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The map label for this gene is leuA [H]

Identifier: 116690832

GI number: 116690832

Start: 3107830

End: 3109476

Strand: Direct

Name: leuA [H]

Synonym: Bcen2424_2812

Alternate gene names: 116690832

Gene position: 3107830-3109476 (Clockwise)

Preceding gene: 116690831

Following gene: 116690835

Centisome position: 89.21

GC content: 68.18

Gene sequence:

>1647_bases
ATGATGCGCAATCCGCAAGACAAGTACCGTCCGTTCGAGCCCGTCCGCCTCAATGGCCGCCGCTGGCCGGCCCGCACCAT
CGAGCGTGCACCCGTGTGGATGAGCACCGACCTGCGCGACGGCAACCAGTCGCTGATCGAGCCGATGAGCATCGAGCAGA
AGCTCGAGTTCTTCGAAATGCTGGTCGCCATCGGGTTCAAGGAGATCGAAGTCGGTTTTCCGTCGGCGTCGCAAACCGAC
TTCGATTTCGTGCGCAAGCTGATCGACGACAAGCGGATTCCCGACGACGTGACGATCGAGGTGCTCGTGCAGTCTCGCGA
AGACCTGATCGCTCGTACGTTCGATGCGCTCGAAGGCGTGCCGCGCGCGATCGTGCACCTGTACAACGCGGTCTGCCCGT
CGTTCCGCCGCATCGTGTTCGGGATGTCGAAGAACGACGTAAAGGCGCTCGCGATCGACGGCACGCGCATCATCAAGGCA
CATGCGGCCGCGCGCCCCTATACCCGCTGGACCTTCCAGTACTCGCCGGAAACCTTCAGCATGACCGAGCTGACGTTCGC
GCGCGAGATCTGCGACGCGGTCGCGCAGACGTGGCGCCCGACCCGCGACCACAAGATGATCGTCAACCTGCCGGCCACCG
TCGAGGCCGCGACCCCGAACGTGTTCGCCGACCAGATCGAATGGATGGACCGCAACCTCGCGTATCGCGACAGCATCGTG
CTGTCCGTGCATCCGCACAACGATCGCGGCACCGCGGTCGCGGCGGCCGAACTCGCGCTGCTCGCGGGCGCCGACCGCAT
CGAGGGCTGCCTGTTCGGCAACGGCGAGCGCACCGGCAACGTCGATCTCGTCACGCTCGCGTTGAACCTCTACACGCAAG
GCATCGACCCGGGCCTCGATTTCTCCGACATCGACGCGGTGCGCCGCGTCGTGGAGCACTGCAACCAGATTCCCGTGCAT
CCGCGCCACCCGTACGCGGGCGATCTCGTGTTCACCGCGTTCTCGGGCTCGCACCAGGATGCGATCCGCAAGGGGTTCGC
GCAGCAGCGTCCCGATGCGATCTGGGAGGTCCCGTACCTGCCGATCGACCCGGCCGACCTCGGCCGCAGCTATGACGCGG
TGATCCGCGTGAACAGCCAGTCCGGCAAGGGCGGCGCGACGTTCCTGCTCGAGCGCGGGATGGGCTTCACGCCGACGCGC
CGCGTCCAGATCGAGTTCAGCCATGCGGTGCAGACGCTCGCCGACGCGTCGGGCGAGGAAGTGACGGGCGACGCGATCTG
CGCGCTGTTCTCCCGCGAATTCTTCGAGACCGACGGCCCGGCCGCACGCCACGGCAGCGGCGCGCGCTGGCAAAACCGCG
AGATCGCGGTGGCGCCCGCCGCCGACGCGACGCCCGACGACGCCGTGCGACGTTTCGCCGCGGCATTTGCGACGGCCGCT
GGCGCCGCGATCGACGTCGCGTCGTGCGAATACACGCGCACCGCGGACGGCCGGATCGCGGTATCGGTCGGCTGCCGGGT
CGGCGACGCGCCGCTGCGGCACGGGGTCGGCGTGCACGCCGATGCGGCGAGCGCGGCACTCGATGCGGTCGTCAGCGCGA
TCAACCGCTCGGCATGGCACTGCGCCGATCGACGCGCGGCGGCCTGA

Upstream 100 bases:

>100_bases
TCCGCATCCGCGGCGCCATCGCGCGCCGGGTGCGAGGCAGCGCCAACCGTCGCCCATGCCGCCCGTCTTCGCTCGCGACT
TGAGACCCGAGGTCCAGAAG

Downstream 100 bases:

>100_bases
GCGCCGGGCTTCGATGCAGGGTTGAAAGGTCAGCGAGCGGTGCGTGACCGCCCGTGCGCCGCGCGATCCGCGCCGCACGG
GGCAGCAAAAAGCGGCTCAG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MMRNPQDKYRPFEPVRLNGRRWPARTIERAPVWMSTDLRDGNQSLIEPMSIEQKLEFFEMLVAIGFKEIEVGFPSASQTD
FDFVRKLIDDKRIPDDVTIEVLVQSREDLIARTFDALEGVPRAIVHLYNAVCPSFRRIVFGMSKNDVKALAIDGTRIIKA
HAAARPYTRWTFQYSPETFSMTELTFAREICDAVAQTWRPTRDHKMIVNLPATVEAATPNVFADQIEWMDRNLAYRDSIV
LSVHPHNDRGTAVAAAELALLAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLDFSDIDAVRRVVEHCNQIPVH
PRHPYAGDLVFTAFSGSHQDAIRKGFAQQRPDAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGATFLLERGMGFTPTR
RVQIEFSHAVQTLADASGEEVTGDAICALFSREFFETDGPAARHGSGARWQNREIAVAPAADATPDDAVRRFAAAFATAA
GAAIDVASCEYTRTADGRIAVSVGCRVGDAPLRHGVGVHADAASAALDAVVSAINRSAWHCADRRAAA

Sequences:

>Translated_548_residues
MMRNPQDKYRPFEPVRLNGRRWPARTIERAPVWMSTDLRDGNQSLIEPMSIEQKLEFFEMLVAIGFKEIEVGFPSASQTD
FDFVRKLIDDKRIPDDVTIEVLVQSREDLIARTFDALEGVPRAIVHLYNAVCPSFRRIVFGMSKNDVKALAIDGTRIIKA
HAAARPYTRWTFQYSPETFSMTELTFAREICDAVAQTWRPTRDHKMIVNLPATVEAATPNVFADQIEWMDRNLAYRDSIV
LSVHPHNDRGTAVAAAELALLAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLDFSDIDAVRRVVEHCNQIPVH
PRHPYAGDLVFTAFSGSHQDAIRKGFAQQRPDAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGATFLLERGMGFTPTR
RVQIEFSHAVQTLADASGEEVTGDAICALFSREFFETDGPAARHGSGARWQNREIAVAPAADATPDDAVRRFAAAFATAA
GAAIDVASCEYTRTADGRIAVSVGCRVGDAPLRHGVGVHADAASAALDAVVSAINRSAWHCADRRAAA
>Mature_548_residues
MMRNPQDKYRPFEPVRLNGRRWPARTIERAPVWMSTDLRDGNQSLIEPMSIEQKLEFFEMLVAIGFKEIEVGFPSASQTD
FDFVRKLIDDKRIPDDVTIEVLVQSREDLIARTFDALEGVPRAIVHLYNAVCPSFRRIVFGMSKNDVKALAIDGTRIIKA
HAAARPYTRWTFQYSPETFSMTELTFAREICDAVAQTWRPTRDHKMIVNLPATVEAATPNVFADQIEWMDRNLAYRDSIV
LSVHPHNDRGTAVAAAELALLAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLDFSDIDAVRRVVEHCNQIPVH
PRHPYAGDLVFTAFSGSHQDAIRKGFAQQRPDAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGATFLLERGMGFTPTR
RVQIEFSHAVQTLADASGEEVTGDAICALFSREFFETDGPAARHGSGARWQNREIAVAPAADATPDDAVRRFAAAFATAA
GAAIDVASCEYTRTADGRIAVSVGCRVGDAPLRHGVGVHADAASAALDAVVSAINRSAWHCADRRAAA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=533, Percent_Identity=24.577861163227, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6324225, Length=575, Percent_Identity=47.8260869565217, Blast_Score=524, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6324682, Length=442, Percent_Identity=54.2986425339367, Blast_Score=514, Evalue=1e-146,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 60219; Mature: 60219

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRNPQDKYRPFEPVRLNGRRWPARTIERAPVWMSTDLRDGNQSLIEPMSIEQKLEFFEM
CCCCCCHHCCCCCCEEECCCCCCHHHHCCCCEEEECCCCCCCHHHHCCHHHHHHHHHHHH
LVAIGFKEIEVGFPSASQTDFDFVRKLIDDKRIPDDVTIEVLVQSREDLIARTFDALEGV
HHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCH
PRAIVHLYNAVCPSFRRIVFGMSKNDVKALAIDGTRIIKAHAAARPYTRWTFQYSPETFS
HHHHHHHHHHHCHHHHHHHHCCCCCCEEEEEECCCEEEEHHHCCCCCEEEEEEECCCCCC
MTELTFAREICDAVAQTWRPTRDHKMIVNLPATVEAATPNVFADQIEWMDRNLAYRDSIV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHCCCEEECEEE
LSVHPHNDRGTAVAAAELALLAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLD
EEEECCCCCCCHHHHHHHHHHHCCHHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCCCC
FSDIDAVRRVVEHCNQIPVHPRHPYAGDLVFTAFSGSHQDAIRKGFAQQRPDAIWEVPYL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEECCCC
PIDPADLGRSYDAVIRVNSQSGKGGATFLLERGMGFTPTRRVQIEFSHAVQTLADASGEE
CCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHCCCCCC
VTGDAICALFSREFFETDGPAARHGSGARWQNREIAVAPAADATPDDAVRRFAAAFATAA
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHC
GAAIDVASCEYTRTADGRIAVSVGCRVGDAPLRHGVGVHADAASAALDAVVSAINRSAWH
CCEEEECCCCEEECCCCEEEEEECCEECCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
CADRRAAA
HCHHCCCC
>Mature Secondary Structure
MMRNPQDKYRPFEPVRLNGRRWPARTIERAPVWMSTDLRDGNQSLIEPMSIEQKLEFFEM
CCCCCCHHCCCCCCEEECCCCCCHHHHCCCCEEEECCCCCCCHHHHCCHHHHHHHHHHHH
LVAIGFKEIEVGFPSASQTDFDFVRKLIDDKRIPDDVTIEVLVQSREDLIARTFDALEGV
HHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCH
PRAIVHLYNAVCPSFRRIVFGMSKNDVKALAIDGTRIIKAHAAARPYTRWTFQYSPETFS
HHHHHHHHHHHCHHHHHHHHCCCCCCEEEEEECCCEEEEHHHCCCCCEEEEEEECCCCCC
MTELTFAREICDAVAQTWRPTRDHKMIVNLPATVEAATPNVFADQIEWMDRNLAYRDSIV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHCCCEEECEEE
LSVHPHNDRGTAVAAAELALLAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLD
EEEECCCCCCCHHHHHHHHHHHCCHHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCCCC
FSDIDAVRRVVEHCNQIPVHPRHPYAGDLVFTAFSGSHQDAIRKGFAQQRPDAIWEVPYL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEECCCC
PIDPADLGRSYDAVIRVNSQSGKGGATFLLERGMGFTPTRRVQIEFSHAVQTLADASGEE
CCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHCCCCCC
VTGDAICALFSREFFETDGPAARHGSGARWQNREIAVAPAADATPDDAVRRFAAAFATAA
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHC
GAAIDVASCEYTRTADGRIAVSVGCRVGDAPLRHGVGVHADAASAALDAVVSAINRSAWH
CCEEEECCCCEEECCCCEEEEEECCEECCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
CADRRAAA
HCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA