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The map label for this gene is pbuE [H]

Identifier: 116690608

GI number: 116690608

Start: 2865542

End: 2866765

Strand: Reverse

Name: pbuE [H]

Synonym: Bcen2424_2588

Alternate gene names: 116690608

Gene position: 2866765-2865542 (Counterclockwise)

Preceding gene: 116690609

Following gene: 116690603

Centisome position: 82.29

GC content: 73.12

Gene sequence:

>1224_bases
ATGAGCGAGTGCGTATCCGTCTCCGACCGCGCTGTCGATCGTGCGGCAGATTCGTCCGCGCCGGCTCGTGCACGTCTGCC
CGTCGCGAACCTGTTCGCGCTGGCCACCGCCGGCTTCATCACGGTGCTCACCGAAGCGTTGCCGGCCGGCCTGCTGCCGC
TGATGAGCGCCGACCTGCACGTGACCGAGGCGCTGATCGGTCAACTGGTGACCGTCTACGCGCTGGGTTCGATCGTCGCG
GCGATCCCGCTCGTCGCCGCGACGCGTGCGATGCGCCGGCGCCGGTTGCTGCTCGCGGCGCTGGCCGGCTTCGTCGTGTC
GAATGCGCTGACGGCCGCGTCGCCGTACTACGCGCTCACGCTGGCCGCGCGCTTCGTCGCGGGGATGTCGGCCGGGCTGC
TGTGGGCGCTGCTGGCGGGCTACGCGAGCCGGCTGGTCGACCCGTCGCTGCGCGGCAGGGCGATCGCGGTCGCGATGCTC
GGCATACCGGCAGCGATGTCGATCGGCATTCCGGCCGGCACGGCGCTCGGCGCGATGTTCGGCTGGCGCGTCGCGTTCGC
CGGGATCACGGTCGCGGCGCTGGCGCTGATCGGCTGGATTCGCCTGCGCGTGCCCGACTATCCGGGCCAGCCGGCCGGCG
CGCGCGAGCCGGTGCTCGGCGTGATGGCGCTGCCCGGCGTGCGGCCGGTGCTGACGGTGATGTTCGCGTACGTGCTCGCG
CACAACATGCTGTACACGTACGTCGCGCCGTTCCTTGCGGGCGTGCGGATGGGCGCGCAGGTCGATGTGGTGCTGTTCGT
GTTCGGCGTCGCGTCCCTCGTCGGCATCGCGCTGACGGGTGCATGGATCGGCGTCGCGCAGCGGCGGCTGACGCTCGCGA
GCATCGCGCTGTTCGCATTGGCGGCGGCGATGCTCGGCGCCGGGTCGGGGATGACGTTCGTCTATGCGGGCGTCGTGCTG
TGGGGGCTCGCGTTCGGCGGCGCGCCGACGCTGTTCCAGACGGCCACCGCGAACGCGGCGGGGGAGTCGGCCGACGTCGC
GCAGTCGATGCTGGTGACGGTCTGGAATCTCGCGATCGCCGGCGGTGGCATCGCGGGCGGGATGCTGCTCGGCGCGACCG
GCGCCGGCGCGATTCCGTGGGTGCTCGTCGCGCTGCTGGCGCTGGCGTGGGTCGGCGCGTGGCGCGCACGCCGCCATGCG
TTTCCGGCGCCGCGCGCGGCCTGA

Upstream 100 bases:

>100_bases
CGGTCGTCGCACTGACCAATACGTTATACGAAGGGATGCACGGGCGCATCGTCACCGACGTGCGCGATGCGGTGTATGGC
ATCGACGACCGGAGCGCGGC

Downstream 100 bases:

>100_bases
ACCCGTGCGGGCGACGGCCGCGTCAGGCCGCCTTCGCGGCGAGCGCGGCGCGCGCGCAGGCTTCGTCGGTGACGAGCCCC
GACAGCCAGCCGCCGCGCAG

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSECVSVSDRAVDRAADSSAPARARLPVANLFALATAGFITVLTEALPAGLLPLMSADLHVTEALIGQLVTVYALGSIVA
AIPLVAATRAMRRRRLLLAALAGFVVSNALTAASPYYALTLAARFVAGMSAGLLWALLAGYASRLVDPSLRGRAIAVAML
GIPAAMSIGIPAGTALGAMFGWRVAFAGITVAALALIGWIRLRVPDYPGQPAGAREPVLGVMALPGVRPVLTVMFAYVLA
HNMLYTYVAPFLAGVRMGAQVDVVLFVFGVASLVGIALTGAWIGVAQRRLTLASIALFALAAAMLGAGSGMTFVYAGVVL
WGLAFGGAPTLFQTATANAAGESADVAQSMLVTVWNLAIAGGGIAGGMLLGATGAGAIPWVLVALLALAWVGAWRARRHA
FPAPRAA

Sequences:

>Translated_407_residues
MSECVSVSDRAVDRAADSSAPARARLPVANLFALATAGFITVLTEALPAGLLPLMSADLHVTEALIGQLVTVYALGSIVA
AIPLVAATRAMRRRRLLLAALAGFVVSNALTAASPYYALTLAARFVAGMSAGLLWALLAGYASRLVDPSLRGRAIAVAML
GIPAAMSIGIPAGTALGAMFGWRVAFAGITVAALALIGWIRLRVPDYPGQPAGAREPVLGVMALPGVRPVLTVMFAYVLA
HNMLYTYVAPFLAGVRMGAQVDVVLFVFGVASLVGIALTGAWIGVAQRRLTLASIALFALAAAMLGAGSGMTFVYAGVVL
WGLAFGGAPTLFQTATANAAGESADVAQSMLVTVWNLAIAGGGIAGGMLLGATGAGAIPWVLVALLALAWVGAWRARRHA
FPAPRAA
>Mature_406_residues
SECVSVSDRAVDRAADSSAPARARLPVANLFALATAGFITVLTEALPAGLLPLMSADLHVTEALIGQLVTVYALGSIVAA
IPLVAATRAMRRRRLLLAALAGFVVSNALTAASPYYALTLAARFVAGMSAGLLWALLAGYASRLVDPSLRGRAIAVAMLG
IPAAMSIGIPAGTALGAMFGWRVAFAGITVAALALIGWIRLRVPDYPGQPAGAREPVLGVMALPGVRPVLTVMFAYVLAH
NMLYTYVAPFLAGVRMGAQVDVVLFVFGVASLVGIALTGAWIGVAQRRLTLASIALFALAAAMLGAGSGMTFVYAGVVLW
GLAFGGAPTLFQTATANAAGESADVAQSMLVTVWNLAIAGGGIAGGMLLGATGAGAIPWVLVALLALAWVGAWRARRHAF
PAPRAA

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=379, Percent_Identity=29.287598944591, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1787808, Length=222, Percent_Identity=27.027027027027, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787947, Length=260, Percent_Identity=31.9230769230769, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1786595, Length=350, Percent_Identity=26.2857142857143, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41470; Mature: 41339

Theoretical pI: Translated: 11.31; Mature: 11.31

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSECVSVSDRAVDRAADSSAPARARLPVANLFALATAGFITVLTEALPAGLLPLMSADLH
CCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
VTEALIGQLVTVYALGSIVAAIPLVAATRAMRRRRLLLAALAGFVVSNALTAASPYYALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LAARFVAGMSAGLLWALLAGYASRLVDPSLRGRAIAVAMLGIPAAMSIGIPAGTALGAMF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHCCCCCHHHHHHHH
GWRVAFAGITVAALALIGWIRLRVPDYPGQPAGAREPVLGVMALPGVRPVLTVMFAYVLA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHH
HNMLYTYVAPFLAGVRMGAQVDVVLFVFGVASLVGIALTGAWIGVAQRRLTLASIALFAL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAMLGAGSGMTFVYAGVVLWGLAFGGAPTLFQTATANAAGESADVAQSMLVTVWNLAIA
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
GGGIAGGMLLGATGAGAIPWVLVALLALAWVGAWRARRHAFPAPRAA
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SECVSVSDRAVDRAADSSAPARARLPVANLFALATAGFITVLTEALPAGLLPLMSADLH
CCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
VTEALIGQLVTVYALGSIVAAIPLVAATRAMRRRRLLLAALAGFVVSNALTAASPYYALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LAARFVAGMSAGLLWALLAGYASRLVDPSLRGRAIAVAMLGIPAAMSIGIPAGTALGAMF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHCCCCCHHHHHHHH
GWRVAFAGITVAALALIGWIRLRVPDYPGQPAGAREPVLGVMALPGVRPVLTVMFAYVLA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHH
HNMLYTYVAPFLAGVRMGAQVDVVLFVFGVASLVGIALTGAWIGVAQRRLTLASIALFAL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAMLGAGSGMTFVYAGVVLWGLAFGGAPTLFQTATANAAGESADVAQSMLVTVWNLAIA
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
GGGIAGGMLLGATGAGAIPWVLVALLALAWVGAWRARRHAFPAPRAA
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]