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The map label for this gene is dnaA

Identifier: 116871423

GI number: 116871423

Start: 318

End: 1673

Strand: Direct

Name: dnaA

Synonym: lwe0001

Alternate gene names: 116871423

Gene position: 318-1673 (Clockwise)

Preceding gene: NA

Following gene: 116871424

Centisome position: 0.01

GC content: 36.65

Gene sequence:

>1356_bases
GTGCAATCAATTGAAGACATCTGGCAGGAAACTCTGCAAATCGTTAAAAAAAATATGAGTAAACCTAGTTACGATACATG
GATGAAATCAACAACCGCTCATTCATTAGAAGGTAATACGTTTATTATTTCAGCGCCCAATAATTTTGTTCGCGATTGGT
TAGAGAAGAGTTACACGCAATTTATCGCTAACATTTTGCAAGAAATAACTGGTCGCTTATTTGATGTCCGCTTTATTGAT
GGCGAGCAGGAAGAAAACTTTGAATATACGGTGATTAAACCAAATCCAGCGTTAGATGAAGATGGTATTGAAATTGGCAA
ACATATGCTTAATCCGCGTTATGTTTTCGATACATTTGTCATTGGTTCAGGGAACAGATTCGCCCACGCAGCATCACTTG
CAGTAGCCGAAGCTCCAGCAAAAGCATATAATCCGCTCTTCATTTACGGCGGAGTTGGGCTTGGTAAAACACATTTAATG
CATGCAGTTGGCCACTATGTTCAGCAACATAAAGATAATGCCAAAGTAATGTACCTTTCCAGCGAAAAATTCACTAATGA
GTTTATTAGCTCCATTCGAGATAATAAAACCGAAGAATTCCGTACAAAATACCGGAATGTCGATGTCTTACTTATTGATG
ATATCCAATTTTTAGCCGGTAAAGAAGGAACACAAGAGGAATTTTTCCATACATTTAATACACTTTATGATGAACAAAAA
CAAATTATTATTTCTAGTGACCGACCACCAAAAGAAATTCCTACACTGGAAGATCGGCTTAGATCCCGCTTTGAATGGGG
CTTAATTACTGATATTACGCCACCAGACTTAGAAACACGGATTGCTATTTTACGTAAAAAAGCAAAAGCAGACGGATTAG
ATATTCCAAATGAAGTCATGCTTTATATTGCAAACCAAATTGATTCGAATATTCGCGAGTTAGAAGGCGCACTCATTCGG
GTAGTTGCTTATTCTTCACTTGTTAATAAAGATATAACAGCTGGTCTTGCGGCAGAAGCACTAAAAGATATTATCCCCTC
TTCTAAATCACAAGTTATTACAATTAGTGGTATTCAAGAAACAGTGGGCGAATATTTCCACGTTCGTTTGGAAGATTTTA
AAGCAAAAAAACGGACGAAAAGTATTGCATTTCCTCGCCAAATCGCTATGTATCTTTCAAGAGAACTTACAGATGCTTCA
TTACCTAAAATCGGTGATGAATTTGGTGGACGAGATCATACCACCGTCATCCATGCACATGAAAAAATATCGCAACTACT
AAAAACCGATCAAGTGTTGAAAAATGACCTTGCTGAAATTGAAAAAAATTTAAGAAAATCACAAAATATGTTTTAA

Upstream 100 bases:

>100_bases
TAATTTTTTAACAGTGTTTGGATAACCTTATCCATGGCTTTTTCTATCTGTGGATAACTTATAGCATCCATTTACATTAC
TTAAAAAAGGGGGGTTACTA

Downstream 100 bases:

>100_bases
TAGACCTGTGTACAATGTGGATAACTGAAACATACTTACCCACAAGTTATCAACATGTGGAAAACTTTATGACGTATAGC
TTAGAACCTACTTATCCACA

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MQSIEDIWQETLQIVKKNMSKPSYDTWMKSTTAHSLEGNTFIISAPNNFVRDWLEKSYTQFIANILQEITGRLFDVRFID
GEQEENFEYTVIKPNPALDEDGIEIGKHMLNPRYVFDTFVIGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLM
HAVGHYVQQHKDNAKVMYLSSEKFTNEFISSIRDNKTEEFRTKYRNVDVLLIDDIQFLAGKEGTQEEFFHTFNTLYDEQK
QIIISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKADGLDIPNEVMLYIANQIDSNIRELEGALIR
VVAYSSLVNKDITAGLAAEALKDIIPSSKSQVITISGIQETVGEYFHVRLEDFKAKKRTKSIAFPRQIAMYLSRELTDAS
LPKIGDEFGGRDHTTVIHAHEKISQLLKTDQVLKNDLAEIEKNLRKSQNMF

Sequences:

>Translated_451_residues
MQSIEDIWQETLQIVKKNMSKPSYDTWMKSTTAHSLEGNTFIISAPNNFVRDWLEKSYTQFIANILQEITGRLFDVRFID
GEQEENFEYTVIKPNPALDEDGIEIGKHMLNPRYVFDTFVIGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLM
HAVGHYVQQHKDNAKVMYLSSEKFTNEFISSIRDNKTEEFRTKYRNVDVLLIDDIQFLAGKEGTQEEFFHTFNTLYDEQK
QIIISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKADGLDIPNEVMLYIANQIDSNIRELEGALIR
VVAYSSLVNKDITAGLAAEALKDIIPSSKSQVITISGIQETVGEYFHVRLEDFKAKKRTKSIAFPRQIAMYLSRELTDAS
LPKIGDEFGGRDHTTVIHAHEKISQLLKTDQVLKNDLAEIEKNLRKSQNMF
>Mature_451_residues
MQSIEDIWQETLQIVKKNMSKPSYDTWMKSTTAHSLEGNTFIISAPNNFVRDWLEKSYTQFIANILQEITGRLFDVRFID
GEQEENFEYTVIKPNPALDEDGIEIGKHMLNPRYVFDTFVIGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLM
HAVGHYVQQHKDNAKVMYLSSEKFTNEFISSIRDNKTEEFRTKYRNVDVLLIDDIQFLAGKEGTQEEFFHTFNTLYDEQK
QIIISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKADGLDIPNEVMLYIANQIDSNIRELEGALIR
VVAYSSLVNKDITAGLAAEALKDIIPSSKSQVITISGIQETVGEYFHVRLEDFKAKKRTKSIAFPRQIAMYLSRELTDAS
LPKIGDEFGGRDHTTVIHAHEKISQLLKTDQVLKNDLAEIEKNLRKSQNMF

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=463, Percent_Identity=42.9805615550756, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI226510964, Length=198, Percent_Identity=25.2525252525253, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA_LISIN (Q92FV2)

Other databases:

- EMBL:   AL596163
- PIR:   AB1433
- RefSeq:   NP_469348.1
- ProteinModelPortal:   Q92FV2
- GeneID:   1128350
- GenomeReviews:   AL592022_GR
- KEGG:   lin:lin0001
- GenoList:   LIN0001
- HOGENOM:   HBG695666
- OMA:   IQRVVGQ
- ProtClustDB:   PRK00149
- BioCyc:   LINN272626:LIN0001-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 51428; Mature: 51428

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSIEDIWQETLQIVKKNMSKPSYDTWMKSTTAHSLEGNTFIISAPNNFVRDWLEKSYTQ
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHH
FIANILQEITGRLFDVRFIDGEQEENFEYTVIKPNPALDEDGIEIGKHMLNPRYVFDTFV
HHHHHHHHHHHHEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEE
IGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLMHAVGHYVQQHKDNAKVMYLS
ECCCCCHHHHHHHHHHHCCHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
SEKFTNEFISSIRDNKTEEFRTKYRNVDVLLIDDIQFLAGKEGTQEEFFHTFNTLYDEQK
CHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCHHHHHCCCCCHHHHHHHHHHHHHCCC
QIIISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKADGLDIPNEVM
EEEEECCCCCCCCCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHH
LYIANQIDSNIRELEGALIRVVAYSSLVNKDITAGLAAEALKDIIPSSKSQVITISGIQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHH
TVGEYFHVRLEDFKAKKRTKSIAFPRQIAMYLSRELTDASLPKIGDEFGGRDHTTVIHAH
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCEEEEHH
EKISQLLKTDQVLKNDLAEIEKNLRKSQNMF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQSIEDIWQETLQIVKKNMSKPSYDTWMKSTTAHSLEGNTFIISAPNNFVRDWLEKSYTQ
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHH
FIANILQEITGRLFDVRFIDGEQEENFEYTVIKPNPALDEDGIEIGKHMLNPRYVFDTFV
HHHHHHHHHHHHEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEE
IGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLMHAVGHYVQQHKDNAKVMYLS
ECCCCCHHHHHHHHHHHCCHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
SEKFTNEFISSIRDNKTEEFRTKYRNVDVLLIDDIQFLAGKEGTQEEFFHTFNTLYDEQK
CHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCHHHHHCCCCCHHHHHHHHHHHHHCCC
QIIISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKADGLDIPNEVM
EEEEECCCCCCCCCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHH
LYIANQIDSNIRELEGALIRVVAYSSLVNKDITAGLAAEALKDIIPSSKSQVITISGIQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHH
TVGEYFHVRLEDFKAKKRTKSIAFPRQIAMYLSRELTDASLPKIGDEFGGRDHTTVIHAH
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCEEEEHH
EKISQLLKTDQVLKNDLAEIEKNLRKSQNMF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669