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The map label for this gene is maiA [H]

Identifier: 116690830

GI number: 116690830

Start: 3105654

End: 3106298

Strand: Reverse

Name: maiA [H]

Synonym: Bcen2424_2810

Alternate gene names: 116690830

Gene position: 3106298-3105654 (Counterclockwise)

Preceding gene: 116690833

Following gene: 116690828

Centisome position: 89.16

GC content: 64.34

Gene sequence:

>645_bases
ATGAAGCTCTACAGCTATTTCCGCAGTTCCGCGTCGTACCGCGTGCGGATCGCCTTGAACCTCAAGCAGCTGCCGTTCGA
CTATGCGCCCGTGCACATGCTGCGCGACGGCGGCGAGCAACTGAAGGACGCGTACCGCGCGCTGAATCCGGATGCGGTCG
TGCCGACGCTGATCGATGGCGACGCGACGCTGCAGCAGTCGCTCGCGATCATCGAATATCTCGACGAAACCCATCCCGAG
CCCCCGTTGCTGCCGAAGCAGCCGGTCGATCGTGCCTATGTGCGGGCGGTCGCGCTGCAGATCGCATGCGAAATCCACCC
GCTCAACAACCTGCGCGTGCTGAAGTACCTGAAGCACACGCTGAAGGTGCCGGAGGAAGCAAAGAACGCGTGGTACCGGC
ACTGGATCGAGGCGGGCTTCGACACGCTCGAGACGCGTCTCGCGAACGATCCGCGCACCGGCAAGCTGTGTTTCGGCGAC
ACGCCGACGCTCGCCGACGTGTGCCTTGTACCGCAGGTGTTCAACGCGAACCGCTTCTCGATCGACACGACGCGTTTCCC
CACGATCCAGCGGATCGTCGACCATGCGGCGACGATCGATGCGTTCAAGGCCGCCGAGCCGGGCGCGCAGCCGGACGCGG
AATGA

Upstream 100 bases:

>100_bases
GCGCATCGCGCCGCGCTACACTCTGGTTCCCGGCGGCACGCGCGCCGCTGCGCCGCGCCCACGCCGCCGCCGGCCGAATA
CGACCAAAGAGAGACATCCC

Downstream 100 bases:

>100_bases
GCGCGGAATGCGCGCAGCGGCCGGTGGCGGGCGGGCCGGATCGGGCGCGCATGAAAAAGGGCGCCCGAAGGCGCCCTGTG
CGAGGCGGGCCGGCGGCGTT

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKLYSYFRSSASYRVRIALNLKQLPFDYAPVHMLRDGGEQLKDAYRALNPDAVVPTLIDGDATLQQSLAIIEYLDETHPE
PPLLPKQPVDRAYVRAVALQIACEIHPLNNLRVLKYLKHTLKVPEEAKNAWYRHWIEAGFDTLETRLANDPRTGKLCFGD
TPTLADVCLVPQVFNANRFSIDTTRFPTIQRIVDHAATIDAFKAAEPGAQPDAE

Sequences:

>Translated_214_residues
MKLYSYFRSSASYRVRIALNLKQLPFDYAPVHMLRDGGEQLKDAYRALNPDAVVPTLIDGDATLQQSLAIIEYLDETHPE
PPLLPKQPVDRAYVRAVALQIACEIHPLNNLRVLKYLKHTLKVPEEAKNAWYRHWIEAGFDTLETRLANDPRTGKLCFGD
TPTLADVCLVPQVFNANRFSIDTTRFPTIQRIVDHAATIDAFKAAEPGAQPDAE
>Mature_214_residues
MKLYSYFRSSASYRVRIALNLKQLPFDYAPVHMLRDGGEQLKDAYRALNPDAVVPTLIDGDATLQQSLAIIEYLDETHPE
PPLLPKQPVDRAYVRAVALQIACEIHPLNNLRVLKYLKHTLKVPEEAKNAWYRHWIEAGFDTLETRLANDPRTGKLCFGD
TPTLADVCLVPQVFNANRFSIDTTRFPTIQRIVDHAATIDAFKAAEPGAQPDAE

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=211, Percent_Identity=48.8151658767773, Blast_Score=188, Evalue=3e-48,
Organism=Homo sapiens, GI22202622, Length=160, Percent_Identity=48.125, Blast_Score=131, Evalue=5e-31,
Organism=Homo sapiens, GI194440732, Length=211, Percent_Identity=37.914691943128, Blast_Score=128, Evalue=4e-30,
Organism=Escherichia coli, GI1789624, Length=111, Percent_Identity=39.6396396396396, Blast_Score=61, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17551302, Length=210, Percent_Identity=43.3333333333333, Blast_Score=166, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17510461, Length=211, Percent_Identity=41.2322274881517, Blast_Score=154, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17556142, Length=212, Percent_Identity=41.5094339622642, Blast_Score=143, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24645375, Length=212, Percent_Identity=48.1132075471698, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI21355859, Length=212, Percent_Identity=48.1132075471698, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI45553325, Length=212, Percent_Identity=48.1132075471698, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI21355857, Length=213, Percent_Identity=44.131455399061, Blast_Score=172, Evalue=1e-43,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24141; Mature: 24141

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYSYFRSSASYRVRIALNLKQLPFDYAPVHMLRDGGEQLKDAYRALNPDAVVPTLIDG
CCHHHHHHCCCCEEEEEEEEHHHCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCEEECC
DATLQQSLAIIEYLDETHPEPPLLPKQPVDRAYVRAVALQIACEIHPLNNLRVLKYLKHT
CHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHH
LKVPEEAKNAWYRHWIEAGFDTLETRLANDPRTGKLCFGDTPTLADVCLVPQVFNANRFS
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCEE
IDTTRFPTIQRIVDHAATIDAFKAAEPGAQPDAE
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKLYSYFRSSASYRVRIALNLKQLPFDYAPVHMLRDGGEQLKDAYRALNPDAVVPTLIDG
CCHHHHHHCCCCEEEEEEEEHHHCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCEEECC
DATLQQSLAIIEYLDETHPEPPLLPKQPVDRAYVRAVALQIACEIHPLNNLRVLKYLKHT
CHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHH
LKVPEEAKNAWYRHWIEAGFDTLETRLANDPRTGKLCFGDTPTLADVCLVPQVFNANRFS
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCEE
IDTTRFPTIQRIVDHAATIDAFKAAEPGAQPDAE
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]