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The map label for this gene is lysS [H]

Identifier: 116871612

GI number: 116871612

Start: 191946

End: 193442

Strand: Direct

Name: lysS [H]

Synonym: lwe0192

Alternate gene names: 116871612

Gene position: 191946-193442 (Clockwise)

Preceding gene: 116871611

Following gene: 116871613

Centisome position: 6.82

GC content: 38.94

Gene sequence:

>1497_bases
ATGAGTAACGAGAATCATGAAGAACTAAATGACCAACTCATCGTCCGTCGGGAAAAAGTGGACACATTGCGTGAAGAAGG
CATAGATCCTTTTGGTGAAAAATTTATCCGTTCCATCAGCCCAGAAGAAATTGAAACAAAATTCGCTGATAAATCAAAAG
AAGAACTTGAGGAAGCTGCTATCGAAGTTTCTGTAGCAGGTCGTATTATGACGAAGCGTGTAAAAGGTAAAGTAGGTTTC
ACGCATATTCAAGACCGTTTCCATCAATTACAAATCTATATTCGTAAAGACGCTATTGGGGAAGATGCATATGCGATTTT
CAAATTAGCTGATTTAGGAGACATTATTGGCATAAAGGGAACTATTTTCCGTACTAATACAGGAGAATTATCAGTAAAAG
CGACTGATTTTACTTTACTTTCAAAATCATTACGCCCGCTTCCTGATAAATATCATGGCTTAAAAGACGTAGAACAGCGC
TACCGCCAACGCTATTTAGACTTAATTACAAATGAAGAAAGTCAAAATCGTTTTGTTATGCGTAGTAAAATTTTGAAATA
TACACGTGATTACATGGACAGCCAAGGTTTCTTAGAAGTAGAAACTCCAGTGCTCCATACAATTGCTGGTGGCGCTGCAG
CAAAACCATTTATAACGCACCATAATGCACTTGATATGGAACTATACTTACGAATCGCATTGGAACTACATTTGAAACGT
CTAATCGTTGGCGGGATGGATAAGGTATATGAGATTGGTCGTGTCTTCCGTAATGAAGGAACGTCCACGCGACATAACCC
TGAATTTACAATGTTAGAATCATATGCGGCTTACGAAGACTATGAAGATGTTATGGATTTAGTAGAAGGTTTAGTATCAA
CTGTTTGTAAACAAGTAAATGGAACAACAGAAATAACATATGGCGAATACAAAGTAGATTTGACTCCGAATTGGCGCCGC
GTTCACATGGCAGATGTTGTTAAAGAATACGTAGGTGTAGACTTCTGGAATGTGACTTCCGATGAAGAAGCGCGCGAACT
TGCTAAGAAACACGATGTTGCTATAACTGAACACATGACTTATGGTCACATTCTTAACGAGTTTTTCGAAACATACGTAG
AAGAAAAACTTATTCAACCTACATTTGTGTATGGTCACCCAGTAGAAATTTCTCCTCTAGCGAAGAAAAACAAAGAGGAT
GAGCGATTTACTGACCGGTTTGAATTATTCATTGTTGGCCGTGAACACGCTAATGCATTCTCAGAGTTAAATGACCCAAT
TGATCAAAGAGAACGTTTTGAAGCGCAAATGAAAGAACGTGAACAAGGAAATGATGAAGCGCACGGAATGGATGCGGATT
TCCTTGAAGCTTTAGAATATGGTCTGCCTCCAACAGGTGGATTAGGTATAGGTGTAGACCGTCTAGTAATGTTATTAACA
GATGCGCCGTCTATCCGTGATATCCTATTATTCCCAACCATGAAACACCGTGACTAA

Upstream 100 bases:

>100_bases
AGATTTCGGGGCAATTTTTAAATTTACACTAGAAAAATACGACTAAAAAAGCGTATAATGGAAACATCTGTAGAAATGAA
TAGAATAGGAGTGTACCTGT

Downstream 100 bases:

>100_bases
CAATCAATCCAGAGCTGTTTTTTACAGTTCTGGATTTTGCTATTTTTTTAGAAAAAAACAAATTTGCCAAAGAGAAACTT
TTTCTTAAAATATGCTTGCA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MSNENHEELNDQLIVRREKVDTLREEGIDPFGEKFIRSISPEEIETKFADKSKEELEEAAIEVSVAGRIMTKRVKGKVGF
THIQDRFHQLQIYIRKDAIGEDAYAIFKLADLGDIIGIKGTIFRTNTGELSVKATDFTLLSKSLRPLPDKYHGLKDVEQR
YRQRYLDLITNEESQNRFVMRSKILKYTRDYMDSQGFLEVETPVLHTIAGGAAAKPFITHHNALDMELYLRIALELHLKR
LIVGGMDKVYEIGRVFRNEGTSTRHNPEFTMLESYAAYEDYEDVMDLVEGLVSTVCKQVNGTTEITYGEYKVDLTPNWRR
VHMADVVKEYVGVDFWNVTSDEEARELAKKHDVAITEHMTYGHILNEFFETYVEEKLIQPTFVYGHPVEISPLAKKNKED
ERFTDRFELFIVGREHANAFSELNDPIDQRERFEAQMKEREQGNDEAHGMDADFLEALEYGLPPTGGLGIGVDRLVMLLT
DAPSIRDILLFPTMKHRD

Sequences:

>Translated_498_residues
MSNENHEELNDQLIVRREKVDTLREEGIDPFGEKFIRSISPEEIETKFADKSKEELEEAAIEVSVAGRIMTKRVKGKVGF
THIQDRFHQLQIYIRKDAIGEDAYAIFKLADLGDIIGIKGTIFRTNTGELSVKATDFTLLSKSLRPLPDKYHGLKDVEQR
YRQRYLDLITNEESQNRFVMRSKILKYTRDYMDSQGFLEVETPVLHTIAGGAAAKPFITHHNALDMELYLRIALELHLKR
LIVGGMDKVYEIGRVFRNEGTSTRHNPEFTMLESYAAYEDYEDVMDLVEGLVSTVCKQVNGTTEITYGEYKVDLTPNWRR
VHMADVVKEYVGVDFWNVTSDEEARELAKKHDVAITEHMTYGHILNEFFETYVEEKLIQPTFVYGHPVEISPLAKKNKED
ERFTDRFELFIVGREHANAFSELNDPIDQRERFEAQMKEREQGNDEAHGMDADFLEALEYGLPPTGGLGIGVDRLVMLLT
DAPSIRDILLFPTMKHRD
>Mature_497_residues
SNENHEELNDQLIVRREKVDTLREEGIDPFGEKFIRSISPEEIETKFADKSKEELEEAAIEVSVAGRIMTKRVKGKVGFT
HIQDRFHQLQIYIRKDAIGEDAYAIFKLADLGDIIGIKGTIFRTNTGELSVKATDFTLLSKSLRPLPDKYHGLKDVEQRY
RQRYLDLITNEESQNRFVMRSKILKYTRDYMDSQGFLEVETPVLHTIAGGAAAKPFITHHNALDMELYLRIALELHLKRL
IVGGMDKVYEIGRVFRNEGTSTRHNPEFTMLESYAAYEDYEDVMDLVEGLVSTVCKQVNGTTEITYGEYKVDLTPNWRRV
HMADVVKEYVGVDFWNVTSDEEARELAKKHDVAITEHMTYGHILNEFFETYVEEKLIQPTFVYGHPVEISPLAKKNKEDE
RFTDRFELFIVGREHANAFSELNDPIDQRERFEAQMKEREQGNDEAHGMDADFLEALEYGLPPTGGLGIGVDRLVMLLTD
APSIRDILLFPTMKHRD

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=503, Percent_Identity=43.1411530815109, Blast_Score=397, Evalue=1e-111,
Organism=Homo sapiens, GI194272210, Length=508, Percent_Identity=42.9133858267717, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI40789249, Length=242, Percent_Identity=26.0330578512397, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1790571, Length=490, Percent_Identity=50.6122448979592, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1789256, Length=490, Percent_Identity=50.4081632653061, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI87082379, Length=322, Percent_Identity=32.9192546583851, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI1788173, Length=317, Percent_Identity=26.4984227129338, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=501, Percent_Identity=46.5069860279441, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17535927, Length=501, Percent_Identity=46.5069860279441, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71994340, Length=501, Percent_Identity=46.5069860279441, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17551876, Length=472, Percent_Identity=22.8813559322034, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=172, Percent_Identity=25.5813953488372, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=494, Percent_Identity=43.7246963562753, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324256, Length=474, Percent_Identity=33.1223628691983, Blast_Score=213, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6323011, Length=358, Percent_Identity=25.9776536312849, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321807, Length=330, Percent_Identity=25.1515151515152, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24640849, Length=506, Percent_Identity=42.8853754940711, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24640851, Length=506, Percent_Identity=42.8853754940711, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17136276, Length=471, Percent_Identity=23.7791932059448, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24584738, Length=261, Percent_Identity=26.4367816091954, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57400; Mature: 57269

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNENHEELNDQLIVRREKVDTLREEGIDPFGEKFIRSISPEEIETKFADKSKEELEEAA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHH
IEVSVAGRIMTKRVKGKVGFTHIQDRFHQLQIYIRKDAIGEDAYAIFKLADLGDIIGIKG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHCCCCCCCCE
TIFRTNTGELSVKATDFTLLSKSLRPLPDKYHGLKDVEQRYRQRYLDLITNEESQNRFVM
EEEECCCCCEEEEECHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
RSKILKYTRDYMDSQGFLEVETPVLHTIAGGAAAKPFITHHNALDMELYLRIALELHLKR
HHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHH
LIVGGMDKVYEIGRVFRNEGTSTRHNPEFTMLESYAAYEDYEDVMDLVEGLVSTVCKQVN
HHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
GTTEITYGEYKVDLTPNWRRVHMADVVKEYVGVDFWNVTSDEEARELAKKHDVAITEHMT
CCEEEEECEEEEEECCCCCEEHHHHHHHHHHCCCEECCCCCHHHHHHHHHCCCCHHHHHH
YGHILNEFFETYVEEKLIQPTFVYGHPVEISPLAKKNKEDERFTDRFELFIVGREHANAF
HHHHHHHHHHHHHHHHHCCCEEEECCCCEECCHHHCCCCHHHHHCEEEEEEEECHHHHHH
SELNDPIDQRERFEAQMKEREQGNDEAHGMDADFLEALEYGLPPTGGLGIGVDRLVMLLT
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
DAPSIRDILLFPTMKHRD
CCCCCCCEEECCCCCCCC
>Mature Secondary Structure 
SNENHEELNDQLIVRREKVDTLREEGIDPFGEKFIRSISPEEIETKFADKSKEELEEAA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHH
IEVSVAGRIMTKRVKGKVGFTHIQDRFHQLQIYIRKDAIGEDAYAIFKLADLGDIIGIKG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHCCCCCCCCE
TIFRTNTGELSVKATDFTLLSKSLRPLPDKYHGLKDVEQRYRQRYLDLITNEESQNRFVM
EEEECCCCCEEEEECHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
RSKILKYTRDYMDSQGFLEVETPVLHTIAGGAAAKPFITHHNALDMELYLRIALELHLKR
HHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHH
LIVGGMDKVYEIGRVFRNEGTSTRHNPEFTMLESYAAYEDYEDVMDLVEGLVSTVCKQVN
HHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
GTTEITYGEYKVDLTPNWRRVHMADVVKEYVGVDFWNVTSDEEARELAKKHDVAITEHMT
CCEEEEECEEEEEECCCCCEEHHHHHHHHHHCCCEECCCCCHHHHHHHHHCCCCHHHHHH
YGHILNEFFETYVEEKLIQPTFVYGHPVEISPLAKKNKEDERFTDRFELFIVGREHANAF
HHHHHHHHHHHHHHHHHCCCEEEECCCCEECCHHHCCCCHHHHHCEEEEEEEECHHHHHH
SELNDPIDQRERFEAQMKEREQGNDEAHGMDADFLEALEYGLPPTGGLGIGVDRLVMLLT
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
DAPSIRDILLFPTMKHRD
CCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]