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The map label for this gene is rsmD [H]

Identifier: 116690828

GI number: 116690828

Start: 3103557

End: 3104270

Strand: Reverse

Name: rsmD [H]

Synonym: Bcen2424_2808

Alternate gene names: 116690828

Gene position: 3104270-3103557 (Counterclockwise)

Preceding gene: 116690830

Following gene: 116690827

Centisome position: 89.1

GC content: 71.85

Gene sequence:

>714_bases
GTGGCCCTCGCGTCAGTGCGCGTCGCCGCTGTGGTAACGTGCGCCTGTTTGGCGGCGCGCCTCTGCGTGCGCCCGGTCCC
TCCGTCGTCGATATCCCGTATGTCCCGTTCCTCTTCCGGCCGCCCGGCGGCCCCTTCCAGTCGCGGCAAGCCGCACACGA
TCCGCATCATCGGCGGCGACTGGAAACGCACCCCGCTCGCGGTGCTCGATCTCGACGGCCTGCGCCCGACCCCCGATCGC
GTGCGCGAGACGCTGTTCAACTGGCTCGGCCAGGATCTCGAAGGCCGGCGCTGCCTCGACCTGTTCGCGGGCACCGGCGC
GCTCGGCTTCGAGGCCGCGTCGCGCGGCGCGGCGAGCGTCGTGATGGTCGAGCGCCATCCGCGCGCCGCGCAGCAGCTGC
GTGCGATCAAGGACAAGCTCGGCGCCCGTTCGGTCGAGGTTGCCGAAGCCGATGCGCTGCGGCTCGCGGCCGGGCTCACG
CCGGGCGCGTTCGACGTCGTGTTCCTCGATCCGCCGTTCGACGACCTCGCCGCGCTCGAGCGCGCGATCGCGCTGTCGGC
GCCGCTCGTCGCGGCGGGCGGCGCGCTGTACGTCGAAACGGGGGCGGAACTCGACCCGGCCGAGCACGACGCGCTGGCGG
GCTGGCAGGTGGTGAAGCACGGCAAGGCCGGTGCGGTTCACTATCATTTGCTGCGGCGCGAAAATGATGAATAA

Upstream 100 bases:

>100_bases
AGGGTGGGCCGCCGCCGGGAGCGGGGCGCCGGATGGGCCGTTCTGCGTTCGCGCGGGCGCGGCGCACGGCGGCCGAAACC
GTGCATTTTATCAGTGCGGC

Downstream 100 bases:

>100_bases
TGCGCGTTCCATACGAGGCGGCGCGCCGCAAAGGCGACGCATGCCCATCTCGGCAGCGGCGCGTGCATCGCACGGCTGCC
ATCCCGTTTGCGTGATGACA

Product: putative methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MALASVRVAAVVTCACLAARLCVRPVPPSSISRMSRSSSGRPAAPSSRGKPHTIRIIGGDWKRTPLAVLDLDGLRPTPDR
VRETLFNWLGQDLEGRRCLDLFAGTGALGFEAASRGAASVVMVERHPRAAQQLRAIKDKLGARSVEVAEADALRLAAGLT
PGAFDVVFLDPPFDDLAALERAIALSAPLVAAGGALYVETGAELDPAEHDALAGWQVVKHGKAGAVHYHLLRRENDE

Sequences:

>Translated_237_residues
MALASVRVAAVVTCACLAARLCVRPVPPSSISRMSRSSSGRPAAPSSRGKPHTIRIIGGDWKRTPLAVLDLDGLRPTPDR
VRETLFNWLGQDLEGRRCLDLFAGTGALGFEAASRGAASVVMVERHPRAAQQLRAIKDKLGARSVEVAEADALRLAAGLT
PGAFDVVFLDPPFDDLAALERAIALSAPLVAAGGALYVETGAELDPAEHDALAGWQVVKHGKAGAVHYHLLRRENDE
>Mature_236_residues
ALASVRVAAVVTCACLAARLCVRPVPPSSISRMSRSSSGRPAAPSSRGKPHTIRIIGGDWKRTPLAVLDLDGLRPTPDRV
RETLFNWLGQDLEGRRCLDLFAGTGALGFEAASRGAASVVMVERHPRAAQQLRAIKDKLGARSVEVAEADALRLAAGLTP
GAFDVVFLDPPFDDLAALERAIALSAPLVAAGGALYVETGAELDPAEHDALAGWQVVKHGKAGAVHYHLLRRENDE

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=184, Percent_Identity=42.3913043478261, Blast_Score=113, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 25092; Mature: 24961

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALASVRVAAVVTCACLAARLCVRPVPPSSISRMSRSSSGRPAAPSSRGKPHTIRIIGGD
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCC
WKRTPLAVLDLDGLRPTPDRVRETLFNWLGQDLEGRRCLDLFAGTGALGFEAASRGAASV
CCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHCCCCEEE
VMVERHPRAAQQLRAIKDKLGARSVEVAEADALRLAAGLTPGAFDVVFLDPPFDDLAALE
EEEECCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHH
RAIALSAPLVAAGGALYVETGAELDPAEHDALAGWQVVKHGKAGAVHYHLLRRENDE
HHHHHCCCHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHEEECCCCCC
>Mature Secondary Structure 
ALASVRVAAVVTCACLAARLCVRPVPPSSISRMSRSSSGRPAAPSSRGKPHTIRIIGGD
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCC
WKRTPLAVLDLDGLRPTPDRVRETLFNWLGQDLEGRRCLDLFAGTGALGFEAASRGAASV
CCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHCCCCEEE
VMVERHPRAAQQLRAIKDKLGARSVEVAEADALRLAAGLTPGAFDVVFLDPPFDDLAALE
EEEECCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHH
RAIALSAPLVAAGGALYVETGAELDPAEHDALAGWQVVKHGKAGAVHYHLLRRENDE
HHHHHCCCHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]