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The map label for this gene is phnA [H]

Identifier: 116691160

GI number: 116691160

Start: 3458949

End: 3460187

Strand: Reverse

Name: phnA [H]

Synonym: Bcen2424_3142

Alternate gene names: 116691160

Gene position: 3460187-3458949 (Counterclockwise)

Preceding gene: 116691162

Following gene: 116691159

Centisome position: 99.32

GC content: 67.39

Gene sequence:

>1239_bases
ATGACTGAAACGCCTGTATCCGTGGAAGTGAACGGCCGCCGCTACAACTGGATGAGCCGGCCCGTGGTGGTCGTCTGCGT
CGATGGCTGCGCATACGAATATCTGGAGGAAGCCGCCGAGGCCGGCGTCGCGCCGTTCCTGCGCACGCTGCTGAAGCCCG
GCACGGCGCTCAAGGGCGAGTGCGTGGTGCCGAGTTTCACCAATCCGAACAACCTGTCGATCGTCACCGGCGTGCCGCCG
GCGATCCACGGGATAAGCGGCAACTACTTCTACGATCGCGACACCGGCGCCGAGGTGCTGATGAACGATCCGAAGTACCT
CGTCGCGCCCACCGTGCTCGCGACCTTCGCCGAGCAGGGTGCGCGCGTCGCGGTCGTCACCGCGAAGGACAAGCTGCGCC
GCCTGCTCGGCAAGGGGCTCAAGGGCATCTGCTTCTCGTCGGAAAAGGCCGATGAAGCGAGCGTCGAGGAAAACGGCATC
GACCACGTGCTCGAGCTGGTCGGCAAGCCGGTGCCGAGCGTGTACAGCGCGGACCTGTCGGAATTCGTGTTCGCGGCCGG
CGTGCGCCTGCTCGAGACGCGCCCGATCGACCTGATGTACCTGTCGACGACCGACTACGTGCAGCACAAGTGCGCACCGG
GCACGCCCGGCGCGAACGCGTTCTACGCGATGATGGACACCTACCTGCAGCGCCTCGACGCACTCGGCGCGATCGTCGCG
ATCACCGCCGATCACGGGATGAACGCGAAGCACGACGACGCGACCGGCGAGCCGAACGTGATCTACCTGCAGGAGCTGTT
CGACGAATGGCTCGGCCACGATGCGGCGCGTGTGATCCTGCCGATCACCGACCCGTACGTCGTGCACCACGGCGCGCTCG
GTTCGTTCGCGACCGTCTACGTGCCTGGCGACGCCGACACCGACGCGCTGCGCGCGCGGCTCGCCGCGGTCGACGGCATC
GAGGTCGTGCTGACGGGCGCCGAAGGCTGCGCGCGCTTCGAGCTGCCGCCCGCGCGGATGGGCGACCTGATCGTGATCTC
GAAGCAGGACGTCGTGCTCGGCACGCGCCGCATCAAGCACGACCTGTCGGGCCTCGACGTGCCGCTGCGCTCGCACGGCG
GGATCTCCGAGCAGATCGTGCCGCTGATCTTCAGCAAGCCGGTCGCGACCGACGTCACGGGCCGCGCGCGGCTGCGCAAC
TTCGACATCATCGACATCGCGCTCAACCATCTGCAGTGA

Upstream 100 bases:

>100_bases
CTACAAATCATCGACTTTAATGTCAAGCGTCGAAAACAGCAGTGGCCACGGCGCGGGCGCCGACCGCTCAACCCCCTTCG
AACCGAAAAGAATGAACGTC

Downstream 100 bases:

>100_bases
CGCGGCCGCATCCGCCAGGAGACACTCATGAACGCCATTGCAGCATCGACGGATGTCCGCACGCCTCATCGTGAAGCACT
GCGAATCGACGGTGAAAAGA

Product: phosphonoacetate hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MTETPVSVEVNGRRYNWMSRPVVVVCVDGCAYEYLEEAAEAGVAPFLRTLLKPGTALKGECVVPSFTNPNNLSIVTGVPP
AIHGISGNYFYDRDTGAEVLMNDPKYLVAPTVLATFAEQGARVAVVTAKDKLRRLLGKGLKGICFSSEKADEASVEENGI
DHVLELVGKPVPSVYSADLSEFVFAAGVRLLETRPIDLMYLSTTDYVQHKCAPGTPGANAFYAMMDTYLQRLDALGAIVA
ITADHGMNAKHDDATGEPNVIYLQELFDEWLGHDAARVILPITDPYVVHHGALGSFATVYVPGDADTDALRARLAAVDGI
EVVLTGAEGCARFELPPARMGDLIVISKQDVVLGTRRIKHDLSGLDVPLRSHGGISEQIVPLIFSKPVATDVTGRARLRN
FDIIDIALNHLQ

Sequences:

>Translated_412_residues
MTETPVSVEVNGRRYNWMSRPVVVVCVDGCAYEYLEEAAEAGVAPFLRTLLKPGTALKGECVVPSFTNPNNLSIVTGVPP
AIHGISGNYFYDRDTGAEVLMNDPKYLVAPTVLATFAEQGARVAVVTAKDKLRRLLGKGLKGICFSSEKADEASVEENGI
DHVLELVGKPVPSVYSADLSEFVFAAGVRLLETRPIDLMYLSTTDYVQHKCAPGTPGANAFYAMMDTYLQRLDALGAIVA
ITADHGMNAKHDDATGEPNVIYLQELFDEWLGHDAARVILPITDPYVVHHGALGSFATVYVPGDADTDALRARLAAVDGI
EVVLTGAEGCARFELPPARMGDLIVISKQDVVLGTRRIKHDLSGLDVPLRSHGGISEQIVPLIFSKPVATDVTGRARLRN
FDIIDIALNHLQ
>Mature_411_residues
TETPVSVEVNGRRYNWMSRPVVVVCVDGCAYEYLEEAAEAGVAPFLRTLLKPGTALKGECVVPSFTNPNNLSIVTGVPPA
IHGISGNYFYDRDTGAEVLMNDPKYLVAPTVLATFAEQGARVAVVTAKDKLRRLLGKGLKGICFSSEKADEASVEENGID
HVLELVGKPVPSVYSADLSEFVFAAGVRLLETRPIDLMYLSTTDYVQHKCAPGTPGANAFYAMMDTYLQRLDALGAIVAI
TADHGMNAKHDDATGEPNVIYLQELFDEWLGHDAARVILPITDPYVVHHGALGSFATVYVPGDADTDALRARLAAVDGIE
VVLTGAEGCARFELPPARMGDLIVISKQDVVLGTRRIKHDLSGLDVPLRSHGGISEQIVPLIFSKPVATDVTGRARLRNF
DIIDIALNHLQ

Specific function: Specifically hydrolyzes phosphonoacetate. Does not have activity on other organophosphonates or acetates [H]

COG id: COG1524

COG function: function code R; Uncharacterized proteins of the AP superfamily

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family. PhnA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR002591
- InterPro:   IPR012710
- InterPro:   IPR023116 [H]

Pfam domain/function: PF01663 Phosphodiest [H]

EC number: =3.11.1.2 [H]

Molecular weight: Translated: 44453; Mature: 44322

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETPVSVEVNGRRYNWMSRPVVVVCVDGCAYEYLEEAAEAGVAPFLRTLLKPGTALKGE
CCCCCEEEEECCEEEECCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
CVVPSFTNPNNLSIVTGVPPAIHGISGNYFYDRDTGAEVLMNDPKYLVAPTVLATFAEQG
EECCCCCCCCCEEEEECCCHHHCCCCCCEEEECCCCCEEEECCCCEEEHHHHHHHHHHCC
ARVAVVTAKDKLRRLLGKGLKGICFSSEKADEASVEENGIDHVLELVGKPVPSVYSADLS
CEEEEEECHHHHHHHHHCCCCEEEECCCCCCCCCHHHCCHHHHHHHHCCCCCCHHHHHHH
EFVFAAGVRLLETRPIDLMYLSTTDYVQHKCAPGTPGANAFYAMMDTYLQRLDALGAIVA
HHHHHHCCCEEECCCEEEEEEECCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEE
ITADHGMNAKHDDATGEPNVIYLQELFDEWLGHDAARVILPITDPYVVHHGALGSFATVY
EEECCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCEEEEE
VPGDADTDALRARLAAVDGIEVVLTGAEGCARFELPPARMGDLIVISKQDVVLGTRRIKH
ECCCCCHHHHHHHHHHCCCEEEEEECCCCCEECCCCCCCCCCEEEEECCCEEEHHHHHHH
DLSGLDVPLRSHGGISEQIVPLIFSKPVATDVTGRARLRNFDIIDIALNHLQ
HHCCCCCCHHCCCCCCHHHHHHHCCCCCCCCCCCCHHHCCCCEEEHHHHHCC
>Mature Secondary Structure 
TETPVSVEVNGRRYNWMSRPVVVVCVDGCAYEYLEEAAEAGVAPFLRTLLKPGTALKGE
CCCCEEEEECCEEEECCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
CVVPSFTNPNNLSIVTGVPPAIHGISGNYFYDRDTGAEVLMNDPKYLVAPTVLATFAEQG
EECCCCCCCCCEEEEECCCHHHCCCCCCEEEECCCCCEEEECCCCEEEHHHHHHHHHHCC
ARVAVVTAKDKLRRLLGKGLKGICFSSEKADEASVEENGIDHVLELVGKPVPSVYSADLS
CEEEEEECHHHHHHHHHCCCCEEEECCCCCCCCCHHHCCHHHHHHHHCCCCCCHHHHHHH
EFVFAAGVRLLETRPIDLMYLSTTDYVQHKCAPGTPGANAFYAMMDTYLQRLDALGAIVA
HHHHHHCCCEEECCCEEEEEEECCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEE
ITADHGMNAKHDDATGEPNVIYLQELFDEWLGHDAARVILPITDPYVVHHGALGSFATVY
EEECCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCEEEEE
VPGDADTDALRARLAAVDGIEVVLTGAEGCARFELPPARMGDLIVISKQDVVLGTRRIKH
ECCCCCHHHHHHHHHHCCCEEEEEECCCCCEECCCCCCCCCCEEEEECCCEEEHHHHHHH
DLSGLDVPLRSHGGISEQIVPLIFSKPVATDVTGRARLRNFDIIDIALNHLQ
HHCCCCCCHHCCCCCCHHHHHHHCCCCCCCCCCCCHHHCCCCEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA