Click here to switch to the map view.

The map label for this gene is gshA [H]

Identifier: 116691167

GI number: 116691167

Start: 3469017

End: 3470618

Strand: Reverse

Name: gshA [H]

Synonym: Bcen2424_3149

Alternate gene names: 116691167

Gene position: 3470618-3469017 (Counterclockwise)

Preceding gene: 116691168

Following gene: 116691165

Centisome position: 99.62

GC content: 67.04

Gene sequence:

>1602_bases
ATGACTCACCGCCAGTCCGAACTGCTGCTGAAACGCCTCGACGCGCTGAGCTCGGGCCCGACGCGGCAGCATCTGCCCGA
CGGCCTGCGCGGCATCGAGAAGGAAAGCCTGCGCGTGACGCGCGACGGGATGATCGCGTTCACGCCGCATCCGCGCGCGC
TCGGTTCGGCGCTCACGCATCCGGCGCTGACGACCGACTATTCCGAAGCGCTGATCGAACTGATCACGCCCGCGGAGCGC
GACGCCGCGCTTACGCTCGAACGCCTCGACGATCTGCATCGCTACGTCTATGCATCGCTCGGCGACGAGATCCTGTGGAA
CGATTCGATGCCCGGCCTGCTGCCGGCCGACGACCAGATCCCGATCGCCAACTACGGCACGTCGAACATCGGCCGCCTGA
AGACGGTGTACCGGCGCGGCCTCGCGTATCGCTACGGCCGCACGATGCAGTGCATCGCCGGCATCCACTACAACTACTCG
TTGCACGAGGAAGTGTGGCGGCGCCTGCATGAGGAAGAAGAGTCGACGGCGACGCTCGTCGATTACCAGTCGGAACGCTA
TCTCGCGCAGATCCGCAACTTCCGCCGCCGCAGCTGGCTGCTGATGTACCTGTTCGGCGCGTCGCCGGTGCTCGACACGA
AGTTCCTGCGCGGCAAGCCGCACAAGCTCGATACGTTCGACGCCGATACGCTGTACCGGCCGTATGCGACCAGCCTGCGG
ATGAGCGATCTCGGCTACTCGAACACGACGGCCCAGGCCGCGCTGCACGTCGACTACAACACGCTGCCGGGCTATCTCGA
CGCGCTGTCGAAGGCCGTGAGCGAGCCGTATCCGGCGTACGAGGCGATCGGCACGCATCGCGACGGCGAGTGGATCCAGA
TCAACACGAACGTGCTGCAGATCGAGAACGAGTTCTATTCGACGATCCGGCCGAAGCGTGTCACGTACTCGGGCGAGCGG
CCGCTGCATGCGCTCGCGTCGCGCGGCGTGCAGTACATCGAAGTGCGCTGCCTCGACATCGACCCGTTCGAGCCGACCGG
CATCGCGCTGGAGACCGCGCGCTTCATCGACGCGTTCCTGCTCGCGTGCGCGCTCGACGACAGCGCGCCGCTCGACTGCG
ACGCGTACAAGGAAGCGAACGCGAACTTCGGCAGCGTGACGCTGGACGGCCGCAAGCCGGGGCTCGCGCTGACGCGCGAA
GGTCGATCGGTCACGCTGCAGCAGTGGGCCGATGAACTGATGGCCGATATTGAAATCGTCGGCCGCCGGCTCGACGAAAT
CCGCGGCGGCGACGAGCATGCGCGCGCGATCGCCGCGCAACGCGAGAAGCTCGCCGATCCGGAGCGCACGCCGTCGGCGC
GCGTGCTGCGCACGATGCGCGAGAACGGCCAGTCGTTCCTCGCGTTCGGGCTCGCGCAGAGCGAAGCGCATGCCGCGCAT
TTCCGCGCGCACCCGCCGTCGGCCGAGACGCTGCACACCGAGCAGGCGCTTGCCGCGAAGTCGCTGGCCGAGCAGGCCGA
GCTCGAAGCGAAGGAGGCCGGATCGTTCGACGCATTCGTCGCCGCCTATCGCGCCTATACGCTGAACCGCTTCAGCGTGT
GA

Upstream 100 bases:

>100_bases
TTAAAGTGCCCGCTACCAACGAAGCGACCAACAATCCAGCCGGCCGCGCGCGGCGTTCTGCGCGCGGCCGTCACGCACTT
GCGTCTCCATGTCGAACACC

Downstream 100 bases:

>100_bases
CGATCGCGCACGCGGCCGGCGCGGCGTGTGCCGCGCCGCTGCCGTGCGATCCGGCTGGTTCCATCGGTGAGCCGGTCAGT
GATGCGCATAAGTGCCGCGG

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MTHRQSELLLKRLDALSSGPTRQHLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPALTTDYSEALIELITPAER
DAALTLERLDDLHRYVYASLGDEILWNDSMPGLLPADDQIPIANYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHYNYS
LHEEVWRRLHEEEESTATLVDYQSERYLAQIRNFRRRSWLLMYLFGASPVLDTKFLRGKPHKLDTFDADTLYRPYATSLR
MSDLGYSNTTAQAALHVDYNTLPGYLDALSKAVSEPYPAYEAIGTHRDGEWIQINTNVLQIENEFYSTIRPKRVTYSGER
PLHALASRGVQYIEVRCLDIDPFEPTGIALETARFIDAFLLACALDDSAPLDCDAYKEANANFGSVTLDGRKPGLALTRE
GRSVTLQQWADELMADIEIVGRRLDEIRGGDEHARAIAAQREKLADPERTPSARVLRTMRENGQSFLAFGLAQSEAHAAH
FRAHPPSAETLHTEQALAAKSLAEQAELEAKEAGSFDAFVAAYRAYTLNRFSV

Sequences:

>Translated_533_residues
MTHRQSELLLKRLDALSSGPTRQHLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPALTTDYSEALIELITPAER
DAALTLERLDDLHRYVYASLGDEILWNDSMPGLLPADDQIPIANYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHYNYS
LHEEVWRRLHEEEESTATLVDYQSERYLAQIRNFRRRSWLLMYLFGASPVLDTKFLRGKPHKLDTFDADTLYRPYATSLR
MSDLGYSNTTAQAALHVDYNTLPGYLDALSKAVSEPYPAYEAIGTHRDGEWIQINTNVLQIENEFYSTIRPKRVTYSGER
PLHALASRGVQYIEVRCLDIDPFEPTGIALETARFIDAFLLACALDDSAPLDCDAYKEANANFGSVTLDGRKPGLALTRE
GRSVTLQQWADELMADIEIVGRRLDEIRGGDEHARAIAAQREKLADPERTPSARVLRTMRENGQSFLAFGLAQSEAHAAH
FRAHPPSAETLHTEQALAAKSLAEQAELEAKEAGSFDAFVAAYRAYTLNRFSV
>Mature_532_residues
THRQSELLLKRLDALSSGPTRQHLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPALTTDYSEALIELITPAERD
AALTLERLDDLHRYVYASLGDEILWNDSMPGLLPADDQIPIANYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHYNYSL
HEEVWRRLHEEEESTATLVDYQSERYLAQIRNFRRRSWLLMYLFGASPVLDTKFLRGKPHKLDTFDADTLYRPYATSLRM
SDLGYSNTTAQAALHVDYNTLPGYLDALSKAVSEPYPAYEAIGTHRDGEWIQINTNVLQIENEFYSTIRPKRVTYSGERP
LHALASRGVQYIEVRCLDIDPFEPTGIALETARFIDAFLLACALDDSAPLDCDAYKEANANFGSVTLDGRKPGLALTREG
RSVTLQQWADELMADIEIVGRRLDEIRGGDEHARAIAAQREKLADPERTPSARVLRTMRENGQSFLAFGLAQSEAHAAHF
RAHPPSAETLHTEQALAAKSLAEQAELEAKEAGSFDAFVAAYRAYTLNRFSV

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=504, Percent_Identity=42.8571428571429, Blast_Score=377, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 59851; Mature: 59720

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHRQSELLLKRLDALSSGPTRQHLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTH
CCCCHHHHHHHHHHHHCCCCCHHHCCHHHHCCCHHHEEEEECCEEEECCCCHHHHHHHCC
PALTTDYSEALIELITPAERDAALTLERLDDLHRYVYASLGDEILWNDSMPGLLPADDQI
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCC
PIANYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHYNYSLHEEVWRRLHEEEESTATLV
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEE
DYQSERYLAQIRNFRRRSWLLMYLFGASPVLDTKFLRGKPHKLDTFDADTLYRPYATSLR
ECHHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHEE
MSDLGYSNTTAQAALHVDYNTLPGYLDALSKAVSEPYPAYEAIGTHRDGEWIQINTNVLQ
HHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEECCEEE
IENEFYSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEPTGIALETARFIDAFL
ECHHHHHHCCCCEEEECCCCCHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHH
LACALDDSAPLDCDAYKEANANFGSVTLDGRKPGLALTREGRSVTLQQWADELMADIEIV
HHHHCCCCCCCCCCHHHHCCCCCCEEEECCCCCCEEEECCCCEEEHHHHHHHHHHHHHHH
GRRLDEIRGGDEHARAIAAQREKLADPERTPSARVLRTMRENGQSFLAFGLAQSEAHAAH
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEHHHCCHHHHH
FRAHPPSAETLHTEQALAAKSLAEQAELEAKEAGSFDAFVAAYRAYTLNRFSV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
THRQSELLLKRLDALSSGPTRQHLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTH
CCCHHHHHHHHHHHHCCCCCHHHCCHHHHCCCHHHEEEEECCEEEECCCCHHHHHHHCC
PALTTDYSEALIELITPAERDAALTLERLDDLHRYVYASLGDEILWNDSMPGLLPADDQI
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCC
PIANYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHYNYSLHEEVWRRLHEEEESTATLV
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEE
DYQSERYLAQIRNFRRRSWLLMYLFGASPVLDTKFLRGKPHKLDTFDADTLYRPYATSLR
ECHHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHEE
MSDLGYSNTTAQAALHVDYNTLPGYLDALSKAVSEPYPAYEAIGTHRDGEWIQINTNVLQ
HHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEECCEEE
IENEFYSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEPTGIALETARFIDAFL
ECHHHHHHCCCCEEEECCCCCHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHH
LACALDDSAPLDCDAYKEANANFGSVTLDGRKPGLALTREGRSVTLQQWADELMADIEIV
HHHHCCCCCCCCCCHHHHCCCCCCEEEECCCCCCEEEECCCCEEEHHHHHHHHHHHHHHH
GRRLDEIRGGDEHARAIAAQREKLADPERTPSARVLRTMRENGQSFLAFGLAQSEAHAAH
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEHHHCCHHHHH
FRAHPPSAETLHTEQALAAKSLAEQAELEAKEAGSFDAFVAAYRAYTLNRFSV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA