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The map label for this gene is tcyA [H]

Identifier: 116691158

GI number: 116691158

Start: 3456504

End: 3457286

Strand: Reverse

Name: tcyA [H]

Synonym: Bcen2424_3140

Alternate gene names: 116691158

Gene position: 3457286-3456504 (Counterclockwise)

Preceding gene: 116691159

Following gene: 116691157

Centisome position: 99.24

GC content: 65.64

Gene sequence:

>783_bases
ATGAAATTCCTCCGCTCCATTCTGGTCGTCGCGCTGCTGCAGGCTGCCGCGGCCACCAGTGCGCTGGCTGCCGACGACCT
GTCGCAGATCAAGTCGGCCGGCGTGTTCCGCGTCGGCACCGAAGGCACCTATGCGCCGTTCACGTACCACGACGAGACCG
GCAAGCTGACGGGCTTCGACGTCGACATCGCGACCGCGATCGCGCAGCGCCTCGGGGTGAAGCCGCAGTTCGTCGAAGGC
AAATGGGACGGGCTGATCGCGGGGCTCGACGTGAACCGCTACGACGCGGTCGTCAACGAAGTGTCGATCACCGACGCGCG
CAAGGCGAAATACGATTTCTCGACGCCGTACATCACGTCGCACGCGGTGCTGATCGTGCGCGCGGACAACACGACGATCC
GCTCGTTCGACGACCTGAAGGGCAAGAAATCCGCGAACACGCTGACCAGCAATTTCGGCAAGCTCGCGGCCGCGCACGGC
GCCGACGTGGTGCCCGTGCAGGGCTTCAACGAATCGATCGACCTGCTGACGTCCGGCCGCGTCGATGCGACGATCAACGA
CTCGCTGTCGTACCTCGATTTCCGCAAGCACAAGCCCGACGCGAAACTGAAGATCGCGGCGACCGACACGACCGGCGCGG
GCGACGCATCCGGCGTGCTGCTGCGCAAGGGCAGCCCCGCGCTGGTGGCGGCGATCGACAAGGCGCTCGCGGACATCAAG
GCGGACGGTACCTACGCGAAGATCTCGCAGAAGTACTTCGGCCGCGACGTGTCGCAGCCGTGA

Upstream 100 bases:

>100_bases
TTTTTGCCGGCCCTCCACGCATCGGTAGAATTCGCGGACCTCTCGAACGGCGCAGGCGCGCCGGCGGGATGATCCTCTCC
AATCATTCGGGGTCCGCACC

Downstream 100 bases:

>100_bases
CGCGAGGCTGAACGATGCCGGCCTGGCTGCACCTGATGGCGGAATCGCTGCGGCCCCTGCTCGTTGCAGGGCTCGTGTTT
ACGGTTCCGCTCACGCTCGC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKFLRSILVVALLQAAAATSALAADDLSQIKSAGVFRVGTEGTYAPFTYHDETGKLTGFDVDIATAIAQRLGVKPQFVEG
KWDGLIAGLDVNRYDAVVNEVSITDARKAKYDFSTPYITSHAVLIVRADNTTIRSFDDLKGKKSANTLTSNFGKLAAAHG
ADVVPVQGFNESIDLLTSGRVDATINDSLSYLDFRKHKPDAKLKIAATDTTGAGDASGVLLRKGSPALVAAIDKALADIK
ADGTYAKISQKYFGRDVSQP

Sequences:

>Translated_260_residues
MKFLRSILVVALLQAAAATSALAADDLSQIKSAGVFRVGTEGTYAPFTYHDETGKLTGFDVDIATAIAQRLGVKPQFVEG
KWDGLIAGLDVNRYDAVVNEVSITDARKAKYDFSTPYITSHAVLIVRADNTTIRSFDDLKGKKSANTLTSNFGKLAAAHG
ADVVPVQGFNESIDLLTSGRVDATINDSLSYLDFRKHKPDAKLKIAATDTTGAGDASGVLLRKGSPALVAAIDKALADIK
ADGTYAKISQKYFGRDVSQP
>Mature_260_residues
MKFLRSILVVALLQAAAATSALAADDLSQIKSAGVFRVGTEGTYAPFTYHDETGKLTGFDVDIATAIAQRLGVKPQFVEG
KWDGLIAGLDVNRYDAVVNEVSITDARKAKYDFSTPYITSHAVLIVRADNTTIRSFDDLKGKKSANTLTSNFGKLAAAHG
ADVVPVQGFNESIDLLTSGRVDATINDSLSYLDFRKHKPDAKLKIAATDTTGAGDASGVLLRKGSPALVAAIDKALADIK
ADGTYAKISQKYFGRDVSQP

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=259, Percent_Identity=38.996138996139, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=33.1877729257642, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1788649, Length=258, Percent_Identity=31.7829457364341, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787085, Length=253, Percent_Identity=31.2252964426877, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787088, Length=259, Percent_Identity=29.3436293436293, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787031, Length=258, Percent_Identity=30.2325581395349, Blast_Score=91, Evalue=8e-20,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27631; Mature: 27631

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLRSILVVALLQAAAATSALAADDLSQIKSAGVFRVGTEGTYAPFTYHDETGKLTGFD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEECCCCCEEECC
VDIATAIAQRLGVKPQFVEGKWDGLIAGLDVNRYDAVVNEVSITDARKAKYDFSTPYITS
HHHHHHHHHHHCCCCEEECCCCCCEEEECCCCHHHHHHHHEECCCCHHCCCCCCCCEEEC
HAVLIVRADNTTIRSFDDLKGKKSANTLTSNFGKLAAAHGADVVPVQGFNESIDLLTSGR
CEEEEEECCCCEECCHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEECCC
VDATINDSLSYLDFRKHKPDAKLKIAATDTTGAGDASGVLLRKGSPALVAAIDKALADIK
CCEEECCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHC
ADGTYAKISQKYFGRDVSQP
CCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKFLRSILVVALLQAAAATSALAADDLSQIKSAGVFRVGTEGTYAPFTYHDETGKLTGFD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEECCCCCEEECC
VDIATAIAQRLGVKPQFVEGKWDGLIAGLDVNRYDAVVNEVSITDARKAKYDFSTPYITS
HHHHHHHHHHHCCCCEEECCCCCCEEEECCCCHHHHHHHHEECCCCHHCCCCCCCCEEEC
HAVLIVRADNTTIRSFDDLKGKKSANTLTSNFGKLAAAHGADVVPVQGFNESIDLLTSGR
CEEEEEECCCCEECCHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEECCC
VDATINDSLSYLDFRKHKPDAKLKIAATDTTGAGDASGVLLRKGSPALVAAIDKALADIK
CCEEECCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHC
ADGTYAKISQKYFGRDVSQP
CCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]