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The map label for this gene is rsmB [H]

Identifier: 116691141

GI number: 116691141

Start: 3438445

End: 3439836

Strand: Reverse

Name: rsmB [H]

Synonym: Bcen2424_3123

Alternate gene names: 116691141

Gene position: 3439836-3438445 (Counterclockwise)

Preceding gene: 116691142

Following gene: 116691140

Centisome position: 98.74

GC content: 71.77

Gene sequence:

>1392_bases
ATGACACAACCCCGTTCTTCCGCTTCGACTTCTTCCGGCCGCCCGGCGCGCCTGGCTGCGCTGCATCTGGCGCCCGATTC
GCTCGGCTTCGCGCTCGACGCGGCCGCGCAGGCGGTCGACGCGGTGCGACGCGGCACCGCGTTGCCGGCGGCGCTGTCGA
CGGTATTCGCGCAGATGCCGTCCGGTGCGCAGGCGCTCGCGCGCGGCGCGACGCAGGACGTCGCCTACCGGACGATGCGT
CGCCTCGGCAGCGCGGACTGGCTGATCGGCAAGTTCGTCAGCAAGGCGCCGCCCGCGCACGTGCATGCGGTGCTCGCCGG
CGCGTTCGCGCTGCTGCTCGACCCGCCTGACGAGGCCGCGTACCCGGCGTTCACGGTGGTCGACCAGGCCGTCACCGTCA
TCGGTGCGCGCCGCGAATTCGCGTTCGCGAAGGGGATGGTCAACGCGGTGCTGCGCCGTTTCCTGCGCGAGCGCGACGCA
CTCGTCGCCGCGATGCAGGACGAGCCGGTCGCGCGCTGGAACTACCGCGCGTGGTGGGTTGATGCGGTCAAGCGCGCATG
GCCCGAGGCGTGGCAGGCGATCCTCGCGGCCGGCGAGCGTCAGGGGCCGCTGACGTTGCGCGTGAATGCGCGCCGCGCGA
GCGTCGATGCGTATCTCGACACGCTGCGCGCGAGCGGAATCGACGCGACCGCGATCGGCCGGCATGCAGTGCGGCTCGCG
TCGGCGCTGCCGGTGGAACGCATTCCGGGCTTCGCCGACGGCGTGGTGTCGGTGCAGGACGCCGGCGCGCAACTCGCGGC
CGAATGGCTCGACGCGCGCGACGGCATGCGCGTGCTCGACGCGTGCGCGGCGCCCGGCGGCAAGACGGGCCATATTCTCG
AACTCGCCGATGCGGAAGTCGTGGCGCTCGAAAGCGACGCGACGCGCGCGGCGCGCATCGGCGAGAATCTCGTGCGCCTG
TCGCTCGCGGCGAACGTGTGCGTCGGCGACGCGGGCGCGTCGGGCACCTGGTACGACGGGCGTCCGTTCGATCGCATCCT
GGCCGACGTGCCGTGCTCGGCGTCCGGCATCGTGCGGCGGCACCCCGACATCCGCTGGCTGCGCCGCGAGGCCGACATCC
CGGCGCTGGTCGCGGAGCAGCGTCGCATCCTGTCGGCGCTGTGGCCGCTCGTGAAGCCGGGCGGCGAACTGCTTTACGTG
ACCTGTTCGATCTTCCCCGAGGAAGGCGAATTGCAGGCCCGCTGGTTTGAAGCGGCCTGTGAAGATGCGGTACGATTGGA
CGCCCCCGGCCAACTGCTGCCGGGCAGCGTGCAGGGACGGGCGGCCGCCGGCGCACTCGACCAGAACACCGATCACGACG
GATTTTTCTACGCGCGGTTTCAGAAACGGTGA

Upstream 100 bases:

>100_bases
CGGTTTGGGATCGCCGGGTCATCTGGCCGCCGCGATCCCGCCGTTTGCCGCATTTGTTTGCCGCACCTGATTGCCGCGCC
TGCTTGCCCATTCCGCACCG

Downstream 100 bases:

>100_bases
CGATCAAACACCTTTTTCCCTTTCGGCTCGCGGCCGTCTTGCTGGTCGCGTTGACGCTGTGCCTGGCCGTCGTCCGGCCG
GCGCATGCCGAATCGATCGC

Product: sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTQPRSSASTSSGRPARLAALHLAPDSLGFALDAAAQAVDAVRRGTALPAALSTVFAQMPSGAQALARGATQDVAYRTMR
RLGSADWLIGKFVSKAPPAHVHAVLAGAFALLLDPPDEAAYPAFTVVDQAVTVIGARREFAFAKGMVNAVLRRFLRERDA
LVAAMQDEPVARWNYRAWWVDAVKRAWPEAWQAILAAGERQGPLTLRVNARRASVDAYLDTLRASGIDATAIGRHAVRLA
SALPVERIPGFADGVVSVQDAGAQLAAEWLDARDGMRVLDACAAPGGKTGHILELADAEVVALESDATRAARIGENLVRL
SLAANVCVGDAGASGTWYDGRPFDRILADVPCSASGIVRRHPDIRWLRREADIPALVAEQRRILSALWPLVKPGGELLYV
TCSIFPEEGELQARWFEAACEDAVRLDAPGQLLPGSVQGRAAAGALDQNTDHDGFFYARFQKR

Sequences:

>Translated_463_residues
MTQPRSSASTSSGRPARLAALHLAPDSLGFALDAAAQAVDAVRRGTALPAALSTVFAQMPSGAQALARGATQDVAYRTMR
RLGSADWLIGKFVSKAPPAHVHAVLAGAFALLLDPPDEAAYPAFTVVDQAVTVIGARREFAFAKGMVNAVLRRFLRERDA
LVAAMQDEPVARWNYRAWWVDAVKRAWPEAWQAILAAGERQGPLTLRVNARRASVDAYLDTLRASGIDATAIGRHAVRLA
SALPVERIPGFADGVVSVQDAGAQLAAEWLDARDGMRVLDACAAPGGKTGHILELADAEVVALESDATRAARIGENLVRL
SLAANVCVGDAGASGTWYDGRPFDRILADVPCSASGIVRRHPDIRWLRREADIPALVAEQRRILSALWPLVKPGGELLYV
TCSIFPEEGELQARWFEAACEDAVRLDAPGQLLPGSVQGRAAAGALDQNTDHDGFFYARFQKR
>Mature_462_residues
TQPRSSASTSSGRPARLAALHLAPDSLGFALDAAAQAVDAVRRGTALPAALSTVFAQMPSGAQALARGATQDVAYRTMRR
LGSADWLIGKFVSKAPPAHVHAVLAGAFALLLDPPDEAAYPAFTVVDQAVTVIGARREFAFAKGMVNAVLRRFLRERDAL
VAAMQDEPVARWNYRAWWVDAVKRAWPEAWQAILAAGERQGPLTLRVNARRASVDAYLDTLRASGIDATAIGRHAVRLAS
ALPVERIPGFADGVVSVQDAGAQLAAEWLDARDGMRVLDACAAPGGKTGHILELADAEVVALESDATRAARIGENLVRLS
LAANVCVGDAGASGTWYDGRPFDRILADVPCSASGIVRRHPDIRWLRREADIPALVAEQRRILSALWPLVKPGGELLYVT
CSIFPEEGELQARWFEAACEDAVRLDAPGQLLPGSVQGRAAAGALDQNTDHDGFFYARFQKR

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=299, Percent_Identity=28.7625418060201, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI76150623, Length=299, Percent_Identity=28.7625418060201, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI32698918, Length=222, Percent_Identity=30.6306306306306, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI301336155, Length=201, Percent_Identity=30.3482587064677, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI39995082, Length=201, Percent_Identity=30.3482587064677, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI2367212, Length=449, Percent_Identity=38.9755011135858, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI87081985, Length=290, Percent_Identity=31.7241379310345, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17536757, Length=269, Percent_Identity=29.368029739777, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324268, Length=298, Percent_Identity=29.1946308724832, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319447, Length=211, Percent_Identity=31.7535545023697, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI22024126, Length=250, Percent_Identity=27.6, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24668781, Length=239, Percent_Identity=33.8912133891213, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21356513, Length=234, Percent_Identity=33.7606837606838, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21355201, Length=217, Percent_Identity=31.7972350230415, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 49546; Mature: 49415

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPRSSASTSSGRPARLAALHLAPDSLGFALDAAAQAVDAVRRGTALPAALSTVFAQMP
CCCCCCCCCCCCCCCEEEEEEEECCCHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
SGAQALARGATQDVAYRTMRRLGSADWLIGKFVSKAPPAHVHAVLAGAFALLLDPPDEAA
CCHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCCCC
YPAFTVVDQAVTVIGARREFAFAKGMVNAVLRRFLRERDALVAAMQDEPVARWNYRAWWV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHH
DAVKRAWPEAWQAILAAGERQGPLTLRVNARRASVDAYLDTLRASGIDATAIGRHAVRLA
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SALPVERIPGFADGVVSVQDAGAQLAAEWLDARDGMRVLDACAAPGGKTGHILELADAEV
HHCCHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCEE
VALESDATRAARIGENLVRLSLAANVCVGDAGASGTWYDGRPFDRILADVPCSASGIVRR
EEECCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCCHHC
HPDIRWLRREADIPALVAEQRRILSALWPLVKPGGELLYVTCSIFPEEGELQARWFEAAC
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHH
EDAVRLDAPGQLLPGSVQGRAAAGALDQNTDHDGFFYARFQKR
HHCEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
TQPRSSASTSSGRPARLAALHLAPDSLGFALDAAAQAVDAVRRGTALPAALSTVFAQMP
CCCCCCCCCCCCCCEEEEEEEECCCHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
SGAQALARGATQDVAYRTMRRLGSADWLIGKFVSKAPPAHVHAVLAGAFALLLDPPDEAA
CCHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCCCC
YPAFTVVDQAVTVIGARREFAFAKGMVNAVLRRFLRERDALVAAMQDEPVARWNYRAWWV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHH
DAVKRAWPEAWQAILAAGERQGPLTLRVNARRASVDAYLDTLRASGIDATAIGRHAVRLA
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SALPVERIPGFADGVVSVQDAGAQLAAEWLDARDGMRVLDACAAPGGKTGHILELADAEV
HHCCHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCEE
VALESDATRAARIGENLVRLSLAANVCVGDAGASGTWYDGRPFDRILADVPCSASGIVRR
EEECCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCCHHC
HPDIRWLRREADIPALVAEQRRILSALWPLVKPGGELLYVTCSIFPEEGELQARWFEAAC
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHH
EDAVRLDAPGQLLPGSVQGRAAAGALDQNTDHDGFFYARFQKR
HHCEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA