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The map label for this gene is dus1 [H]

Identifier: 116871611

GI number: 116871611

Start: 190836

End: 191831

Strand: Direct

Name: dus1 [H]

Synonym: lwe0191

Alternate gene names: 116871611

Gene position: 190836-191831 (Clockwise)

Preceding gene: 116871610

Following gene: 116871612

Centisome position: 6.78

GC content: 39.76

Gene sequence:

>996_bases
ATGTTTAAAATAGGTAATGTTGAAATTAAGAATCAAGTAGTTGTGGCACCAATGGCCGGTATATCCAATTCCGCATTTCG
TTTAACAGTCAAAGAATTTGGCGCAGGCCTTGTTTGCTGTGAAATGATTAGTGACAAAGGAATTGCATACCGTAATGCTA
AAACGCTTGATATGCTATATATTGATGAAAAAGAAAAACCACTGAGCTTACAGATTTTCGGTGGTGAGAAAGAAACGCTT
GTAGAAGCCGCAAAATTTGTAGCTGAAAATACAACTGCGGATATTATTGACATTAATATGGGGTGCCCGGTAAATAAAAT
TATCAAATGTGAAGCTGGAGCGAAGTGGTTACTTGACCCAAATAAAGTGTATGACATGGTATCTGCTGTAGTAGATGCTG
TTGATAAACCGGTAACAGTAAAAATGCGTATTGGTTGGGATGAAGAGCATGTTTTCGCTATTGAAAACGCACTTGCAGCT
GAACGTGCAGGAGCTTCAGCTGTTGCTATGCACGGTCGTACACGTGTTCAAATGTACGAAGGCAGTGCAAACTGGGACGT
GTTAAGAGATGTTAAACGCGAACTAAAAATTCCATTCATGGCAAATGGGGATGTGAAAACACCCGAAGATGCAAAAAGAA
TCTTAGAACATACAGGTGCAGATGGCGTTATGATTGGTCGTGCTGCTCTTGGAAACCCTTGGATGATTTACCGTACTGTG
AAATTTTTAGAAACAGGAGAACTTCTACCTGAACCAGAACCACGTGAAAAAATGCAAACGGCTATGCTTCATTTGAACCG
TCTAGTTGAATTAAAAGGCGAAAACATTGCAGTTCGAGAATTCAGACAACACGCAGCATACTATCTAAAAGGAGCTCGTG
GAAGTACTCGTGCCAAAGTGGCCGCTAACCAAGCGACAAAGCAATCAGAAATGGAAGCAATTTTAAATGAATTCGTACTT
CAATATGAAGAAAAAGCATTAGCTAAACAAGATTAA

Upstream 100 bases:

>100_bases
AATAAAAAATCAAGTGAAGTGGTAAAATTGGCACTTGCAGAAAAAACTGATTATGATAACATATCACTAGCGCAATTTAA
GAGGAAGGGGTTTACCAATA

Downstream 100 bases:

>100_bases
TTATTGTTGCCTCGAGATTTCGGGGCAATTTTTAAATTTACACTAGAAAAATACGACTAAAAAAGCGTATAATGGAAACA
TCTGTAGAAATGAATAGAAT

Product: dihydrouridine synthase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MFKIGNVEIKNQVVVAPMAGISNSAFRLTVKEFGAGLVCCEMISDKGIAYRNAKTLDMLYIDEKEKPLSLQIFGGEKETL
VEAAKFVAENTTADIIDINMGCPVNKIIKCEAGAKWLLDPNKVYDMVSAVVDAVDKPVTVKMRIGWDEEHVFAIENALAA
ERAGASAVAMHGRTRVQMYEGSANWDVLRDVKRELKIPFMANGDVKTPEDAKRILEHTGADGVMIGRAALGNPWMIYRTV
KFLETGELLPEPEPREKMQTAMLHLNRLVELKGENIAVREFRQHAAYYLKGARGSTRAKVAANQATKQSEMEAILNEFVL
QYEEKALAKQD

Sequences:

>Translated_331_residues
MFKIGNVEIKNQVVVAPMAGISNSAFRLTVKEFGAGLVCCEMISDKGIAYRNAKTLDMLYIDEKEKPLSLQIFGGEKETL
VEAAKFVAENTTADIIDINMGCPVNKIIKCEAGAKWLLDPNKVYDMVSAVVDAVDKPVTVKMRIGWDEEHVFAIENALAA
ERAGASAVAMHGRTRVQMYEGSANWDVLRDVKRELKIPFMANGDVKTPEDAKRILEHTGADGVMIGRAALGNPWMIYRTV
KFLETGELLPEPEPREKMQTAMLHLNRLVELKGENIAVREFRQHAAYYLKGARGSTRAKVAANQATKQSEMEAILNEFVL
QYEEKALAKQD
>Mature_331_residues
MFKIGNVEIKNQVVVAPMAGISNSAFRLTVKEFGAGLVCCEMISDKGIAYRNAKTLDMLYIDEKEKPLSLQIFGGEKETL
VEAAKFVAENTTADIIDINMGCPVNKIIKCEAGAKWLLDPNKVYDMVSAVVDAVDKPVTVKMRIGWDEEHVFAIENALAA
ERAGASAVAMHGRTRVQMYEGSANWDVLRDVKRELKIPFMANGDVKTPEDAKRILEHTGADGVMIGRAALGNPWMIYRTV
KFLETGELLPEPEPREKMQTAMLHLNRLVELKGENIAVREFRQHAAYYLKGARGSTRAKVAANQATKQSEMEAILNEFVL
QYEEKALAKQD

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=231, Percent_Identity=29.4372294372294, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI239788462, Length=231, Percent_Identity=29.4372294372294, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI8923374, Length=247, Percent_Identity=29.5546558704453, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI31742496, Length=230, Percent_Identity=32.1739130434783, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI40807366, Length=224, Percent_Identity=29.9107142857143, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1789660, Length=291, Percent_Identity=40.2061855670103, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1788462, Length=298, Percent_Identity=29.1946308724832, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI145693211, Length=244, Percent_Identity=29.0983606557377, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=228, Percent_Identity=31.140350877193, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17507177, Length=233, Percent_Identity=31.7596566523605, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=28.3400809716599, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI25144369, Length=222, Percent_Identity=30.6306306306306, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6323560, Length=230, Percent_Identity=33.0434782608696, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323437, Length=223, Percent_Identity=27.3542600896861, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=258, Percent_Identity=25.968992248062, Blast_Score=82, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24585320, Length=240, Percent_Identity=32.5, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24580595, Length=256, Percent_Identity=32.03125, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI19920448, Length=256, Percent_Identity=32.03125, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI45549423, Length=260, Percent_Identity=31.5384615384615, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19921524, Length=316, Percent_Identity=25.6329113924051, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36779; Mature: 36779

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIGNVEIKNQVVVAPMAGISNSAFRLTVKEFGAGLVCCEMISDKGIAYRNAKTLDMLY
CCCCCCEEECCEEEEEECCCCCCCEEEEEHHHHCCCCEEEHHHHCCCCEEECCCEEEEEE
IDEKEKPLSLQIFGGEKETLVEAAKFVAENTTADIIDINMGCPVNKIIKCEAGAKWLLDP
ECCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCEEEECH
NKVYDMVSAVVDAVDKPVTVKMRIGWDEEHVFAIENALAAERAGASAVAMHGRTRVQMYE
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHHHHHCCCCEEEECCCEEEEEEE
GSANWDVLRDVKRELKIPFMANGDVKTPEDAKRILEHTGADGVMIGRAALGNPWMIYRTV
CCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCCEEEEHHHCCCCEEEEEEH
KFLETGELLPEPEPREKMQTAMLHLNRLVELKGENIAVREFRQHAAYYLKGARGSTRAKV
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEECCCCCCHHHH
AANQATKQSEMEAILNEFVLQYEEKALAKQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFKIGNVEIKNQVVVAPMAGISNSAFRLTVKEFGAGLVCCEMISDKGIAYRNAKTLDMLY
CCCCCCEEECCEEEEEECCCCCCCEEEEEHHHHCCCCEEEHHHHCCCCEEECCCEEEEEE
IDEKEKPLSLQIFGGEKETLVEAAKFVAENTTADIIDINMGCPVNKIIKCEAGAKWLLDP
ECCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCEEEECH
NKVYDMVSAVVDAVDKPVTVKMRIGWDEEHVFAIENALAAERAGASAVAMHGRTRVQMYE
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHHHHHCCCCEEEECCCEEEEEEE
GSANWDVLRDVKRELKIPFMANGDVKTPEDAKRILEHTGADGVMIGRAALGNPWMIYRTV
CCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCCEEEEHHHCCCCEEEEEEH
KFLETGELLPEPEPREKMQTAMLHLNRLVELKGENIAVREFRQHAAYYLKGARGSTRAKV
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEECCCCCCHHHH
AANQATKQSEMEAILNEFVLQYEEKALAKQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]