Click here to switch to the map view.

The map label for this gene is fruA [H]

Identifier: 116871734

GI number: 116871734

Start: 339580

End: 340026

Strand: Direct

Name: fruA [H]

Synonym: lwe0314

Alternate gene names: 116871734

Gene position: 339580-340026 (Clockwise)

Preceding gene: 116871733

Following gene: 116871735

Centisome position: 12.07

GC content: 29.31

Gene sequence:

>447_bases
ATGAAGATATCCAATTTAATTAATGAAGACCGAATTATTTTTGATAATCGTATTCAGACCAAGGAATTATTATTTGAAAA
AGTGGCGGAAGTGCTAGTTAAAGAGGGTTCAATAACTAATACGAAAAAATTTATCCGCGACTTATATAAGCGAGAAGAAG
AAACTTCCACAGGAATTGAAGCAGGTTTTGGCATTCCTCATGCAAAAAGTAAATATGTAAAAGAACCATTGATTGTTTTT
GTTCATTCTGATATTATAAATGATTATTTTGGATTAGATGATTCACCAATTGAATGTAGTTTTCTGATTGGCGTTCCAAA
AAAAGCAACAGACGTTCATCTTCAAATATTAAGTGAACTATCCAGAAAGTTAATGGACGAAAAGTTTCGCGAAAAATTAA
AAAATTCCAAAAACAAAAATGAAATTATAACAATTTTATCGAACTAA

Upstream 100 bases:

>100_bases
TTTTGCATAATTAGCCCATAATATTGAATGAATTAGTTCATCGTGATATAGTGTAATCAAACAAGTGAAGCGCTTACTTT
GTTGGGAGAGGATGTGAATT

Downstream 100 bases:

>100_bases
GGAGGCGTTTCACATGAAAATAGTTGGTGTTACTGCTTGTCCCACTGGTATTGCTCATACGTATATGTCTGCCGAAAAAT
TAACAATAACGGCGGAAGCT

Product: PTS system, fructose-specific IIA component

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MKISNLINEDRIIFDNRIQTKELLFEKVAEVLVKEGSITNTKKFIRDLYKREEETSTGIEAGFGIPHAKSKYVKEPLIVF
VHSDIINDYFGLDDSPIECSFLIGVPKKATDVHLQILSELSRKLMDEKFREKLKNSKNKNEIITILSN

Sequences:

>Translated_148_residues
MKISNLINEDRIIFDNRIQTKELLFEKVAEVLVKEGSITNTKKFIRDLYKREEETSTGIEAGFGIPHAKSKYVKEPLIVF
VHSDIINDYFGLDDSPIECSFLIGVPKKATDVHLQILSELSRKLMDEKFREKLKNSKNKNEIITILSN
>Mature_148_residues
MKISNLINEDRIIFDNRIQTKELLFEKVAEVLVKEGSITNTKKFIRDLYKREEETSTGIEAGFGIPHAKSKYVKEPLIVF
VHSDIINDYFGLDDSPIECSFLIGVPKKATDVHLQILSELSRKLMDEKFREKLKNSKNKNEIITILSN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI2367327, Length=125, Percent_Identity=26.4, Blast_Score=76, Evalue=9e-16,
Organism=Escherichia coli, GI1786951, Length=150, Percent_Identity=23.3333333333333, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI48994992, Length=146, Percent_Identity=28.0821917808219, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17057; Mature: 17057

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISNLINEDRIIFDNRIQTKELLFEKVAEVLVKEGSITNTKKFIRDLYKREEETSTGIE
CCCHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
AGFGIPHAKSKYVKEPLIVFVHSDIINDYFGLDDSPIECSFLIGVPKKATDVHLQILSEL
CCCCCCCCCHHHHCCHHHEEEHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
SRKLMDEKFREKLKNSKNKNEIITILSN
HHHHHHHHHHHHHHCCCCCCEEEEEECC
>Mature Secondary Structure
MKISNLINEDRIIFDNRIQTKELLFEKVAEVLVKEGSITNTKKFIRDLYKREEETSTGIE
CCCHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
AGFGIPHAKSKYVKEPLIVFVHSDIINDYFGLDDSPIECSFLIGVPKKATDVHLQILSEL
CCCCCCCCCHHHHCCHHHEEEHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
SRKLMDEKFREKLKNSKNKNEIITILSN
HHHHHHHHHHHHHHCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]