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The map label for this gene is gatC [H]

Identifier: 116871769

GI number: 116871769

Start: 369029

End: 370438

Strand: Direct

Name: gatC [H]

Synonym: lwe0349

Alternate gene names: 116871769

Gene position: 369029-370438 (Clockwise)

Preceding gene: 116871768

Following gene: 116871770

Centisome position: 13.11

GC content: 39.65

Gene sequence:

>1410_bases
ATGCTAGATATGCTTTCTGGTGTAATGGATGCTTTTGGTGCAGCAGTTGTTGTACCTATCGTTTTGATTATTATCGCGCT
TTTCCTTAAAGTAGAACCGAAAAAAGCAGTGCAATCAGGGATTAATGCTGGAATCGGCTTGACTGGATTTAACATGTTAA
TCGGCGCATATGTGCCAATCGTAACGCCGGTTATAAAACGGATGATTGATACGACAGGAATTAATTTACCAGTGTTAGAT
ACGGGTTGGCAAGCGACCTCAGTTGTTGCGTATTCTACCCAAGTGGGAATGATTTTCCTAGCGCTTGGTTTACTTCTTCA
AGTCGTACTATTTCTAACAAAATGGACAGATATATTTATGCCAGCAGACTTATGGAATAACTATTCGTTCATGCTTTGGG
GTTCGATGCTCTATATTGCAACAGGGAATATGTGGTTGGCGATTATTTTAATGGTGTTATCCAATTTATATACGCTTGTT
TTTGCCGAAATCGTTGCGAAAAGATGGTCGACCTATTATGGTTATCCAAATTGTACTATTACAGCACCGCATCACATTTG
TTCGGCGCCACTGGCAATTGGGATGGACTGGGTGTTAACAAAACTGGGAGCAAATAAAATTAACTTAAAACCAGAACAAA
TTCAAGATAAAATTGGCTTTTTAGGAGAGCCGACAACACTTGGTTTAATTTTAGGAGCAATCTTAGGTTTTGCTGGGAAC
TTCACACGACTAGATACGTTAGTTGGTTGGGGCGAAATTGCTGCAGTAGCGATTGCAACAGCTGCGATCATGGCTATTTT
CCCTAAAATTGCTGGGATTTTTGCTTCTGCTTTTACCGGAATTACCGATGCATCGAAAAAAACAGCGAAAAAAGGCAGCA
AACAACGTGTTTGGTATTTAGCTATTAATGATGCTGCCGGTTACGGTGAGTCTGCAACATTAATTACAGGTATCTTGCTT
ATTCCGATTATGGTTATTATTGCGGTTGTTTTACCAGGAAATGAAGTGCTTCCAATGGTTGATTTAATCGCTATTCCATA
CATGATTGAAGTTATTATCGCCGTTTCCAATGGGAATATTTTCAAATCCCTTATTTCTGGTGCGGTTTGGTGTACAGGTG
GACTTTATATGATTACGCAAGTAGCAGGGATTTTTACAGATGTGGCAAAAGATGTTGGCGTTGCTTTACCAGTGGGAGCT
GTGTTGATTTGTAGTTTTGCAGTGATGACTAATCACTTTATGGGCGGCCTATTCCTTATCTTTATGACCCAAAATCCGAT
ATTTATTGGAATTACAATCGTTGCTTATTTCGTGCTATACGTTCTAGTCAGAAAAAACAAACCACGGATTCACGAATACT
TAGAACGCCAAGCACTAGGTGAAGAGTACCAAGTAGCAGAAACCACTTAA

Upstream 100 bases:

>100_bases
TTTTTAACTGGTATGGATGAAGAAGGCTTTATTGAAGAACTTTTGGAAACAGCAAACGGCCTAGATTTGTAATACAAAAA
TAGAAAAAGGGGTGCTGACT

Downstream 100 bases:

>100_bases
TGAACGGAATTGGAGTGAAAAAAGATGAAAATCGTTGTTGTAGGAGATGCTCTAGTGGCAAGCTCGGATTTAGAAAAAGC
CGCTCAAGAGTTAAATGTAA

Product: PTS system, galactitol-specific IIC component, putative

Products: galactitol-1-phosphate [Cytoplasm]; pyruvate [C]

Alternate protein names: EIIC-Gat; PTS system galactitol-specific EIIC component [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MLDMLSGVMDAFGAAVVVPIVLIIIALFLKVEPKKAVQSGINAGIGLTGFNMLIGAYVPIVTPVIKRMIDTTGINLPVLD
TGWQATSVVAYSTQVGMIFLALGLLLQVVLFLTKWTDIFMPADLWNNYSFMLWGSMLYIATGNMWLAIILMVLSNLYTLV
FAEIVAKRWSTYYGYPNCTITAPHHICSAPLAIGMDWVLTKLGANKINLKPEQIQDKIGFLGEPTTLGLILGAILGFAGN
FTRLDTLVGWGEIAAVAIATAAIMAIFPKIAGIFASAFTGITDASKKTAKKGSKQRVWYLAINDAAGYGESATLITGILL
IPIMVIIAVVLPGNEVLPMVDLIAIPYMIEVIIAVSNGNIFKSLISGAVWCTGGLYMITQVAGIFTDVAKDVGVALPVGA
VLICSFAVMTNHFMGGLFLIFMTQNPIFIGITIVAYFVLYVLVRKNKPRIHEYLERQALGEEYQVAETT

Sequences:

>Translated_469_residues
MLDMLSGVMDAFGAAVVVPIVLIIIALFLKVEPKKAVQSGINAGIGLTGFNMLIGAYVPIVTPVIKRMIDTTGINLPVLD
TGWQATSVVAYSTQVGMIFLALGLLLQVVLFLTKWTDIFMPADLWNNYSFMLWGSMLYIATGNMWLAIILMVLSNLYTLV
FAEIVAKRWSTYYGYPNCTITAPHHICSAPLAIGMDWVLTKLGANKINLKPEQIQDKIGFLGEPTTLGLILGAILGFAGN
FTRLDTLVGWGEIAAVAIATAAIMAIFPKIAGIFASAFTGITDASKKTAKKGSKQRVWYLAINDAAGYGESATLITGILL
IPIMVIIAVVLPGNEVLPMVDLIAIPYMIEVIIAVSNGNIFKSLISGAVWCTGGLYMITQVAGIFTDVAKDVGVALPVGA
VLICSFAVMTNHFMGGLFLIFMTQNPIFIGITIVAYFVLYVLVRKNKPRIHEYLERQALGEEYQVAETT
>Mature_469_residues
MLDMLSGVMDAFGAAVVVPIVLIIIALFLKVEPKKAVQSGINAGIGLTGFNMLIGAYVPIVTPVIKRMIDTTGINLPVLD
TGWQATSVVAYSTQVGMIFLALGLLLQVVLFLTKWTDIFMPADLWNNYSFMLWGSMLYIATGNMWLAIILMVLSNLYTLV
FAEIVAKRWSTYYGYPNCTITAPHHICSAPLAIGMDWVLTKLGANKINLKPEQIQDKIGFLGEPTTLGLILGAILGFAGN
FTRLDTLVGWGEIAAVAIATAAIMAIFPKIAGIFASAFTGITDASKKTAKKGSKQRVWYLAINDAAGYGESATLITGILL
IPIMVIIAVVLPGNEVLPMVDLIAIPYMIEVIIAVSNGNIFKSLISGAVWCTGGLYMITQVAGIFTDVAKDVGVALPVGA
VLICSFAVMTNHFMGGLFLIFMTQNPIFIGITIVAYFVLYVLVRKNKPRIHEYLERQALGEEYQVAETT

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3775

COG function: function code G; Phosphotransferase system, galactitol-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788408, Length=465, Percent_Identity=27.0967741935484, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1790758, Length=399, Percent_Identity=30.0751879699248, Blast_Score=172, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013853
- InterPro:   IPR013014
- InterPro:   IPR004703 [H]

Pfam domain/function: PF03611 EIIC-GAT [H]

EC number: NA

Molecular weight: Translated: 50636; Mature: 50636

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDMLSGVMDAFGAAVVVPIVLIIIALFLKVEPKKAVQSGINAGIGLTGFNMLIGAYVPI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VTPVIKRMIDTTGINLPVLDTGWQATSVVAYSTQVGMIFLALGLLLQVVLFLTKWTDIFM
HHHHHHHHHHHCCCCCCEECCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PADLWNNYSFMLWGSMLYIATGNMWLAIILMVLSNLYTLVFAEIVAKRWSTYYGYPNCTI
CHHHHCCCCHHHHHHEHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
TAPHHICSAPLAIGMDWVLTKLGANKINLKPEQIQDKIGFLGEPTTLGLILGAILGFAGN
ECCHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
FTRLDTLVGWGEIAAVAIATAAIMAIFPKIAGIFASAFTGITDASKKTAKKGSKQRVWYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEE
AINDAAGYGESATLITGILLIPIMVIIAVVLPGNEVLPMVDLIAIPYMIEVIIAVSNGNI
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEECCCHH
FKSLISGAVWCTGGLYMITQVAGIFTDVAKDVGVALPVGAVLICSFAVMTNHFMGGLFLI
HHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEE
FMTQNPIFIGITIVAYFVLYVLVRKNKPRIHEYLERQALGEEYQVAETT
EEECCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLDMLSGVMDAFGAAVVVPIVLIIIALFLKVEPKKAVQSGINAGIGLTGFNMLIGAYVPI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VTPVIKRMIDTTGINLPVLDTGWQATSVVAYSTQVGMIFLALGLLLQVVLFLTKWTDIFM
HHHHHHHHHHHCCCCCCEECCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PADLWNNYSFMLWGSMLYIATGNMWLAIILMVLSNLYTLVFAEIVAKRWSTYYGYPNCTI
CHHHHCCCCHHHHHHEHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
TAPHHICSAPLAIGMDWVLTKLGANKINLKPEQIQDKIGFLGEPTTLGLILGAILGFAGN
ECCHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
FTRLDTLVGWGEIAAVAIATAAIMAIFPKIAGIFASAFTGITDASKKTAKKGSKQRVWYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEE
AINDAAGYGESATLITGILLIPIMVIIAVVLPGNEVLPMVDLIAIPYMIEVIIAVSNGNI
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEECCCHH
FKSLISGAVWCTGGLYMITQVAGIFTDVAKDVGVALPVGAVLICSFAVMTNHFMGGLFLI
HHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEE
FMTQNPIFIGITIVAYFVLYVLVRKNKPRIHEYLERQALGEEYQVAETT
EEECCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoenolpyruvate; galactitol [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + galactitol [Periplasm] = galactitol-1-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]