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The map label for this gene is rpoH [H]

Identifier: 116690835

GI number: 116690835

Start: 3110979

End: 3111914

Strand: Direct

Name: rpoH [H]

Synonym: Bcen2424_2815

Alternate gene names: 116690835

Gene position: 3110979-3111914 (Clockwise)

Preceding gene: 116690832

Following gene: 116690836

Centisome position: 89.3

GC content: 66.35

Gene sequence:

>936_bases
GTGAGCCACGCCCTGACCCTCCCGAACACGCTGAGCCCGACGCCGGCCAAGGCCGAATCGGCAGGCTCGCTGGCGCTCGC
AGCCCAATCGATGTTGCCCGGCCAGCTCGGCAACATCGACGCGTACATCCAGGCCGTCAATCGCATTCCGCTGCTGACCG
CCGAAGAAGAACGCCGCTACGCAACCGAATTCCGCGAGGACAACAACCTCGACGCGGCGCGCCGCCTCGTGCTGTCGCAC
CTGCGTCTCGTCGTGTCGATCGCGCGCAACTATCTCGGCTACGGCCTGCCGCACGGCGACCTGATCCAGGAAGGCAACAT
CGGCCTGATGAAAGCCGTGAAGCGGTTCGACCCGGCGCAAAACGTGCGCCTGGTGTCGTATGCGATTCACTGGATCAAGG
CCGAGATCCACGAGTACATCCTGCGCAACTGGCGCATGGTGAAGGTCGCGACCACGAAGGCGCAGCGCAAGCTGTTCTTC
AACCTGCGCAGCCACAAGAAGAGCATGCAGGCGATGACGCCCGAGGAAATCGACGGCCTCGCGCAGGAGCTCAACGTCAA
GCGCGAGGAAGTCACCGAGATGGAAACGCGCCTGTCGGGCGGCGACATCGCGCTCGAAGGCCAGGTGGAAGACGGCGAGG
AGTCGTACGCGCCGATCGCCTACCTGGCCGACTCGCACAACGAGCCGACCGCCGTGCTGGCCGCACGTCAGCGCGACATG
CTGCAGACGGACGGCATCGCGCAGGCGCTCGACGCGCTCGACGCGCGCAGCCGCCGCATCATCGAGGCGCGCTGGCTGCA
CGTCGACGACGACGGCTCGGGCGGCTCGACGCTGCACGATCTCGCCGCCGAGTTCGGTGTGTCGGCGGAACGCATCCGCC
AGATCGAAGCGAGCGCGATGAAGAAGATGCGCACGGCCCTCGCCGAATACGCGTAA

Upstream 100 bases:

>100_bases
CGCTCTAAGGAATTAGCACTCGTATTGCGGGAGTGCTAAGATGACCCACGGAATCTCATTCGAGACCGGGGTTTGTCCCT
GATTCCAAAGGAGTTTTTTA

Downstream 100 bases:

>100_bases
ATCCCGGCGATTTAGAGCGCTTCATCGAAAACCGCTGCCTGCCCGGCAGCGGTTTTTTTTCGTCCGTTTTTCCGCTGCTT
TTCCCCGCTTTTCAACGCGT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSHALTLPNTLSPTPAKAESAGSLALAAQSMLPGQLGNIDAYIQAVNRIPLLTAEEERRYATEFREDNNLDAARRLVLSH
LRLVVSIARNYLGYGLPHGDLIQEGNIGLMKAVKRFDPAQNVRLVSYAIHWIKAEIHEYILRNWRMVKVATTKAQRKLFF
NLRSHKKSMQAMTPEEIDGLAQELNVKREEVTEMETRLSGGDIALEGQVEDGEESYAPIAYLADSHNEPTAVLAARQRDM
LQTDGIAQALDALDARSRRIIEARWLHVDDDGSGGSTLHDLAAEFGVSAERIRQIEASAMKKMRTALAEYA

Sequences:

>Translated_311_residues
MSHALTLPNTLSPTPAKAESAGSLALAAQSMLPGQLGNIDAYIQAVNRIPLLTAEEERRYATEFREDNNLDAARRLVLSH
LRLVVSIARNYLGYGLPHGDLIQEGNIGLMKAVKRFDPAQNVRLVSYAIHWIKAEIHEYILRNWRMVKVATTKAQRKLFF
NLRSHKKSMQAMTPEEIDGLAQELNVKREEVTEMETRLSGGDIALEGQVEDGEESYAPIAYLADSHNEPTAVLAARQRDM
LQTDGIAQALDALDARSRRIIEARWLHVDDDGSGGSTLHDLAAEFGVSAERIRQIEASAMKKMRTALAEYA
>Mature_310_residues
SHALTLPNTLSPTPAKAESAGSLALAAQSMLPGQLGNIDAYIQAVNRIPLLTAEEERRYATEFREDNNLDAARRLVLSHL
RLVVSIARNYLGYGLPHGDLIQEGNIGLMKAVKRFDPAQNVRLVSYAIHWIKAEIHEYILRNWRMVKVATTKAQRKLFFN
LRSHKKSMQAMTPEEIDGLAQELNVKREEVTEMETRLSGGDIALEGQVEDGEESYAPIAYLADSHNEPTAVLAARQRDML
QTDGIAQALDALDARSRRIIEARWLHVDDDGSGGSTLHDLAAEFGVSAERIRQIEASAMKKMRTALAEYA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=281, Percent_Identity=50.5338078291815, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1789098, Length=272, Percent_Identity=32.7205882352941, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789448, Length=241, Percent_Identity=31.9502074688797, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34570; Mature: 34439

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHALTLPNTLSPTPAKAESAGSLALAAQSMLPGQLGNIDAYIQAVNRIPLLTAEEERRY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEECHHHHHH
ATEFREDNNLDAARRLVLSHLRLVVSIARNYLGYGLPHGDLIQEGNIGLMKAVKRFDPAQ
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHCCCCC
NVRLVSYAIHWIKAEIHEYILRNWRMVKVATTKAQRKLFFNLRSHKKSMQAMTPEEIDGL
CHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AQELNVKREEVTEMETRLSGGDIALEGQVEDGEESYAPIAYLADSHNEPTAVLAARQRDM
HHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
LQTDGIAQALDALDARSRRIIEARWLHVDDDGSGGSTLHDLAAEFGVSAERIRQIEASAM
HHHCHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
KKMRTALAEYA
HHHHHHHHHCC
>Mature Secondary Structure 
SHALTLPNTLSPTPAKAESAGSLALAAQSMLPGQLGNIDAYIQAVNRIPLLTAEEERRY
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEECHHHHHH
ATEFREDNNLDAARRLVLSHLRLVVSIARNYLGYGLPHGDLIQEGNIGLMKAVKRFDPAQ
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHCCCCC
NVRLVSYAIHWIKAEIHEYILRNWRMVKVATTKAQRKLFFNLRSHKKSMQAMTPEEIDGL
CHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AQELNVKREEVTEMETRLSGGDIALEGQVEDGEESYAPIAYLADSHNEPTAVLAARQRDM
HHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
LQTDGIAQALDALDARSRRIIEARWLHVDDDGSGGSTLHDLAAEFGVSAERIRQIEASAM
HHHCHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
KKMRTALAEYA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]