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The map label for this gene is dnaN [H]

Identifier: 116871424

GI number: 116871424

Start: 1867

End: 3012

Strand: Direct

Name: dnaN [H]

Synonym: lwe0002

Alternate gene names: 116871424

Gene position: 1867-3012 (Clockwise)

Preceding gene: 116871423

Following gene: 116871425

Centisome position: 0.07

GC content: 33.86

Gene sequence:

>1146_bases
ATGAAATTTGTTATTGAGCGTGATCGTCTTGTCCAAGCAGTCAATGAAGTTACTCGTGCCATCTCTGCAAGAACAACGAT
TCCAATTTTAACGGGAATAAAAATAGTCGTAAATGATGAAGGTGTAACACTAACTGGTAGTGATTCAGATATTTCCATCG
AAGCATTTATACCATTAATTGAAAATGATGAAGTAATTGTAGAAGTGGAAAGTTTTGGAGGTATTGTTCTTCAATCCAAG
TACTTCGGCGATATCGTTCGTCGTTTACCTGAAGAAAATGTAGAAATTGAAGTAACAACAAATTATCAAACTAATATTAG
TTCTGGCCAAGCATCCTTTACGCTAAATGGTTTAGATCCAATGGAATATCCTAAATTACCAGAAGTTACAGATGGTAAAA
ATATTAAAATTCCAATTAATGTCCTTAAAAATATCATTAGACAAACTGTTTTTGCTGTTTCTGCAATTGAAGTTCGCCCA
GTTCTTACTGGTGTTAACTGGATTATTAAAGAAAATAAACTGAGTGCAGTTGCAACAGATAGTCATCGTCTAGCTTTACG
TGAAATTCCACTGGAAACAGACATTGATGAAGAATATAACATTGTTATTCCAGGAAAAAGCTTAGCTGAACTAAACAAAA
TTTTAGATGATGCAAGTGAATCTATTGAAATGACACTCGCAAATAACCAAATACTATTTAAATTAAAAGATTTATTATTC
TATTCTCGTTTACTAGAAGGTAGTTATCCTGATACGTCGCGTTTAATTCCAACCGACACTAAATCAGAACTAGTTATTAA
TTCAAGAGCATTTTTACAAGCGATTGATCGTGCTTCCCTACTTGCTCGTGAAAATCGTAATAACGTTATCAAATTAATGA
CACTTGAAAATGGACAAGTTGAAGTATCCTCTAACTCTCCTGAAGTTGGGAATGTTTCAGAAAACGTCTTTAGTCAAAGT
TTTACTGGTGAAGAAATTAAAATCTCCTTCAACGGTAAATACATGATGGATGCATTGCGTGCATTTGAAGGTGATGATAT
TCAAATCTCCTTCTCAGGTACAATGAGACCATTCGTGCTTCGACCAAAAGATGCTACGAATCCAAATGAAATTTTACAAT
TAATCACGCCGGTTAGAACTTACTAA

Upstream 100 bases:

>100_bases
ATCCACAAATCCACAGCGCCTATTACTATTACTACGATTTTTTATTAATTAATTAAAGGTTTTTGAGGTTTTACAAAATA
TAAAAAAATGGGGGATACTC

Downstream 100 bases:

>100_bases
TCTTGCAAAAAAGCACATCCGTCTTGGTTTCAAGGCGGATTCTTTTTTTGTTATAGTACAGTTTTTTAGAAAAAATGTAT
ATTTATGCAAACGGGGGTAA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKFVIERDRLVQAVNEVTRAISARTTIPILTGIKIVVNDEGVTLTGSDSDISIEAFIPLIENDEVIVEVESFGGIVLQSK
YFGDIVRRLPEENVEIEVTTNYQTNISSGQASFTLNGLDPMEYPKLPEVTDGKNIKIPINVLKNIIRQTVFAVSAIEVRP
VLTGVNWIIKENKLSAVATDSHRLALREIPLETDIDEEYNIVIPGKSLAELNKILDDASESIEMTLANNQILFKLKDLLF
YSRLLEGSYPDTSRLIPTDTKSELVINSRAFLQAIDRASLLARENRNNVIKLMTLENGQVEVSSNSPEVGNVSENVFSQS
FTGEEIKISFNGKYMMDALRAFEGDDIQISFSGTMRPFVLRPKDATNPNEILQLITPVRTY

Sequences:

>Translated_381_residues
MKFVIERDRLVQAVNEVTRAISARTTIPILTGIKIVVNDEGVTLTGSDSDISIEAFIPLIENDEVIVEVESFGGIVLQSK
YFGDIVRRLPEENVEIEVTTNYQTNISSGQASFTLNGLDPMEYPKLPEVTDGKNIKIPINVLKNIIRQTVFAVSAIEVRP
VLTGVNWIIKENKLSAVATDSHRLALREIPLETDIDEEYNIVIPGKSLAELNKILDDASESIEMTLANNQILFKLKDLLF
YSRLLEGSYPDTSRLIPTDTKSELVINSRAFLQAIDRASLLARENRNNVIKLMTLENGQVEVSSNSPEVGNVSENVFSQS
FTGEEIKISFNGKYMMDALRAFEGDDIQISFSGTMRPFVLRPKDATNPNEILQLITPVRTY
>Mature_381_residues
MKFVIERDRLVQAVNEVTRAISARTTIPILTGIKIVVNDEGVTLTGSDSDISIEAFIPLIENDEVIVEVESFGGIVLQSK
YFGDIVRRLPEENVEIEVTTNYQTNISSGQASFTLNGLDPMEYPKLPEVTDGKNIKIPINVLKNIIRQTVFAVSAIEVRP
VLTGVNWIIKENKLSAVATDSHRLALREIPLETDIDEEYNIVIPGKSLAELNKILDDASESIEMTLANNQILFKLKDLLF
YSRLLEGSYPDTSRLIPTDTKSELVINSRAFLQAIDRASLLARENRNNVIKLMTLENGQVEVSSNSPEVGNVSENVFSQS
FTGEEIKISFNGKYMMDALRAFEGDDIQISFSGTMRPFVLRPKDATNPNEILQLITPVRTY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=353, Percent_Identity=28.328611898017, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42513; Mature: 42513

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVIERDRLVQAVNEVTRAISARTTIPILTGIKIVVNDEGVTLTGSDSDISIEAFIPLI
CCEEECHHHHHHHHHHHHHHHHCCCCEEEEECEEEEECCCCEEEECCCCCEEEEEEEEEE
ENDEVIVEVESFGGIVLQSKYFGDIVRRLPEENVEIEVTTNYQTNISSGQASFTLNGLDP
CCCEEEEEEECCCCEEEEHHHHHHHHHHCCCCCEEEEEEECEEECCCCCCEEEEECCCCC
MEYPKLPEVTDGKNIKIPINVLKNIIRQTVFAVSAIEVRPVLTGVNWIIKENKLSAVATD
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHEEEEEEEECCEEEEECCCEEEEECC
SHRLALREIPLETDIDEEYNIVIPGKSLAELNKILDDASESIEMTLANNQILFKLKDLLF
CCEEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCEEEEEECCEEEEHHHHHHH
YSRLLEGSYPDTSRLIPTDTKSELVINSRAFLQAIDRASLLARENRNNVIKLMTLENGQV
HHHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEE
EVSSNSPEVGNVSENVFSQSFTGEEIKISFNGKYMMDALRAFEGDDIQISFSGTMRPFVL
EECCCCCCCCCCHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCEEEEECCCCCEEEE
RPKDATNPNEILQLITPVRTY
CCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFVIERDRLVQAVNEVTRAISARTTIPILTGIKIVVNDEGVTLTGSDSDISIEAFIPLI
CCEEECHHHHHHHHHHHHHHHHCCCCEEEEECEEEEECCCCEEEECCCCCEEEEEEEEEE
ENDEVIVEVESFGGIVLQSKYFGDIVRRLPEENVEIEVTTNYQTNISSGQASFTLNGLDP
CCCEEEEEEECCCCEEEEHHHHHHHHHHCCCCCEEEEEEECEEECCCCCCEEEEECCCCC
MEYPKLPEVTDGKNIKIPINVLKNIIRQTVFAVSAIEVRPVLTGVNWIIKENKLSAVATD
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHEEEEEEEECCEEEEECCCEEEEECC
SHRLALREIPLETDIDEEYNIVIPGKSLAELNKILDDASESIEMTLANNQILFKLKDLLF
CCEEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCEEEEEECCEEEEHHHHHHH
YSRLLEGSYPDTSRLIPTDTKSELVINSRAFLQAIDRASLLARENRNNVIKLMTLENGQV
HHHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEE
EVSSNSPEVGNVSENVFSQSFTGEEIKISFNGKYMMDALRAFEGDDIQISFSGTMRPFVL
EECCCCCCCCCCHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCEEEEECCCCCEEEE
RPKDATNPNEILQLITPVRTY
CCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]