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The map label for this gene is dacB [H]

Identifier: 116690639

GI number: 116690639

Start: 2901089

End: 2902621

Strand: Direct

Name: dacB [H]

Synonym: Bcen2424_2619

Alternate gene names: 116690639

Gene position: 2901089-2902621 (Clockwise)

Preceding gene: 116690632

Following gene: 116690640

Centisome position: 83.27

GC content: 70.71

Gene sequence:

>1533_bases
ATGCCGATTTCCGATCTTTCCGCCCGGTTCGTCCCTCGCGCCCGCGCCTGCCTGCGGGCGGCCGCCGTCGTCGCCACCTG
CACGGCCCTCGCGTTCGCGCCGTCCGCGCAGGCCCGCAAGAAATCCCACAAGGAAGCGCGCAAGACCGCCGTCGTGTCGC
CCGCCGCGCGCGCGGCCGGGCTACCGGCCTCCGTGCTCGTCGCGCTGCAGCGCGCGAAGGTGCCGGCATCGGCGATGAGC
GTCGTGGTCGAGCGCGTCGGCGATCCGCAACCGCTGATCGCATGGAATGCGAGCCGGCCGATGCTGCCGGCCTCGACGAT
GAAGCTCGTGACGACCTTCTCGGGCCTGTCGATCCTCGGCCCCGACTACCGCTGGCGCACCACCGCGTACACGGACGGCA
CGATCGACCCCGACGGCACGCTGCAGGGCAACCTGTACATCAAGGGCACCGGCGATCCGAAGCTCGTGCCCGAGGAGCTG
ATCGACCTCGTCGACAAGCTGCGCAAGGCCGGCGTCAAGCGCGTGGCCGGCGGCCTCGTGCTCGACAAGAGCTACTTCGC
GGCGTCGACGCGCGACCTGCCGTCGTTCGACGACGATGCCAGCGCGCCGTACAACGTCGGCCCCGATCCGCTGCTGTACG
CGTTCAAGGCCGTGTCGTTCACGGTCACGCCGGGCGAGGACGGCAAGGTCGCCGTCGACGTGCTGCCGCCGCTCGCGGAT
CTGTCGATCGACAACCAGCTCGTCGAAGGCTCGGGCTCGTGCAGCGCGGCCGCGGCGGCCGCGCGCCCGACGCTGACGGC
CGGCGGCGGGATCATGACCGCGTCGTTCGCCGGCGACTACCCGCTGCGCTGCGGCGCGCACACGACCAACCTCGCGATCC
TCGATCACACGACCTTCTTCGCGCGCGGCTTCCTCGCGCTGTGGCAGCAGGACGGCGGCACGATCGCGGGGCCGGTGAGC
GAAGGCAAGGTGCCGACCACCGCGCGGCCGCTCGCCGTGCATCACAGCCCGGTGCTCGGCAGCATCGTCTACGACATCAA
CAAGTTCAGCAACAACGTGATGGCGCGCAACCTGTTCCTGACGATCGGCGCGGTCAGCGGCAAGCCGCCCGCGACACCCG
AGCAGTCGAGCCGCGCGATCCAGGCGTTCCTGCAGAAGAACGGCATCGCGATGCCCGACCTCGTGCTCGAGAACGGCTCG
GGGCTGTCGCGCGAAGAGCACGTGAGCGCGCTGTCGCTCGCCGCGCTGCTGCAGGCCGCGAATGCGAGCCCCGTCGCGCA
GGCGTTCGTCGATTCGCTGCCGATCGCCGGCATCGACGGCACGATGAAGAACCGCCTCACCAACGCGGGCGTGCTCGGCA
ACGCGCACATCAAGACCGGCACGCTGCGCGACGTGCGGGCGATCGCCGGCTACGTCGCCGGCGCCGACGGCCAGAGCTAC
ATCGTCGTCAGCTTCATCAACAACGATCACGCGGAAGGCGCGCGCGCCGCGCACGACACGCTGCTCGAGTGGGTCTACGC
GGGCGCGCACTGA

Upstream 100 bases:

>100_bases
AAGGCGGATTGCAAGGGGCCTGCCCCGACTTGTCTCTACAATGTGCGCTTCGGCGCCCCCGCGCGTCCTGCCTGAGCCCC
TGCTTTTCCCGTATTCGCCC

Downstream 100 bases:

>100_bases
CGGTGCGCGCACCGTGCATCGGGCCGCTCTGACAGGGCGGCCCGTGCGACTTCCCGCACCGCGCCGCCCGCGAAATCCTG
CGCCGCGCGGTTTGAAACGC

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MPISDLSARFVPRARACLRAAAVVATCTALAFAPSAQARKKSHKEARKTAVVSPAARAAGLPASVLVALQRAKVPASAMS
VVVERVGDPQPLIAWNASRPMLPASTMKLVTTFSGLSILGPDYRWRTTAYTDGTIDPDGTLQGNLYIKGTGDPKLVPEEL
IDLVDKLRKAGVKRVAGGLVLDKSYFAASTRDLPSFDDDASAPYNVGPDPLLYAFKAVSFTVTPGEDGKVAVDVLPPLAD
LSIDNQLVEGSGSCSAAAAAARPTLTAGGGIMTASFAGDYPLRCGAHTTNLAILDHTTFFARGFLALWQQDGGTIAGPVS
EGKVPTTARPLAVHHSPVLGSIVYDINKFSNNVMARNLFLTIGAVSGKPPATPEQSSRAIQAFLQKNGIAMPDLVLENGS
GLSREEHVSALSLAALLQAANASPVAQAFVDSLPIAGIDGTMKNRLTNAGVLGNAHIKTGTLRDVRAIAGYVAGADGQSY
IVVSFINNDHAEGARAAHDTLLEWVYAGAH

Sequences:

>Translated_510_residues
MPISDLSARFVPRARACLRAAAVVATCTALAFAPSAQARKKSHKEARKTAVVSPAARAAGLPASVLVALQRAKVPASAMS
VVVERVGDPQPLIAWNASRPMLPASTMKLVTTFSGLSILGPDYRWRTTAYTDGTIDPDGTLQGNLYIKGTGDPKLVPEEL
IDLVDKLRKAGVKRVAGGLVLDKSYFAASTRDLPSFDDDASAPYNVGPDPLLYAFKAVSFTVTPGEDGKVAVDVLPPLAD
LSIDNQLVEGSGSCSAAAAAARPTLTAGGGIMTASFAGDYPLRCGAHTTNLAILDHTTFFARGFLALWQQDGGTIAGPVS
EGKVPTTARPLAVHHSPVLGSIVYDINKFSNNVMARNLFLTIGAVSGKPPATPEQSSRAIQAFLQKNGIAMPDLVLENGS
GLSREEHVSALSLAALLQAANASPVAQAFVDSLPIAGIDGTMKNRLTNAGVLGNAHIKTGTLRDVRAIAGYVAGADGQSY
IVVSFINNDHAEGARAAHDTLLEWVYAGAH
>Mature_509_residues
PISDLSARFVPRARACLRAAAVVATCTALAFAPSAQARKKSHKEARKTAVVSPAARAAGLPASVLVALQRAKVPASAMSV
VVERVGDPQPLIAWNASRPMLPASTMKLVTTFSGLSILGPDYRWRTTAYTDGTIDPDGTLQGNLYIKGTGDPKLVPEELI
DLVDKLRKAGVKRVAGGLVLDKSYFAASTRDLPSFDDDASAPYNVGPDPLLYAFKAVSFTVTPGEDGKVAVDVLPPLADL
SIDNQLVEGSGSCSAAAAAARPTLTAGGGIMTASFAGDYPLRCGAHTTNLAILDHTTFFARGFLALWQQDGGTIAGPVSE
GKVPTTARPLAVHHSPVLGSIVYDINKFSNNVMARNLFLTIGAVSGKPPATPEQSSRAIQAFLQKNGIAMPDLVLENGSG
LSREEHVSALSLAALLQAANASPVAQAFVDSLPIAGIDGTMKNRLTNAGVLGNAHIKTGTLRDVRAIAGYVAGADGQSYI
VVSFINNDHAEGARAAHDTLLEWVYAGAH

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=451, Percent_Identity=26.8292682926829, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 53107; Mature: 52976

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPISDLSARFVPRARACLRAAAVVATCTALAFAPSAQARKKSHKEARKTAVVSPAARAAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHCC
LPASVLVALQRAKVPASAMSVVVERVGDPQPLIAWNASRPMLPASTMKLVTTFSGLSILG
CCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEC
PDYRWRTTAYTDGTIDPDGTLQGNLYIKGTGDPKLVPEELIDLVDKLRKAGVKRVAGGLV
CCCCEEEEEEECCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCEE
LDKSYFAASTRDLPSFDDDASAPYNVGPDPLLYAFKAVSFTVTPGEDGKVAVDVLPPLAD
EECHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEEEEEECCCCCCCEEEEECCCCCC
LSIDNQLVEGSGSCSAAAAAARPTLTAGGGIMTASFAGDYPLRCGAHTTNLAILDHTTFF
CCCCCCEECCCCCCCHHHHHCCCCEECCCCEEEEECCCCCCEECCCCCCEEEEEECHHHH
ARGFLALWQQDGGTIAGPVSEGKVPTTARPLAVHHSPVLGSIVYDINKFSNNVMARNLFL
HHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEEEE
TIGAVSGKPPATPEQSSRAIQAFLQKNGIAMPDLVLENGSGLSREEHVSALSLAALLQAA
EEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHC
NASPVAQAFVDSLPIAGIDGTMKNRLTNAGVLGNAHIKTGTLRDVRAIAGYVAGADGQSY
CCCHHHHHHHHHCCCCCCCCHHHHHHCCCCEECCCEEECCCHHHHHHHHHHHCCCCCCCE
IVVSFINNDHAEGARAAHDTLLEWVYAGAH
EEEEEECCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PISDLSARFVPRARACLRAAAVVATCTALAFAPSAQARKKSHKEARKTAVVSPAARAAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHCC
LPASVLVALQRAKVPASAMSVVVERVGDPQPLIAWNASRPMLPASTMKLVTTFSGLSILG
CCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEC
PDYRWRTTAYTDGTIDPDGTLQGNLYIKGTGDPKLVPEELIDLVDKLRKAGVKRVAGGLV
CCCCEEEEEEECCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCEE
LDKSYFAASTRDLPSFDDDASAPYNVGPDPLLYAFKAVSFTVTPGEDGKVAVDVLPPLAD
EECHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEEEEEECCCCCCCEEEEECCCCCC
LSIDNQLVEGSGSCSAAAAAARPTLTAGGGIMTASFAGDYPLRCGAHTTNLAILDHTTFF
CCCCCCEECCCCCCCHHHHHCCCCEECCCCEEEEECCCCCCEECCCCCCEEEEEECHHHH
ARGFLALWQQDGGTIAGPVSEGKVPTTARPLAVHHSPVLGSIVYDINKFSNNVMARNLFL
HHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEEEE
TIGAVSGKPPATPEQSSRAIQAFLQKNGIAMPDLVLENGSGLSREEHVSALSLAALLQAA
EEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHC
NASPVAQAFVDSLPIAGIDGTMKNRLTNAGVLGNAHIKTGTLRDVRAIAGYVAGADGQSY
CCCHHHHHHHHHCCCCCCCCHHHHHHCCCCEECCCEEECCCHHHHHHHHHHHCCCCCCCE
IVVSFINNDHAEGARAAHDTLLEWVYAGAH
EEEEEECCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]