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The map label for this gene is glnB [H]

Identifier: 116690886

GI number: 116690886

Start: 3165636

End: 3166046

Strand: Reverse

Name: glnB [H]

Synonym: Bcen2424_2866

Alternate gene names: 116690886

Gene position: 3166046-3165636 (Counterclockwise)

Preceding gene: 116690889

Following gene: 116690885

Centisome position: 90.88

GC content: 61.56

Gene sequence:

>411_bases
ATGCGAGTTGCTGAGAAGAGTCCGTTACAACATTCCAACTCATTCGACGGGACAACCCAGCGAGAGGACTTCATGAAACT
CATTACCGCAATCATCAAGCCGTTCAAGCTCGATGAGACGCGCGAGGCGCTGTCGGCGCTTGGCGTCTCCGGCATCACGG
TGACGGAGGTGAAAGGGTTCGGGCGCCAGAAGGGGCACACCGAGCTGTACCGCGGCGCCGAATACGTGGTCGATTTCCTG
CCGAAGATGAAGATCGAGGCGGCCGTGTCCGACGATCTCGTCGACCAGGCGGTGGAAGCGATCGAGCGGGCCGCGCGCAC
CGGCAAGATCGGCGACGGCAAGATCTTCGTCACGCCGATCGAACAGGTGATCCGGATTCGCACCGGGGAGACAGGCGCGG
ACGCGCTGTAA

Upstream 100 bases:

>100_bases
ACCGGGCCATGTTGGCCATTCGGCCGGGCTTTTGCGCCTTACATCGCGGGATGCGACCCCGTTCCGGAGCCGTTCGAGAC
GGTGTTCGGGCCAACATGGC

Downstream 100 bases:

>100_bases
CAGAACAGACAAGACAAGCGACAAGAGGAAACCCAAGATGCGAAAACTTCTGATGTCCCTGCTGATGGCCGGTTCGCTGA
TCGCGACCGGCGTCGGCTCC

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MRVAEKSPLQHSNSFDGTTQREDFMKLITAIIKPFKLDETREALSALGVSGITVTEVKGFGRQKGHTELYRGAEYVVDFL
PKMKIEAAVSDDLVDQAVEAIERAARTGKIGDGKIFVTPIEQVIRIRTGETGADAL

Sequences:

>Translated_136_residues
MRVAEKSPLQHSNSFDGTTQREDFMKLITAIIKPFKLDETREALSALGVSGITVTEVKGFGRQKGHTELYRGAEYVVDFL
PKMKIEAAVSDDLVDQAVEAIERAARTGKIGDGKIFVTPIEQVIRIRTGETGADAL
>Mature_136_residues
MRVAEKSPLQHSNSFDGTTQREDFMKLITAIIKPFKLDETREALSALGVSGITVTEVKGFGRQKGHTELYRGAEYVVDFL
PKMKIEAAVSDDLVDQAVEAIERAARTGKIGDGKIFVTPIEQVIRIRTGETGADAL

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=66.0714285714286, Blast_Score=160, Evalue=3e-41,
Organism=Escherichia coli, GI1788904, Length=106, Percent_Identity=73.5849056603774, Blast_Score=159, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 14908; Mature: 14908

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAEKSPLQHSNSFDGTTQREDFMKLITAIIKPFKLDETREALSALGVSGITVTEVKGF
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHCCC
GRQKGHTELYRGAEYVVDFLPKMKIEAAVSDDLVDQAVEAIERAARTGKIGDGKIFVTPI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECH
EQVIRIRTGETGADAL
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRVAEKSPLQHSNSFDGTTQREDFMKLITAIIKPFKLDETREALSALGVSGITVTEVKGF
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHCCC
GRQKGHTELYRGAEYVVDFLPKMKIEAAVSDDLVDQAVEAIERAARTGKIGDGKIFVTPI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECH
EQVIRIRTGETGADAL
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1702507; 2878685 [H]