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The map label for this gene is yfcG [H]

Identifier: 116690961

GI number: 116690961

Start: 3256173

End: 3256787

Strand: Direct

Name: yfcG [H]

Synonym: Bcen2424_2943

Alternate gene names: 116690961

Gene position: 3256173-3256787 (Clockwise)

Preceding gene: 116690959

Following gene: 116690964

Centisome position: 93.46

GC content: 69.27

Gene sequence:

>615_bases
ATGAAGCTCTACTACTGGCCGAAGACCCGGGCATTCCGGGCGCTGTGGATGCTCGAGGAAATCGGCGTGGTGTACGAACT
CGTGCCGATCGACCTGCGCTCGCACGAACAGGGCAGCGACGCGTTCGTGCAGGTCAACCCGATGGCGAAGCTGCCCGCGC
TCGACGACGGCAGCGCCCCGTTCGCCGAATCGGGCGCCGTGCTGCTCTATCTTGCCGACCGTTGCCCGGGCGCCGGGCTC
GGCGTCGCGCCCGACGATCCGCTGCGCGGCCGCTTCCTCCAGTGGATGTTCTTCACGCCGACCTGCCTCGAACCGGCGAT
GGCCGAGAAATTCACCGGTGCGTCGGGCAATCCGGTCGCGTTCGGCTGGGGCAATATCACGCGCGTGCAGCGCGCCCTCG
CGCAAGCGCTCGCCCACAGCCGCTGGCTCGTCGGCGAACGCTTCACCGCCGCCGACCTGCTGCTCGCCAGCACGCTGAAG
ATCGCGTTCGATGCGCATCTGCTGCCGCACGAAGGCGTGCTCGGCGACTACGTCGCACGCGCCGAGGACCGCGACGCGTT
CCGCCGCGCGGTCGCGATCGAACAGCGCGAAGCGGCGCGTCTGCTGCACGCATGA

Upstream 100 bases:

>100_bases
GCCCTGTGCCCCTAGCCGGGCGCCCCTGTCCCAACCTCCCGCGCTCGGGTACGCTTGAGCATCGATCGGATCGACAGCAG
CACCCAAACGCGGAGGCGCG

Downstream 100 bases:

>100_bases
CGCGCGACGGGGCGGCGCCCCGCCGCCCTTGATCACGCCTGCGCGGCCGGCCCCGGCGCCGACGCACCCGCGTCGATCAC
CACGCCCGGCGAACCGCGCT

Product: glutathione S-transferase domain-containing protein

Products: NA

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MKLYYWPKTRAFRALWMLEEIGVVYELVPIDLRSHEQGSDAFVQVNPMAKLPALDDGSAPFAESGAVLLYLADRCPGAGL
GVAPDDPLRGRFLQWMFFTPTCLEPAMAEKFTGASGNPVAFGWGNITRVQRALAQALAHSRWLVGERFTAADLLLASTLK
IAFDAHLLPHEGVLGDYVARAEDRDAFRRAVAIEQREAARLLHA

Sequences:

>Translated_204_residues
MKLYYWPKTRAFRALWMLEEIGVVYELVPIDLRSHEQGSDAFVQVNPMAKLPALDDGSAPFAESGAVLLYLADRCPGAGL
GVAPDDPLRGRFLQWMFFTPTCLEPAMAEKFTGASGNPVAFGWGNITRVQRALAQALAHSRWLVGERFTAADLLLASTLK
IAFDAHLLPHEGVLGDYVARAEDRDAFRRAVAIEQREAARLLHA
>Mature_204_residues
MKLYYWPKTRAFRALWMLEEIGVVYELVPIDLRSHEQGSDAFVQVNPMAKLPALDDGSAPFAESGAVLLYLADRCPGAGL
GVAPDDPLRGRFLQWMFFTPTCLEPAMAEKFTGASGNPVAFGWGNITRVQRALAQALAHSRWLVGERFTAADLLLASTLK
IAFDAHLLPHEGVLGDYVARAEDRDAFRRAVAIEQREAARLLHA

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=166, Percent_Identity=28.9156626506024, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI1787923, Length=202, Percent_Identity=28.2178217821782, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 22523; Mature: 22523

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYYWPKTRAFRALWMLEEIGVVYELVPIDLRSHEQGSDAFVQVNPMAKLPALDDGSAP
CEEEECCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
FAESGAVLLYLADRCPGAGLGVAPDDPLRGRFLQWMFFTPTCLEPAMAEKFTGASGNPVA
CCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHEEEEECCCHHCCHHHHHHCCCCCCCEEE
FGWGNITRVQRALAQALAHSRWLVGERFTAADLLLASTLKIAFDAHLLPHEGVLGDYVAR
ECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
AEDRDAFRRAVAIEQREAARLLHA
CCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLYYWPKTRAFRALWMLEEIGVVYELVPIDLRSHEQGSDAFVQVNPMAKLPALDDGSAP
CEEEECCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
FAESGAVLLYLADRCPGAGLGVAPDDPLRGRFLQWMFFTPTCLEPAMAEKFTGASGNPVA
CCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHEEEEECCCHHCCHHHHHHCCCCCCCEEE
FGWGNITRVQRALAQALAHSRWLVGERFTAADLLLASTLKIAFDAHLLPHEGVLGDYVAR
ECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
AEDRDAFRRAVAIEQREAARLLHA
CCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]