Click here to switch to the map view.

The map label for this gene is mdlC [H]

Identifier: 116690603

GI number: 116690603

Start: 2858670

End: 2860277

Strand: Reverse

Name: mdlC [H]

Synonym: Bcen2424_2583

Alternate gene names: 116690603

Gene position: 2860277-2858670 (Counterclockwise)

Preceding gene: 116690608

Following gene: 116690602

Centisome position: 82.1

GC content: 72.39

Gene sequence:

>1608_bases
ATGTCCGGCACCCCGTCCCAATCCGCCGCACCCGTCACGGTCCGCGACGCCGTGATCGACCTGTTCCGCCAGTTCGGCAT
CGACCGCGTGTTCGGCAACCCCGGCTCGACCGAGCTGCCGATGTTCCGCGACTTCCCCGCCGATTTCCGCTACGTGCTCG
GCCTGCAGGAGGCCGTCGTGGTCGGGATGGCCGACGGTCACGCGCAGGCCACCGGCAATGCGGCGGTCGTGAACCTGCAT
TCGGCGGCGGGCGTCGGCAACGCGATGGGCAATCTCTTCACCGCGTTCAAGAACCGCTCGCCGCTGATCGTCACCGCGGG
CCAGCAGGCGCGCGCGATTCTGCCGTTCGATCCGTTCCTCGGCGCGACGCAGGCCGCCGAGCTGCCGAAGCCGTACGTGA
AATGGAGCATCGAGCCGGCGCGCGCGCAGGACGTGCCGGCCGCGATCGCGCGGGCGTACCGCATCGCGATGCAGGAGCCG
CGCGGCCCCGTGTTCGTGTCGATTCCGGTCGACGACTGGGATCAGCCGGCCGAATTGCTGCCGCGCCGTGACGTGAGCAG
CATCGTGCGGCCCGATCCCGACGCGCTTGCGCGACTTGGCGACGCGCTCGACGCGGCGCGCCGCCCGGCGTTCGTGGTCG
GCGCGGCCGTCGATCGCGCGGGCGCGTGGGACGACGTCGTGCGCGTCGCCGAGCGGCACCGTGCGCGCGTGTACGTCGCG
CCGATGTCGGGGCGCTGCAGTTTTCCGGAGGATCATCCGCTGTTCGCCGGCTTCCTGCCCGCGATCCGCGAGAAGATCGT
CGCGCGGCTCGACGGGCACGATCTCGTGTTCGCGTTCGGCGCGCCTGCGTTCACCTATCACATCGAGGGCTTCGGCCCGC
ACGTGCCGCCGGGCGCGACGCTCGTGCAGCTCGTCGACGATCCGGGCGTCGCCGCGTGGACGCCGTCGGGCGACGCGGTG
GTCGGCAATTTGCGGCTCGCCGCGCGCGATCTGCTGGCGCGGCCCGCGCCGCCCGAGCGGCCGATGCCCGCGCCGCGCGC
GCCGCGGCTGCGCGTCGACGCGCCTGCTGCCGGCGAACGCATGTCGGTCGCGTTCGCGCTGCAGACGCTCGCCGACGTGC
GCGACGCGCACGACATCGTCGTCGAGGAGGCGCCGAGCGCGCGGCCGGTGATGCAGGAACATCTGCCGTTCACGCGCAGC
GGCACGTTCTACACGATGGACAGCGGCGGGCTGGGCTACGGGATGCCGGCCGCCGTCGGCGTCGCGCTCGCGCAGCCGGG
CCGACGCGTGATCGCGCTGATCGGCGACGGCTCGAGCCTGTATTCGATCCAGGCGCTGTGGAGCGCCGCGCAGTTGAAGC
TGCCGATCACGTTCGTGATCCTGAACAACCGGCGTTACGCGGCGCTGCAGGATTTCGCGCCCGTGTTCGGCTTCGGCCCC
GGCGACCCGGTGCAAGGCACCGATCTGCCCGACCTGGATTTCGTCGCGCTCGCGCAAGGGATGGGCTGTCGCGGCGTGCG
CGTCGCCGAAGCGGCGAGGCTGCGCGACGCGCTGACCGACGCGCTGCGCGCGGCGACGCCGGTCGTCGTCGAAGTCGAGA
TTGCGTGA

Upstream 100 bases:

>100_bases
TGCTGGAACGTAATGGCTTTGCGCAAAAAATACAGTATCCGTGAATGGCTTGCGCTTCGATACTGTGGAAACCGCCAAGC
CATCGAGGAGACGCCCGAGC

Downstream 100 bases:

>100_bases
CGCGCCGCGCGCGTCTTTCCGGAGATTCCATGCAAACCGTAGCGATGCTGATCGGCGGCGAACGCCGCCACTCGTCCGGC
GGCGCGACCTTCGTGCGCCG

Product: benzoylformate decarboxylase

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MSGTPSQSAAPVTVRDAVIDLFRQFGIDRVFGNPGSTELPMFRDFPADFRYVLGLQEAVVVGMADGHAQATGNAAVVNLH
SAAGVGNAMGNLFTAFKNRSPLIVTAGQQARAILPFDPFLGATQAAELPKPYVKWSIEPARAQDVPAAIARAYRIAMQEP
RGPVFVSIPVDDWDQPAELLPRRDVSSIVRPDPDALARLGDALDAARRPAFVVGAAVDRAGAWDDVVRVAERHRARVYVA
PMSGRCSFPEDHPLFAGFLPAIREKIVARLDGHDLVFAFGAPAFTYHIEGFGPHVPPGATLVQLVDDPGVAAWTPSGDAV
VGNLRLAARDLLARPAPPERPMPAPRAPRLRVDAPAAGERMSVAFALQTLADVRDAHDIVVEEAPSARPVMQEHLPFTRS
GTFYTMDSGGLGYGMPAAVGVALAQPGRRVIALIGDGSSLYSIQALWSAAQLKLPITFVILNNRRYAALQDFAPVFGFGP
GDPVQGTDLPDLDFVALAQGMGCRGVRVAEAARLRDALTDALRAATPVVVEVEIA

Sequences:

>Translated_535_residues
MSGTPSQSAAPVTVRDAVIDLFRQFGIDRVFGNPGSTELPMFRDFPADFRYVLGLQEAVVVGMADGHAQATGNAAVVNLH
SAAGVGNAMGNLFTAFKNRSPLIVTAGQQARAILPFDPFLGATQAAELPKPYVKWSIEPARAQDVPAAIARAYRIAMQEP
RGPVFVSIPVDDWDQPAELLPRRDVSSIVRPDPDALARLGDALDAARRPAFVVGAAVDRAGAWDDVVRVAERHRARVYVA
PMSGRCSFPEDHPLFAGFLPAIREKIVARLDGHDLVFAFGAPAFTYHIEGFGPHVPPGATLVQLVDDPGVAAWTPSGDAV
VGNLRLAARDLLARPAPPERPMPAPRAPRLRVDAPAAGERMSVAFALQTLADVRDAHDIVVEEAPSARPVMQEHLPFTRS
GTFYTMDSGGLGYGMPAAVGVALAQPGRRVIALIGDGSSLYSIQALWSAAQLKLPITFVILNNRRYAALQDFAPVFGFGP
GDPVQGTDLPDLDFVALAQGMGCRGVRVAEAARLRDALTDALRAATPVVVEVEIA
>Mature_534_residues
SGTPSQSAAPVTVRDAVIDLFRQFGIDRVFGNPGSTELPMFRDFPADFRYVLGLQEAVVVGMADGHAQATGNAAVVNLHS
AAGVGNAMGNLFTAFKNRSPLIVTAGQQARAILPFDPFLGATQAAELPKPYVKWSIEPARAQDVPAAIARAYRIAMQEPR
GPVFVSIPVDDWDQPAELLPRRDVSSIVRPDPDALARLGDALDAARRPAFVVGAAVDRAGAWDDVVRVAERHRARVYVAP
MSGRCSFPEDHPLFAGFLPAIREKIVARLDGHDLVFAFGAPAFTYHIEGFGPHVPPGATLVQLVDDPGVAAWTPSGDAVV
GNLRLAARDLLARPAPPERPMPAPRAPRLRVDAPAAGERMSVAFALQTLADVRDAHDIVVEEAPSARPVMQEHLPFTRSG
TFYTMDSGGLGYGMPAAVGVALAQPGRRVIALIGDGSSLYSIQALWSAAQLKLPITFVILNNRRYAALQDFAPVFGFGPG
DPVQGTDLPDLDFVALAQGMGCRGVRVAEAARLRDALTDALRAATPVVVEVEIA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=442, Percent_Identity=23.0769230769231, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI87081685, Length=543, Percent_Identity=27.255985267035, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1790104, Length=553, Percent_Identity=24.4122965641953, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1786717, Length=561, Percent_Identity=25.1336898395722, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1787096, Length=505, Percent_Identity=22.970297029703, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=24.1007194244604, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323755, Length=554, Percent_Identity=24.9097472924188, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 56934; Mature: 56802

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTPSQSAAPVTVRDAVIDLFRQFGIDRVFGNPGSTELPMFRDFPADFRYVLGLQEAVV
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEE
VGMADGHAQATGNAAVVNLHSAAGVGNAMGNLFTAFKNRSPLIVTAGQQARAILPFDPFL
EEECCCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCCEECCCCCCC
GATQAAELPKPYVKWSIEPARAQDVPAAIARAYRIAMQEPRGPVFVSIPVDDWDQPAELL
CCCHHHHCCCCHHEEECCHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHC
PRRDVSSIVRPDPDALARLGDALDAARRPAFVVGAAVDRAGAWDDVVRVAERHRARVYVA
CCCCHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECHHCCCCHHHHHHHHHHCCCEEEEE
PMSGRCSFPEDHPLFAGFLPAIREKIVARLDGHDLVFAFGAPAFTYHIEGFGPHVPPGAT
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCCE
LVQLVDDPGVAAWTPSGDAVVGNLRLAARDLLARPAPPERPMPAPRAPRLRVDAPAAGER
EEEEECCCCCEEECCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCH
MSVAFALQTLADVRDAHDIVVEEAPSARPVMQEHLPFTRSGTFYTMDSGGLGYGMPAAVG
HHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHCCCCCCCCCEEEECCCCCCCCCHHHHH
VALAQPGRRVIALIGDGSSLYSIQALWSAAQLKLPITFVILNNRRYAALQDFAPVFGFGP
HHHCCCCCEEEEEEECCCCHHHHHHHHHHHEEECEEEEEEECCCCEEHHHHCCHHHCCCC
GDPVQGTDLPDLDFVALAQGMGCRGVRVAEAARLRDALTDALRAATPVVVEVEIA
CCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
>Mature Secondary Structure 
SGTPSQSAAPVTVRDAVIDLFRQFGIDRVFGNPGSTELPMFRDFPADFRYVLGLQEAVV
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEE
VGMADGHAQATGNAAVVNLHSAAGVGNAMGNLFTAFKNRSPLIVTAGQQARAILPFDPFL
EEECCCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCCEECCCCCCC
GATQAAELPKPYVKWSIEPARAQDVPAAIARAYRIAMQEPRGPVFVSIPVDDWDQPAELL
CCCHHHHCCCCHHEEECCHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHC
PRRDVSSIVRPDPDALARLGDALDAARRPAFVVGAAVDRAGAWDDVVRVAERHRARVYVA
CCCCHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECHHCCCCHHHHHHHHHHCCCEEEEE
PMSGRCSFPEDHPLFAGFLPAIREKIVARLDGHDLVFAFGAPAFTYHIEGFGPHVPPGAT
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCCE
LVQLVDDPGVAAWTPSGDAVVGNLRLAARDLLARPAPPERPMPAPRAPRLRVDAPAAGER
EEEEECCCCCEEECCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCH
MSVAFALQTLADVRDAHDIVVEEAPSARPVMQEHLPFTRSGTFYTMDSGGLGYGMPAAVG
HHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHCCCCCCCCCEEEECCCCCCCCCHHHHH
VALAQPGRRVIALIGDGSSLYSIQALWSAAQLKLPITFVILNNRRYAALQDFAPVFGFGP
HHHCCCCCEEEEEEECCCCHHHHHHHHHHHEEECEEEEEEECCCCEEHHHHCCHHHCCCC
GDPVQGTDLPDLDFVALAQGMGCRGVRVAEAARLRDALTDALRAATPVVVEVEIA
CCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2271624; 7663351; 9665697 [H]