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The map label for this gene is fruA [H]

Identifier: 116872018

GI number: 116872018

Start: 635193

End: 635645

Strand: Direct

Name: fruA [H]

Synonym: lwe0598

Alternate gene names: 116872018

Gene position: 635193-635645 (Clockwise)

Preceding gene: 116872017

Following gene: 116872019

Centisome position: 22.57

GC content: 35.54

Gene sequence:

>453_bases
ATGGGCGTGAAAATCGACTTCTTGTCTATTTCAAACATGGATTTAAATACTAAAGAAGAGGCGATTCTACAATTAGTTAA
TGTGTTAGAAAAAGCTGATTATTTGGCAGATAAATCCCTTTTTGAACAAGCTATATTAGAACGCGAAAGAACGTTATCCA
CATATATTGGGCACGAAATCGGCTTGCCTCATGCCCAAAGTAGTGGCGTAAAAGAACCCTGTGTCGTTGTTGGGAAATTA
AGCAAACCAGTAAAGTGGACCACAGAGAATGAATATGTTCAAATTGTGTTTTTAATTGCTGTACCAGAAGATAACCAAAA
TAATTTACATTTAAAAGTACTGTCGAACCTTGCAAGATTATTAATGCATGAAGATTTCCGAATGAAATTAAGTGAGTTAG
ACGACACTGCAACAGTGGCACTATTAAACAAAAGTGTGAAGGAGGATAACTAA

Upstream 100 bases:

>100_bases
ATGACCTATCAAACAGATTTTATTTCCAAATTAGAACAAGCAAAAAATCCAATTGATGCTGATAGAATTATAAAAAAATA
TGAAGAGGAGGTTAGATGAT

Downstream 100 bases:

>100_bases
ATGAGAACACTTAAGTTTTTGCAAAAGCATTTGATGACAGCCACTTCTTATATGATTCCATTTGTGGTAGCTGGAGGGAT
TTTATTTGCACTATCTGTTA

Product: PTS system, fructose-specific, IIA component, putative

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 150; Mature: 149

Protein sequence:

>150_residues
MGVKIDFLSISNMDLNTKEEAILQLVNVLEKADYLADKSLFEQAILERERTLSTYIGHEIGLPHAQSSGVKEPCVVVGKL
SKPVKWTTENEYVQIVFLIAVPEDNQNNLHLKVLSNLARLLMHEDFRMKLSELDDTATVALLNKSVKEDN

Sequences:

>Translated_150_residues
MGVKIDFLSISNMDLNTKEEAILQLVNVLEKADYLADKSLFEQAILERERTLSTYIGHEIGLPHAQSSGVKEPCVVVGKL
SKPVKWTTENEYVQIVFLIAVPEDNQNNLHLKVLSNLARLLMHEDFRMKLSELDDTATVALLNKSVKEDN
>Mature_149_residues
GVKIDFLSISNMDLNTKEEAILQLVNVLEKADYLADKSLFEQAILERERTLSTYIGHEIGLPHAQSSGVKEPCVVVGKLS
KPVKWTTENEYVQIVFLIAVPEDNQNNLHLKVLSNLARLLMHEDFRMKLSELDDTATVALLNKSVKEDN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI2367327, Length=144, Percent_Identity=27.0833333333333, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI1788726, Length=131, Percent_Identity=27.4809160305344, Blast_Score=67, Evalue=3e-13,
Organism=Escherichia coli, GI1786951, Length=136, Percent_Identity=27.9411764705882, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI48994992, Length=129, Percent_Identity=26.3565891472868, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 16922; Mature: 16790

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVKIDFLSISNMDLNTKEEAILQLVNVLEKADYLADKSLFEQAILERERTLSTYIGHEI
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLPHAQSSGVKEPCVVVGKLSKPVKWTTENEYVQIVFLIAVPEDNQNNLHLKVLSNLARL
CCCCCCCCCCCCCEEEEECCCCCEEECCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHH
LMHEDFRMKLSELDDTATVALLNKSVKEDN
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GVKIDFLSISNMDLNTKEEAILQLVNVLEKADYLADKSLFEQAILERERTLSTYIGHEI
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLPHAQSSGVKEPCVVVGKLSKPVKWTTENEYVQIVFLIAVPEDNQNNLHLKVLSNLARL
CCCCCCCCCCCCCEEEEECCCCCEEECCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHH
LMHEDFRMKLSELDDTATVALLNKSVKEDN
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]