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The map label for this gene is ttcA [H]

Identifier: 116690998

GI number: 116690998

Start: 3292527

End: 3293522

Strand: Direct

Name: ttcA [H]

Synonym: Bcen2424_2980

Alternate gene names: 116690998

Gene position: 3292527-3293522 (Clockwise)

Preceding gene: 116690997

Following gene: 116690999

Centisome position: 94.51

GC content: 65.76

Gene sequence:

>996_bases
ATGAACGCCCCCCACATGAACGACACGGCGGCCGACGCCGCCACGCTGGACGACGCCGCCGCGCCGGCCGGCCGCCCCGC
GCTGACGCGCCGCGAGCAGAAGGAAGCGTACGAGAACAACAAGCTGTTCAAGCGGATCGTGCGCCAGGTCGGCCAGGCGA
TCGGCGACTACAACATGATCGAGCAGGGCGACAAGGTGATGGTGTGCCTGTCGGGCGGCAAGGACAGTTACGCGATGCTC
GACGTGCTGCTGCGCCTGCGCGAGCGCGCGCCGATCGACTTCGACATCGTCGCGGTGAACCTCGACCAGAAGCAGCCGGG
CTTCCCCGAGCACGTACTGCCCGAATACCTGAAGCAGGTCGGCGTGCCGTTCCACATCGAGAACCAGGACACCTACAGCA
TCGTCAAGCGGCTCGTGCCGGAAGGCAAGACGACCTGCTCGCTGTGCTCGCGGCTGCGCCGCGGCATCCTGTACCGCGTG
GCCGGCGAGCTCGGCGCGACCAAGATCGCGCTCGGCCATCATCGCGACGACATCGTGCAGACGCTGCTGCTGAACATGTT
CTACGGCGGCAAGCTGAAGGGAATGCCGCCGAAGCTGCAGTCGGACGACGGCAAGAACATCGTGATCCGCCCGCTCGCGT
ACGTGAAGGAAACCGATCTCGAGAAATACGCGGAGCTGCGCGAATTCCCGATCATCCCGTGCAACCTGTGCGGCAGCCAG
CCGAACCTGAAGCGCGCGGAAATGAAGGCGCTGATCCGCGAATGGGACAAACGCTTCCCGGGCCGCGTCGACAACATGTT
CAACGCGCTCGCGAAGGTCGTGCCGTCGCACCTGATGGACACGACGCTGTACCCGTTCCAGTCGCTGCGCGCGACGGGTG
AAGCCGATCCGCAAGGCGACATCGCGTTCGACGAGGAACCGTGCGCATCGGGTGACGACGCGGCTGCGCCGGGCGCCGCC
CAACCGATCTCGATCGTCCAGTTCGACGACCTGTAA

Upstream 100 bases:

>100_bases
CCGTGCGCGCGGTACGTGTCCGTACCGAGAAACCGGACGCCTATCCGGACTGCGACGCCGTCGGCGTCGAAGTCTTTCGC
ATCAAGGACGAGGAGCGCCA

Downstream 100 bases:

>100_bases
GCAGGGCCGGAACACTCGCCGAAACCGGGGCCGGCGGCCTCGTTTCGGCGTTGTCGCCGCGCCGCGACAGGCCCGGAGCG
ACAAGGGCCGCATGCTAAAA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNAPHMNDTAADAATLDDAAAPAGRPALTRREQKEAYENNKLFKRIVRQVGQAIGDYNMIEQGDKVMVCLSGGKDSYAML
DVLLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLKQVGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRV
AGELGATKIALGHHRDDIVQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYVKETDLEKYAELREFPIIPCNLCGSQ
PNLKRAEMKALIREWDKRFPGRVDNMFNALAKVVPSHLMDTTLYPFQSLRATGEADPQGDIAFDEEPCASGDDAAAPGAA
QPISIVQFDDL

Sequences:

>Translated_331_residues
MNAPHMNDTAADAATLDDAAAPAGRPALTRREQKEAYENNKLFKRIVRQVGQAIGDYNMIEQGDKVMVCLSGGKDSYAML
DVLLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLKQVGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRV
AGELGATKIALGHHRDDIVQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYVKETDLEKYAELREFPIIPCNLCGSQ
PNLKRAEMKALIREWDKRFPGRVDNMFNALAKVVPSHLMDTTLYPFQSLRATGEADPQGDIAFDEEPCASGDDAAAPGAA
QPISIVQFDDL
>Mature_331_residues
MNAPHMNDTAADAATLDDAAAPAGRPALTRREQKEAYENNKLFKRIVRQVGQAIGDYNMIEQGDKVMVCLSGGKDSYAML
DVLLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLKQVGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRV
AGELGATKIALGHHRDDIVQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYVKETDLEKYAELREFPIIPCNLCGSQ
PNLKRAEMKALIREWDKRFPGRVDNMFNALAKVVPSHLMDTTLYPFQSLRATGEADPQGDIAFDEEPCASGDDAAAPGAA
QPISIVQFDDL

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=274, Percent_Identity=66.7883211678832, Blast_Score=403, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI193211090, Length=243, Percent_Identity=30.8641975308642, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17540090, Length=270, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24651972, Length=278, Percent_Identity=28.4172661870504, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 36738; Mature: 36738

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPHMNDTAADAATLDDAAAPAGRPALTRREQKEAYENNKLFKRIVRQVGQAIGDYNMI
CCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EQGDKVMVCLSGGKDSYAMLDVLLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLKQV
CCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHC
GVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRVAGELGATKIALGHHRDDIVQ
CCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
TLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYVKETDLEKYAELREFPIIPCNLCGSQ
HHHHHHHCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHCCCCC
PNLKRAEMKALIREWDKRFPGRVDNMFNALAKVVPSHLMDTTLYPFQSLRATGEADPQGD
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
IAFDEEPCASGDDAAAPGAAQPISIVQFDDL
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MNAPHMNDTAADAATLDDAAAPAGRPALTRREQKEAYENNKLFKRIVRQVGQAIGDYNMI
CCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EQGDKVMVCLSGGKDSYAMLDVLLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLKQV
CCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHC
GVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRVAGELGATKIALGHHRDDIVQ
CCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
TLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYVKETDLEKYAELREFPIIPCNLCGSQ
HHHHHHHCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHCCCCC
PNLKRAEMKALIREWDKRFPGRVDNMFNALAKVVPSHLMDTTLYPFQSLRATGEADPQGD
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
IAFDEEPCASGDDAAAPGAAQPISIVQFDDL
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA