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The map label for this gene is gcvA [H]

Identifier: 116690908

GI number: 116690908

Start: 3193869

End: 3194858

Strand: Direct

Name: gcvA [H]

Synonym: Bcen2424_2890

Alternate gene names: 116690908

Gene position: 3193869-3194858 (Clockwise)

Preceding gene: 116690901

Following gene: 116690912

Centisome position: 91.68

GC content: 68.99

Gene sequence:

>990_bases
ATGGACCTCCGCCAGCTCCCGGCGCTGAACGCGATTCGCGCGTTCGAAGCCGCCGCCCGCCACGAGAACTTCTCGCGCGC
CGCCGACGAGCTGTTCGTCACGCACGGCGCGGTCAGCCATCAGGTGCGCGCGCTCGAGGAAGAGCTCGGCGTGCAGCTGT
TCACGCGCAACGGCAAGCGGCTGTGCCTGACCGACGCCGGCATGCGCTACGCGCAGCAGATCCGCACCGCGCTGATGGTG
ATCGCCGACGCGACGCGCGACGTGCGCGCGAGCGACCGCGACCGGCGGCTGGTGGTGTCGATGCTGTCGTCGTTCGCCGC
GCGCTTCATCACGCCGCGCATCGGCACGTTCATCGAGCGGCATCCGGAAATCGACGTCGAGCTGCAGTCGACCAATTCGC
TGACCGATTTCGCGCGCGACGACGTCGATCTCGCGATCCGCTTCGGCTTCGGCAGCTATCCGGGGCTGCACGTGGAGCCG
CTGTTCGAGGAGGTGTTCTTTCCGGCGTGCGCGCCAACGCTGAACGGCGGCAAGCTGCCGCAGACGCCCGCCGATCTCGT
GCACTACCCGCTGCTGCGCTCGGACGACGAGCTGTGGCGGCCGTGGTTCGACGCGGCCGGGCTCGATACGCTGACCGAGC
CGAAACGCGGGATTCTGTACCAGGATTCGTCGAACCTGCTGCTGGCCGCGATCGAGGGCCAGGGCATCGCGCTGGTGCGC
CGCTCGCTCGCGGTGCACGACCTGCTCGACGGACGCATCGTGCGCCTGTTCGACATCGACGGGCCGAGCCCGTGGCACTA
TTTTTTCGTGTGCCCGCCGCCGCTGCTGAACACGCCGCGCGTGCAGGCGTTCCGGACCTGGCTGTTCGAGGAAGTGGCCG
AATACAAGCGGCTGTGCGACGAGCTGGACGCGCGGCGCGCGGCGGGGAAATGTCCCGCCGAGTGCGCGCTGGAGGGAGGA
TGGAAAGGGATCAGCGGCCGTTTGCGCTGA

Upstream 100 bases:

>100_bases
TCGACTCCGCCAAAAAGTGCTTCGATGGCTACAATCGTAGACACTCAAAGGCGTCCGACCAAACGACCAATTTTCACGTC
GATGTGAGGAAAATTAGCAA

Downstream 100 bases:

>100_bases
TCCCGGACGCCGGAACGAGCGCTTAAAGGACGACCTTGACCATCGGATGGCCGGTCAGCGCACGGTAGATGTTGTCGAGC
TGTTCCTGGCTCGTCGCGCG

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MDLRQLPALNAIRAFEAAARHENFSRAADELFVTHGAVSHQVRALEEELGVQLFTRNGKRLCLTDAGMRYAQQIRTALMV
IADATRDVRASDRDRRLVVSMLSSFAARFITPRIGTFIERHPEIDVELQSTNSLTDFARDDVDLAIRFGFGSYPGLHVEP
LFEEVFFPACAPTLNGGKLPQTPADLVHYPLLRSDDELWRPWFDAAGLDTLTEPKRGILYQDSSNLLLAAIEGQGIALVR
RSLAVHDLLDGRIVRLFDIDGPSPWHYFFVCPPPLLNTPRVQAFRTWLFEEVAEYKRLCDELDARRAAGKCPAECALEGG
WKGISGRLR

Sequences:

>Translated_329_residues
MDLRQLPALNAIRAFEAAARHENFSRAADELFVTHGAVSHQVRALEEELGVQLFTRNGKRLCLTDAGMRYAQQIRTALMV
IADATRDVRASDRDRRLVVSMLSSFAARFITPRIGTFIERHPEIDVELQSTNSLTDFARDDVDLAIRFGFGSYPGLHVEP
LFEEVFFPACAPTLNGGKLPQTPADLVHYPLLRSDDELWRPWFDAAGLDTLTEPKRGILYQDSSNLLLAAIEGQGIALVR
RSLAVHDLLDGRIVRLFDIDGPSPWHYFFVCPPPLLNTPRVQAFRTWLFEEVAEYKRLCDELDARRAAGKCPAECALEGG
WKGISGRLR
>Mature_329_residues
MDLRQLPALNAIRAFEAAARHENFSRAADELFVTHGAVSHQVRALEEELGVQLFTRNGKRLCLTDAGMRYAQQIRTALMV
IADATRDVRASDRDRRLVVSMLSSFAARFITPRIGTFIERHPEIDVELQSTNSLTDFARDDVDLAIRFGFGSYPGLHVEP
LFEEVFFPACAPTLNGGKLPQTPADLVHYPLLRSDDELWRPWFDAAGLDTLTEPKRGILYQDSSNLLLAAIEGQGIALVR
RSLAVHDLLDGRIVRLFDIDGPSPWHYFFVCPPPLLNTPRVQAFRTWLFEEVAEYKRLCDELDARRAAGKCPAECALEGG
WKGISGRLR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=38.6986301369863, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1786448, Length=298, Percent_Identity=31.8791946308725, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=29.0102389078498, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=29.5918367346939, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=27.6094276094276, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI157672245, Length=158, Percent_Identity=37.3417721518987, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787128, Length=139, Percent_Identity=29.4964028776978, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693105, Length=167, Percent_Identity=26.9461077844311, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081978, Length=157, Percent_Identity=29.2993630573248, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787589, Length=140, Percent_Identity=31.4285714285714, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788748, Length=168, Percent_Identity=27.3809523809524, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790262, Length=218, Percent_Identity=25.6880733944954, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36975; Mature: 36975

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRQLPALNAIRAFEAAARHENFSRAADELFVTHGAVSHQVRALEEELGVQLFTRNGKR
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEEECCCCE
LCLTDAGMRYAQQIRTALMVIADATRDVRASDRDRRLVVSMLSSFAARFITPRIGTFIER
EEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHH
HPEIDVELQSTNSLTDFARDDVDLAIRFGFGSYPGLHVEPLFEEVFFPACAPTLNGGKLP
CCCCEEEECCCCCHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
QTPADLVHYPLLRSDDELWRPWFDAAGLDTLTEPKRGILYQDSSNLLLAAIEGQGIALVR
CCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCHHHHH
RSLAVHDLLDGRIVRLFDIDGPSPWHYFFVCPPPLLNTPRVQAFRTWLFEEVAEYKRLCD
HHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELDARRAAGKCPAECALEGGWKGISGRLR
HHHHHHHCCCCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDLRQLPALNAIRAFEAAARHENFSRAADELFVTHGAVSHQVRALEEELGVQLFTRNGKR
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEEECCCCE
LCLTDAGMRYAQQIRTALMVIADATRDVRASDRDRRLVVSMLSSFAARFITPRIGTFIER
EEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHH
HPEIDVELQSTNSLTDFARDDVDLAIRFGFGSYPGLHVEPLFEEVFFPACAPTLNGGKLP
CCCCEEEECCCCCHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
QTPADLVHYPLLRSDDELWRPWFDAAGLDTLTEPKRGILYQDSSNLLLAAIEGQGIALVR
CCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCHHHHH
RSLAVHDLLDGRIVRLFDIDGPSPWHYFFVCPPPLLNTPRVQAFRTWLFEEVAEYKRLCD
HHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELDARRAAGKCPAECALEGGWKGISGRLR
HHHHHHHCCCCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]