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The map label for this gene is citA [H]

Identifier: 116691064

GI number: 116691064

Start: 3360563

End: 3361870

Strand: Reverse

Name: citA [H]

Synonym: Bcen2424_3046

Alternate gene names: 116691064

Gene position: 3361870-3360563 (Counterclockwise)

Preceding gene: 116691065

Following gene: 116691063

Centisome position: 96.5

GC content: 68.27

Gene sequence:

>1308_bases
ATGGCGACGATTGCGAGTTCAACGAAAACGACCCGGCCCGAGCGTGCGCTGAACCGGCGTGCGGTGGTGGCCGCCGTGAT
CGGCAATGCGCTCGAGTGGTACGACTTCACGGTATTCGGCTTCATGACGGTCGTGATCGCCGAGCTGTTCTTCCCCACCT
CCAGCGAATACTCGTCGCTGCTGCTCACCACCGCGACGTTCGGCGTCGCGTTCTTCATGCGCCCGATCGGCGGCATCGTG
TTCGGGCTGTACGCGGACCGCGCGGGCCGCAAGGCCGCGCTGTCGCTCGTGATCCTGCTGATGACGGCTGGCATCTTCCT
GCTCGCGATCGCGCCGCCCTATGCGGCGATCGGCATCGGCGGGCCGATCCTGATCGTGCTCGGCCGCCTGCTGCAGGGCT
TCTCCGCGGGCGGCGAATTCGGCAGCTCGACCGCGCTGCTGATCGAAGCGGCGCCGTTCTCGAAACGCGGTTTCTACGGC
AGCTGGCAGATGGCGAGCCAGGCGGCCGCGTTGCTGATCGGCGCGCTCGTCGGCGCGGCCGTCACGCGCGGGCTGTCGCA
CGACGCGCTGCGCAGCTGGGGCTGGCGCGTGCCGTTCATCCTCGGGCTCGTGATCGGCCCGATCGGCTTCTACATCCGCC
GCCATCTCGCCGATTCCGAAGCGTTCCTGCATGCGCAGCAGAGCGCGCGCCGCGCGACGCTCGGCGAAGTGTTCACGCGC
CACCCGCGCGAGGTGCTGTGCGGGCTCGGCTCGGTGATCGCGCTCACCGTGACGATCTACGTGCTGATCAGCTATCTGCC
GACCTTCGCAGTGAAGCAGCTGAAGCTGCCGTATGCGGAATCGTTCTACGCGGTGATCGTCGGCAACCTGCTGCTGATGG
TGCTGTCGCCGGTCGCGGGCGCGTGGTCGGACCGTATCGGCCGCAAGGGCCTGTCGCTGTGGTCGCTGGTGCTGACGCTC
GCGCTGATGTATCCGCTGTTCGCGTGGCTCGACGCCGCGCCGAGCATCGGGCGGCTGATCGCGGTACAGGCCGTGCTGTC
GGTGACGCTGGCAGGCTACTACGGGCCGTTCGGCGCGATGATCGCCGAGCTGTTCCCGGCCAACGTGCGCTCGACCGGGC
TGTCGATCGCGTACAACGTCGCGGTGATGCTGTTCGGCGGCTTCGGCCAGTTCATCGTCACGTGGCTGATCAAGGTGACC
GGCACGCCGCTCGCGCCGACCTACTACGTGATGGCGGGGATCGCGCTGTCGATCGTGGCGGTCGCGTTCGTGCCCGCGCG
CAACGCCGACCTCGATGCGCCGGCGTGA

Upstream 100 bases:

>100_bases
AACGGTTCCGTCGCTTTCTACACTCGCTGCACACCAGCGCGCCGCGGCACCCCGCGCGCCGCACCGCCCGACACGGCGAA
TTCACGAGGACGAGGCACGC

Downstream 100 bases:

>100_bases
CGACATGACGATGTGAAGACGGTCATCGAATCAATCGTTTCGCGAAACGCAATGATGTGCTGCGCAACGCGGGCAAACGG
TGCGTCGCCCCTTCGTGTTC

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MATIASSTKTTRPERALNRRAVVAAVIGNALEWYDFTVFGFMTVVIAELFFPTSSEYSSLLLTTATFGVAFFMRPIGGIV
FGLYADRAGRKAALSLVILLMTAGIFLLAIAPPYAAIGIGGPILIVLGRLLQGFSAGGEFGSSTALLIEAAPFSKRGFYG
SWQMASQAAALLIGALVGAAVTRGLSHDALRSWGWRVPFILGLVIGPIGFYIRRHLADSEAFLHAQQSARRATLGEVFTR
HPREVLCGLGSVIALTVTIYVLISYLPTFAVKQLKLPYAESFYAVIVGNLLLMVLSPVAGAWSDRIGRKGLSLWSLVLTL
ALMYPLFAWLDAAPSIGRLIAVQAVLSVTLAGYYGPFGAMIAELFPANVRSTGLSIAYNVAVMLFGGFGQFIVTWLIKVT
GTPLAPTYYVMAGIALSIVAVAFVPARNADLDAPA

Sequences:

>Translated_435_residues
MATIASSTKTTRPERALNRRAVVAAVIGNALEWYDFTVFGFMTVVIAELFFPTSSEYSSLLLTTATFGVAFFMRPIGGIV
FGLYADRAGRKAALSLVILLMTAGIFLLAIAPPYAAIGIGGPILIVLGRLLQGFSAGGEFGSSTALLIEAAPFSKRGFYG
SWQMASQAAALLIGALVGAAVTRGLSHDALRSWGWRVPFILGLVIGPIGFYIRRHLADSEAFLHAQQSARRATLGEVFTR
HPREVLCGLGSVIALTVTIYVLISYLPTFAVKQLKLPYAESFYAVIVGNLLLMVLSPVAGAWSDRIGRKGLSLWSLVLTL
ALMYPLFAWLDAAPSIGRLIAVQAVLSVTLAGYYGPFGAMIAELFPANVRSTGLSIAYNVAVMLFGGFGQFIVTWLIKVT
GTPLAPTYYVMAGIALSIVAVAFVPARNADLDAPA
>Mature_434_residues
ATIASSTKTTRPERALNRRAVVAAVIGNALEWYDFTVFGFMTVVIAELFFPTSSEYSSLLLTTATFGVAFFMRPIGGIVF
GLYADRAGRKAALSLVILLMTAGIFLLAIAPPYAAIGIGGPILIVLGRLLQGFSAGGEFGSSTALLIEAAPFSKRGFYGS
WQMASQAAALLIGALVGAAVTRGLSHDALRSWGWRVPFILGLVIGPIGFYIRRHLADSEAFLHAQQSARRATLGEVFTRH
PREVLCGLGSVIALTVTIYVLISYLPTFAVKQLKLPYAESFYAVIVGNLLLMVLSPVAGAWSDRIGRKGLSLWSLVLTLA
LMYPLFAWLDAAPSIGRLIAVQAVLSVTLAGYYGPFGAMIAELFPANVRSTGLSIAYNVAVMLFGGFGQFIVTWLIKVTG
TPLAPTYYVMAGIALSIVAVAFVPARNADLDAPA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=406, Percent_Identity=32.7586206896552, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788942, Length=416, Percent_Identity=32.2115384615385, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789941, Length=415, Percent_Identity=33.0120481927711, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1788292, Length=432, Percent_Identity=29.6296296296296, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI87082231, Length=248, Percent_Identity=27.8225806451613, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87082404, Length=429, Percent_Identity=23.7762237762238, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46302; Mature: 46171

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIASSTKTTRPERALNRRAVVAAVIGNALEWYDFTVFGFMTVVIAELFFPTSSEYSSL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LLTTATFGVAFFMRPIGGIVFGLYADRAGRKAALSLVILLMTAGIFLLAIAPPYAAIGIG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
GPILIVLGRLLQGFSAGGEFGSSTALLIEAAPFSKRGFYGSWQMASQAAALLIGALVGAA
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VTRGLSHDALRSWGWRVPFILGLVIGPIGFYIRRHLADSEAFLHAQQSARRATLGEVFTR
HHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
HPREVLCGLGSVIALTVTIYVLISYLPTFAVKQLKLPYAESFYAVIVGNLLLMVLSPVAG
CHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AWSDRIGRKGLSLWSLVLTLALMYPLFAWLDAAPSIGRLIAVQAVLSVTLAGYYGPFGAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHH
IAELFPANVRSTGLSIAYNVAVMLFGGFGQFIVTWLIKVTGTPLAPTYYVMAGIALSIVA
HHHHCCCCHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VAFVPARNADLDAPA
HHHHCCCCCCCCCCC
>Mature Secondary Structure 
ATIASSTKTTRPERALNRRAVVAAVIGNALEWYDFTVFGFMTVVIAELFFPTSSEYSSL
CCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LLTTATFGVAFFMRPIGGIVFGLYADRAGRKAALSLVILLMTAGIFLLAIAPPYAAIGIG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
GPILIVLGRLLQGFSAGGEFGSSTALLIEAAPFSKRGFYGSWQMASQAAALLIGALVGAA
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VTRGLSHDALRSWGWRVPFILGLVIGPIGFYIRRHLADSEAFLHAQQSARRATLGEVFTR
HHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
HPREVLCGLGSVIALTVTIYVLISYLPTFAVKQLKLPYAESFYAVIVGNLLLMVLSPVAG
CHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AWSDRIGRKGLSLWSLVLTLALMYPLFAWLDAAPSIGRLIAVQAVLSVTLAGYYGPFGAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHH
IAELFPANVRSTGLSIAYNVAVMLFGGFGQFIVTWLIKVTGTPLAPTYYVMAGIALSIVA
HHHHCCCCHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VAFVPARNADLDAPA
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]