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The map label for this gene is aspS [H]

Identifier: 116690745

GI number: 116690745

Start: 3019825

End: 3021627

Strand: Reverse

Name: aspS [H]

Synonym: Bcen2424_2725

Alternate gene names: 116690745

Gene position: 3021627-3019825 (Counterclockwise)

Preceding gene: 116690746

Following gene: 116690744

Centisome position: 86.73

GC content: 65.0

Gene sequence:

>1803_bases
ATGTCGATGCGTACTGAATACTGCGGTCTCGTGACCGAACACCTGCTGGGCCAAACCGTGTCGCTGTGCGGCTGGGTGCA
GCGCCGCCGCGATCACGGCGGTGTGATCTTCATCGACCTGCGCGATCGTGAAGGCCTCGTGCAGGTGGTGTGCGACCCGG
ATCGCGCGGAGATGTTCGCGACCGCCGAAGGCGTGCGCAACGAGTTCTGCGTGCAGATCAAGGGCCTCGTGCGCAACCGT
CCGGACGGCACGGTCAACGCCGGCCTGAAGAGCGGCAGGATCGAAGTGCTGTGCCACGAACTGAACGTGCTGAACGCGTC
GGTCACGCCGCCGTTCCAGCTCGACGACGACAACCTGTCGGAAACGACGCGTCTCACGCACCGCGTGCTCGACCTGCGTC
GCCCGCAGATGCAGCACAACCTGCGCCTGCGCTACCGCGTCGCGATCGAGGCGCGCAAGTACCTCGACGAGCAGGGCTTC
ATCGACATCGAGACGCCGATGCTGACGAAGAGCACGCCGGAAGGCGCGCGCGACTACCTCGTGCCGTCGCGCGTGAACGC
CGGCCAGTTCTTCGCGCTACCGCAGTCGCCGCAGCTGTTCAAGCAGCTGCTGATGGTCGCGAACTTCGACCGTTACTACC
AGATCACCAAGTGCTTCCGCGACGAGGATCTCCGCGCGGACCGTCAGCCGGAATTCACGCAGATCGACTGCGAGACGTCG
TTCCTCGGCGAGCAGGAAATCCGTGACCTGTTCGAGGACATGATCCGTCACATCTTCAAGACGACGATCGACGTCGAGCT
CGACGCGAAATTCCCGGTGATGCCGTACTCGGAAGCGATGGCGCGTTTCGGTTCGGACAAGCCGGACCTGCGCGTGCAGC
TCGAATTCACCGAGCTGACCGACGCGATGAAGGACGTCGACTTCAAGGTGTTCAGCACGCCGGCCAACGCGAAGGACGGC
CGGGTCGCGGCGCTGCGCGTGCCGAAGGGCGGCGAGCTGTCGCGCGGCGACATCGACGGCTACACGGAATTCGTGCGGAT
CTACGGCGCGAAGGGCCTCGCGTGGATCAAGGTCAACGAGAAGGCGAAGGGCCGCGACGGCCTGCAAAGCCCGATCGTCA
AGAACCTGCACGACGCGTCGATCGCGGCGATCCTCGAGCGCACCGGCGCCGAAGACGGCGACATCATCTTCTTCGCGGCC
GACCGCGCGAAGGTCGTCAACGACAGCCTTGGCGCGCTGCGCCTGAAGATCGGCCATTCGGAATTCGGCAAGGCGAACGG
TCTGGTCCAGGCCGGCTGGAAGCCGCTGTGGGTCGTCGACTTCCCGATGTTCGAATACGACGACGAAGATGCGCGCTACG
TCGCAGCACACCATCCGTTTACGAGCCCGAAGGACGAGCACCTCGAGTATCTCGAGACCGACCCGGGCCGTTGCCTCGCG
AAGGCCTACGACATGGTGCTGAACGGCTGGGAAATCGGCGGCGGCTCGGTGCGTATCCACCGCGAGGAAGTGCAGAGCAA
GGTGTTCCGCGCGCTGAAGATCGGTGCGGAAGAAGCGCAACTGAAGTTCGGCTTCCTGCTGGACGCGCTGCAGTACGGCG
CGCCGCCGCACGGCGGTATCGCATTCGGTCTCGACCGCATCGTCACGATGATGGCCGGTGCCGATTCGATCCGCGACGTG
ATCGCGTTCCCGAAGACGCAGCGTGCGCAGGATCTGCTCACGCAGGCGCCGAGCCCGGTCGACGAGCGTCAGCTGCGCGA
GCTGCACATCCGTCTGCGTCAGCCGGAGCAGCCGAAGGCGTAA

Upstream 100 bases:

>100_bases
CCCGCCCGCCGTCCCGTCGAGCCGCCGCTGTAAGTAAAGAATCATCGCCCGGGCCGCGCGTTGCGGCGCCCGTTGATCAA
ACCGAACGAAAGCAAACATC

Downstream 100 bases:

>100_bases
CGCGCTTTCGCCCCCGGTCGTGTGTGCGGTCACGCACGCACGGCACCCTGAAAAAGGCGCAGTGATGCGCCTTTTTCATT
TTTTGCGGTACAGTCGCAGC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MSMRTEYCGLVTEHLLGQTVSLCGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFATAEGVRNEFCVQIKGLVRNR
PDGTVNAGLKSGRIEVLCHELNVLNASVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQHNLRLRYRVAIEARKYLDEQGF
IDIETPMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETS
FLGEQEIRDLFEDMIRHIFKTTIDVELDAKFPVMPYSEAMARFGSDKPDLRVQLEFTELTDAMKDVDFKVFSTPANAKDG
RVAALRVPKGGELSRGDIDGYTEFVRIYGAKGLAWIKVNEKAKGRDGLQSPIVKNLHDASIAAILERTGAEDGDIIFFAA
DRAKVVNDSLGALRLKIGHSEFGKANGLVQAGWKPLWVVDFPMFEYDDEDARYVAAHHPFTSPKDEHLEYLETDPGRCLA
KAYDMVLNGWEIGGGSVRIHREEVQSKVFRALKIGAEEAQLKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGADSIRDV
IAFPKTQRAQDLLTQAPSPVDERQLRELHIRLRQPEQPKA

Sequences:

>Translated_600_residues
MSMRTEYCGLVTEHLLGQTVSLCGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFATAEGVRNEFCVQIKGLVRNR
PDGTVNAGLKSGRIEVLCHELNVLNASVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQHNLRLRYRVAIEARKYLDEQGF
IDIETPMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETS
FLGEQEIRDLFEDMIRHIFKTTIDVELDAKFPVMPYSEAMARFGSDKPDLRVQLEFTELTDAMKDVDFKVFSTPANAKDG
RVAALRVPKGGELSRGDIDGYTEFVRIYGAKGLAWIKVNEKAKGRDGLQSPIVKNLHDASIAAILERTGAEDGDIIFFAA
DRAKVVNDSLGALRLKIGHSEFGKANGLVQAGWKPLWVVDFPMFEYDDEDARYVAAHHPFTSPKDEHLEYLETDPGRCLA
KAYDMVLNGWEIGGGSVRIHREEVQSKVFRALKIGAEEAQLKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGADSIRDV
IAFPKTQRAQDLLTQAPSPVDERQLRELHIRLRQPEQPKA
>Mature_599_residues
SMRTEYCGLVTEHLLGQTVSLCGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFATAEGVRNEFCVQIKGLVRNRP
DGTVNAGLKSGRIEVLCHELNVLNASVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQHNLRLRYRVAIEARKYLDEQGFI
DIETPMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETSF
LGEQEIRDLFEDMIRHIFKTTIDVELDAKFPVMPYSEAMARFGSDKPDLRVQLEFTELTDAMKDVDFKVFSTPANAKDGR
VAALRVPKGGELSRGDIDGYTEFVRIYGAKGLAWIKVNEKAKGRDGLQSPIVKNLHDASIAAILERTGAEDGDIIFFAAD
RAKVVNDSLGALRLKIGHSEFGKANGLVQAGWKPLWVVDFPMFEYDDEDARYVAAHHPFTSPKDEHLEYLETDPGRCLAK
AYDMVLNGWEIGGGSVRIHREEVQSKVFRALKIGAEEAQLKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGADSIRDVI
AFPKTQRAQDLLTQAPSPVDERQLRELHIRLRQPEQPKA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=612, Percent_Identity=38.562091503268, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI45439306, Length=285, Percent_Identity=28.7719298245614, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI194272210, Length=254, Percent_Identity=23.6220472440945, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI5031815, Length=254, Percent_Identity=23.6220472440945, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=56.5143824027073, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=297, Percent_Identity=25.9259259259259, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1790571, Length=247, Percent_Identity=25.5060728744939, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI32566633, Length=616, Percent_Identity=34.2532467532467, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17551876, Length=262, Percent_Identity=25.5725190839695, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71994340, Length=299, Percent_Identity=23.0769230769231, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17535927, Length=301, Percent_Identity=22.9235880398671, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=301, Percent_Identity=22.9235880398671, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=647, Percent_Identity=32.7666151468315, Blast_Score=276, Evalue=7e-75,
Organism=Saccharomyces cerevisiae, GI6323011, Length=259, Percent_Identity=28.957528957529, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6321807, Length=253, Percent_Identity=26.4822134387352, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324256, Length=192, Percent_Identity=27.0833333333333, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=262, Percent_Identity=25.1908396946565, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24584738, Length=618, Percent_Identity=36.084142394822, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI17136276, Length=264, Percent_Identity=29.5454545454545, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640849, Length=254, Percent_Identity=25.9842519685039, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24640851, Length=254, Percent_Identity=25.9842519685039, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 67758; Mature: 67627

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMRTEYCGLVTEHLLGQTVSLCGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCHHHHHH
TAEGVRNEFCVQIKGLVRNRPDGTVNAGLKSGRIEVLCHELNVLNASVTPPFQLDDDNLS
HHHHHCHHHHHEEHHHHCCCCCCCEECCCCCCCEEEEEEEHHEEECCCCCCCEECCCCCH
ETTRLTHRVLDLRRPQMQHNLRLRYRVAIEARKYLDEQGFIDIETPMLTKSTPEGARDYL
HHHHHHHHHHHHCCCHHHCCCEEEEEEEEHHHHHCCCCCCEEECCCCCCCCCCCCCHHCC
VPSRVNAGQFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETS
CCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCHH
FLGEQEIRDLFEDMIRHIFKTTIDVELDAKFPVMPYSEAMARFGSDKPDLRVQLEFTELT
HCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHH
DAMKDVDFKVFSTPANAKDGRVAALRVPKGGELSRGDIDGYTEFVRIYGAKGLAWIKVNE
HHHHHCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECC
KAKGRDGLQSPIVKNLHDASIAAILERTGAEDGDIIFFAADRAKVVNDSLGALRLKIGHS
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCCEEEEEECCC
EFGKANGLVQAGWKPLWVVDFPMFEYDDEDARYVAAHHPFTSPKDEHLEYLETDPGRCLA
CCCCCCCEEECCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCHHHH
KAYDMVLNGWEIGGGSVRIHREEVQSKVFRALKIGAEEAQLKFGFLLDALQYGAPPHGGI
HHHHHHHCCEEECCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCH
AFGLDRIVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSPVDERQLRELHIRLRQPEQPKA
HHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SMRTEYCGLVTEHLLGQTVSLCGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCHHHHHH
TAEGVRNEFCVQIKGLVRNRPDGTVNAGLKSGRIEVLCHELNVLNASVTPPFQLDDDNLS
HHHHHCHHHHHEEHHHHCCCCCCCEECCCCCCCEEEEEEEHHEEECCCCCCCEECCCCCH
ETTRLTHRVLDLRRPQMQHNLRLRYRVAIEARKYLDEQGFIDIETPMLTKSTPEGARDYL
HHHHHHHHHHHHCCCHHHCCCEEEEEEEEHHHHHCCCCCCEEECCCCCCCCCCCCCHHCC
VPSRVNAGQFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETS
CCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCHH
FLGEQEIRDLFEDMIRHIFKTTIDVELDAKFPVMPYSEAMARFGSDKPDLRVQLEFTELT
HCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHH
DAMKDVDFKVFSTPANAKDGRVAALRVPKGGELSRGDIDGYTEFVRIYGAKGLAWIKVNE
HHHHHCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECC
KAKGRDGLQSPIVKNLHDASIAAILERTGAEDGDIIFFAADRAKVVNDSLGALRLKIGHS
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCCEEEEEECCC
EFGKANGLVQAGWKPLWVVDFPMFEYDDEDARYVAAHHPFTSPKDEHLEYLETDPGRCLA
CCCCCCCEEECCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCHHHH
KAYDMVLNGWEIGGGSVRIHREEVQSKVFRALKIGAEEAQLKFGFLLDALQYGAPPHGGI
HHHHHHHCCEEECCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCH
AFGLDRIVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSPVDERQLRELHIRLRQPEQPKA
HHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA