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The map label for this gene is yafC [C]

Identifier: 116691149

GI number: 116691149

Start: 3448155

End: 3449204

Strand: Reverse

Name: yafC [C]

Synonym: Bcen2424_3131

Alternate gene names: 116691149

Gene position: 3449204-3448155 (Counterclockwise)

Preceding gene: 116691151

Following gene: 116691145

Centisome position: 99.0

GC content: 67.14

Gene sequence:

>1050_bases
ATGGATCATTTGCAGTCGATGCGCGTGTTCGTCAAGGTTGCAGATCTCGGCAGTTTCGCGCGGGCCGCGAGCGCAATGGA
TATCTCCAACGCGGTCGCGACGCGCCACGTCGCCGATCTGGAAGGCCGGCTCGGCACGCGTTTGCTTAACCGCACCACGC
GCAGCCTCTCCCTGACGGAGTCGGGTCAGGTCTATCTGGAGCGTGCCCGCCAGATCCTCGACGAGCTCGAGGATGTCGAG
CAGATGGTCGTCGCACGCAATCACGAGCCGGTCGGCACGCTGCGCATCGTCGCGCCGGTGGTGTTCGGCCTGCACAACCT
CGCCCCCGTGCTGCAGTCGTACACGGAGAATTTCCCGAAGGTGGTGCCCGATCTCACGCTGGTCGACCGGCAGGTCGATC
TGGTCGAGGAAGGTTTCGACGTCGGCATCGTCGTCACGCGCCAGATGCGTAGCGCGAGCATCGTCACGCGGCGCCTGACC
ACCGGTTGCATGACCGTGTGCGCGACGCCGAGCTATCTGGAAAAGCACGGCGTGCCGACGCATCCGGAACAGCTCGCCGA
GCATCCGGCGCTGAGCCTGCCGACCGAATACTGGGGCGACGAGCGCGTGTTCACGGGGCCGGACGGCGAAGTGCGCGTGC
GCCCGACCAACGTGATCGTCGCGAACAACACCGCGATGCTGCGCCAGTTCGCGTTGCTCGGGATGGGTGTCGCGATCCTG
CCGAGCTACCTGATCGGCAGCGACATCGCGCGCGGCGCGCTCGTGCGGCTGCTGCCGGATTTCCGGCTGCCGCAGGTCGA
GATCAACATCGCCTATCCGAGCCGGCGCCATCTGCCGGCGAAGGTGCGCACGTTCATCGACCATCTCGTCGAGCATTTCA
GCCACTCGACCGACGCGATGATGGGCGAACAGTGGGCCGCGCAGAGCGCGACGCTGCCGCCGATCGGCGTCGAGATGCGC
GCCGAGCAGCCCGAATCGACCGATCCGAACCTGCAGCCGCGTCTGGCGCGCTCGCCGCGCACGCGCGCAGCGGTGCCGTC
GCCGCTGTAA

Upstream 100 bases:

>100_bases
CCGGCCCCTTGTTGAGCAACTCTGCATCATTTCCGCTGTCGTAGGAGAATTACGCATTTGCTTCATCTTTTTGAAGTTGT
AACAGCGGCAGGAGTCGTTT

Downstream 100 bases:

>100_bases
CGGCGCTGCGGCCCGGGCCGGCAGCGCTACAGCCGGCACAAAGAAAAAACGCGGATCGATCGATCCGCGTTTTTTTATGG
CTGCGTGCCGGAGCCGGGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MDHLQSMRVFVKVADLGSFARAASAMDISNAVATRHVADLEGRLGTRLLNRTTRSLSLTESGQVYLERARQILDELEDVE
QMVVARNHEPVGTLRIVAPVVFGLHNLAPVLQSYTENFPKVVPDLTLVDRQVDLVEEGFDVGIVVTRQMRSASIVTRRLT
TGCMTVCATPSYLEKHGVPTHPEQLAEHPALSLPTEYWGDERVFTGPDGEVRVRPTNVIVANNTAMLRQFALLGMGVAIL
PSYLIGSDIARGALVRLLPDFRLPQVEINIAYPSRRHLPAKVRTFIDHLVEHFSHSTDAMMGEQWAAQSATLPPIGVEMR
AEQPESTDPNLQPRLARSPRTRAAVPSPL

Sequences:

>Translated_349_residues
MDHLQSMRVFVKVADLGSFARAASAMDISNAVATRHVADLEGRLGTRLLNRTTRSLSLTESGQVYLERARQILDELEDVE
QMVVARNHEPVGTLRIVAPVVFGLHNLAPVLQSYTENFPKVVPDLTLVDRQVDLVEEGFDVGIVVTRQMRSASIVTRRLT
TGCMTVCATPSYLEKHGVPTHPEQLAEHPALSLPTEYWGDERVFTGPDGEVRVRPTNVIVANNTAMLRQFALLGMGVAIL
PSYLIGSDIARGALVRLLPDFRLPQVEINIAYPSRRHLPAKVRTFIDHLVEHFSHSTDAMMGEQWAAQSATLPPIGVEMR
AEQPESTDPNLQPRLARSPRTRAAVPSPL
>Mature_349_residues
MDHLQSMRVFVKVADLGSFARAASAMDISNAVATRHVADLEGRLGTRLLNRTTRSLSLTESGQVYLERARQILDELEDVE
QMVVARNHEPVGTLRIVAPVVFGLHNLAPVLQSYTENFPKVVPDLTLVDRQVDLVEEGFDVGIVVTRQMRSASIVTRRLT
TGCMTVCATPSYLEKHGVPTHPEQLAEHPALSLPTEYWGDERVFTGPDGEVRVRPTNVIVANNTAMLRQFALLGMGVAIL
PSYLIGSDIARGALVRLLPDFRLPQVEINIAYPSRRHLPAKVRTFIDHLVEHFSHSTDAMMGEQWAAQSATLPPIGVEMR
AEQPESTDPNLQPRLARSPRTRAAVPSPL

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=33.5640138408304, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789639, Length=294, Percent_Identity=32.9931972789116, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=29.4520547945205, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI87081978, Length=303, Percent_Identity=31.3531353135314, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787128, Length=302, Percent_Identity=28.476821192053, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789440, Length=259, Percent_Identity=28.957528957529, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=29.6551724137931, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789173, Length=252, Percent_Identity=29.7619047619048, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1790011, Length=307, Percent_Identity=23.4527687296417, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787879, Length=263, Percent_Identity=27.7566539923954, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790262, Length=263, Percent_Identity=26.2357414448669, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788481, Length=289, Percent_Identity=23.5294117647059, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI157672245, Length=140, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788297, Length=143, Percent_Identity=29.3706293706294, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 38567; Mature: 38567

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHLQSMRVFVKVADLGSFARAASAMDISNAVATRHVADLEGRLGTRLLNRTTRSLSLTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
SGQVYLERARQILDELEDVEQMVVARNHEPVGTLRIVAPVVFGLHNLAPVLQSYTENFPK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVPDLTLVDRQVDLVEEGFDVGIVVTRQMRSASIVTRRLTTGCMTVCATPSYLEKHGVPT
HCCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
HPEQLAEHPALSLPTEYWGDERVFTGPDGEVRVRPTNVIVANNTAMLRQFALLGMGVAIL
CHHHHHCCCCCCCCHHHCCCCEEEECCCCCEEEEECEEEEECCHHHHHHHHHHHCCHHHH
PSYLIGSDIARGALVRLLPDFRLPQVEINIAYPSRRHLPAKVRTFIDHLVEHFSHSTDAM
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHH
MGEQWAAQSATLPPIGVEMRAEQPESTDPNLQPRLARSPRTRAAVPSPL
HHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MDHLQSMRVFVKVADLGSFARAASAMDISNAVATRHVADLEGRLGTRLLNRTTRSLSLTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
SGQVYLERARQILDELEDVEQMVVARNHEPVGTLRIVAPVVFGLHNLAPVLQSYTENFPK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVPDLTLVDRQVDLVEEGFDVGIVVTRQMRSASIVTRRLTTGCMTVCATPSYLEKHGVPT
HCCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
HPEQLAEHPALSLPTEYWGDERVFTGPDGEVRVRPTNVIVANNTAMLRQFALLGMGVAIL
CHHHHHCCCCCCCCHHHCCCCEEEECCCCCEEEEECEEEEECCHHHHHHHHHHHCCHHHH
PSYLIGSDIARGALVRLLPDFRLPQVEINIAYPSRRHLPAKVRTFIDHLVEHFSHSTDAM
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHH
MGEQWAAQSATLPPIGVEMRAEQPESTDPNLQPRLARSPRTRAAVPSPL
HHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]