Click here to switch to the map view.

The map label for this gene is bioB [H]

Identifier: 116690952

GI number: 116690952

Start: 3243449

End: 3244468

Strand: Direct

Name: bioB [H]

Synonym: Bcen2424_2934

Alternate gene names: 116690952

Gene position: 3243449-3244468 (Clockwise)

Preceding gene: 116690951

Following gene: 116690957

Centisome position: 93.1

GC content: 68.24

Gene sequence:

>1020_bases
ATGACCCAAGCCCAGACCGCCGCCGTGCAACCCGCCGCGATCCCCGTGGCCGCCCCGGCGTCGCAGCGCTGGCGCGTCGC
CGACGTCGTCGCGCTGTTCGAGCTGCCGTTCAACGACCTGATGTTCCGCGCGCAGCAGGTGCATCGCGAGCACTTCGACG
CGAACGCGGTGCAGTTGTCGACGCTGCTGTCGATCAAGACGGGCGGCTGCGAGGAAGATTGCGGCTACTGCTCGCAGTCG
TCGCATCACGACACGGGCCTGAAGGCCGAGAAGCTGATGGACGTCGACACGGTGCTCGACGCCGCGCGCGCGGCGAAGGC
GAACGGCGCGAGCCGCTTCTGCATGGGCGCCGCGTGGCGCAACCCGAAGGAGCGCCACATGCCGGCGCTGACCGAGATGG
TGCGCGGTGTGAAGGAGCTCGGTCTCGAGACCTGCATGACGCTCGGCATGCTCGAAGACGAACAGGCGCAACAGCTCGCC
GATGCGGGCCTCGACTACTACAACCACAACCTCGACACGTCGCCGGAGTTCTACGGCCAGGTGATCTCGACGCGCACGTA
CCAGGATCGCCTCGACACGCTCGACCGCGTGCGCGATGCGGGCATCAACGTGTGCTGCGGCGGCATCATCGGGATGGGCG
AGTCGCGCCGCGAACGCGCGGGCCTGATCTCGCAGCTCGCGAACCTGAACCCGTATCCGGAATCGGTGCCGATCAACAAC
CTCGTCGCGATCGAAGGCACGCCGCTCGAAGGCACCGCGCCGCTCGATCCGTTCGAATTCGTGCGCACCATCGCGGTCGC
GCGCATCACGATGCCGAAGGCCGTCGTGCGCCTGTCGGCCGGCCGCGAGCAGCTCGACGACGCGATGCAGGCGATGTGTT
TCCTCGCCGGCGCCAACTCGATGTTCTACGGCGACCAGTTGCTGACGACCAGCAACCCGCAGACGCAGCGCGACCGCGCG
CTGTTCGAGCGCCTCGGCATCCGCGCGAGCCAGGCCGACGCGCTGTCGGACAACGCGTAA

Upstream 100 bases:

>100_bases
CCGCGGCGATGCTCGACATCGCCGCGCTCGTCGAGTCGCTGCGCACCGCGCGGCGTTGACCCAACCGATCCCCCAATCCC
GCCCAGGACAGGAAAACGAA

Downstream 100 bases:

>100_bases
GCGGCAACGGCCGGCGCACCGCGCCCGCCAACGAAAAAGCCGGGGCATGCCCCGGCTTTTTTACGTCCAATCGTCTGGAT
CGGGTCGCCCCTGCGCGTCG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTQAQTAAVQPAAIPVAAPASQRWRVADVVALFELPFNDLMFRAQQVHREHFDANAVQLSTLLSIKTGGCEEDCGYCSQS
SHHDTGLKAEKLMDVDTVLDAARAAKANGASRFCMGAAWRNPKERHMPALTEMVRGVKELGLETCMTLGMLEDEQAQQLA
DAGLDYYNHNLDTSPEFYGQVISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPESVPINN
LVAIEGTPLEGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDAMQAMCFLAGANSMFYGDQLLTTSNPQTQRDRA
LFERLGIRASQADALSDNA

Sequences:

>Translated_339_residues
MTQAQTAAVQPAAIPVAAPASQRWRVADVVALFELPFNDLMFRAQQVHREHFDANAVQLSTLLSIKTGGCEEDCGYCSQS
SHHDTGLKAEKLMDVDTVLDAARAAKANGASRFCMGAAWRNPKERHMPALTEMVRGVKELGLETCMTLGMLEDEQAQQLA
DAGLDYYNHNLDTSPEFYGQVISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPESVPINN
LVAIEGTPLEGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDAMQAMCFLAGANSMFYGDQLLTTSNPQTQRDRA
LFERLGIRASQADALSDNA
>Mature_338_residues
TQAQTAAVQPAAIPVAAPASQRWRVADVVALFELPFNDLMFRAQQVHREHFDANAVQLSTLLSIKTGGCEEDCGYCSQSS
HHDTGLKAEKLMDVDTVLDAARAAKANGASRFCMGAAWRNPKERHMPALTEMVRGVKELGLETCMTLGMLEDEQAQQLAD
AGLDYYNHNLDTSPEFYGQVISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPESVPINNL
VAIEGTPLEGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDAMQAMCFLAGANSMFYGDQLLTTSNPQTQRDRAL
FERLGIRASQADALSDNA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=317, Percent_Identity=64.3533123028391, Blast_Score=439, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6321725, Length=339, Percent_Identity=47.4926253687316, Blast_Score=331, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37014; Mature: 36883

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAQTAAVQPAAIPVAAPASQRWRVADVVALFELPFNDLMFRAQQVHREHFDANAVQLS
CCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
TLLSIKTGGCEEDCGYCSQSSHHDTGLKAEKLMDVDTVLDAARAAKANGASRFCMGAAWR
HHHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
NPKERHMPALTEMVRGVKELGLETCMTLGMLEDEQAQQLADAGLDYYNHNLDTSPEFYGQ
CCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHCCCCCCCHHHHHH
VISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPESVPINN
HHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
LVAIEGTPLEGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDAMQAMCFLAGANS
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
MFYGDQLLTTSNPQTQRDRALFERLGIRASQADALSDNA
CEECCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQAQTAAVQPAAIPVAAPASQRWRVADVVALFELPFNDLMFRAQQVHREHFDANAVQLS
CCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
TLLSIKTGGCEEDCGYCSQSSHHDTGLKAEKLMDVDTVLDAARAAKANGASRFCMGAAWR
HHHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
NPKERHMPALTEMVRGVKELGLETCMTLGMLEDEQAQQLADAGLDYYNHNLDTSPEFYGQ
CCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHCCCCCCCHHHHHH
VISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPESVPINN
HHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
LVAIEGTPLEGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDAMQAMCFLAGANS
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
MFYGDQLLTTSNPQTQRDRALFERLGIRASQADALSDNA
CEECCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA