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The map label for this gene is lipA [H]

Identifier: 116690905

GI number: 116690905

Start: 3191615

End: 3192607

Strand: Reverse

Name: lipA [H]

Synonym: Bcen2424_2887

Alternate gene names: 116690905

Gene position: 3192607-3191615 (Counterclockwise)

Preceding gene: 116690906

Following gene: 116690904

Centisome position: 91.64

GC content: 65.66

Gene sequence:

>993_bases
ATGACTGACGTTACCGCTTCTCCCGCACCGGCCGATACGGCCGCGACCCCTGCCTACGATCCGACCGCCAAGCAGAAGGC
GCAGGCGAAGACGGCGCGCATCCCGATCAAGGTCATTCCGATCGAAAAGCTGAAGAAGCCCGAGTGGATCCGCGTGAAGG
CGGCCACCGGCAGCTCGCGTTTCAACGAGATCAAGACGATCCTGCGCGAGCACAACCTGCACACCGTGTGCGAGGAAGCG
AGCTGCCCGAACATCGGCGAATGCTTCGGCAAGGGCACCGCGACGTTCATGATCATGGGCGACAAGTGCACGCGCCGCTG
CCCGTTCTGCGACGTCGGCCACGGCCGGCCCGATCCGCTCGACGCGGATGAACCGAAGAACCTCGCGCGCACGATCGCGG
CGCTCAAGCTCAAGTACGTGGTGATCACGAGCGTCGACCGCGACGACCTGCGCGACGGCGGCGCTGCCCACTTCGTCGAG
TGCATTCGCGAAACGCGTGCGCAGTCGCCGGACACGCGCATCGAGATCCTGACGCCGGACTTCCGCGGCCGTCTCGACCG
CGCGATCTCGATCCTGAACGCCGCGCCGCCCGACGTGATGAACCACAACCTCGAAACGGTGCCGCGCCTGTACAAGGAAG
CGCGCCCGGGTTCGGACTACGCGCACTCGCTGAAGCTGCTGAAGGATTTCAAGGCGCTGCATCCGGACGTCGCGACGAAG
TCGGGCCTGATGGTCGGCCTCGGCGAGACGGAAGAGGAAATCCTGCAGGTGATGCGCGACCTGCGCGCGCACGACGTCGA
CATGCTGACGATCGGCCAGTACCTGCAGCCGTCCGAGCACCACCTGCCGGTGCGTGCGTACGTGCATCCGGACACGTTCA
AGATGTACGAGGAAGAAGCGTACAAGATGGGCTTCACGCACGCGGCCGTCGGCGCGATGGTGCGCTCGAGCTATCACGCC
GATCTGCAGGCGCATGGGGCCGGGGTGGTCTGA

Upstream 100 bases:

>100_bases
GACTGGAACGACGTCGCCCACACGCTGGTGGGCCGTCTGATCGCCAACCTCGACGGCGCATCCGCGGCCGCCGACAAGCC
GCACGCACTGGAACAATCGA

Downstream 100 bases:

>100_bases
GCGTCGCGCTCGCTTCCATCTGCAACAGTCTCGAGCAGTCGAAAAAAGCCCGCAGCGATGCGGGCTTTTTCGTTGGCGCG
GCAGTTATCCGTGCATGCGC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTDVTASPAPADTAATPAYDPTAKQKAQAKTARIPIKVIPIEKLKKPEWIRVKAATGSSRFNEIKTILREHNLHTVCEEA
SCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDADEPKNLARTIAALKLKYVVITSVDRDDLRDGGAAHFVE
CIRETRAQSPDTRIEILTPDFRGRLDRAISILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPDVATK
SGLMVGLGETEEEILQVMRDLRAHDVDMLTIGQYLQPSEHHLPVRAYVHPDTFKMYEEEAYKMGFTHAAVGAMVRSSYHA
DLQAHGAGVV

Sequences:

>Translated_330_residues
MTDVTASPAPADTAATPAYDPTAKQKAQAKTARIPIKVIPIEKLKKPEWIRVKAATGSSRFNEIKTILREHNLHTVCEEA
SCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDADEPKNLARTIAALKLKYVVITSVDRDDLRDGGAAHFVE
CIRETRAQSPDTRIEILTPDFRGRLDRAISILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPDVATK
SGLMVGLGETEEEILQVMRDLRAHDVDMLTIGQYLQPSEHHLPVRAYVHPDTFKMYEEEAYKMGFTHAAVGAMVRSSYHA
DLQAHGAGVV
>Mature_329_residues
TDVTASPAPADTAATPAYDPTAKQKAQAKTARIPIKVIPIEKLKKPEWIRVKAATGSSRFNEIKTILREHNLHTVCEEAS
CPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDADEPKNLARTIAALKLKYVVITSVDRDDLRDGGAAHFVEC
IRETRAQSPDTRIEILTPDFRGRLDRAISILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPDVATKS
GLMVGLGETEEEILQVMRDLRAHDVDMLTIGQYLQPSEHHLPVRAYVHPDTFKMYEEEAYKMGFTHAAVGAMVRSSYHAD
LQAHGAGVV

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=286, Percent_Identity=48.2517482517483, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI37577164, Length=250, Percent_Identity=49.2, Blast_Score=242, Evalue=3e-64,
Organism=Escherichia coli, GI1786846, Length=303, Percent_Identity=64.3564356435644, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32564533, Length=254, Percent_Identity=46.0629921259843, Blast_Score=230, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6324770, Length=289, Percent_Identity=44.9826989619377, Blast_Score=249, Evalue=6e-67,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=47.2027972027972, Blast_Score=265, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36504; Mature: 36373

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVTASPAPADTAATPAYDPTAKQKAQAKTARIPIKVIPIEKLKKPEWIRVKAATGSSR
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECHHHCCCCCEEEEEECCCCHH
FNEIKTILREHNLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPL
HHHHHHHHHHCCHHHHHHCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCCC
DADEPKNLARTIAALKLKYVVITSVDRDDLRDGGAAHFVECIRETRAQSPDTRIEILTPD
CCCCHHHHHHHHHHHHEEEEEEEECCHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCH
FRGRLDRAISILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPDVATK
HHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
SGLMVGLGETEEEILQVMRDLRAHDVDMLTIGQYLQPSEHHLPVRAYVHPDTFKMYEEEA
CCEEEECCCCHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCCEEEEECCHHHHHHHHHH
YKMGFTHAAVGAMVRSSYHADLQAHGAGVV
HHHCCHHHHHHHHHHHHHHCCHHCCCCCCC
>Mature Secondary Structure 
TDVTASPAPADTAATPAYDPTAKQKAQAKTARIPIKVIPIEKLKKPEWIRVKAATGSSR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECHHHCCCCCEEEEEECCCCHH
FNEIKTILREHNLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPL
HHHHHHHHHHCCHHHHHHCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCCC
DADEPKNLARTIAALKLKYVVITSVDRDDLRDGGAAHFVECIRETRAQSPDTRIEILTPD
CCCCHHHHHHHHHHHHEEEEEEEECCHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCH
FRGRLDRAISILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPDVATK
HHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
SGLMVGLGETEEEILQVMRDLRAHDVDMLTIGQYLQPSEHHLPVRAYVHPDTFKMYEEEA
CCEEEECCCCHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCCEEEEECCHHHHHHHHHH
YKMGFTHAAVGAMVRSSYHADLQAHGAGVV
HHHCCHHHHHHHHHHHHHHCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA