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The map label for this gene is dapE [H]

Identifier: 116871655

GI number: 116871655

Start: 250990

End: 252129

Strand: Direct

Name: dapE [H]

Synonym: lwe0235

Alternate gene names: 116871655

Gene position: 250990-252129 (Clockwise)

Preceding gene: 116871654

Following gene: 116871656

Centisome position: 8.92

GC content: 35.53

Gene sequence:

>1140_bases
ATGGATCAACAAAAAAAGATTCAAATTTTAAAGGACATGGTAAATATTGATTCGACTAATGGGCATGAAGAACAGGTTGC
GAATTATTTACAGAAGTTGTTAGCTGAATATGGGATTGAATCTGAGAAAGTGCTGTATGATGAAGACAGGGCAAGTCTTG
TTAGTGAAATAGGTTCTAGCGATGAGCAAGTTTTGGCTTTTTCAGGGCATATGGATGTGGTTGATGCAGGAGATGTGTCA
AAATGGAAGTTTCCTCCTTTTGAAGCAACGGAGCAGGAAGGGAAAATATACGGCCGTGGTGCCACGGATATGAAGTCAGG
TTTAGCAGCGATGGTCATTGCAATGATTGAGCTGCAAGAAGAAAAGCAAAAACTAAATGGGAAAATTAGATTATTAGCTA
CAGTTGGTGAAGAAGTTGGCGAACTTGGAGCAGAACAACTAACTCAAAAAGGTTATGCAGATGACTTAGATGGTTTAATT
ATTGGGGAACCAAGTGGACACCGAATTGTATATGCGCATAAAGGTTCGATTAATTATACCGTTAAATCTACTGGTAAAAA
TGCGCATAGTTCGATGCCAGAATATGGTGTGAATGCAATTGATAATTTGATGCTATTTTATAATGAAATAGAAAAATATG
TTGCTTCCATCCACACTACAAATGAAATACTAGGTGATTTTATCCATAATGTCACAGTAATAAGTGGTGGAAACCAAGTC
AATAGTATCCCAGAAAAAGCAGAACTACAAGGTAATATCCGTTCTATTCCAGAAGTAGATAACGAAACTTTGAAACGGCG
AATTGTTGATATTGTAAATGAATTAAATAAAAAAGAACATGTACAGCTAGAGTTAATATTTGATTACGATAAACAACCTG
TATTTAGTGACAGAAATTCAAGATTAGTTCATGTTGCGCGTGATGTTGCGAAAGGTATTATCAAGGAAGAGATACCGTTA
CTTGGAATTTCTGGAACAACAGATGCAGCCGAGTTTACTAAAGCTAAACATAAGTTCCCAGTAATTATCTTCGGCCCAGG
GAATGAAACGCCACATCAAATAAATGAAAATGTCTCCATAGATAATTATTTAGAAATGGTAGATGTATATAAAAAAATTG
CAGTAGAATATTTAAGTTAA

Upstream 100 bases:

>100_bases
AGCAATAAATTTGTGAGCCTTTATTACACTTCTTTCTTTGTTTCCTCAAAAAAAATTAGGGAAAATATATGATGAATGAT
ATAGAAAGAAGGCGTAAAAT

Downstream 100 bases:

>100_bases
TTATAAAAAACCAATTTGATTTTGTGTCAAATTGGTTTTTTATTAGTTTCAATAAGTGAAAATACTTGCTTTTTAAACAG
TTTAGTACTAAACTAAAATT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MDQQKKIQILKDMVNIDSTNGHEEQVANYLQKLLAEYGIESEKVLYDEDRASLVSEIGSSDEQVLAFSGHMDVVDAGDVS
KWKFPPFEATEQEGKIYGRGATDMKSGLAAMVIAMIELQEEKQKLNGKIRLLATVGEEVGELGAEQLTQKGYADDLDGLI
IGEPSGHRIVYAHKGSINYTVKSTGKNAHSSMPEYGVNAIDNLMLFYNEIEKYVASIHTTNEILGDFIHNVTVISGGNQV
NSIPEKAELQGNIRSIPEVDNETLKRRIVDIVNELNKKEHVQLELIFDYDKQPVFSDRNSRLVHVARDVAKGIIKEEIPL
LGISGTTDAAEFTKAKHKFPVIIFGPGNETPHQINENVSIDNYLEMVDVYKKIAVEYLS

Sequences:

>Translated_379_residues
MDQQKKIQILKDMVNIDSTNGHEEQVANYLQKLLAEYGIESEKVLYDEDRASLVSEIGSSDEQVLAFSGHMDVVDAGDVS
KWKFPPFEATEQEGKIYGRGATDMKSGLAAMVIAMIELQEEKQKLNGKIRLLATVGEEVGELGAEQLTQKGYADDLDGLI
IGEPSGHRIVYAHKGSINYTVKSTGKNAHSSMPEYGVNAIDNLMLFYNEIEKYVASIHTTNEILGDFIHNVTVISGGNQV
NSIPEKAELQGNIRSIPEVDNETLKRRIVDIVNELNKKEHVQLELIFDYDKQPVFSDRNSRLVHVARDVAKGIIKEEIPL
LGISGTTDAAEFTKAKHKFPVIIFGPGNETPHQINENVSIDNYLEMVDVYKKIAVEYLS
>Mature_379_residues
MDQQKKIQILKDMVNIDSTNGHEEQVANYLQKLLAEYGIESEKVLYDEDRASLVSEIGSSDEQVLAFSGHMDVVDAGDVS
KWKFPPFEATEQEGKIYGRGATDMKSGLAAMVIAMIELQEEKQKLNGKIRLLATVGEEVGELGAEQLTQKGYADDLDGLI
IGEPSGHRIVYAHKGSINYTVKSTGKNAHSSMPEYGVNAIDNLMLFYNEIEKYVASIHTTNEILGDFIHNVTVISGGNQV
NSIPEKAELQGNIRSIPEVDNETLKRRIVDIVNELNKKEHVQLELIFDYDKQPVFSDRNSRLVHVARDVAKGIIKEEIPL
LGISGTTDAAEFTKAKHKFPVIIFGPGNETPHQINENVSIDNYLEMVDVYKKIAVEYLS

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; fifth step. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=324, Percent_Identity=29.0123456790123, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790395, Length=382, Percent_Identity=28.5340314136126, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789236, Length=407, Percent_Identity=22.3587223587224, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17551016, Length=259, Percent_Identity=26.2548262548263, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR010182
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 42179; Mature: 42179

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQQKKIQILKDMVNIDSTNGHEEQVANYLQKLLAEYGIESEKVLYDEDRASLVSEIGSS
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHCCC
DEQVLAFSGHMDVVDAGDVSKWKFPPFEATEQEGKIYGRGATDMKSGLAAMVIAMIELQE
CCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
EKQKLNGKIRLLATVGEEVGELGAEQLTQKGYADDLDGLIIGEPSGHRIVYAHKGSINYT
HHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEECCCEEEE
VKSTGKNAHSSMPEYGVNAIDNLMLFYNEIEKYVASIHTTNEILGDFIHNVTVISGGNQV
EECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCC
NSIPEKAELQGNIRSIPEVDNETLKRRIVDIVNELNKKEHVQLELIFDYDKQPVFSDRNS
CCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCC
RLVHVARDVAKGIIKEEIPLLGISGTTDAAEFTKAKHKFPVIIFGPGNETPHQINENVSI
HHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCHHCCCCCCH
DNYLEMVDVYKKIAVEYLS
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDQQKKIQILKDMVNIDSTNGHEEQVANYLQKLLAEYGIESEKVLYDEDRASLVSEIGSS
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHCCC
DEQVLAFSGHMDVVDAGDVSKWKFPPFEATEQEGKIYGRGATDMKSGLAAMVIAMIELQE
CCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
EKQKLNGKIRLLATVGEEVGELGAEQLTQKGYADDLDGLIIGEPSGHRIVYAHKGSINYT
HHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEECCCEEEE
VKSTGKNAHSSMPEYGVNAIDNLMLFYNEIEKYVASIHTTNEILGDFIHNVTVISGGNQV
EECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCC
NSIPEKAELQGNIRSIPEVDNETLKRRIVDIVNELNKKEHVQLELIFDYDKQPVFSDRNS
CCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCC
RLVHVARDVAKGIIKEEIPLLGISGTTDAAEFTKAKHKFPVIIFGPGNETPHQINENVSI
HHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCHHCCCCCCH
DNYLEMVDVYKKIAVEYLS
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]