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The map label for this gene is potF [H]

Identifier: 116691014

GI number: 116691014

Start: 3307076

End: 3308191

Strand: Direct

Name: potF [H]

Synonym: Bcen2424_2996

Alternate gene names: 116691014

Gene position: 3307076-3308191 (Clockwise)

Preceding gene: 116691013

Following gene: 116691015

Centisome position: 94.92

GC content: 62.99

Gene sequence:

>1116_bases
ATGCGTGCCTGCATTCTTCGCCAAGCCTGTTCCGTCGCCGCCCTCGCCGCCGCGGCCGCGCTCACGTCGGTCGCCAGCCC
GTCGGCGCATGCCGACGAGCTGAACGTCTACAACTGGTCCGACTACATCGCGCCGGACACGATCCCGAACTTCCAGAAGC
AGACGGGTATTCACGTCAAGTACGACAACTACGACAGCGACGACACGCTCCAGGCGAAGCTGCTCGCCGGCAGTTCCGGC
TACGACATCGTCGTGCCGACCTCGAACTACATGGCCAAGCAGATCCAGGCCGGCGTGTACCAGAAGCTCGACAAGTCGAA
GATTCCGAACCTCGCGAACCTCGACCCGCTGCTGATGAAGATGATCTCGGATGCCGATCCGGGCAACCAGTACGGCGTGC
CCTGGGCGTACGGCACGGACGGCATCGGCTACAACGCGCAGGCGGTGAAGAAGGCGCTCGGCGACAAGGCGCCGGTCGAC
AGCTGGGCGCTGGTGTTCGATCCGGCCAACATGGAGAAGCTGAAGAGCTGCGGCGTGTCGTTCCTCGACCAGGCCGTCGA
CGTGTTCGCCGCCACGCTGCAATACATGGGCAAGGACCCGAACAGCAAGAACCCCGGCGACTACCAGGCCGCATTCGAAG
TCCTGAAGAAAGTCCGCCCGTACATCACCCAGTTCAACTCGTCCGGCTACATCAACGACCTCGCGAACAACGACGTGTGC
GTCGTGCTCGGCTGGTCCGGCGACGTCGGCATCGCGCACCGTCGCTCGGCGGAAGCGAAGCGTTCGTACGACATCAAGTT
CTCGAATCCGAAGGAAGGCGGCCTGCTGTGGTTCGACGTGATGGTGATCCCGAAGGATGCGCCGCATCCCGAGAGCGCGC
TGAAGTGGATCAACTACATTTCCGATCCGAAGGTCAACGCGGCGATCACCAACACGGTGTTCTACCCGACCGCGAACAAG
GCCGCGCATCAATTCGTCACGCCGGCCGTCGCGCAGGACCCGACCGTCTATCCGCCTGAAGACGTGCTGAAGAAGATGGT
GCTGATGAAGCCGATGCCGGCCGACATCCTGCGCCTCGAGAACCGTCTGTGGGCCCAGTTGAAGACGGGCCACTGA

Upstream 100 bases:

>100_bases
CCGTCGATGCCACCGCCCGGCAACTGGGCGTTTCGTAACGGTTTTGCCTACAGGCGTGCGGCGGGCGCCGCACGCCGCCA
TCACTCAAGGGAATTCCACC

Downstream 100 bases:

>100_bases
TCGCCATGCATCACGGCGGCGCAGGCATCGCGGTACGCCTGCCCGCCTGAAATTCGCGGACCTCTGTCCGCTCGCGCGCA
CCGCGCGCCCTGTTCCATCC

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MRACILRQACSVAALAAAAALTSVASPSAHADELNVYNWSDYIAPDTIPNFQKQTGIHVKYDNYDSDDTLQAKLLAGSSG
YDIVVPTSNYMAKQIQAGVYQKLDKSKIPNLANLDPLLMKMISDADPGNQYGVPWAYGTDGIGYNAQAVKKALGDKAPVD
SWALVFDPANMEKLKSCGVSFLDQAVDVFAATLQYMGKDPNSKNPGDYQAAFEVLKKVRPYITQFNSSGYINDLANNDVC
VVLGWSGDVGIAHRRSAEAKRSYDIKFSNPKEGGLLWFDVMVIPKDAPHPESALKWINYISDPKVNAAITNTVFYPTANK
AAHQFVTPAVAQDPTVYPPEDVLKKMVLMKPMPADILRLENRLWAQLKTGH

Sequences:

>Translated_371_residues
MRACILRQACSVAALAAAAALTSVASPSAHADELNVYNWSDYIAPDTIPNFQKQTGIHVKYDNYDSDDTLQAKLLAGSSG
YDIVVPTSNYMAKQIQAGVYQKLDKSKIPNLANLDPLLMKMISDADPGNQYGVPWAYGTDGIGYNAQAVKKALGDKAPVD
SWALVFDPANMEKLKSCGVSFLDQAVDVFAATLQYMGKDPNSKNPGDYQAAFEVLKKVRPYITQFNSSGYINDLANNDVC
VVLGWSGDVGIAHRRSAEAKRSYDIKFSNPKEGGLLWFDVMVIPKDAPHPESALKWINYISDPKVNAAITNTVFYPTANK
AAHQFVTPAVAQDPTVYPPEDVLKKMVLMKPMPADILRLENRLWAQLKTGH
>Mature_371_residues
MRACILRQACSVAALAAAAALTSVASPSAHADELNVYNWSDYIAPDTIPNFQKQTGIHVKYDNYDSDDTLQAKLLAGSSG
YDIVVPTSNYMAKQIQAGVYQKLDKSKIPNLANLDPLLMKMISDADPGNQYGVPWAYGTDGIGYNAQAVKKALGDKAPVD
SWALVFDPANMEKLKSCGVSFLDQAVDVFAATLQYMGKDPNSKNPGDYQAAFEVLKKVRPYITQFNSSGYINDLANNDVC
VVLGWSGDVGIAHRRSAEAKRSYDIKFSNPKEGGLLWFDVMVIPKDAPHPESALKWINYISDPKVNAAITNTVFYPTANK
AAHQFVTPAVAQDPTVYPPEDVLKKMVLMKPMPADILRLENRLWAQLKTGH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=357, Percent_Identity=49.2997198879552, Blast_Score=370, Evalue=1e-103,
Organism=Escherichia coli, GI1787367, Length=321, Percent_Identity=36.4485981308411, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1787711, Length=321, Percent_Identity=25.2336448598131, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40612; Mature: 40612

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRACILRQACSVAALAAAAALTSVASPSAHADELNVYNWSDYIAPDTIPNFQKQTGIHVK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCHHHHCCCEEE
YDNYDSDDTLQAKLLAGSSGYDIVVPTSNYMAKQIQAGVYQKLDKSKIPNLANLDPLLMK
ECCCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
MISDADPGNQYGVPWAYGTDGIGYNAQAVKKALGDKAPVDSWALVFDPANMEKLKSCGVS
HHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHCCHH
FLDQAVDVFAATLQYMGKDPNSKNPGDYQAAFEVLKKVRPYITQFNSSGYINDLANNDVC
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEE
VVLGWSGDVGIAHRRSAEAKRSYDIKFSNPKEGGLLWFDVMVIPKDAPHPESALKWINYI
EEEECCCCCCCCCCCCCHHHHCCCEEECCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHH
SDPKVNAAITNTVFYPTANKAAHQFVTPAVAQDPTVYPPEDVLKKMVLMKPMPADILRLE
CCCCCCEEEECEEEECCCCHHHHHHHCCHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
NRLWAQLKTGH
HHHHHHCCCCC
>Mature Secondary Structure
MRACILRQACSVAALAAAAALTSVASPSAHADELNVYNWSDYIAPDTIPNFQKQTGIHVK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCHHHHCCCEEE
YDNYDSDDTLQAKLLAGSSGYDIVVPTSNYMAKQIQAGVYQKLDKSKIPNLANLDPLLMK
ECCCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
MISDADPGNQYGVPWAYGTDGIGYNAQAVKKALGDKAPVDSWALVFDPANMEKLKSCGVS
HHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHCCHH
FLDQAVDVFAATLQYMGKDPNSKNPGDYQAAFEVLKKVRPYITQFNSSGYINDLANNDVC
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEE
VVLGWSGDVGIAHRRSAEAKRSYDIKFSNPKEGGLLWFDVMVIPKDAPHPESALKWINYI
EEEECCCCCCCCCCCCCHHHHCCCEEECCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHH
SDPKVNAAITNTVFYPTANKAAHQFVTPAVAQDPTVYPPEDVLKKMVLMKPMPADILRLE
CCCCCCEEEECEEEECCCCHHHHHHHCCHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
NRLWAQLKTGH
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]