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The map label for this gene is lipB [H]

Identifier: 116690906

GI number: 116690906

Start: 3192600

End: 3193355

Strand: Reverse

Name: lipB [H]

Synonym: Bcen2424_2888

Alternate gene names: 116690906

Gene position: 3193355-3192600 (Counterclockwise)

Preceding gene: 116690907

Following gene: 116690905

Centisome position: 91.66

GC content: 67.99

Gene sequence:

>756_bases
ATGTCCGTTTCGCCGGTTTCCATCGTGTCCACGCCTGTCGCCGTTTCCGCATCGCCCGCCGGAGCCCCGGATCAGCCGGT
CCAGCCGGTCACCGTGCGGTGGCGGGGTCGCGAGGCATACGAGGCGAGCTTCGACGCGATGCGCGCGTTCACCGACACGC
GCACGGCCGACACCGGCGACGAGATCTGGGTGGTCGAGCATCCGCCCGTCTACACGCTCGGCCAGGCCGGCGACCCGGCG
CACCTGCTGGTGGCCGACAGCGGCGTGCCGCTCGTGAAGGTCGACCGCGGCGGACAAATCACCTACCACGGCCCCGGCCA
GATCGTCGTCTACCTGCTGCTGGACCTGCGCCGGCGCAAGCTGATGGTGCGCACGCTCGTGACGAAGATCGAGGAGGCCG
TGATCGAAACCCTCGCGGCGTATAATCTCGCTTCGGTCCGCAAGGCGGGCGCGCCCGGGATCTACGTGGCGTCCGGCGTG
CACGAGGGCGCGAAGATCGCGGCCCTCGGCCTGAAGATCCGCAACGGCTGCAGCTATCACGGGCTGAGCCTGAATGTGAA
GATGGATCTGCGCCCGTTTCTTGCGATCAACCCGTGCGGCTATGCCGGACTGGAAACCGTCGACATGGCGAGCCTCGAGG
TTGCCGCCGACTGGAACGACGTCGCCCACACGCTGGTGGGCCGTCTGATCGCCAACCTCGACGGCGCATCCGCGGCCGCC
GACAAGCCGCACGCACTGGAACAATCGAATGACTGA

Upstream 100 bases:

>100_bases
CTGGCGCACGGTCTGACCATTCTTTCATCCGACGGATACCGGCGATCCGTCCCCCTCCCCAGGCCGGCGAGACCCACGGG
TTTTCGGTAAACTACCGCCC

Downstream 100 bases:

>100_bases
CGTTACCGCTTCTCCCGCACCGGCCGATACGGCCGCGACCCCTGCCTACGATCCGACCGCCAAGCAGAAGGCGCAGGCGA
AGACGGCGCGCATCCCGATC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSVSPVSIVSTPVAVSASPAGAPDQPVQPVTVRWRGREAYEASFDAMRAFTDTRTADTGDEIWVVEHPPVYTLGQAGDPA
HLLVADSGVPLVKVDRGGQITYHGPGQIVVYLLLDLRRRKLMVRTLVTKIEEAVIETLAAYNLASVRKAGAPGIYVASGV
HEGAKIAALGLKIRNGCSYHGLSLNVKMDLRPFLAINPCGYAGLETVDMASLEVAADWNDVAHTLVGRLIANLDGASAAA
DKPHALEQSND

Sequences:

>Translated_251_residues
MSVSPVSIVSTPVAVSASPAGAPDQPVQPVTVRWRGREAYEASFDAMRAFTDTRTADTGDEIWVVEHPPVYTLGQAGDPA
HLLVADSGVPLVKVDRGGQITYHGPGQIVVYLLLDLRRRKLMVRTLVTKIEEAVIETLAAYNLASVRKAGAPGIYVASGV
HEGAKIAALGLKIRNGCSYHGLSLNVKMDLRPFLAINPCGYAGLETVDMASLEVAADWNDVAHTLVGRLIANLDGASAAA
DKPHALEQSND
>Mature_250_residues
SVSPVSIVSTPVAVSASPAGAPDQPVQPVTVRWRGREAYEASFDAMRAFTDTRTADTGDEIWVVEHPPVYTLGQAGDPAH
LLVADSGVPLVKVDRGGQITYHGPGQIVVYLLLDLRRRKLMVRTLVTKIEEAVIETLAAYNLASVRKAGAPGIYVASGVH
EGAKIAALGLKIRNGCSYHGLSLNVKMDLRPFLAINPCGYAGLETVDMASLEVAADWNDVAHTLVGRLIANLDGASAAAD
KPHALEQSND

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI87081767, Length=206, Percent_Identity=50.4854368932039, Blast_Score=209, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323268, Length=172, Percent_Identity=29.0697674418605, Blast_Score=82, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21355483, Length=191, Percent_Identity=32.4607329842932, Blast_Score=81, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 26616; Mature: 26484

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSPVSIVSTPVAVSASPAGAPDQPVQPVTVRWRGREAYEASFDAMRAFTDTRTADTGD
CCCCCCEEEECCEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCC
EIWVVEHPPVYTLGQAGDPAHLLVADSGVPLVKVDRGGQITYHGPGQIVVYLLLDLRRRK
EEEEEECCCEEEECCCCCCEEEEEECCCCCEEEECCCCEEEEECCCCEEHHHHHHHHHHH
LMVRTLVTKIEEAVIETLAAYNLASVRKAGAPGIYVASGVHEGAKIAALGLKIRNGCSYH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEEECCCCCC
GLSLNVKMDLRPFLAINPCGYAGLETVDMASLEVAADWNDVAHTLVGRLIANLDGASAAA
CEEEEEEECCCCEEEECCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCC
DKPHALEQSND
CCCCCCCCCCC
>Mature Secondary Structure 
SVSPVSIVSTPVAVSASPAGAPDQPVQPVTVRWRGREAYEASFDAMRAFTDTRTADTGD
CCCCCEEEECCEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCC
EIWVVEHPPVYTLGQAGDPAHLLVADSGVPLVKVDRGGQITYHGPGQIVVYLLLDLRRRK
EEEEEECCCEEEECCCCCCEEEEEECCCCCEEEECCCCEEEEECCCCEEHHHHHHHHHHH
LMVRTLVTKIEEAVIETLAAYNLASVRKAGAPGIYVASGVHEGAKIAALGLKIRNGCSYH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEEECCCCCC
GLSLNVKMDLRPFLAINPCGYAGLETVDMASLEVAADWNDVAHTLVGRLIANLDGASAAA
CEEEEEEECCCCEEEECCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCC
DKPHALEQSND
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA