTAGTAGAAAAGATGGAAATTTTTAAGGCGAATTCCAAGCGAGAAATACATAAAAAAATTCGCTTAGT
AACGAATGAACCTTATAAAATTACGGATGAGCGTGTCACAAAGCTTGGGGTTTTTAAGAAACAGTATGAAGTGACGGCGG
TCATTATGAGTGAAGTGGCAATAGCAGATGGTCGAATAGACTTCCAAGAAACTTTTCAAAAATCAGTGGTGAAAAGTAGA
CCGAAAACAGATGATTTATTAAAAAAAGAAAAGTTACTAGAAATGCTTGCTTCGGGGGCAGAACTCGTAGCATCGACTCC
ACTGCTTGAAGAACGAAAAACACAGGAAGAAGAACTTGCAAACATGCGTCTGGAACTGGCGGCATTAAACCGAGAACTTG
CCGTGAAAATGCGTGAGGAACGTGAGCAAAATAGTGATTTTGTGAAATTTTTGAAGGGGCGCGGGATAAGCGATACTTAT
GTGGCGGACTTTATGCAGGCGGGGCGGAAACAGTTTAAACAGGTGGAAACAGCGCATTTAGACGATATCAGAGATTGGTT
CGTTCCCTATTTATCTGGAAAATTAGCAGTCGAAGATTCATTTGATTTAAGTAATCACCGGATTATTTCTCTGATTGGCC
AGACAGGTGTTGGTAAAACTACGACGCTTGTAAAACTTGGTTGGCAACTTTTAAAACAGAATCGCACAGTGGGTTTTATC
ACAACGGATACTTTTCGTTCAGGTGCGGTTGAACAGTTTCAAGGATATGCAGACAAACTTGATGTGGAGCTTATTGTTGC
GACTAGCCCAGCAGAACTTGAGGAAGCCGTGCAGTATATGACTTATGTTAGTGGAGTTGATCATATTTTGATTGATACGG
TTGGCAGGAACTATTTGGCAGAGGAATCGGTGAATGAGATTGCGGCATATACGAATGTCGTTCATCCAGATTTAACTTGT
TTTACATTTTCTTCTGGCATGAAAAGTGCTGATGTGATGACGATTTTACCAAAACTAGCAGAAATTCCAATCGATGGTTT
TATTATTACAAAAATGGATGAAACGACCCGGATTGGTGATTTATACACGGTGATGCAAGAAACGAATTTGCCGGTGCTCT
ATATGACTGACGGGCAAAATATAACTGAAAATATTTTCAGACCAAAAAGTCGTTGGTTGGCGGAACGATTTGTTGGAACG
GATAGGAGGCAGGTACTGGAATGA

Upstream 100 bases:

>100_bases
AGATGTGCCGGTGCTCTCTATTAGTGAACTGTCACCGGAAACAGAAGTGGAACAAATTGCGTTGATTGAACCAACTTGAT
GAGGGAGGTAATCCGTAGAA

Downstream 100 bases:

>100_bases
AAGGATTATATATTGGGGCTGCTGGGATGATGAATTATATGCAACATATTCAAGTTCATTCGAATAACGTGGCAAATGCG
CAGACGCCAGGTTTTAAATT

Product: flagellar biosynthesis regulator FlhF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MGKHLVEKMEIFKANSKREIHKKIRLVTNEPYKITDERVTKLGVFKKQYEVTAVIMSEVAIADGRIDFQETFQKSVVKSR
PKTDDLLKKEKLLEMLASGAELVASTPLLEERKTQEEELANMRLELAALNRELAVKMREEREQNSDFVKFLKGRGISDTY
VADFMQAGRKQFKQVETAHLDDIRDWFVPYLSGKLAVEDSFDLSNHRIISLIGQTGVGKTTTLVKLGWQLLKQNRTVGFI
TTDTFRSGAVEQFQGYADKLDVELIVATSPAELEEAVQYMTYVSGVDHILIDTVGRNYLAEESVNEIAAYTNVVHPDLTC
FTFSSGMKSADVMTILPKLAEIPIDGFIITKMDETTRIGDLYTVMQETNLPVLYMTDGQNITENIFRPKSRWLAERFVGT
DRRQVLE

Sequences:

>Translated_407_residues
MGKHLVEKMEIFKANSKREIHKKIRLVTNEPYKITDERVTKLGVFKKQYEVTAVIMSEVAIADGRIDFQETFQKSVVKSR
PKTDDLLKKEKLLEMLASGAELVASTPLLEERKTQEEELANMRLELAALNRELAVKMREEREQNSDFVKFLKGRGISDTY
VADFMQAGRKQFKQVETAHLDDIRDWFVPYLSGKLAVEDSFDLSNHRIISLIGQTGVGKTTTLVKLGWQLLKQNRTVGFI
TTDTFRSGAVEQFQGYADKLDVELIVATSPAELEEAVQYMTYVSGVDHILIDTVGRNYLAEESVNEIAAYTNVVHPDLTC
FTFSSGMKSADVMTILPKLAEIPIDGFIITKMDETTRIGDLYTVMQETNLPVLYMTDGQNITENIFRPKSRWLAERFVGT
DRRQVLE
>Mature_406_residues
GKHLVEKMEIFKANSKREIHKKIRLVTNEPYKITDERVTKLGVFKKQYEVTAVIMSEVAIADGRIDFQETFQKSVVKSRP
KTDDLLKKEKLLEMLASGAELVASTPLLEERKTQEEELANMRLELAALNRELAVKMREEREQNSDFVKFLKGRGISDTYV
ADFMQAGRKQFKQVETAHLDDIRDWFVPYLSGKLAVEDSFDLSNHRIISLIGQTGVGKTTTLVKLGWQLLKQNRTVGFIT
TDTFRSGAVEQFQGYADKLDVELIVATSPAELEEAVQYMTYVSGVDHILIDTVGRNYLAEESVNEIAAYTNVVHPDLTCF
TFSSGMKSADVMTILPKLAEIPIDGFIITKMDETTRIGDLYTVMQETNLPVLYMTDGQNITENIFRPKSRWLAERFVGTD
RRQVLE

Specific function: Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum [H]

COG id: COG1419

COG function: function code N; Flagellar GTP-binding protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding SRP family [H]

Homologues:

Organism=Escherichia coli, GI1789874, Length=193, Percent_Identity=27.979274611399, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788963, Length=199, Percent_Identity=26.6331658291457, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17539958, Length=188, Percent_Identity=27.6595744680851, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325345, Length=187, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17647949, Length=179, Percent_Identity=26.8156424581006, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000897
- InterPro:   IPR020006 [H]

Pfam domain/function: PF00448 SRP54 [H]

EC number: NA

Molecular weight: Translated: 46300; Mature: 46169

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKHLVEKMEIFKANSKREIHKKIRLVTNEPYKITDERVTKLGVFKKQYEVTAVIMSEVA
CCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IADGRIDFQETFQKSVVKSRPKTDDLLKKEKLLEMLASGAELVASTPLLEERKTQEEELA
HCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHH
NMRLELAALNRELAVKMREEREQNSDFVKFLKGRGISDTYVADFMQAGRKQFKQVETAHL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DDIRDWFVPYLSGKLAVEDSFDLSNHRIISLIGQTGVGKTTTLVKLGWQLLKQNRTVGFI
HHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEE
TTDTFRSGAVEQFQGYADKLDVELIVATSPAELEEAVQYMTYVSGVDHILIDTVGRNYLA
ECCCCCCCCHHHHHCHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHH
EESVNEIAAYTNVVHPDLTCFTFSSGMKSADVMTILPKLAEIPIDGFIITKMDETTRIGD
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
LYTVMQETNLPVLYMTDGQNITENIFRPKSRWLAERFVGTDRRQVLE
HHHHHHHCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
GKHLVEKMEIFKANSKREIHKKIRLVTNEPYKITDERVTKLGVFKKQYEVTAVIMSEVA
CHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IADGRIDFQETFQKSVVKSRPKTDDLLKKEKLLEMLASGAELVASTPLLEERKTQEEELA
HCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHH
NMRLELAALNRELAVKMREEREQNSDFVKFLKGRGISDTYVADFMQAGRKQFKQVETAHL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DDIRDWFVPYLSGKLAVEDSFDLSNHRIISLIGQTGVGKTTTLVKLGWQLLKQNRTVGFI
HHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEE
TTDTFRSGAVEQFQGYADKLDVELIVATSPAELEEAVQYMTYVSGVDHILIDTVGRNYLA
ECCCCCCCCHHHHHCHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHH
EESVNEIAAYTNVVHPDLTCFTFSSGMKSADVMTILPKLAEIPIDGFIITKMDETTRIGD
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
LYTVMQETNLPVLYMTDGQNITENIFRPKSRWLAERFVGTDRRQVLE
HHHHHHHCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1447978; 9384377 [H]